*  IJMS | Free Full-Text | The Clinical Utilization of Circulating Cell Free DNA (CCFDNA) in Blood of Cancer Patients | HTML
Thus, point mutations, copy number variations, loss of heterozygosity or aneuploidy can be detected. Also, digital PCR can ... Loss of Heterozygosity (LOH). Loss of Heterozygosity (LOH) analysis of alleles at specific chromosomes of cell free plasma or ... Pang, J.Z.; Qin, L.X.; Wang, Q.Q.; Ren, N.; Sun, B.S.; Lin, G.L.; Ye, Q.H.; Liu, Y.K.; Tang, Z.Y. Loss of heterozygosity of ... Nakamura, T.; Sunami, E.; Nguyen, T.; Hoon, D.S. Analysis of loss of heterozygosity in circulating DNA. Methods Mol. Biol 2009 ...
  http://mdpi.com/1422-0067/14/9/18925/htm
*  Frontiers | Whole Genome-Based Amplified Fragment Length Polymorphism Analysis Reveals Genetic Diversity in Candida africana |...
... aneuploidy, and loss of heterozygosity. Cold Spring Harb. Perspect. Med. 4:a019604. doi: 10.1101/cshperspect.a019604 ... and loss of heterozygosity (LOH), that affect large genomic regions and shape the evolution of new strains (Bennett et al., ... in which phenotypic plasticity of the isolates appears to be driven mainly by LOH events as well as by chromosomal aneuploidies ...
  https://www.frontiersin.org/articles/10.3389/fmicb.2017.00556/full
*  Ploidy dynamics and evolvability | Philosophical Transactions of the Royal Society B: Biological Sciences
Aneuploidy and loss of heterozygosity (LOH), which are large-scale genome shifts involving whole chromosomes or chromosome arms ... These often result in a high level of aneuploidy and loss of heterozygosity (LOH) [3], which can lead to clinical consequences ... 3. Effects of ploidy shifts and aneuploidy on fitness. *4. Loss of heterozygosity as a long-term mechanism of rapid genome ... Antifungal drug exposure can induce aneuploidy and/or loss of heterozygosity. (a) Flow cytometry data comparing DNA content of ...
  http://rstb.royalsocietypublishing.org/content/371/1709/20150461
*  Human Fungal Pathogens
Rapid Mechanisms for Generating Genome Diversity: Whole Ploidy Shifts, Aneuploidy, and Loss of Heterozygosity. Richard J. ...
  https://www.cshlpress.com/default.tpl?action=full&cart=1510732518122069775&--eqskudatarq=1036
*  Cell-free fetal DNA - Wikipedia
Point mutations, loss of heterozygosity and aneuploidy can be detected in a single PCR step. Digital PCR can differentiate ... Allyse, M.; Sayres, L. C.; King, J. S.; Norton, M. E.; Cho, M. K. (2012). "Cell-free fetal DNA testing for fetal aneuploidy and ... Fan, H. C.; Blumenfeld, Y. J.; Chitkara, U.; Hudgins, L.; Quake, S. R. (2008). "Noninvasive diagnosis of fetal aneuploidy by ... An high degree of heterozygosity of these markers must be present for this application. Paternity testing Prenatal DNA ...
  https://en.wikipedia.org/wiki/Cell-free_fetal_DNA
*  Cell-free Fetal DNA
Thus, point mutations, copy number variations, loss of heterozygosity or aneuploidy can be detected. Digital PCR can ... Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than ... High heterozygosity of these markers must be detected for them to be applied. ... 2.5 Routine prenatal screening for aneuploidy * 2.6 Identification of Preeclampsia Preeclampsia ...
  http://theinfolist.com/php/SummaryGet.php?FindGo=Cell-free_fetal_DNA
*  Search, page 2 | eLife
... but a tumor suppressor role by preventing the aneuploidy and loss of p53 heterozygosity. ... The loss of p53 in bone cells activates critical signaling pathways; these are also essential in bone cancer. ... Activation of PTHrP-cAMP-CREB1 signaling following p53 loss is essential for osteosarcoma initiation and maintenance Mannu K ...
  https://elifesciences.org/search?for=p53&sort=relevance&order=descending&page=2
*  Merotelic kinetochore orientation versus chromosome mono-orientation in the origin of lagging chromosomes in human primary...
