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*  Polyclonal Antibodies - Peptides, Peptide Synthesis, Peptide Library, Custom Peptides
Anti-phosphopeptide Antibodies * Literature * Combinatorial Chemistry * Introduction to SynPhase Lanterns * Understanding ... The detector antibody can either be an affinity purified biotinylated rabbit anti peptide antibody or as illustrated, an anti- ... The illustration opposite shows the primary capture antibody as an affinity purified rabbit antipeptide antibody. The captured ... Good anti-peptide antibodies make great reagents for the rapid development of immunoassays. Mimotopes has extensive experience ...
  http://www.mimotopes.com/knowledgeBaseDocument.asp?did=67&cid=24,71
*  Early detection of diabetes - The Johns Hopkins University
The methods will be based upon the successful schemes developed for phosphopeptide antibodies. ... 9. The method of claim 8, wherein the antibody is a monoclonal antibody. 10. The method of claim 8, wherein the antibody is ... As such, the term "O-GlcNAc antibody" refers to an antibody that binds to O-GlcNAc. Similarly, the term "naked site antibody" ... wherein the second antibody is a monoclonal antibody. 4. The method of claim 1, wherein the second antibody is detectably ...
  http://www.freepatentsonline.com/9250248.html
*  Protocols and Video Articles Authored by Wayne S. Sossin (Translated to Hebrew)
Phosphopeptide-specific Antibodies to Protein Kinase C Methods in Molecular Biology (Clifton, N.J.). 2003 , Pubmed ID: 12840511 ... We monitored the PDK-dependent phosphorylation of PKC Apl I and PKC Apl II using phosphopeptide antibodies. During persistent ...
  https://www.jove.com/author/Wayne+S._Sossin?language=Hebrew
*  Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. -...
80% of the peptide mixture was subjected to anti-phosphotyrosine antibody-based phosphopeptide enrichment. The residual 20% of ... The enriched phosphopeptides from both enrichment methods were analyzed by LC-MS/MS. The residual 10% of SCX-fractionated ... The 90% of the SCX-fractionated peptides were merged and subjected to TiO2-based phosphopeptide enrichment. ...
  https://www.ncbi.nlm.nih.gov/pubmed/25263469
*  Phosphorylation of eucaryotic translation initiation factor 4B Ser422 is modulated by S6 kinases | The EMBO Journal
Phospho‐eIF4B(Ser422) polyclonal antibodies were produced by immunizing rabbits with a synthetic KLH‐coupled phosphopeptide ( ... Analysis using site‐specific anti‐phosphopeptide antibodies. J Biol Chem 273: 16621-16629. ... S6K1 phosphorylates eIF4B in vitro on a phosphopeptide comigrating with the endogenous serum‐stimulated phosphopeptide 2. (A) ... sensitive phosphopeptide 2, strongly suggesting that the phosphopeptide generated in vitro is identical to that generated in ...
  http://emboj.embopress.org/content/23/8/1761
*  Phosphodiesterase 4D acts downstream of Neuropilin to control Hedgehog signal transduction and the growth of medulloblastoma |...
The second measure of PKA activity is the use of a PKA consensus phosphopeptide antibody on Western blots. This has been used ... The panA antibodies might seem to serve the same purpose but antibody binding could have additional consequences: blocking ... The second measure of PKA activity is the use of a PKA consensus phosphopeptide antibody on Western blots. This has been used ... The antibody NrppanA occupies Sema3's binding site in both Nrp1 and Nrp2; antibodies Nrp1B and Nrp2B occupy VEGF's binding ...
  https://elifesciences.org/articles/07068
*  Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase |...
