iPSC to Hepatocyte Differentiation System Overview
The process of generating hiPS cell-derived hepatocytes begins with the directed differentiation of hiPS cells into definitive endoderm (DE) cells, which are then differentiated further into hepatocytes. The complete system provides media, supplements, and coating reagents for each step of the hiPS-cell-to-hepatocyte differentiation protocol. Starting with approximately 3 x 106 undifferentiated hiPS cells, this system yields 5 x 106 hepatocytes-equivalent to a confluent monolayer of 50 cm2. Importantly, this do-it-yourself system offers a solution for the consistent production of assay-ready cells from patient-derived cells, or from Cellartis brand iPS cell lines-enabling highly reproducible results.. Successful differentiation depends on the quality of the starting material; a homogeneous, undifferentiated stem cell population is ideal. The iPS Cell to Hepatocyte Differentiation System promotes high-quality starting material by ...http://www.clontech.com/US/Products/Stem_Cell_Research/Resources/iPS_to_Hepatocyte_Differentiation_Overview?sitex=10020:22372:US&PEBCL1=q8DIRG9bPvMbTPhbC49c9ONqG5&PEBCL1_pses=ZG7AFC907621D7D9B47296560AA304D443226DB8FD4546DE161C4122758BE6DAF83AA0A21DAD20AEFF0C559D6E161D0F78B40BF35311019719
OPC differentiation assay<...
Renovo's OPC differentiation assay is used to identify compounds that promote the production of oligodendrocytes from OPCs. In this assay, OPCs are cultured with or without compounds in differentiation media in 96-well plates. Following 5 days of differentiation, cells are stained and imaged in high-content ArrayScan™ reader in multiple channels. Computer algorithms are used to quantify the number of viable and pyknotic cells, and the number of EGFP+ oligodendrocytes in each well of the plate. Immunostaining of additional markers of different cell types can also be performed and quantified.. Applications of the OPC differentiation assay include:. ...http://www.renovoneural.com/services/cell-based-assays/opc-differentiation-assay/
v-jun oncogene prevents terminal differentiation and suppresses muscle-specific gene expression in ASV-17-infected muscle cells...
Infection of replicating quail myoblasts with avian sarcoma virus 17 (ASV-17) results in the inhibition of terminal differentiation into multinucleated myotubes and in the acquisition of anchorage-independent proliferation. Expression of v-jun, the ASV-17 oncogene, concomitantly leads to the accumulation of the gag-jun polyprotein P65 in the nucleus and to the lack of expression of typical differentiation-specific genes such as myosin heavy chain (MHC) and alpha-actinin. Surprisingly, expression of desmin, the muscle-specific subunit of intermediate filaments, is conserved in ASV-17-transformed myoblasts. Analysis of clonal strains of transformed myoblasts suggests that (i) suppression of morphological and biochemical differentiation depends on the absence of muscle-specific gene transcripts; (ii) inhibition of muscle differentiation by v-jun does not depend on the transcriptional silencing of MyoD, a muscle-specific regulatory gene; (iii) ...https://iris.uniroma1.it/handle/11573/247558
Abstract 18478: Deletion of E2f1 Promotes Bone-marrow Progenitor Cell Differentiation and Ischemic Cardiac Repair | Circulation
Background: We have previously shown that knockout of E2F1 in mice enhances angiogenesis following induction of hind limb ischemia. Recent studies suggest that suppression of E2F1 enhances oxidative phosphorylation in a variety of cell types. Since an increase in oxidative phosphorylation in stem/progenitor cells is often associated with cell differentiation, we hypothesize that E2F1-deficiency may promote bone marrow (BM) progenitor cell differentiation thereby impact on ischemic cardiac repair.. Methods and Results: We cultured bone marrow (BM) Lin- progenitor cells under hypoxic and normxic conditions for 24 h, then measured the expression of metabolism associated genes and evaluated cell proliferation and differentiation. We also performed adoptive BM transplantation to reconstitute BM of WT mice with E2F1-/- or WT BM, followed by surgical induction of myocardial infarction (MI), to compare the role of BM E2F1 in the cardiac repair in vivo. Notably, we ...http://circ.ahajournals.org/content/130/Suppl_2/A18478
Cutting edge: STAT1 is required for IL-6-mediated Bcl6 induction for early follicular helper cell differentiation. | CHAVI-ID
