*  Spatiotemporal loss of K+ transport proteins in the developing cochlear lateral wall of guinea pigs with hereditary deafness -...
... between fibrocytes in the spiral ligament of the E50 gw/gw cochlea was observed. The barrier composed of tight junctions ... Immunohistochemical analysis of the gw/gw cochlea showed loss of the tight junction protein CLDN11 in strial basal cells from ... RNAlater facilitates microdissection of sensory cell-enriched samples from the mouse cochlea for transcriptional analyses, ... Michael C Kelly, Ping Chen, Development of form and function in the mammalian cochlea, Current Opinion in Neurobiology, 2009, ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1460-9568.2007.05994.x/abstract?globalMessage=0
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... Such analysis of two or more genes needs to be complemented with in situ hybridization of other genes, or immunohistochemistry ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ... or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary ...
  https://www.jove.com/visualize/abstract/25706271/integrated-annotation-analysis-situ-hybridization-images-using-imanno
*  KAKEN - Research Projects | Immunohistochemical study for localization of G-protein and P-glycoprotein in the inner ear ...
Immunoreactivity in the spiral ligament was light. In the cochlear nerve and spiral ganglionic fibers, immunoreactivity against ... By Western immunoblot analysis, P-gp of 170 kDa was detected at a high level in isolated brain capillaries and at low levels in ... P-gp was found only in the capillary endothelial cells of the cochlea and vestibule. The pattern of P-gp immunostaining in the ... Western blot analysis in cochlear tissue with the same antibodies used in the immunohistochemistry demonstrated a predominant ...
  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671847/
*  Distinct Expression Pattern of a Deafness Gene, KIAA1199, in a Primate Cochlea
Expression of KIAA1199 has been reported in the fibrocytes of the spiral ligament and the spiral limbus with a transient ... Expression analysis of KIAA1199 protein in the cochlea has been performed in mice and rats [1, 11], where different ... a and b) KIAA1199 expression is observed in the lateral wall of the cochlea, sensory epithelium, spiral limbus, and spiral ... LW: lateral wall of cochlea, OC: organ of Corti, SL: spiral limbus, SGN: spiral ganglion neuron, and RM: Reissner's membrane. ...
  https://www.hindawi.com/journals/bmri/2016/1781894/
*  Acceleration of Age-Related Hearing Loss by Early Noise Exposure: Evidence of a Misspent Youth | Journal of Neuroscience
... spiral ligament, and spiral limbus. The examiner was blind to age-exposure status. ... Analysis included estimates of inner and outer hair cell loss, spiral ganglion cell loss, and loss of type IV fibrocytes. Each ... Keithley EM, Feldman ML (1979) Spiral ganglion cell counts in an age-graded series of rat cochleas. J Comp Neurol 188:429-442. ... For example, the loss of spiral ligament fibrocytes seen by 2 weeks after exposure in our animals may not occur after a 5 min ...
  http://www.jneurosci.org/content/26/7/2115.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... Since OHC motile responses are an essential component of the cochlear amplifier, the qualitative and quantitative analysis of ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ... or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary ...
  https://www.jove.com/visualize/abstract/23284995/neurotrophin-gene-therapy-for-sustained-neural-preservation-after
*  Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV | (2011) | Publications | Spie
... the spiral ligament, the spiral limbus, and the modiolus could be clearly identified. When we imaged intact adult cochleae, we ... Dynamic analysis of a small artery of a human finger by optical coherence tomography Author(s): Mitsuo Kuwabara; Natsuki ... to image the vibration of various micro-structures of the cochlea at the same time. An excised cochlea of a guinea pig was ... We present sensitivity analysis of these setups and images of in vivo skin. Advantages of both common path arrangements include ...
  http://spie.org/Publications/Proceedings/Volume/7889
*  Otosclerosis - Wikipedia
It may result from direct injury to the cochlea and spiral ligament from the lytic process or from release of proteolytic ... One genome-wide analysis associates otosclerosis with variation in RELN gene. Loci include: The pathophysiology of otosclerosis ... well documented instances of sclerotic lesions directly obliterating sensory structures within the cochlea and spiral ligament ... The best understood mechanism is fixation of the stapes footplate to the oval window of the cochlea. This greatly impairs ...
  https://en.wikipedia.org/wiki/Otosclerosis
*  Frontiers | Regulation of Fn14 Receptor and NF-κB Underlies Inflammation in Meniere's Disease | Immunology
Signaling analysis predicted several TNF-related pathways, being the TWEAK/Fn14 pathway the top candidate (p= 2.42 x 10-11). ... Signaling analysis predicted several TNF-related pathways, being the TWEAK/Fn14 pathway the top candidate (p= 2.42 x 10-11). ... We have identified the first locus, at 6p21.33, suggesting an association with bilateral MD (meta-analysis leading signal ... We have identified the first locus, at 6p21.33, suggesting an association with bilateral MD (meta-analysis leading signal ...