An increasing number of studies have shown that abnormalities such as aneuploidy and whole-chromosome loss of heterozygosity ... Loss of a single sister is more frequent than loss of both sisters. Chromosome loss events during mitosis can be visualized in ... B) Loss of a single chromatid of chromosome 7. (C) Loss of both sister chromatids of chromosome 7. When both sisters are lost ... 1A) and to discriminate single chromatid loss ( Fig. 1B) from loss of both sister chromatids ( Fig. 1C) for the two chromosomes ...
  http://jcs.biologists.org/content/115/3/507
*  Quantitative Fluorescence PCR (qf-pcr) | Thermo Fisher Scientific - AU
Figure 1: Detecting Loss of Heterozygosity (LOH) Electropherogram of a microsatellite marker in DNA from a healthy (top panel) ... Aneuploidy Detection by QF-PCR on the Applied Biosystems 3500xL Genetic Analyzer ... Applications that utilize this technique include screening for loss of heterozygosity (LOH) using microsatellites or SNPs, ... Aneuploidy Detection by QF-PCR on the Applied Biosystems 3500xL Genetic Analyzer ...
  https://www.thermofisher.com/au/en/home/life-science/sequencing/fragment-analysis/quantitative-fluorescence-pcr.html
*  Macrogen Online Sequencing Order System
Relative Fluorescent Quantization - Loss of Heterozygosity (LOH), Aneuploidy Assays, and Large Chromosomal Deletion Detection. ...
  http://dna.macrogen.com/eng/support/ces/customized_seq_intro.jsp
*  Genotype - Wikipedia
... trisomy or aneuploidy, and loss of heterozygosity (LOH). MSI and LOH in particular have been associated with cancer cell ... Serensits, Pamela; He, Haiguo, Ning, Wan; Liu, Jonathan (March 2007). "SoftGenetics Application Note - Loss of Heterozygosity ... The most common chromosomal aneuploidy is a trisomy of chromosome 21 which manifests itself as Down syndrome. Current ...
  https://en.wikipedia.org/wiki/Genotype
*  DNA- methylation changes in a human cell model of breast cancer progression
... loss of heterozygosity, chromosomal rearrangements, and overall aneuploidy [8]. Alternative mechanisms are represented by DNA ... Loss of chromosome 8p, where NRG1 is located, is one of the most frequent genomic events reported in epithelial cancers ... including the loss of methylation at the promoters of previously silenced genes [12]. Another chemical agent commonly used to ... colon and pancreatic cancer reveals a complex pattern of loss, gain and translocation. Oncogene. 2006;25:5693-5706. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2872054/?lang=en-ca
*  Plus it
... loss of heterozygosity, mutation) and ploidy abnormalities (aneuploidy, tetraploidy) within each Barrett's esophagus segment of ... Loss of Heterozygosity.. LOH analysis was performed on the flow-purified fractions, as described previously (32 , 34) yielding ... A Cox proportional hazards regression indicates that the sizes of clones with p53 loss of heterozygosity (relative risk = 1.27x ... Galipeau PC, Prevo LJ, Sanchez CA, Longton GM, Reid BJ Clonal expansion and loss of heterozygosity at chromosomes 9p and 17p in ...
  http://cancerres.aacrjournals.org/content/64/20/7629
*  Stress-Induced Loss of Heterozygosity in Candida: a Possible Missing Link in the Ability to Evolve
Dr Jeckyll and Mr Hyde: role of aneuploidy in cellular adaptation and cancer. Curr. Opin. Cell Biol. 22:809-815 [PMC free ... Stress alters rates and types of loss of heterozygosity in Candida albicans. mBio 2(4):e00129-11 [PMC free article] [PubMed] ... Stress-Induced Loss of Heterozygosity in Candida: a Possible Missing Link in the Ability to Evolve. Susan M. Rosenberg ... Stress-induced loss of heterozygosity in Candida: a possible missing link in the ability to evolve. mBio 2(5):e00200-11. doi: ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3175628/?lang=en-ca
*  Aneuploidy and malignancy: an unsolved equation | Journal of Clinical Pathology
In the aneuploid cell, there may be a loss of heterozygosity resulting in the phenotypic expression of mutated tumour ... The loss or gain of one or more chromosomes is known as aneuploidy. Normal human somatic cells have 23 pairs of chromosomes. ... TYPES OF ANEUPLOIDY. In the case of aneuploidy, the cell may gain or lose one or more chromosomes. Cells with less than a ... ANEUPLOIDY AND CARCINOGENESIS. It is still not clear whether aneuploidy is simply a non-specific state that occurs ...
  http://jcp.bmj.com/content/57/12/1245
*  Hepatocellular Carcinoma Caused by Loss of Heterozygosity in Lkb1 Gene Knockout Mice | Cancer Research
... increased aneuploidy, chromosomal breakage, and translocations (17 , 18) . However, precise molecular mechanisms for HCC ... Hepatocellular Carcinoma Caused by Loss of Heterozygosity in Lkb1 Gene Knockout Mice Masayuki Nakau, Hiroyuki Miyoshi, Michael ... Western blotting and PCR analyses showed loss of Lkb1 heterozygosity in all of the HCC tissues examined, indicating a tumor ... 3 The abbreviations used are: PJS, Peutz-Jeghers syndrome; LOH, loss of heterozygosity; RT-PCR, reverse transcription-PCR; TGF ...