Bcl6 is required for CD4 T cell differentiation into T follicular helper cells (Tfh). In this study, we examined the role of IL-6 in early processes of in vivo Tfh differentiation, because the timing and mechanism of action of IL-6 in Tfh differentiation have been controversial in vivo. We found that early Bcl6(+)CXCR5(+) Tfh differentiation was severely impaired in the absence of IL-6; however, STAT3 deficiency failed to recapitulate that defect. IL-6R signaling activates the transcription factor STAT1 specifically in CD4 T cells. Strikingly, we found that STAT1 activity was required for Bcl6 induction and early Tfh differentiation in vivo. IL-6 mediated STAT3 activation is important for downregulation of IL-2Rα to limit Th1 cell differentiation in an acute viral infection. Thus, IL-6 signaling is a major early inducer of the Tfh differentiation program unexpectedly mediated by both STAT3 ...http://chavi-id.org/publications/cutting-edge-stat1-required-il-6-mediated-bcl6-induction-early-follicular-helper-cell
Functional characterization of Nurr1 and Pin1 in neuronal progenitor cell differentiation | ScholarBank@NUS
Nurr1, a transcription factor belonging to the nuclear receptor family, is essential for the generation of midbrain dopamine (DA) cellsduring embryonic development and it continues to be expressed in adult DA neurons. However, the mechanism by which Nurr1 promotes dopamine cell differentiation has remained unknown. In this study, I have used a neuronal progenitor cell line (NT2/D1), which retains some stem cell characteristics and is capable only of terminal differentiation into neurons, to analyze the function of Nurr1 in dopamine cell development. The results demonstrated that Nurr1 can induce cell cycle arrest and the cells differentiated with distinct neuronal morphology after all-trans retinoic acid treatment. It was also indicated that up-regulation of some dopaminergic neuron markers (e.g. TH, DAT and D2DR) while down-regulation of CyclinD1-Cdk6 activity marks the key events in the early stages of dopaminergic neuron differentiation. Furthermore, ...http://scholarbank.nus.edu.sg/handle/10635/15062
Ppdpf (untagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA...
Ppdpf (untagged) - Mouse pancreatic progenitor cell differentiation and proliferation factor homolog (zebrafish)RIKEN cDNA 2700038C09 gene (Ppdpf), (10ug), 10 µg.https://www.acris-antibodies.com/cdna/mouse-cdna/ppdpf-untagged-mouse-pancreatic-progenitor-cell-differentiation-and-proliferation-factor-homolog-zebrafish-riken-cdna-2700038c09-gene-ppdpf-10ug-mc205094.htm
Induced myeloid leukemia cell differentiation protein elisa and antibody
Shop Induced myeloid leukemia cell differentiation protein ELISA Kit, Recombinant Protein and Induced myeloid leukemia cell differentiation protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.https://www.mybiosource.com/protein_family.php?root=induced-myeloid-leukemia-cell-differentiation-protein
Integrins regulate epithelial cell differentiation by modulating Notch activity | Journal of Cell Science
The coordination of cell proliferation with the gradual differentiation of different cell types is essential for proper development and tissue homeostasis. However, little is known about the signals that couple cell cycle exit to differentiation switch during development. Here, we show that signaling mediated by integrins, the major cell-ECM receptors, contributes to the regulation of this switch in the posterior follicle cells of the Drosophila ovary. Furthermore, our experiments strongly suggest that one of the mechanisms by which integrins regulate epithelial cell differentiation is by modulating the activity of the Notch pathway through promoting the proper endosomal trafficking and/or processing of Notch.. Integrins are known to regulate cell differentiation and proliferation in other systems. The effects of particular integrins in regulating differentiation vary depending on the epithelial cell type. Thus, although β1 ...http://jcs.biologists.org/content/127/21/4667
Cell Lineage - QIAGEN
During embryonic development, pluripotent stem cells differentiate into 3 germ layers: ectoderm, mesoderm, and endoderm. These germ layers differentiate into multipotent stem cells (progenitors), which progress into terminally differentiated cells. Developmental processes require tightly regulated and carefully timed gene expression changes. The changes in gene expression are frequently regulated at the epigenetic level, in particular via DNA methylation. Analysis of specific cellular markers or activation of specific transcription factors involved in a differentiation process identify intermediately or terminally differentiated cells. In addition, cell lineage analyses can be used to study specific differentiation mechanisms. For example, expression of an early ectoderm marker in a neuron could denote that terminal differentiation is not complete, or a dysregulation of the differentiation program. Oncogenic cells often regress to an earlier ...https://www.qiagen.com/at/shop/genes-and-pathways/complete-biology-list/cell-lineage/