  https://www.frontiersin.org/articles/10.3389/fimmu.2017.01739/full
*  A 3-D model of membrane specializations between human auditory spiral ganglion cells
... in connective tissue cells of the spiral ligament and spiral limbus, as well as in the basal and intermediate cell layers of ... In the immunohistochemical studies on the human cochlea, ZO-1 was expressed in tissues lining scala media, thus contributing to ... This thesis presents results of analyses of human cochlear tissue and specimens obtained during neurosurgical transpetrosal ... 1. The Human Spiral Ganglion. Open this publication in new window or tab ,,The Human Spiral Ganglion. Tylstedt, Sven. Umeå ...
  http://umu.diva-portal.org/smash/record.jsf?pid=diva2:144470
*  Dopaminergic Signaling in the Cochlea: Receptor Expression Patterns and Deletion Phenotypes | Journal of Neuroscience
RT-PCR analysis on agarose gels (Fig. 2A) revealed bands for D1 and D5 as well as D2 receptors in spiral ganglion cells and ... 2001) Spiral ligament pathology: a major aspect of age-related cochlear degeneration in C57BL/6J mice. J Assoc Res Otolaryngol ... 1997) Quantitative analysis of dopamine receptor messages in the mouse cochlea. Mol Brain Res 44:151-156. ... Special attention was paid to the basal-turn hair cells and to the type-IV fibrocytes in the spiral ligament, which are the ...
  http://www.jneurosci.org/content/32/1/344.long
*  Presbycusis - Wikipedia
GPx1, an isoform of GPx, is predominantly expressed in stria vascularis, cochlea, spiral ligament, organ of Corti, and spiral ... a comparative analysis of published threshold data." Audiology 1979; 18(4): YOLO 320-334 [1] E. Van Eyken, G. Van Camp, L. Van ... The cochlea is tonotopically mapped in a spiral fashion, with lower frequencies localizing at the apex of the cochlea, and high ... Heredity: factors like early aging of the cochlea and susceptibility of the cochlea for drug insults are genetically determined ...
  https://en.wikipedia.org/wiki/Presbycusis
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... DNA assessment or chromosomal analysis 2,3. An especially challenging application of LMD is transcriptome analysis, which, due ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ... or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary ...
  https://www.jove.com/visualize/abstract/20174573/laser-microdissection-sensory-organ-precursor-cells-drosophila
*  The Role of the Transcription Factor Foxo3 in Hearing Maintenance: Informed Speculation on a New Player in the Cochlea
These age-related mitochondrial mutations are found in spiral ganglion neurons, organ of Corti cells, and the spiral ligament ... Qualitative analysis of human postmortem samples reveals cellular pathology similar to animal models. Loss of outer hair cells ... in combination with supporting cells and the cells of the spiral ligament. No regeneration has been reported for lost cells in ... fewer spiral ganglion neurons, with losses more evident in the basal region [23, 24]. Total spiral ganglion losses increase to ...
  https://www.hindawi.com/journals/bmri/2016/1870675/
*  Raúl Hinojosa - Association for Research in Otolaryngology
Unexpectedly, spiral ganglion cells may be reduced in parts of the cochlea with normal-appearing hair cells, implying that ... Hinojosa R. (1972) Electron microscope studies of the stria vascularis and spiral ligament after ferritin injection. Acta ... Proteomic analysis of formalin-fixed celloidin-embedded whole cochlear and laser microdissected spiral ganglion tissues. Acta ... John Lindsay. Analysis of these temporal bones has yielded several key insights. For example, in a patient with connexin 26- ...
  http://www.aro.org/page/Hinojosa
*  Spiral ligament fibrocyte-derived MCP-1/CCL2 contributes to inner ear inflammation secondary to nontypeable H. influenzae...
