Next message: collagen antibodies * Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] hi, netters! I need help on ... help on phosphopeptide purification. Klaus Weber kweber at vms.biochem.mpg.de Wed Dec 1 08:36:01 EST 1993 * ... of the phosphopeptide gets lost by adsorbing to the inner metal surface. Did you experience such problems and if so, how did ...

... phosphopeptides are enriched using phosphospecific antibodies, immobilized metal affinity chromatography or titanium dioxide ( ... Antiphosphotyrosine antibodies have been proven very successful in purification, but fewer reports have been published using ... To begin with, isolation methods such as anti-phosphotyrosine antibodies do not distinguish between isolating tyrosine- ... This is due to the optimization of enrichment protocols for phosphoproteins and phosphopeptides, better fractionation ...

... antibody. Validated in WB, ELISA, IHC, ICC/IF and tested in Mouse, Human. Cited in 9 publication(s). Independently reviewed in ... The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the ... Primary antibodies. Secondary antibodies. ELISA and Matched Antibody Pair Kits. Cell and tissue imaging tools. Cellular and ... The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. ...

Increased phosphorylation of LMNA and NIPA was confirmed using specific antibodies. To expand our verification capability, we ... In sample I used for discovery experiment, we successfully quantified 32 (in SIM mode) and 30 (in AIMS mode) phosphopeptides ... When the assay was applied to other three pairs of HCC specimens for verification experiment, 40 target phosphopeptides were ... Super-SILAC mix coupled with SIM/AIMS assays for targeted verification of phosphopeptides discovered in a large-scale ...

Selected hybridomas, producing antibodies that reacted specifically with the phosphopeptide, were cloned twice by limiting ... Commercial antibodies. Rabbit polyclonal antibodies were: PKD, phospho-PKD-S916 and phospho-PKD-S744/748, p38 and phospho-p38-T ... 2). This antibody was named: Phospho-PKD1 (Tyr93) monoclonal antibody (clone 6E8). Its isotype was determined by ELISA as IgG2b ... with primary antibodies overnight at 4 °C and subsequently incubated 2 h at 4 °C with secondary antibodies. Then, slices were ...

Phosphopeptide containing human EGF R Y1068 site Formulation. Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium ... Reviews for Human Phospho-EGFR/ErbB1 (Y1068) Alexa Fluor® 488-conjugated Antibody. There are currently no reviews for this ... Citation for Human Phospho-EGFR/ErbB1 (Y1068) Alexa Fluor® 488-conjugated Antibody. R&D Systems personnel manually curate a ... Have you used Human Phospho-EGFR/ErbB1 (Y1068) Alexa Fluor® 488-conjugated Antibody?. Submit a review and receive an Amazon ...

This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL purified antibody (1 mg/mL). ... KLH conjugated synthetic phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser393. View immunogen .st0 ... If an Invitrogen™ antibody doesnt perform as described on our website or datasheet,well replace the product at no cost to you ... Cite Phospho-PDPK1 (Ser393) Polyclonal Antibody. The following product was used in this experiment: Phospho-PDPK1 (Ser393) ...

The antiserum was produced against synthesized phosphopeptide derived from human IKK-? around the phosphorylation site of ... The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.. ... IKK-. (Phospho-Ser31) antibody detects endogenous levels of IKK-. only when phosphorylated at serine 31.. ... Get a free Anti-DDK antibody sample free with this product. Use code: "DDK-Lysate". View details » ...

Phospho-Y39 α-synuclein polyclonal antibody generation. The α-synuclein phosphopeptide TKEGVLY(p)VGSKTKEGC was conjugated to ... Antibodies. Rabbit polyclonal phosphotyrosine 39 α-synuclein antibodies were generated as described below. Primary antibodies ... Antibodies to α-synuclein coimmunoprecipitate c-Abl from WT and hA53Tα-syn but not c-AblKO nor c-AblKO hA53Tα-syn mice (Figure ... or anti-pY125 α-syn antibodies. Inputs were immunoblotted with anti-GFP antibodies. All experiments were repeated at least 3 ...

