A tumor specific single chain antibody dependent gene expression system. Oncogene 1999;18:559-64. ... with single chain variable fragment (scFv) antibodies: potential advantages over antibodies expressed within the intracellular ... Worn A, Pluckthun A. Stability engineering of antibody single-chain Fv fragments. J Mol Biol 2001;305:989-1010. ... Therefore, we prepared a single-chain antibody against human p65 (RelA subunit of NF-κB), which recognizes a COOH-terminal ...http://clincancerres.aacrjournals.org/content/13/7/2178
Intracellular Antibodies as Tools to Study ErbB Receptor Tyrosine Kinases | Springer for Research & Development
Intracellular expression of antibodies provides a novel approach for the manipulation of biochemical processes in intact cells ... Selective inhibition of tumor cell growth by a recombinant single-chain antibody-toxin specific for the erbB-z receptor. Cancer ... EGF receptor and p185….-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells expressing ... Intracellular expression of a single-chain antibody directed to the EGFR leads to growth inhibition of tumor cells. Oncogene ...https://rd.springer.com/chapter/10.1007/978-3-662-12119-1_4
Cancer-Specific Ligand-Receptor Interactions | SpringerLink
... which exploits the abundance of specific molecular epitopes on cancer cells, has been proposed as a strategy to enhance the ... Mapping tumor epitope space by direct selection of single-chain Fv antibody libraries on prostate cancer cells. Cancer Res 64: ... High affinity restricts the localization and tumor penetration of single-chain fv antibody molecules. Cancer Res 61:4750-4755 ... Rudnick SI, Adams GP (2009) Affinity and avidity in antibody-based tumor targeting. Cancer Biother Radiopharm 24:155-161PubMed ...https://link.springer.com/chapter/10.1007/978-1-4614-7876-8_18
Molecules | Free Full-Text | Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End...
... single-chain variable fragments (scFvs) have potential for use in several applications, such as for diagnosis and treatment of ... Due to their lower production cost compared with monoclonal antibodies, ... Multimerization behaviour of single chain Fv variants for the tumour-binding antibody B72.3. Protein Eng. 1994, 7, 1027-1033. [ ... Production of Single-Chain Fv Antibodies Specific for GA-Pyridine, an Advanced Glycation End-Product (AGE), with Reduced Inter- ...http://www.mdpi.com/1420-3049/22/10/1695/htm
Frontiers | DNA damage response and evasion from immunosurveillance in CLL: new options for NK cell-based immunotherapies |...
2005). MHC class I-related chain A conjugated to antitumor antibodies can sensitize tumor cells to specific lysis by natural ... 2010). Novel conjugates of single-chain Fv antibody fragments specific for stem cell antigen CD123 mediate potent death of ... the immunoligands utilize natural ligands for immune receptors fused to tumor-specific single chain variable fragment (scFv) or ... tumor-targeting antibodies enhance NKG2D-mediated NK cell cytotoxicity by stabilizing NK cell-tumor cell interactions. J. ...https://www.frontiersin.org/articles/10.3389/fgene.2015.00011/full
IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML
... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ... Many phage display derived binders targeting important tumor markers have been identified. Selection directed to tumoral cells ... Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other ... Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any ...http://www.mdpi.com/1422-0067/13/5/5420/htm
Antibodies | Free Full-Text | In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments
ScFvs have found applications as tools for in vivo loss-of-function studies and inactivation of specific protein domains, ... diagnostic imaging, tumor therapy and treatment for neurodegenerative and infectious diseases. This review will focus on their ... owing to better pharmacokinetic properties compared to the parent monoclonal antibodies and the relative ease of producing them ... expressed in vivo through viral vectors in instances where large infection rates and sustenance of high levels of the antibody ...http://www.mdpi.com/2073-4468/2/2/193
Construction and Characterization of Bispecific Costimulatory Molecules Containing a Minimized CD86 (B7-2) Domain and Single...
