Frontiers | Five layers of receptor signalling in γδ T cell differentiation and activation | Immunology
The contributions of gamma-delta T cells to immunity to infection or tumours critically depend on their activation and differentiation into effectors capable of secreting cytokines and killing infected or transformed cells. These processes are molecularly controlled by surface receptors that capture key extracellular cues and convey downstream intracellular signals that regulate gamma-delta T cell physiology. The understanding of how environmental signals are integrated by gamma-delta T cells is critical for their manipulation in clinical settings. Here we discuss how different classes of surface receptors impact on human and murine gamma-delta T cell differentiation, activation and ...https://www.frontiersin.org/articles/10.3389/fimmu.2015.00015/full
"Bioactive food components that enhance gammadelta T cell function may " by Susan S. Percival, Jack F. Bukowski et al.
Gammadelta T cells are found largely within the epithelium and recognize antigens differently than their alphabeta T cell counterparts. TCR delta-/- knock out mice exhibit a rapid tumor onset, along with increased tumor incidence. Although limited, research demonstrates that nutrients and bioactive food components can influence gammadelta T cell cytotoxicity, cytokine secretion, and proliferative capacity, and the results are nonetheless intriguing. Among other functions, gammadelta T cells play a role in immunosurveillance against malignant cells, as shown by the T cell receptor (TCR)delta-/- knock out mice that exhibit a rapid tumor onset and increased tumor incidence. Some common dietary modifiers of ...http://escholarship.umassmed.edu/gsbs_sp/1424/
Some studies on human alpha-beta+ and gamma-delta+ T lymphocytes in health and disease | IRIS Università di Pisa
T Iymphocytes use the T cell receptor (TCR) to specifically recognize antigens. According to the type of TCR heterodimer, T cells can be phenotypically divided into alpha-beta+ or gamma-delta+ subsets. In many human inflammatory/autoimmune diseases significant expansions of ab+ and gd+ T cells have been described. Interestingly, in most of these diseases several microorganisms (including mycobacteria) are suspected as causative agents. In this thesis quantitative and qualitative studies of human T Iymphocytes, with particular interest in TCR V usage, were performed. Initially, the frequency and distribution of TCR ab+ and gd+ T Iymphocytes in healthy humans were studied. The existence of a site-specific TCR VB distribution pattern, different from that of peripheral blood, was demonstrated in the healthy human gut. Significantly higher gd+ T cell levels in peripheral blood ...https://arpi.unipi.it/handle/11568/50740
γ/δ T lymphocytes express CD40 ligand and induce isotype switching in B lymphocytes. | JEM
T cells expressing gamma/delta T cell receptors home to epithelial tissue and may play a role in immunity to infectious agents and foreign antigens. In an effort to understand the role of gamma/delta T cells in directing B cell responses, we investigated the capacity of human gamma/delta T cells to express CD40 ligand (CD40L) and to drive immunoglobulin (Ig) isotype switching in B cells. A multiple step purification procedure resulted in the recovery of highly pure populations of peripheral blood CD4-CD8- gamma/delta T cells. Neither CD40L surface expression nor CD40L mRNA were detected in unstimulated gamma/delta T cells. Stimulation with ...http://jem.rupress.org/content/181/3/1239
Gamma delta T cell - Wikipedia
Gamma delta T cells (γδ T cells) are T cells that have a distinctive T-cell receptor (TCR) on their surface. Most T cells are αβ (alpha beta) T cells with TCR composed of two glycoprotein chains called α (alpha) and β (beta) TCR chains. In contrast, gamma delta (γδ) T cells have a TCR that is made up of one γ (gamma) chain and one δ (delta) chain. This group of T cells is usually much less common than αβ T cells, but are at their highest abundance in the gut mucosa, within a population of lymphocytes known as intraepithelial lymphocytes (IELs). The antigenic molecules that activate gamma delta T cells are still largely unknown. However, γδ T cells are ...https://en.wikipedia.org/wiki/Gamma_delta_T_cell
TCR PAN γ/δ-FITC - Beckman Coulter
The TCR is a molecular complexe wich comprises two units: a recognition unit, composed of either alpha-beta or gamma-delta heterodimer, which are present on the cell surface in a mutually exclusive manner, and, a transducing unit, the CD3 complex, common to alpha-beta and gamma-delta heterodimers, which triggers the T cell when the recognition unit is occupied by the antigen. The recognition unit recognizes foreign antigens and the diversity necessary for this function of recognition is generated by somatic recombination the TCR genes. There are four TCR gene loci (alpha, beta, gamma and delta). Each of them is composed of several V (variable) segments, coding for about 90 amino acids, very short D (diversity) segments (alpha and delta loci only), and short J (joining) segments (about 15 ...https://www.beckman.com/reagents/coulter-flow-cytometry/antibodies-and-kits/single-color-antibodies/B49175
Design, synthesis, and biological evaluation of novel aminobisphosphonates possessing an in vivo antitumor activity through a...