79-168) can be used to detect Human, Mouse, Rat AFX (phospho Ser197) Antibody in WB and other applications. ... Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were ... Custom Antibody Services Speak to one of our custom antibody specialists to discuss your next custom antibody project. ... Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.. Contact our Customer Care Team for more ...

This antibody reacts with Human, Rat samples. Supplied as 100 µL purified antibody. ... Synthetic phosphopeptide corresponding to residues surrounding pTyr231 of human CaMKII View immunogen .st0{fill:#FFFFFF;} .st1{ ... Phospho-CaMKII alpha/beta/delta (Tyr231) Polyclonal Antibody. View all (7) CaMKII alpha/beta/delta antibodies ... using Phospho-CaMKII pTyr231 polyclonal antibody (Product # PA5-17108) (upper) or CaMKII antibody (lower). {{ $ctrl. ...

Highly specific and rigorously validated in-house, Phospho-FLT3 (Tyr591) Antibody (CST #3461) is ready to ship. ... Polyclonal Antibody for studying FLT3 (Tyr591) phosphate. Cited in 36 publications. Validated for Western Blotting. ... Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding ... Primary Antibody Incubation. *Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in ...

Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. Conjugation Unconjugated. ... Left : Primary antibody. Right : Primary antibody pre-incubated with the antigen specific peptide ... There are currently no reviews for CaMKIV (phospho Thr200) antibody (GTX55402). Be the first to share your experience with this ... There are currently no references for CaMKIV (phospho Thr200) antibody (GTX55402). Be the first to share your publications with ...

... Validated for FACS. Tested in Human. Order online. ... KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Thr249 Isotype IgG ... JRA antibody, JUN antibody, Jun antibody, V antibody, cJun antibody, d-JRA antibody, d-Jun antibody, d-jun antibody, dAP-1 ... dJUN antibody, dJra antibody, dJun antibody, djun antibody, dm-Jun antibody, jra antibody, jun antibody, l(2)46Ef antibody, l(2 ...

Antibody, Catalog OAAB16210-400UL. Test in Dot blot applications. Reacts with Human, Porcine, Rat samples. ... 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.. ... This MEF2C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino ... Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein ...

Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non- ... Anti-GATA1 (phospho-Ser310) Antibody (43042) quantity. Add to cart. SKU: 43042 Categories: Antibody Products, Phospho-specific ... Specificity This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 310. ... Research Areas Phosphospecific Antibodies. Background GATA1 encodes a protein, GATA1, which belongs to the GATA family of ...

... a phosphopeptide is used as antigen to generate in rabbits 5-10 mg of affinity-purified antibody within 4 months. ... Polyclonal Antibody Production Services. We are proud of offering "Made in USA" quality label for these services by using our ... Abbiotec offers custom antibody production services to the scientific community to accelerate a project Biology to Discovery™. ... Every antibody project is supplied with a Peptide Design and Project reports, when applicable. Abbiotec guarantees that the ...

Analysis using site-specific anti-phosphopeptide antibodies. J Biol Chem 273:16621-16629. 6. Lipton JO, Sahin M(2014) The ...

Polyclonal pT170 antibodies were affinity-purified using the human PLK4 phosphopeptide coupled to a SulfoLink Coupling Resin ( ... Polyclonal pS428 antibodies were affinity-purified using the hSTIL phosphopeptide coupled to a SulfoLink Coupling Resin (Thermo ... Antibody production. Request a detailed protocol Human Centrin2 (a.a. 1-172) was cloned into a pET-23b bacterial expression ... Antibody incubations were conducted in the blocking solution for 1 hr. DNA was detected using DAPI, and cells were mounted in ...

ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) (pThr202/Tyr204) from human. It is ... Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH). ... Antibodies All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control ... Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies ...

The Anti-ETK (Phospho-Tyr566) antibody is highly specific for the phospho-peptide in comparison to the non- phospho peptide ... 100x Anti-ETK antibody: This antibody is a rabbit polyclonal antibody. This antibody was tested to be specific for the ETK ... 100x Anti-GAPDH antibody: This antibody is a mouse monoclonal antibody. This antibody was tested to be specific for GAPDH. The ... 11) Add 50 µL of 1x primary antibodies (Anti-ETK (Phospho-Tyr566) antibody, Anti-ETK antibody and/or Anti-GAPDH antibody) to ...

