A tumor specific single chain antibody dependent gene expression system. Oncogene 1999;18:559-64. ... with single chain variable fragment (scFv) antibodies: potential advantages over antibodies expressed within the intracellular ... Worn A, Pluckthun A. Stability engineering of antibody single-chain Fv fragments. J Mol Biol 2001;305:989-1010. ... Therefore, we prepared a single-chain antibody against human p65 (RelA subunit of NF-κB), which recognizes a COOH-terminal ...http://clincancerres.aacrjournals.org/content/13/7/2178
Antibody targeting of ovarian cancer: recombinant single-chain fragments and the role of internalization | SpringerLink
... the Hmited penetration of bulk tumor masses by macromolecules, cross... ... Among the factors that have hindered development of antibody based therapy are the heterogeneity of tumor antigen expression, ... 1992) Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-2 receptor. ... 1993) Highly specific in vivo tumor targeting by monovalent and divalent forms of 741F8 sFv, an anti-c-erbB2 single-chain Fv ...https://link.springer.com/chapter/10.1007%2F978-1-4757-0136-4_34
Intracellular Antibodies as Tools to Study ErbB Receptor Tyrosine Kinases | Springer for Research & Development
Intracellular expression of antibodies provides a novel approach for the manipulation of biochemical processes in intact cells ... Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-z receptor. Cancer ... EGF receptor and p185….-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells expressing ... Intracellular expression of a single-chain antibody directed to the EGFR leads to growth inhibition of tumor cells. Oncogene ...https://rd.springer.com/chapter/10.1007/978-3-662-12119-1_4
KAKEN - Researchers | KURAMASU Atsuo (90302091)
Journal Article] Expression of anti-HVEM single chain antibody on tumor cells induces tumor-specific immunity with long-term ... Journal Article] Expression of B7-H3, a potential factor of tumor immune evasion in combination with the number of regulatory T ...https://nrid.nii.ac.jp/nrid/1000090302091/
Plant Viral Vectors for Delivery by Agrobacterium | SpringerLink
Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci ... Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco ... High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system. Biotechnol ... Efficient single tobamoviral vector-based bioproduction of broadly neutralizing anti-HIV-1 monoclonal antibody VRC01 in ...https://link.springer.com/chapter/10.1007%2F82_2013_352
Cancer-Specific Ligand-Receptor Interactions | SpringerLink
... which exploits the abundance of specific molecular epitopes on cancer cells, has been proposed as a strategy to enhance the ... Mapping tumor epitope space by direct selection of single-chain Fv antibody libraries on prostate cancer cells. Cancer Res 64: ... High affinity restricts the localization and tumor penetration of single-chain fv antibody molecules. Cancer Res 61:4750-4755 ... Rudnick SI, Adams GP (2009) Affinity and avidity in antibody-based tumor targeting. Cancer Biother Radiopharm 24:155-161PubMed ...https://link.springer.com/chapter/10.1007/978-1-4614-7876-8_18
Molecules | Free Full-Text | Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End...
... single-chain variable fragments (scFvs) have potential for use in several applications, such as for diagnosis and treatment of ... Due to their lower production cost compared with monoclonal antibodies, ... Multimerization behaviour of single chain Fv variants for the tumour-binding antibody B72.3. Protein Eng. 1994, 7, 1027-1033. [ ... Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End-Product (AGE), with Reduced Inter- ...http://www.mdpi.com/1420-3049/22/10/1695/htm
Frontiers | DNA damage response and evasion from immunosurveillance in CLL: new options for NK cell-based immunotherapies |...
2005). MHC class I-related chain A conjugated to antitumor antibodies can sensitize tumor cells to specific lysis by natural ... 2010). Novel conjugates of single-chain Fv antibody fragments specific for stem cell antigen CD123 mediate potent death of ... the immunoligands utilize natural ligands for immune receptors fused to tumor-specific single chain variable fragment (scFv) or ... tumor-targeting antibodies enhance NKG2D-mediated NK cell cytotoxicity by stabilizing NK cell-tumor cell interactions. J. ...https://www.frontiersin.org/articles/10.3389/fgene.2015.00011/full
IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML
... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ... Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells ... Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other ... Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any ...http://www.mdpi.com/1422-0067/13/5/5420/htm
Single-chain antibody-based immunotoxins targeting Her2/neu: design optimization and impact of affinity on antitumor efficacy...