Polyclonal antibodies were evaluated after affinity purification on phospho-peptide antigen column, and for rabbit antibodies, ... with total Rab35 antibody, 10% with Rab8A antibody and 10% with total Rab10 antibody (all at 1 µg/ml antibody). The ... Polyclonal phospho-specific Rab protein antibodies. We first raised sheep polyclonal antibodies against phospho-peptides ... of 0.2 µg of antibody per 1 µl of beads for monoclonal antibody or 1 µg of antibody per 1 µl of beads for polyclonal antibody. ...
  http://www.biochemj.org/content/475/1/1
*  Purification Enrichment | Sigma-Aldrich
The PhosphoProfile I Phosphopeptide Enrichment Kit is designed to increase the concentration of phosphopeptides from tryptic ... The enriched solution helps to overcome the limitations of mass spectrometry of phosphopeptides. The provided reagents are ... affinity enrichment of phosphopeptides obtained from protein tryptic digests and direct transfer of phosphocompounds for ... Phosphotyrosine-BSA Phosphotyrosine Antibody Inhibitor, 2 mg per ml, Buffered aqueous solution, for blotting and ELISA. P3967. ...
  https://www.sigmaaldrich.com/life-science/proteomics/post-translational-analysis/phosphorylation/purification-enrichment.html
*  Peptide microarray - Wikipedia
"Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence- ... by a primary antibody that targets the bound protein or modified substrates. After several washing steps a secondary antibody ... Usually, the secondary antibody is tagged by a fluorescence label that can be detected by a fluorescence scanner. Other label- ... This peptide chip can directly be incubated with a variety of different biological samples like purified enzymes or antibodies ...
  https://en.wikipedia.org/wiki/Peptide_microarray
*  Phospho anti-Mylc2b (S18) antibody (ab63479) | Abcam
... antibody validated for ELISA, IHC and tested in Human. Referenced in 1 publication. Immunogen corresponding to synthetic… ... The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the ... Primary antibodies. Secondary antibodies. ELISA, Matched Antibody Pairs and Multiplex Immunoassays. Cell and tissue imaging ... Synthetic phosphopeptide (Human) derived from Mylc2b from around the phosphorylation site of serine 18 (A-T-SP-N-V). ...
  https://www.abcam.com/mylc2b-phospho-s18-antibody-ab63479.html
*  ProbesOnline™ July 2013-Add-and-image fluorescent antibody formulations, a kit for glucose quantitation, cytotoxicity assays |...
A New Antibody for Studying Cell Junctions-JAM-A ABfinity™ Recombinant Rabbit Oligoclonal Antibody , Ready-to-Use ... the lack of antibody signal in cells treated with both antibody and phosphopeptide demonstrates the antibody's specificity. ... recombinant antibodies-New antibody for Bcl-2 What they are. ABfinity™ recombinant monoclonal and oligoclonal antibodies offer ... Bcl-2 Oligoclonal Antibody, ABfinity™ Recombinant. 100 µg. 710515. Bcl-2 Monoclonal Antibody (clone Bcl-2-100), Mouse. 1 mL. ...
  http://www.thermofisher.com/ar/es/home/references/newsletters-and-journals/probesonline/probesonline-issues-2013/probesonline-jul-2013.html
*  anti-MTOR Antikörper (AA 2415-2464, pSer2448) | Produkt Nr. ABIN1531910
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non- ... Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using mTOR (Phospho-Ser2448) Antibody. The ... Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using mTOR (Phospho-Ser2448) Antibody. The ... Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', using mTOR (Phospho-Ser2448) Antibody. The ...