... genetically fused to single-chain (sc) Fv antibody domains specific for the tumor-associated antigens epidermal growth factor ... EGF receptor and p185erbB-2-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells ... in a single polypeptide chain the costimulatory IgV-like domain of human CD86 and scFv antibody domains for specific tumor ... Domain and Single-Chain Antibody Fragments for Tumor Targeting. Florian Rohrbach, Bernhard Gerstmayer, Markus Biburger and ...http://clincancerres.aacrjournals.org/content/6/11/4314
Single-chain variable fragment - Wikipedia
... drugs could be dosed much lower than other therapeutic antibodies and are capable of highly specific targeting of tumors in ... "Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue ... A single-chain variable fragment (scFv) is not actually a fragment of an antibody, but instead is a fusion protein of the ... Single-chain variable fragments lack the constant Fc region found in complete antibody molecules, and, thus, the common binding ...https://en.wikipedia.org/wiki/Single-chain_variable_fragment
The FGF-1-specific single-chain antibody scFv1C9 effectively inhibits breast cancer tumour growth and metastasis，JOURNAL OF ... A novel single-chain variable fragment antibody against FGF-1 inhibits the growth of breast carcinoma cells by blocking the ... Hydrophobic poly (amino acid)-modified PEI-mediated delivery of single-chain antibody scFv1C9 inhibits HepG2 cell cycle process ... Preparation of Polyclonal Antibodies to Rubisco Large and Small Subunits and Their Application in the Functional Analysis of ...http://js.nenu.edu.cn/teacher/index.php?zgh=1995900005
Enhanced immuno-viral therapy for solid tumors | BioPharma Dealmakers
The company is entering phase 1 trials for solid tumors with its lead product and is looking to partner with companies ... Icell Kealex Therapeutics is developing oncolytic vaccinia viruses armed with T-cell-engaging antibody fragments that boost the ... IKT's VVs secrete a so-called T cell engager consisting of two single-chain variable antibody fragments: a tumor-specific ... The tumor-specific fragment leads the T cell engagers to home to particular tumor cells, while the CD3-specific fragment binds ...https://biopharmadealmakers.nature.com/posts/15301-enhanced-immuno-viral-therapy-for-solid-tumors
James Swartz | Stanford Medicine Profiles
Antibody fragment scFvs contained a single exposed azide group using a scheme for site-specific incorporation of tyrosine ... A DNA vaccine encoding for an idiotype antibody single chain Fv (scFv) fragment fused to the Tetanus Toxin Fragment C (TTFrC) ... Consequently, the αCD19-Id molecule induced a robust Id-specific antibody response and protected animals from tumor challenge. ... We demonstrated that a fusion protein consisting of an idiotypic single chain Fv antibody fragment (scFv) linked to a cytokine ...https://med.stanford.edu/profiles/james-swartz
Harnessing programmed cell death as a therapeutic strategy in rheumatic diseases
A fusion protein containing FasL and a fibroblast activation protein (FAP)-specific single chain antibody limited the induction ... Focusing FasL on tumor cells through a fusion protein combining FasL with a high-affinity antibody against the T-cell leukemia- ... Rituximab, a chimeric anti-CD20 B cell-specific monoclonal antibody that was originally developed to treat B cell malignancies ... Critical role for tumor necrosis factor-related apoptosis-inducing ligand in immune surveillance against tumor development. J ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3437254/?lang=en-ca
Kim Janda - Wikipedia
"A Human Single-Chain Antibody Specific for Integrin α3β1 Capable of Cell Internalization and Delivery of Antitumor Agents". ... "De novo identification of tumor-specific internalizing human antibody-receptor pairs by phage-display methods". Journal of ... "Phage-Display Selection of a Human Single-Chain Fv Antibody Highly Specific for Melanoma and Breast Cancer Cells Using a ... prepared cell-surface glycosphingolipid can be utilized as a panning reagent to identify fully human single chain antibodies ( ...https://en.wikipedia.org/wiki/Kim_Janda
Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for Cancer-Targeted Therapy - Reports of...
Blocking of CTLA-4 inhibits the growth of malignant tumor cells. Anti-CTLA-4 monoclonal antibodies activate the immune system ... Due to several advantages of single-chain antibodies (scFvs) compared ... ... Hosseinzadeh F, Mohammadi S, Nejatollahi F. Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for ... Production and Evaluation of Specific Single-Chain Antibodies against CTLA-4 for Cancer-Targeted Therapy ...http://rbmb.net/article-1-133-en.html
Molecular Mri for Sensitive and Specific Detection of Lung Metastases - Toxicologic Pathology
Early and specific detection of metastatic cancer cells in the lung (the most common organ targeted by metastases) could ... 2009) Single chain epidermal growth factor receptor antibody conju-gated nanoparticles for in vivo tumor targeting and imaging ... 2007) Specific targeting of tumor angiogenesis by RGD-conjugated ultrasmall superparamagnetic iron oxide particles using a ... Molecular Mri for Sensitive and Specific Detection of Lung Metastases. Home/Toxicologic Pathology Articles/Molecular Mri for ...https://focusontoxpath.com/molecular-mri-for-sensitive-and-specific-detection-of-lung-metastases/
IJMS | Free Full-Text | Bispecific Antibodies as a Development Platform for New Concepts and Treatment Strategies | HTML