Phosphopeptide region of human Rb containing serine 612. Activity cross reaction. Reacts with human Retinoblastoma protein ( ... Store antibody at -80°C. Upon initial thawing, aliquot in appropriate aliquots and store at -80°C. Avoid multiple freeze-thaw ... Retinoblastoma protein (pSer612) antibody Retinoblastoma-associated protein. RB transcriptional corepressor 1. p105-Rb. pRb, Rb ...

... antibody. Validated in WB, IHC and tested in Human. Cited in 52 publication(s). Independently reviewed in 8 review(s). ... The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by ... Primary antibodies. Secondary antibodies. ELISA and Matched Antibody Pair Kits. Cell and tissue imaging tools. Cellular and ... All lanes : Anti-Histone H3 (phospho S10) antibody (ab47297). Lane 1 : EGF + Calyculin treated HeLa cells + phospho-peptide.. ...

Inactivated PEPS reacted with the anti-phosphopeptide antibodies while activated PEPS did not (Figure 3). However, it should be ... And finally, preincubation of the antibody with the synthetic peptide used to raise the antibodies eliminated any labeling of ... proteins separated and probed with anti-phosphothreonine phosphopeptide antibodies.. Figure 3. Time-course of the ADP/ATP- ... with the anti-phosphothreonine antibodies (Figure 2). The antibody labeling of PPDK in samples removed from an inactivation ...

The albumin and IgG in the sample, which were bound by antibodies immobilized on a solid support in the spin column, were mixed ... Again, several phosphopeptides were identified from both depletion fraction and albumin/IgG bound fraction from albumin/IgG ... Again, several phosphopeptides were identified from both depletion fraction and albumin/IgG bound fraction from albumin/IgG ... The sample was then mixed with 50 L Domatinostat tosylate of phosphotyrosine-specific antibody 4G-10 immobilized on agarose in. ...

Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide ... MBS8529139 , Phospho-RIPK3 (Ser316) Antibody MyBiosource Antibodies MBS8529139 , Phospho-RIPK3 (Ser316) Antibody. (No reviews ... MyBiosource Antibodies. MBS8529139 , Phospho-RIPK3 (Ser316) Antibody. Rating Required Select Rating. 1 star (worst). 2 stars. 3 ... Seed G17 antibody , MBS839338 , MyBiosourceProduct Short Name: [Seed G17]Product Name Synonyme: [Polyclonal Seed G17; Anti-Seed ...

Dashed lines explain how core phosphopeptides are defined. A Venn diagram of modified phosphopeptides in at least one condition ... Phospho-effector abundance of PI3K/Akt/mTOR pathway was assessed by WB analysis using indicated antibodies, in samples ... Dashed lines explain how core phosphopeptides are defined. A Venn diagram of modified phosphopeptides in at least one condition ... phosphopeptides (Fig. 2B and C; Supplementary Fig. S3). The finding that there is a common "core" of phosphopeptides regulated ...

... that includes ELISA reagents and 2 antibodies to monitor levels of both total ErbB-2 and phospho ErbB-2 (Y877) or (Y1248), ... The FACE ErbB-2 (Y877) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide ... HRP-conjugated secondary antibody, Quenching Solution, 1X Antibody Blocking Buffer, 1X Antibody Dilution Buffer, 10X PBS, 10% ... The FACE ErbB-2 (Y1248) Kit contains a phospho-ErbB-2 antibody that was raised in rabbit against a synthetic phospho-peptide ...

Antibodies, Phospho-Specific*Antibodies, Phospho-Specific. *Antibodies, Phospho Specific. *Antibodies, Phosphopeptide-Specific ... "Antibodies, Phospho-Specific" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ... This graph shows the total number of publications written about "Antibodies, Phospho-Specific" by people in this website by ... Antibodies directed against immunogen-coupled phosphorylated PEPTIDES corresponding to amino acids surrounding the ...

Selective binding of activated pp60c-src by an immobilized synthetic phosphopeptide modeled on the carboxyl terminus of pp60c- ... Selective binding of activated pp60c-src by an immobilized synthetic phosphopeptide modeled on the carboxyl terminus of pp60c- ...