A small series of aminobisphosphonates (N-BPs) structurally related to zoledronic acid was synthesized with the aim of improving activity toward activation of human gammadelta T cells and in turn their in vivo antitumor activity. The absence of the 1-OH moiety, together with the position and the different basicity of the nitrogen, appears crucial for antitumor activity. In comparison to zoledronic acid, compound 6a shows a greater ability to activate gammadelta T cells expression (100 times more) and a proapoptotic effect that is better than zoledronic acid. The potent activation of gammadelta T cells, in addition to evidence of the in vivo antitumor activity of 6a, suggests it may be a new potential drug candidate for cancer treatment.https://www.ndorms.ox.ac.uk/publications/107998
"Regulation of gammadelta versus alphabeta T lymphocyte differentiation" by Heather J. Melichar, Kavitha Narayan et al.
alphabeta and gammadelta T cells originate from a common, multipotential precursor population in the thymus, but the molecular mechanisms regulating this lineage-fate decision are unknown. We have identified Sox13 as a gammadelta-specific gene in the immune system. Using Sox13 transgenic mice, we showed that this transcription factor promotes gammadelta T cell development while opposing alphabeta T cell differentiation. Conversely, mice deficient in Sox13 expression exhibited impaired development of gammadelta T cells but not alphabeta T cells. One mechanism of SOX13 function is the inhibition of signaling by the developmentally important Wnt/T cell factor (TCF) pathway. Our data thus reveal a dominant pathway regulating the developmental fate of these two lineages of T ...http://escholarship.umassmed.edu/gsbs_sp/849/
Influence of Chemotherapy on Gamma-delta T Cells in Patients With Epithelial Ovarian Cancer - Full Text View - ClinicalTrials...
Ovarian cancer is associated with a high mortality rate. Treatment of ovarian cancer is currently based on surgery and chemotherapy.. The first surgery is a radical surgical procedure aiming to achieve no residual disease. Patients who could not benefit from the first surgery may be offered neoadjuvant chemotherapy with a new surgical procedure after 3 or 6 chemotherapy cycles. Chemotherapy is currently based on Taxol and Carboplatin. Immunotherapy based on Vgamma9Vdelta2 T cells could provide a promising therapeutic strategy, however, the impact of chemotherapy on the expansion and functional abilities of Vgamma9Vdelta2 T cells has never been evaluated.. We want to study the effect of chemotherapy of ovarian cancer on Vgamma9Vdelta2 T cells rates in the peripheral blood of patients with ovarian cancer.. Functional abilities of the ...https://clinicaltrials.gov/ct2/show/NCT01606358?term=ovarian+cancer&rank=16
Natural killer (NK) cells and gamma delta T-cells differentially modulate Th17-driven disease in ankylosing spondylitis - The...