Cell Lineage - QIAGEN
During embryonic development, pluripotent stem cells differentiate into 3 germ layers: ectoderm, mesoderm, and endoderm. These germ layers differentiate into multipotent stem cells (progenitors), which progress into terminally differentiated cells. Developmental processes require tightly regulated and carefully timed gene expression changes. The changes in gene expression are frequently regulated at the epigenetic level, in particular via DNA methylation. Analysis of specific cellular markers or activation of specific transcription factors involved in a differentiation process identify intermediately or terminally differentiated cells. In addition, cell lineage analyses can be used to study specific differentiation mechanisms. For example, expression of an early ectoderm marker in a neuron could denote that terminal differentiation is not complete, or a dysregulation of the differentiation program. Oncogenic cells often regress to an earlier ...https://www.qiagen.com/ie/shop/genes-and-pathways/complete-biology-list/cell-lineage/
Pleiotrophin antagonizes Bromodomain-containing protein 2 (Brd2) during neuronal differentiation | Journal of Cell Science
Bromodomain-containing protein 2 (Brd2) is a BET family chromatin adaptor required for expression of cell cycle associated genes and therefore involved in cell cycle progression. Brd2 is expressed in proliferating neuronal progenitors, displays cell cycle-stimulating activity and, when overexpressed, impairs neuronal differentiation. Paradoxically, Brd2 is also detected in differentiating neurons. To shed light on the role of Brd2 in the transition from cell proliferation to differentiation we have looked for Brd2 interacting proteins upon induction of neuronal differentiation. Surprisingly, we have identified the growth factor Pleiotrophin (Ptn). Ptn antagonizes the cell cycle-stimulating activity associated with Brd2, thus enhancing induced neuronal differentiation. Moreover, Ptn knockdown reduces neuronal differentiation. Ptn-mediated antagonism of Brd2 has been assessed in a cell differentiation model ...http://jcs.biologists.org/content/early/2014/03/31/jcs.147462
TGF-β Signaling in Development | Science Signaling
The transforming growth factor-β (TGF-β) superfamily comprises nearly 30 growth and differentiation factors that include TGF-βs, activins, inhibins, and bone morphogenetic proteins (BMPs). Multiple members of the TGF-β superfamily serve key roles in stem cell fate commitment. The various members of the family can exhibit disparate roles in regulating the biology of embryonic stem (ES) cells and tumor suppression. For example, TGF-β inhibits proliferation of multipotent hematopoietic progenitors, promotes lineage commitment of neural precursors, and suppresses epithelial tumors. BMPs block neural differentiation of mouse and human ES cells, contribute to self-renewal of mouse ES cells, and also suppress tumorigenesis. ES cells and tumors may be exposed to multiple TGF-β members, and it is likely that the combination of growth factors and cross-talk among the intracellular signaling pathways is what precisely defines stem cell fate commitment. This Connections Map Pathway in ...http://stke.sciencemag.org/content/2007/399/cm1
Complex changes in the apoptotic and cell differentiation programs during initiation of the hair follicle response to...
Sigma-Aldrich offers abstracts and full-text articles by [Tatyana Y Sharova, Krzysztof Poterlowicz, Natalia V Botchkareva, Nikita A Kondratiev, Ahmar Aziz, Jeffrey H Spiegel, Vladimir A Botchkarev, Andrey A Sharov].http://www.sigmaaldrich.com/catalog/papers/24999588
IMSEAR at HELLIS: The Role of Twist1 in Stem Cell Differentiation through Mechanical Cues: A Review and Hypothesis.
Due to the pivotal role of stem cell differentiation in regeneration and disease cure, the study of it has always been a research highlight during the recent years. Stress microenvironment has a great impact on cell growth, proliferation, differentiation and apoptosis. Twist1, as a core epithelial-mesenchymal transition (EMT) regulatory factor, plays an important role in these processes. Moreover, Twist1 gene can express in alveolar bone - periodontal ligament interface and the expression can be regulated by changes in the occlusal force. In this article, we will present a review of Twist1 gene, especially in the aspect of the biological functions in stem cell differentiation under mechanical signals and explore whether Twist1 involved in tissue remodeling in alveolar bone - periodontal membrane interface under stress ...http://imsear.li.mahidol.ac.th/handle/123456789/183454
Pancreatic Differentiation of Patient-specific iPS Cells - Gene and Cell Therapy for Diabetes and Hypertensive Heart Disease...