The single-chain-based bispecific antibodies usually bridge tumor cells with immune cells and form an immunological synapse ... Bispecific antibodies in the IgG format include asymmetric bispecific antibodies and homodimerized bispecific antibodies, all ... Bispecific antibodies can also be used to treat hemophilia A by mimicking the function of factor VIII. Bispecific antibodies ... Bispecific antibodies that can simultaneously bind to cell surface antigens and payloads are a very ideal delivery system for ...http://www.mdpi.com/1422-0067/18/1/48/htm
Hetero-dimerization-activated Antibodies - James Larrick
The targets of most therapeutic antibodies are found on healthy cells which limits the development of truly tumor-specific ... Each demibody consists of 3 molecular components: (1) a single-chain variable fragment (scFv) to target a specific tumor cell- ... The targets of most therapeutic antibodies are found on healthy cells which limits the development of truly tumor-specific ... unlike conventional bispecific antibodies, our antibody fragments dimerize at the tumor cell surface ONLY IF both targets are ...http://grantome.com/grant/NIH/R43-AI098320-01
Publikationen mit Erscheinungsjahr 1995
²-specific single-chain antibody toxins differ in their cell-killing activity on tumor cells expressing both receptor proteins. ... Site-specific and doping-dependent electronic structure of YBa₂Cu₃Oₓ probed by O 1s and Cu2p X-ray absorption spectroscopy. ... Site-specific and doping-dependent electronic structure of YBa₂Cu₃Oₓ probed by O 1s and Cu2p X-ray-absorption spectroscopy. ... A single amino acid exchange abolishes dimerization of the androgen receptor and causes Reifenstein syndrome.. Gast, A.; ...https://publikationen.bibliothek.kit.edu/index.php?year=1995&hitsperpage=1000&page=7
Cancer Immunotherapy: New Technology to Target Cancer Cells | MedCrave
... and bind to the target cells via an antibody derived single chain, which is specific for a tumour-associated antigen [15,16]. ... This evasion of tumor cells from immunosurveillance is reported in 19% to 92% of tumor cells and often associated with poor ... 2014) The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor ... Sznol M, Chen L (2013) Antagonist antibodies to PD-1 and B7-H1 (PD-L1) in the treatment of advanced human cancer. Clin Cancer ...http://googlescholar.medcraveonline.com/scholars/article_fulltext/4793
Nanobody-Based Targeting of the Macrophage Mannose Receptor for Effective In Vivo Imaging of Tumor-Associated Macrophages |...
... which are single-domain antigen-binding fragments derived from Camelidae heavy-chain antibodies. MMR-specific nanobodies ... Selection and identification of single domain antibody fragments from camel heavy-chain antibodies. FEBS Lett 1997;414:521-6. ... Antibody-based tumor-targeting strategies are widely explored (9, 10). Antibodies can be used for tumor imaging or delivering ... Efficient tumor targeting by single-domain antibody fragments of camels. Int J Cancer 2002;98:456-62. ...http://cancerres.aacrjournals.org/content/72/16/4165
CONSTITUTIVE EXPRESSION OF COSTIMULATORY LIGANDS ON ADOPTIVELY TRANSFERRED T LYMPHOCYTES - Patent application
... receptor specific for the tumor antigen PSMA. The Pz1 receptor comprises a PSMA-binding single-chain antibody fragment fused to ... 0088] Tumor antigen-specific T lymphocytes (and NK cells)  Types of tumor antigen-specific human lymphocytes that can be ... non-HLA-restricted antigen receptor specific for the tumor antigen PSMA. The Pz1 receptor comprises a PSMA-binding single chain ... measurable tumor. A clinically measurable tumor is one that can be detected on the basis of tumor mass (e.g., by palpation, CAT ...http://www.patentsencyclopedia.com/app/20130121960
Anti-pan Cadherin antibody (Alexa Fluor® 647) [EPR1792Y]
Anti-pan Cadherin recombinant antibody conjugated to Alexa Fluor® 647 [EPR1792Y] validated for ICC/IF and tested in Human, ... Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell ... Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual ... Cadherins play an important part in tumor invasion and metastasis.. * Database links ...http://www.abcam.com/pan-cadherin-antibody-epr1792y-intercellular-junction-marker-alexa-fluor-647-ab195203.html
Anti-pan Cadherin antibody (ab74635) | Abcam
Rabbit polyclonal pan Cadherin antibody validated for IHC and tested in Human, Mouse, Rat, Chk, Cow, Dog, Xl and Mk. Immunogen ... Cadherins are members of a multigene family of single chain glycoprotein receptors mediating calcium dependent cell-cell ... Cadherins are expressed in a tissue specific manner and and are required for assembly of cells into solid tissue. Individual ... Cadherins play an important part in tumor invasion and metastasis.. * Database links ...http://www.abcam.com/pan-cadherin-antibody-ab74635.html
The Antibody-Based Delivery of Interleukin-12 to the Tumor Neovasculature Eradicates Murine Models of Cancer in Combination...
... immunocytokine based on the sequential fusion of interleukin-12 as a single polypeptide with two F8 antibodies in single-chain ... 26, 27), an antibody fragment specific to the alternatively spliced EDB domain of fibronectin (28). However, the tumor ... The gene structure for the F8 antibody in diabody format (36) and the isolation of the KSF antibody, specific to hen egg ... Data represent mean tumor volumes ± SE. Tumor growth curves were stopped when the first tumor per group reached 2,000 mm3. D, ...http://clincancerres.aacrjournals.org/content/18/15/4092
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