The MH3-B1/rGel construct with intermediate affinity showed effective tumor growth inhibition without inducing hepatotoxicity ... Constructs composed of intermediate-affinity antibodies are also potent agents that are more resistant to immune complex ... Against Her2/neu-overexpressing tumor cells, immunotoxins with increasing affinity displayed improved internalization and ... consisting of single-chain variable fragments (scFv) genetically fused to polypeptide toxins, represent potentially effective ...https://www.semanticscholar.org/paper/Single-chain-antibody-based-immunotoxins-targeting-Cao-Marks/5576983476a8c8f192c98d368fc236b9bde172c0
Antibodies | Free Full-Text | In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments
ScFvs have found applications as tools for in vivo loss-of-function studies and inactivation of specific protein domains, ... diagnostic imaging, tumor therapy and treatment for neurodegenerative and infectious diseases. This review will focus on their ... owing to better pharmacokinetic properties compared to the parent monoclonal antibodies and the relative ease of producing them ... expressed in vivo through viral vectors in instances where large infection rates and sustenance of high levels of the antibody ...http://www.mdpi.com/2073-4468/2/2/193
Combinatorial antigen recognition with balanced signaling promotes selective tumor eradication by engineered T cells. - PubMed...
a) Three different single chain variable fragments (scFvs) specific for PSCA were constructed and assembled into bispecific ... The bispecific antibodies were added in the indicated amounts to cocultures of T cells and 51Cr-labeled PSCA+ PC3 tumor cells ( ... Here we present a strategy to render T cells specific for a tumor in the absence of a truly tumor-restricted antigen. T cells ... Graphs, tumors were quantitatively measured using calipers and tumor volumes were plotted versus time for each tumor. Error ...https://www.ncbi.nlm.nih.gov/pubmed/23242161
Construction and Characterization of Bispecific Costimulatory Molecules Containing a Minimized CD86 (B7-2) Domain and Single...
... genetically fused to single-chain (sc) Fv antibody domains specific for the tumor-associated antigens epidermal growth factor ... EGF receptor and p185erbB-2-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells ... in a single polypeptide chain the costimulatory IgV-like domain of human CD86 and scFv antibody domains for specific tumor ... Domain and Single-Chain Antibody Fragments for Tumor Targeting. Florian Rohrbach, Bernhard Gerstmayer, Markus Biburger and ...http://clincancerres.aacrjournals.org/content/6/11/4314
Production and characterization of a human single-chain Fv to collagenase IV | SpringerLink
The over-expression of collagenase IV in tumor tissues was found to be closely related to tumor metastasis. Collagenase IV has ... eollagenase IV human single-chain antibody tumor metastasis Project supported by the National Natural Science Foundation of ... Based on phage display antibody library technique, a single-chain Fv specific for colbgenase IV was successfully cloned. This ... The affinity of hCo4 was found to be the same as that of a single-chain antibody constructed from a monoclonal antibody to ...https://link.springer.com/article/10.1007/BF02882738
Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-2 receptor. ... EGF receptor and p185erbB-2 specific single-chain antibody-toxins differ in their cell killing activity on tumor cells ... A bivalent single chain antibody-toxin specific for ErbB-2 and the EGF receptor.. Int. J. Cancer 65: 538-546, 1996.. PubMed ... Regression of cutaneous tumor lesions in patients intratumorally injected with a recombinant single-chain antibody-toxin ...http://www.georg-speyer-haus.de/de/forschung/forschungsgruppen/wels/publikationen.html
Fine Mutational Analysis of 2B8 and 3H7 Tag Epitopes with Corresponding Specific Monoclonal Antibodies | Korea Science
2B8 monoclonal antibody;3H7 monoclonal antibody;DrBphP protein;epitope mapping;tag; ... Fine Mutational Analysis of 2B8 and 3H7 Tag Epitopes with Corresponding Specific Monoclonal Antibodies - ... Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct. Biochim ... Their specific epitopes were identified in our previous report. We present here fine epitope mapping of these two antibodies by ...http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1BJB7_2016_v39n6_460
Single-chain variable fragment - Wikipedia
... drugs could be dosed much lower than other therapeutic antibodies and are capable of highly specific targeting of tumors in ... "Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue ... A single-chain variable fragment (scFv) is not actually a fragment of an antibody, but instead is a fusion protein of the ... Single-chain variable fragments lack the constant Fc region found in complete antibody molecules, and, thus, the common binding ...https://en.wikipedia.org/wiki/Single-chain_variable_fragment