Statistical analysis was performed using Student's t test and Fisher's exact test with significance considered to be a p value ... After entering the cochlea from the middle ear through the round window membrane, bacterial molecules in the perilymph of the ... Cochlear resident macrophages are found to be localized in the spiral ligament and the spiral ganglion area [44], but further ... Immunolabeling shows MCP-1/CCL2 is highly expressed in the spiral ligaments (white arrows) and spiral limbus as well as the ...
  https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-10-314
*  Age-Related Cochlear Synaptopathy: An Early-Onset Contributor to Auditory Functional Decline | Journal of Neuroscience
1979) Spiral ganglion cell counts in an age-graded series of rat cochleas. J Comp Neurol 188:429-442, doi:10.1002/cne.901880306 ... 2001) Spiral ligament pathology: a major aspect of age-related cochlear degeneration in C57BL/6 mice. J Assoc Res Otolaryngol 2 ... 1990) Afferent and efferent innervation of the cat cochlea: quantitative analysis with light and electron microscopy. J Comp ... For quantification of spiral ganglion cells, cochleae were fixed as described above, postfixed in 2% formaldehyde and 2% ...
  http://www.jneurosci.org/content/33/34/13686.long
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... The data-analysis code harvests the data several times a day and subjects it to statistical and graphical analyses, which are ... The data analysis and graphing routines are written in a MATLAB-based language created to simplify greatly the analysis of ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ...
  https://www.jove.com/visualize/abstract/24967577/autapse-induced-spiral-wave-in-network-of-neurons-under-noise
*  Three-dimensional current flow in a large-scale model of the cochlea and the mechanism of amplification of sound | Journal of...
... cotransporter back into the type II fibrocytes of the spiral ligament and from there back via the stria vascularis into the ... I. Analysis without reactive elements. Hear Res. 12, 89-119. doi:10.1016/0378-5955(83)90120-X. ... The K+ circulation in the cochlea is thought to be tightly regulated in the inner compartment of the cochlea by a transport ... end of the cochlea (Mammano & Ashmore 1996; Preyer et al. 1996). In both mammalian and turtle cochleae, it is highly probable ...
  http://rsif.royalsocietypublishing.org/content/6/32/279
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... Analysis of Nephron Composition and Function in the Adult Zebrafish Kidney. Authors: Kristen K. McCampbell, Kristin N. Springer ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ... or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary ...
  https://www.jove.com/visualize/abstract/23240046/depletion-resident-macrophages-does-not-alter-sensory-regeneration
*  Plus it
Artifacts of the electrode in cochlea implantation and limits in analysis of deep insertion in cone beam tomography (CBT). Eur ... tearing of the basilar membrane or spiral ligament, and deviation of the electrode array into the scala media or scala ... Statistical Analysis. In the group of patients with regular electrode insertions, statistics were calculated and used for ... According to the histologic study of Marx et al,11 the electrode position within the cochlea can be reliably identified. When ...
  http://www.ajnr.org/content/36/2/372
*  Morphological results. The figure shows the results obt | Open-i
... close to the stria vascularis and spiral ligament (see high magnification). Mentions: Morphological analysis of cochlear ... Grafting and early expression of growth factors from adipose-derived stem cells transplanted into the cochlea, in a Guinea pig ... close to the stria vascularis and spiral ligament (see high magnification). ... Mentions: Morphological analysis of cochlear sections revealed no signs of relevant traumatic damage to the basilar membrane, ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4202717_fncel-08-00334-g004&req=4
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... The protocol is most advantageous for parallel analysis of multiple samples (e.g. single cells, dynamic analysis following ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ... or as a further dissected micro-isolate that lacks the spiral limbus and spiral ganglion neurons. Using this method, primary ...
  https://www.jove.com/visualize/abstract/24804771/a-lack-immune-system-genes-causes-loss-high-frequency-hearing-but
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... and subsequent isolation of the cochlea, spiral ligament, and organ of Corti. Once isolated, the sensory epithelium can be ... cytometric bead array and Western blot analysis), gene expression analysis (real-time PCR), toxicological/drug analysis and ... These analyses include, but are not limited to, structural/morphological analysis (confocal, scanning and transmission electron ... the sensory epithelium for audition is located along the spiraling organ of Corti that resides within the conch shaped cochlea ...
  https://www.jove.com/visualize/abstract/21479267/functional-changes-snail-statocyst-system-elicited
*  The protective effects of whortleberry extract against cisplatin-induced ototoxicity in rats | Brazilian Journal of...
... spiral ligament; Sg, spiral ganglion; black arrow, spiral ganglion cells (hematoxylin eosin stain, ×40). ... The lateral wall of the cochlea was removed, and the cochlea was fixed with a 2.5% glutaraldehyde solution after a slow ... Statistical analysis. The data was analyzed with SPSS version 15.0 for Windows (SPSS Inc., Chicago, IL, USA). The results were ... spiral ligament; Sg, spiral ganglion; black arrow, spiral ganglion cells; (hematoxylin eosin stain, ×40). ...
  http://rborl.org.br/en-the-protective-effects-whortleberry-extract-avance-resumen-S1808869417301908