  http://cancerres.aacrjournals.org/content/62/16/4549
*  Rhode Island Mesothelioma Information | Surviving Mesothelioma
A mouse centromeric DNA probe will be used to detect chromosomal aneuploidy and structural aberrations. Loss of heterozygosity ... p53- deficient mice will be used to determine whether heterozygosity at this gene locus accelerates progression from focal to ...
  https://survivingmesothelioma.com/mesothelioma-locations/mesothelioma-united-states/rhode-island/
*  p57KIP2 Expression and Loss of Heterozygosity during Immortal Conversion of Cultured Human Mammary Epithelial Cells | Cancer...
DNA aneuploidy, and poor prognosis in primary breast tumors (6, 7, 8) . The p57 gene has been found to be imprinted with ... p57KIP2 Expression and Loss of Heterozygosity during Immortal Conversion of Cultured Human Mammary Epithelial Cells Tarlochan ... p57KIP2 Expression and Loss of Heterozygosity during Immortal Conversion of Cultured Human Mammary Epithelial Cells ... 3 The abbreviations used are: CDK, cyclin-dependent kinase; CKI, CDK inhibitor; LOH, loss of heterozygosity; EL, extended life ...
  http://cancerres.aacrjournals.org/content/59/20/5112
*  Scientists Hope Cloning Will Save Endanger...
A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ... A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ...
  https://www.scoop.it/t/amazing-science/p/3281387561/2012/11/10/scientists-hope-cloning-will-save-endangered-animals
*  A Strange Computer Promises Great Speed | Amazi...
A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ... A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ...
  https://www.scoop.it/t/amazing-science/p/3998777234/2013/03/22/a-strange-computer-promises-great-speed
*  2013 Genomics: The Era Beyond the Sequencing of...
A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ... A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ...
  https://www.scoop.it/t/amazing-science/p/3997321043/2013/02/21/2013-genomics-the-era-beyond-the-sequencing-of-the-human-genome-francis-collins-craig-venter-eric-lander-et-al
*  Wobbly Alien Planet with Wild Seasons Found by ...
A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ... A common feature is the extensive loss of heterozygosity, which represents an essential source of inter-individual variation in ... Although domesticated isolates exhibit high variation in ploidy, aneuploidy and genome content, genome evolution in wild ...
  https://www.scoop.it/t/amazing-science/p/4015837108/2014/02/12/wobbly-alien-planet-with-wild-seasons-found-by-nasa-telescope
*  Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the...
Loss of heterozygosity occurs via mitotic recombination in Trp53+/- mice and associates with mammary tumor susceptibility of ... aneuploidy) in p53 null normal mammary epithelial cells. FASEB J. 2000, 14: 2221-2229. 10.1096/fj.00-0165com.View ArticlePubMed ... with mammary tumors occurring only on a BALB/c genetic background and showing a high frequency of loss of heterozygosity [61]. ... Interestingly, progesterone is necessary and favors aneuploidy in this model [62]. In the irradiation model, the increased ...
  https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1660
*  TP53mutation status and gene expression profiles are powerful prognostic markers of breast cancer | Breast Cancer Research |...
Loss of heterozygosity on the short arm of chromosome 17 is associated with high proliferative capacity and DNA aneuploidy in ... Jones MH, Nakamura Y: Detection of loss of heterozygosity at the human TP53 locus using a dinucleotide repeat polymorphism. ... Skotheim RI, Diep CB, Kraggerud SM, Jakobsen KS, Lothe RA: Evaluation of loss of heterozygosity/allelic imbalance scoring in ... ILC* is the 'typical lobular' type previously described [36]. IHC, immunohistochemistry; LOH, loss of heterozygosity. ...
  https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr1675
*  Somatic evolution in cancer - Wikipedia
... loss of heterozygosity (LOH),[35] Copy number variation (detected both by comparative genomic hybridization (CGH),[32] and ... array CGH,[38]) and karyotypic variations including chromosome structural aberrations and aneuploidy.[33][34][39][40][41] ... have used parsimony to reconstruct the relationships between biopsy samples based on loss of heterozygosity.[79] Phylogenetic ... while in most cases the loss is due to methylation of its promoter region.[50] Similarly, when loss of expression of the DNA ...
  https://en.wikipedia.org/wiki/Somatic_evolution_in_cancer