Preparation Of Antigen-Presenting Human Gamma-Delta T Cells And Use In Immunotherapy - Patent application
0003]γδ T cells are a distinct subset of CD3+ T cells featuring TCRs that are encoded by Vγ- and Vδ-gene segments (Morita et al., 2000; Carding and Egan, 2002). They are further divided according to their primary residence in blood or tissues, the protein chain composition of their VγVδ-TCRs and their antigen selectivity. In humans, Vδ1+-TCR chain expressing γδ T cells (Vδ1+ T cells) predominate in epithelial or epithelia-associated/mucosal tissues of the skin, airways, digestive and urogenital tracts, and several internal organs, and constitute a minor fraction (,20%) of γδ T cells in peripheral blood. The TCRs of Vδ1+ T cells recognize lipid antigens presented by MHC-related CD1 molecules. Further, Vδ1+ T cells respond to stress-associated proteins, including MHC-related molecules MICA and MICB, and heat-shock proteins. ...http://www.patentsencyclopedia.com/app/20090208517
The Scripps Research Institute - News and Views
Wendy Havran, who was promoted to associate professor with tenure in the Department of Immunology. Havran ( B.S., Duke University; Ph.D., University of Chicago) joined TSRI in 1991. Her laboratory studies a unique subset of T lymphocytes, called "gamma-delta" T cells. These reside in epithelial tissues, which cover most of the internal and external surfaces of the body, and express a tissue-specific, invariant antigen receptor. Gamma-delta T cells are involved in tissue repair as well as epithelial inflammatory diseases, such as asthma and ulcerative colitis ...http://www.scripps.edu/newsandviews/e_20030224/promo.html
gamma delta TCR Mouse anti-Human, PerCP-eFluor 710, Clone: B1.1, eBioscience™ 25 tests; PerCP-eFluor 710 gamma delta TCR Mouse...
gamma delta TCR Mouse anti-Human, PerCP-eFluor 710, Clone: B1.1, eBioscience™ 25 tests; PerCP-eFluor 710 gamma delta TCR Mouse anti-Human, PerCP-eFluor 710,...https://www.fishersci.co.uk/shop/products/gamma-delta-tcr-mouse-anti-human-percp-efluor-710-clone-b1-1-ebioscience/15509476
NIOSHTIC-2 Publications Search - 20031974 - The role of gamma delta T cells in airway epithelial injury and bronchial...
BACKGROUND: Acute exposure to chlorine (Cl2) gas causes epithelial injury and airway dysfunction. gammadelta T cells are present in the mucosal surface of the airways and may contribute to the injury/repair response of the epithelium. METHODS: C57Bl/6J (wild type) and TCR-delta-/- mice exposed to Cl2 (400 ppm) for 5 minutes underwent measurements of airway responses to i.v. methacholine (MCh) at 1https://www.cdc.gov/niosh/nioshtic-2/20031974.html
IL17A - Wikipedia
Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A, often referred to as IL-17, was originally discovered at transcriptional level by Rouvier et al. in 1993 from a rodent T-cell hybridoma, derived from the fusion of a mouse cytotoxic T cell clone and a rat T cell lymphoma. Human and mouse IL-17A were cloned a few years later by Yao and Kennedy. Lymphocytes including CD4+, CD8+, gamma-delta T (γδ-T), invariant NKT and innate lymphoid cells (ILCs) are primary sources of IL-17A. Non-T cells, such as neutrophils, have also been ...https://en.wikipedia.org/wiki/IL17A
ZBTB16 - Wikipedia
La proteína 16 con dedos de zinc y un dominio BTB (ZBTB16) es un factor de transcripción codificado en humanos por el gen ZBTB16. Este gen es miembro de la familia de proteínas con dedos de zinc tipo C2H2 Kruppel y codifica un factor de transcripción que contiene nueve dominios dedos de zinc tipo Kruppel en el extremo C-terminal. La proteína ZBTB16 se localiza en el núcleo celular y está implicada en la progresión del ciclo celular, interaccionando con histona deacetilasas. Determinadas reorganizaciones aberrantes del locus del gen que codifica esta proteína se han asociado con el desarrollo de leucemia promielocítica aguda (APL) y con ciertos papeles fisiológicos en células Natural Killer de ratón y en células T gamma-delta. Se han descrito diversas variantes transcripcionales de este gen en humanos. La proteína ZBTB16 ha demostrado ser capaz de interaccionar con: ZBTB32 Factor de crecimiento semejante a EGF de unión a heparina ...https://es.wikipedia.org/wiki/ZBTB16