Dr. Ikeda's lab has recently demonstrated successful differentiation of induced pluripotent stem (iPS) cells into glucose-responsive, insulin-producing cells in vitro.1 For a novel autologous iPS-mediated cell therapy for diabetes, there is a need to generate iPS cells from people with diabetes and differentiate derived cells into insulin-producing islet-like cells.. However, the feasibility of iPS generation from people with diabetes - or the pancreatic differentiation capability of derived iPS cells - remains elusive. The lab has recruited people with and without diabetes for iPS derivation and is currently analyzing their differentiation propensities and therapeutic effects in diabetic mouse models.. ...http://www.mayo.edu/research/labs/gene-cell-therapy-diabetes-hypertensive-heart-disease/pancreatic-differentiation-patient-specific-ips-cells
A new class of stem cells in South Africa: iPS cells
The key advantage of iPS cells over other stem cells is that they are patient-specific (and therefore immuno-compatible) and can be grown in infinite amounts. Moreover, they are not dogged by the ethical and religious controversies associated with hES cells, yet still have the same properties as hES cells. They also offer the possibility of conducting 'clinical-trials-in-the-dish', providing a platform for drug screening, disease modelling and gene/cell therapy in pre-clinical studies.3,4. How are iPS cells made?. When cell differentiation occurs, the cell follows a process of changes in gene activity whereby embryonic-specific genes are inactivated and differentiation-specific genes are activated. The end result of this differentiation 'programme' is a specialised cell of one type or another (e.g. cardiac muscle cells or neurons). To 'reprogramme' a fully differentiated adult cell into an iPS cell is surprisingly straightforward - all that is needed is ...http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0256-95742013000100012&lng=es&nrm=iso&tlng=en
The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells | Development
We have shown in this study that activation of the canonical Wnt pathway promoted, and inhibition of this pathway blocked, neuronal differentiation both in cortical NPC cultures and in the developing neocortex. We emphasize two aspects of these findings: (1) Wnts appear to function as an extracellular cue that instructively triggers neuronal differentiation; and (2) this effect of Wnts is dependent on the stage of development. In this Discussion, we address these aspects and their possible underlying mechanisms.. In general, two models can explain how the fate of an uncommitted precursor cell is influenced by extrinsic cues. In one model, extrinsic cues instruct multipotent precursor cells to commit to a particular lineage. In the other model, multipotent precursor cells choose their fate stochastically, and the proliferation and/or survival of specific lineage-restricted cells is then supported by extrinsic cues. For example, Pdgf treatment increases the size of the ...http://dev.biologists.org/content/131/12/2791
β-Catenin signaling is required for neural differentiation of embryonic stem cells | Development
We have found that β-catenin signaling and neural differentiation of ES cells are inhibited by culture at high density. This observation is consistent with prior studies of neural/neuronal differentiation of ES cells that have all used relatively low densities irrespective of whether EB or dissociated cell culture techniques were used (Gratsch and O'Shea, 2002; Ying et al., 2003). The need to culture the cells at low density to achieve neuronal differentiation limits the number of cells that could potentially be obtained for transplantation strategies and raises questions about the mechanisms mediating neuronal differentiation of the cells. Our studies suggest that β-catenin signaling promotes both neural and neuronal differentiation of ES cells, and that the effects of increased cell density are mediated at least in part by inhibition of β-catenin signaling.. Similar to observations with keratinocytes (Dietrich et al., ...http://dev.biologists.org/content/131/15/3545
Reduction of Prep1 Levels Affects Differentiation of Normal and Malignant B Cells and Accelerates Myc Driven Lymphomagenesis -...