  http://www.antikoerper-online.de/antibody/1531910/anti-Mechanistic+Target+of+Rapamycin+serine/threonine+Kinase+FRAP1+pSer2448,+AA+2415-2464+antibody/
*  MYC (phospho T58) polyclonal antibody - (PAB25255) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding T58 of human MYC. (PAB25255) - Products - Abnova ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of MYC. ... Western blot analysis of extracts from human ovary cancer cells using MYC (phospho T58) polyclonal antibody (Cat # PAB25255). ... Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MYC (phospho T58) polyclonal antibody(Cat ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB25255
*  RELA (phospho T435) polyclonal antibody - (PAB25233) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding T435 of human RELA. (PAB25233) - Products - Abnova ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA. ... Western blot analysis of extracts from COS7 cells using RELA (phospho T435) polyclonal antibody (Cat # PAB25233). ... Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RELA (phospho T435) polyclonal antibody ( ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB25233
*  RELA (phospho T254) polyclonal antibody - (PAB8192) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding T254 of human RELA. (PAB8192) - Products - Abnova ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of RELA. ... Western blot analysis of extracts from 3T3 cells treated with TNF-alpha using RELA (phospho T254) polyclonal antibody (Cat # ... Immunofluorescence staining of methanol-fixed HeLa cells using RELA (phospho T254) polyclonal antibody (Cat # PAB8192). ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB8192
*  EphrinB (phospho Y317) polyclonal antibody - (PAB9611) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding Y317 of chicken EphrinB. (PAB9611) - Products - Abnova ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of EphrinB. ... polyclonal antibody (Cat # PAB9611). The immunolabeling of the EphrinB is completely eliminated by treatment with lambda-Ptase. ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB9611
*  PRKAA1 (phospho S79) polyclonal antibody - (PAB12747) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding S79 of Carassius auratus PRKAA1. (PAB12747) - Products - Abnova ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKAA1. ... This antibody recognizes PRKAA1 with a phosphorylated site of Ser 79. It does not cross-react with non-phosphospecific peptide. ... The whole cell lysate derived from serum starved HEK 293 was immunoblotted by Prkaa1 (phopho S79) polyclonal antibody (Cat # ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB12747
*  KIT (phospho Y719) polyclonal antibody - (PAB16884) - Products - Abnova
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y719 of KIT. (PAB16884) - Products - ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of KIT. ... Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y719 of KIT. ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB16884
*  AKT1 (phospho S473) polyclonal antibody - (PAB4918) - Products - Abnova
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1. (PAB4918) - Products ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1. ... 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL. ... Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1. ...
  https://www.abnova.com/products/products_detail.asp?Catalog_id=PAB4918
*  RPS6 (phospho S240/S244) monoclonal antibody, clone SP45 - (MAB6559) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding S240/S244 of human RPS6. (MAB6559) - Products - Abnova ... Rabbit monoclonal antibody raised against synthetic phosphopeptide of RPS6. ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=MAB6559
*  STAT4 (phospho Y693) polyclonal antibody - (PAB12755) - Products - Abnova
Synthetic phosphopeptide corresponding to residues surrounding Y693 of human, mouse, rat, bovine, chicken and dog STAT4. ( ... Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT4. ... This antibody recognizes STAT4 with a phosphorylated sites of Tyr693. It does not cross-react with non-phosphospecific peptide. ... Synthetic phosphopeptide corresponding to residues surrounding Y693 of human, mouse, rat, bovine, chicken and dog STAT4. ...
  http://www.abnova.com/products/products_detail.asp?catalog_id=PAB12755
*  Peptide-Based Microarray | Springer for Research & Development
Profiling of generic anti-phosphopeptide antibodies and kinases with peptide microarrays using radioactive and fluorescence- ... Lesaicherre, M.L., Uttamchandani, M., Chen, G.Y.J., and Yao, S.Q. (2002b) Antibody-based fluo rescence detection of kinase ... Yu, Z., Tu, J., and Chu, Y.-H. (1997) Confirmation of crossreactivity between lyme antibody H9724 and human heat shock protein ... Functional peptide microarrays for specific and sensitive antibody diagnostics. Proteomics, 6, 1376-1384.CrossRefGoogle Scholar ...
  https://rd.springer.com/chapter/10.1007/978-0-387-72719-6_7
*  Synuclein-alpha Antibody (Phospho-Tyr136) (OAAF07792) | Rabbit Polyclonal Antibody
Polyclonal Antibody , Application: WB, ELISA , Species Reactivity: Human, Mouse, Rat , Alias: Alpha-synuclein; Non-A beta ... The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non- ... SNCA antibody - middle region (ARP42350_P050). Application: WB. Species: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat ... Synuclein Antibody (Phospho-Ser129) (OAAF07443). Application: WB, IHC, ELISA. Species: Human, Mouse, Rat. ...
  https://www.avivasysbio.com/en/synuclein-alpha-antibody-phospho-tyr136-oaaf07792.html