Tissue-specific emergence of regulatory and intraepithelial T cells from a clonal T cell precursor | Science Immunology
Immune cells act as TSA screeners in the intestine-tolerating commensal microbes and food antigens but remaining vigilant against attack. However, it has remained unclear how T cell precursors differentiate into the different cell types required to perform this balancing act. Now, Bilate et al. report that the T cell receptor (TCR) itself does not limit T cell fate to a single identity. The authors found that cells expressing a single TCR derived from a peripheral regulatory T cell (Treg) developed into either Tregs or CD4+CD8αα+ intraepithelial lymphocytes (CD4IELs) in the gut. This differentiation depended on cues from both the microbiota and the surrounding environment. ...http://immunology.sciencemag.org/content/1/2/eaaf7471.editor-summary
Regulation of Langerhans Cell Migration by Invariant Gamma Delta T Cells - Peter Kim
Dermatitis is a common manifestation of lupus that can cause scarring and disfigurement. Lupus dermatitis is often difficult to treat and relatively little is k...http://grantome.com/grant/NIH/K08-AR062593-03
KAKEN - Research Projects | Identification of the ligands of gamma delta T cells (KAKENHI-PROJECT-04454286)
Principal Investigator：SHIMADA Shinji, Project Period (FY)：1992 - 1994, Research Category：Grant-in-Aid for General Scientific Research (B), Research Field：Dermatologyhttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04454286/
Epidermal T cells and wound healing.
Abstract The murine epidermis contains resident T cells that express a canonical gammadelta TCR. These cells arise from fetal thymic precursors and use a TCR that is restricted to ..https://www.omicsonline.org/references/epidermal-t-cells-and-wound-healing-419312.html
Cd69 monoclonal antibody, clone H1.2F3 (Biotin) - (MAB5743) - Products - Abnova
Hamster monoclonal antibody raised against native Cd69. Native purified Cd69 from Y245 mouse dendritic epidermal T cell line. (MAB5743) - Products - Abnovahttp://www.abnova.com/products/products_detail.asp?catalog_id=MAB5743
It is not yet clear which are the activation signals underpinning this large expansion of polyclonal γδ T cells in this process. Proliferation was dependent on CD137L, IL2, and IL21, but the involvement of TCR signaling has not been tested. Although it is hard to conceive that aAPCs express the full array of TCR ligands for all expanded γδ T cells, engagement of TCR is suggested by the low TCR expression on generated Vδ2pos T cells and by the importance of CD137L for the expansion despite the absence of CD137 expression by γδ T cells prior expansion. Fisher and colleagues who coated B1 anti-γδTCR antibody on aAPCs showed it did not have a major role in γδ T-cell expansion even if this led to a better representation of Vδ2neg subsets. Gamma delta T cells have been shown to express HLA-I inhibitory receptors (7), so the absence of HLA-I ...http://clincancerres.aacrjournals.org/content/20/22/5573
Gentaur Molecular :Accu \ Mouse TCR gamma delta, Clone GL 3, Hamster Mab anti , FITC \ ACL7201F-3
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Questioning Answers: Pregnancy antiepileptic use and offspring autistic traits
A blog post discussing research suggesting an association between maternal use of antiepileptics during pregnancy and offspring scores on developmental and behavioural measures pertinent to autismhttps://questioning-answers.blogspot.co.uk/2013/09/pregnancy-antiepileptic-use-offspring-autistic-behaviours.html