Reduction of Prep1 Levels Affects Differentiation of Normal and Malignant B Cells and Accelerates Myc Driven Lymphomagenesis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.http://libros.duhnnae.com/2017/jun7/149818268845-Reduction-of-Prep1-Levels-Affects-Differentiation-of-Normal-and-Malignant-B-Cells-and-Accelerates-Myc-Driven-Lymphomagenesis.php
Movassagh M and Philpott A (2008), Cardiac differentiation in Xenopus requires the... - Xenbase Paper
AIMS: Cyclin-dependent kinase inhibitors (CDKIs) play a critical role in negatively regulating the proliferation of cardiomyocytes, although their role in cardiac differentiation remains largely undetermined. We have shown that the most prominent CDKI in Xenopus, p27(Xic1)(Xic1), plays a role in neuronal and myotome differentiation beyond its ability to arrest the cell cycle. Thus, we investigated whether it plays a similar role in cardiomyocyte differentiation. METHODS AND RESULTS: Xenopus laevis embryos were sectioned, and whole-mount antibody staining and immunofluorescence studies were carried out to determine the total number and percentage of differentiated cardiomyocytes in mitosis. Capped RNA and/or translation-blocking Xic1 morpholino antisense oligonucleotides (Xic1Mo) were microinjected into embryos, and their role on cardiac differentiation was assessed by in situ hybridization and/or PCR. We show that cell-cycling ...http://www.xenbase.org/literature/article.do?method=display&articleId=37634
A tumor-associated β1 integrin mutation that abrogates epithelial differentiation control | JCB
The T188I mutation we have identified is remarkable for three reasons. It is the first integrin mutation to be found in a human tumor. It is, unlike the majority of integrin disease mutations (Hogg and Bates, 2000), a gain of function mutation, increasing the affinity of β1 integrins for a variety of ligands. It impairs the ability of keratinocytes to undergo terminal differentiation and thus can contribute to the neoplastic phenotype.. The adhesion-promoting effect of the mutation was observed when T188I was expressed as α5β1 (Fig. 2 a; Fig. 3 c; Fig. 4 a), αvβ1 (Fig. 2 b), α3β1 or α6β1 (Fig. 3 d), and α2β1 (collagen receptor; Fig. 4 b) heterodimers. Nevertheless, the extent to which adhesion was activated did appear to depend on the α partner and the assay conditions (e.g., Figs. 2 and 3). By modeling the β1 I-like domain on the crystal structures of αvβ3 (Xiong et al., 2001, 2002), we can speculate about how the T188I mutation might increase ligand binding (Fig. 1 a). The ...http://jcb.rupress.org/content/160/4/589
springerlink, sciencedirect, wileyinterscience, z8OoqYUZwowyMHOpM6SRMRM1M0c: Three differentiation states risk-stratify bladder...
Current clinical judgment in bladder cancer (BC) relies primarily on pathological stage and grade. We investigated whether a molecular classification of tumor cell differentiation, based on a developmental biology approach, can provide additional prognostic information. Exploiting large preexisting gene-expression databases, we developed a biologically supervised computational model to predict markers that correspond with BC differentiation. To provide mechanistic insight, we assessed relative tumorigenicity and differentiation potential via xenotransplantation. We then correlated the prognostic utility of the identified markers to outcomes within gene expression and formalin-fixed paraffin-embedded (FFPE) tissue datasets. Our data indicate that BC can be subclassified into three subtypes, on the basis of their differentiation states: basal, intermediate, and differentiated, where only the most primitive tumor cell subpopulation within each ...http://journalaccess.blogspot.com/2012/02/three-differentiation-states-risk.html
detlaphiltdic: February 2008
BACKGROUND: Human embryonic stem cells (hESCs) offer a virtually unlimited source of neural cells for structural repair in neurological disorders, such as stroke. Neural cells can be derived from hESCs either by direct enrichment, or by isolating specific growth factor-responsive and expandable populations of human neural stem cells (hNSCs). Studies have indicated that the direct enrichment method generates a heterogeneous population of cells that may contain residual undifferentiated stem cells that could lead to tumor formation in vivo. METHODS/PRINCIPAL FINDINGS: We isolated an expandable and homogenous population of hNSCs (named SD56) from hESCs using a defined media supplemented with epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and leukemia inhibitory growth factor (LIF). These hNSCs grew as an adherent monolayer culture. They were fully neuralized and uniformly expressed molecular features of NSCs, including nestin, vimentin and radial glial markers. These hNSCs did ...http://detlaphiltdic.blogspot.com/2008/02/
3/26 Webinar: Tips and Techniques for Serum-free Expansion of hMSCs
There is a great interest in application of human mesenchymal stem cells (hMSCs) in cell therapy and tissue engineering due to their self-renewal, multi-lineage differentiation, immunomodulation, and trophic potential. One of the challenges faced in the clinical application of hMSCs is the need for efficient expansion of these cells in vitro without altering their capacity. Serum-free mammalian cell culture media, in particular, require optimization of the expansion protocols. Even subtle changes in routine handling can have a significant impact on the cells' potential. This seminar will cover the variables that can influence the desired regenerative and differentiation properties including medium selection, vessel surface treatment, impact of the cell source, and seeding density. We will also discuss how users can select the correct conditions for optimized growth and functionality. Biography ...https://www.corning.com/cala/es/products/life-sciences/news-events/events/2015/03/newsevent-66395.html
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