The FGF-1-specific single-chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis，JOURNAL OF ... A novel single-chain variable fragment antibody against FGF-1 inhibits the growth of breast carcinoma cells by blocking the ... Hydrophobic poly (amino acid)-modified PEI-mediated delivery of single-chain antibody scFv1C9 inhibits HepG2 cell cycle process ... Preparation of Polyclonal Antibodies to Rubisco Large and Small Subunits and Their Application in the Functional Analysis of ...http://js.nenu.edu.cn/teacher/index.php?zgh=1995900005
Targeted drug delivery for cancer therapy: the other side of antibodies | Journal of Hematology & Oncology | Full Text
... tumor penetration, product heterogeneity and manufacturing issues. In view of the recognized importance of targeted drug ... In parallel to naked TMAs, antibody-drug conjugates (ADCs) have been developed for targeted delivery of potent anti-cancer ... Initial development efforts focused mainly on humanizing the antibody protein to overcome problems of immunogenicity and on ... Therapeutic monoclonal antibody (TMA) based therapies for cancer have advanced significantly over the past two decades both in ...https://jhoonline.biomedcentral.com/articles/10.1186/1756-8722-5-70
Enhanced immuno-viral therapy for solid tumors | BioPharma Dealmakers
The company is entering phase 1 trials for solid tumors with its lead product and is looking to partner with companies ... Icell Kealex Therapeutics is developing oncolytic vaccinia viruses armed with T-cell-engaging antibody fragments that boost the ... IKT's VVs secrete a so-called T cell engager consisting of two single-chain variable antibody fragments: a tumor-specific ... The tumor-specific fragment leads the T cell engagers to home to particular tumor cells, while the CD3-specific fragment binds ...https://biopharmadealmakers.nature.com/posts/15301-enhanced-immuno-viral-therapy-for-solid-tumors
James Swartz | Stanford Medicine Profiles
Antibody fragment scFvs contained a single exposed azide group using a scheme for site-specific incorporation of tyrosine ... A DNA vaccine encoding for an idiotype antibody single chain Fv (scFv) fragment fused to the Tetanus Toxin Fragment C (TTFrC) ... Consequently, the αCD19-Id molecule induced a robust Id-specific antibody response and protected animals from tumor challenge. ... We demonstrated that a fusion protein consisting of an idiotypic single chain Fv antibody fragment (scFv) linked to a cytokine ...https://med.stanford.edu/profiles/james-swartz
Shoshana Levy | Stanford Medicine Profiles
Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco ... The antibody immunoprecipitated a single chain protein of 26 kDa from cell lysates made with Triton X-100 but additional ... a subpopulation of tumor cells remained that had lost the tumor-specific heavy chain idiotypic determinant. Nucleotide sequence ... All antibodies reacting with the H chain or the intact IgM protein were idiotypically specific for 38C13. These monoclonal ...https://med.stanford.edu/profiles/shoshana-levy
The Targeted Immunocytokine L19-IL2 Efficiently Inhibits the Growth of Orthotopic Pancreatic Cancer | Clinical Cancer Research
... consisting of the human single-chain Fv antibody L19, which is highly specific for the extradomain B (ED-B) of fibronectin, and ... The highly specific antibody-mediated delivery of therapeutic agents to the tumor microenvironment might overcome this problem ... a human single-chain Fv (scFv) antibody fragment L19 has been generated, which displays picomolar binding affinity for ED-B, ... The most important prerequisite for a novel antibody-targeted delivery therapy like L19-IL-2 is the tumor-specific expression ...http://clincancerres.aacrjournals.org/content/14/15/4951.long
View of MODERN IMMUNOTHERAPY OF ADULT B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA WITH MONOCLONAL ANTIBODIES AND CHIMERIC ANTIGEN...
A CAR consists of a single chain variable antibody fragment highly specific to a tumor antigen, which is fused to the ... Blinatumomab is the first member of the novel class of BiTE® antibodies. It is a bispecific single-chain antibody construct ... naked antibodies, T-cell engaging bispecific single-chain (BiTE®) antibodies, and immunoconjugates/immunotoxins. The several ... BLAST: a confirmatory, single arm, phase 2 study of Blinatumomab, a bispecific T-cell engager (BiTE) antibody constructor, in ...https://www.mjhid.org/index.php/mjhid/article/view/2015.001/2367
Kim Janda - Wikipedia
"A Human Single-Chain Antibody Specific for Integrin α3β1 Capable of Cell Internalization and Delivery of Antitumor Agents". ... "De novo identification of tumor-specific internalizing human antibody-receptor pairs by phage-display methods". Journal of ... "Phage-Display Selection of a Human Single-Chain Fv Antibody Highly Specific for Melanoma and Breast Cancer Cells Using a ... prepared cell-surface glycosphingolipid can be utilized as a panning reagent to identify fully human single chain antibodies ( ...https://en.wikipedia.org/wiki/Kim_Janda