*  Small RNA Detection by in Situ Hybridization Methods - pdf descargar
Small RNA Detection by in Situ Hybridization Methods. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
*  Comparison of Fluorescence In Situ Hybridization and Chromogenic In Situ Hybridization for Low and High Throughput HER2 Genetic...
The purpose was to evaluate and compare 5 different HER2 genetic assays with different characteristics that could affect the performance to analyze the human epidermal growth factor 2 (HER2) gene copy number under low and high throughput conditions. The study included 108 tissue samples from breast cancer patients with HER2 immunohistochemistry (IHC) results scored as 0/1+, 2+, and 3+. HER2 genetic status was analysed using chromogenic in situ hybridization (CISH) and fluorescence in situ hybridization (FISH). Scoring results were documented through digital image analysis. The cancer region of interest was identified from a serial H&E stained slide following tissue cores were transferred to a tissue microarrays (TMA). When using TMA in a routine flow, all patients will be tested for HER2 status with IHC followed by CISH or FISH, thereby providing individual HER2 results. In conclusion, our results show that the differences between the HER2 ...
*  In Situ Hybridization - Application Of The Probe For Dna Or Rna To Tissues Or Cells - Chromosome, Location, and Detect -...
In situ hybridization allows us to learn more about the geographical location of, for example, the messenger RNA (mRNA) in a cell or tissue. It can also tell us where a gene is located on a chromosome. Obviously, a detection system must be built into the technique to allow the cytochemist to visualize and map the geography of these molecules in the cells in question.. When in situ hybridization was first introduced, it was applied to isolated cell nuclei to detect specific DNA sequences. Early users applied the techniques to isolated chromosomal preparations in order to map the location of genes in those chromosomes. The technique has also been used to detect viral DNA in an infected cell. In situ hybridization of RNA has also been used to show that RNA synthesis (transcription) occurs in the nucleus, while protein synthesis (translation) occurs in the cytoplasm.. ...
*  Variant three-way translocation of inversion 16 in AML-M4Eo confirmed by fluorescence in situ hybridization analysis
All information about the latest scientific publications of the Clínica Universidad de Navarra. Variant three-way translocation of inversion 16 in AML-M4Eo confirmed by fluorescence in situ hybridization analysis
*  Seminar University Hospital Bonn, Germany | In Situ Hybridization, RNA-ISH | ACDBio
Visualize Gene Expression & Genetic Variations in Tissues:. Applications of RNAscope® and BaseScopeTM ISH technology. The nervous system consists of numerous specialized cell types that remain to fully cataloged and characterized at the molecular level. Due to the high degree of structural and functional heterogeneity and the intricate spatial organization of these cells, it is of special importance to analyze gene expression in the presence of full morphological and spatial contexts. Due to the lack of specific antibody reagents, especially for lncRNAs, G-protein coupled receptors (GPCRs), and ion channels, mapping of specific transcripts by in situ hybridization offers an excellent alternative approach. The RNAscope® assay provides a powerful method to detect gene expression within the spatial and morphological tissue context. BaseScopeTM is a novel in situ hybridization technology that allows visualization of splice junctions between ...
*  in situ hybridization
Hi hybridizers, i am trying to find the best method of fixation for plant cell culture material in in situ hybridization experiments with Dig-labeled riboprobes. A fixation in 3-4% FA pH9 leads to nice signal after detection with Mouse-Anti-Dig and Anti-Mouse-Fitc but to poor preservation of cell structure. After Fixation in 3-4% FA and 0.1% GA the preservation is nice, but due to the autofluorescence of GA-fixed cells i have to treat the cells with borohydride (5mg/ml) for quenching. This treatment seems to eat away not only the background but also the signal. Is this due to the reaction of borohydride with all double bonds (nothing left to hybridize against) ? Who has a nicer fixation protokoll, so that the preservation survives all the high temperature inkubations of in situ experiments? Any suggestions welcome! Thank you in advance. Uwe ...
*  oligo in situ hybridizations
I am trying to set up doing in situ hybridization on paraformaldehyde fixed sections from mouse embryos using a 24mer oligonucleotide. I would appreciate tips on the best way to do this: end labelling or A-tailing. 3H or 35S as label. Also the best way to work out hybridization and washing conditions. All recipes gratefully received. Kathy Cheah HRMBDKC at HKUCC.BITNET ...
*  Antisense probe as negative control for in situ hybridization - Molecular Biology
Hi guys! So I'm doing some in situ hybridization experiments, and I want to design a better negative control, a labelled probe that shouldn't bind to the mRNA transcribed from my gene of interest.. I'm not trained as a molecular biologist, but I am keen to learn these things! So to hybridize to the mRNA of my gene, I design primers that are going to amplify a bit of DNA that is complementary to it, and then in vitro transcribe RNA that is complementary to that, and so it binds to the mRNAs of my gene, yeah?. How would I go about designing an anti-sense probe? What would this bind to? What should I amplify off of (i.e. what should the primers anneal to?)?. Any help or clues would be much appreciated. Gracias!. ...
*  Re: Fixative Buffering
Jacques Paysan wrote: , Dear Histonetters , , In theory, formaldehyde should mainly react with unprotonated amino- and , guanidyl-groups of tissue proteins. Since the degree of protonation of , these groups for a particular protein depends on the pH during fixation the , fixative buffering should be an interesting parameter to control the , fixation process, particularly if for example an epitope is fixation , sensitive. Recently, I found a publication in Nucl. Acid Res. by Basyuk et , al (28: e46, 2000) where an alkaline fixation procedure was described, , improving in situ hybridization signals dramatically when fixing at pH 9.5. , We tested this and got similar results. However, it appeared to us that , fixing in PBS at pH 9.5 sounds somewhat unreasonable because PBS should have , almost no buffering capacity at this range. We used carbonate buffer , insted, which worked well, but we would like to extent this study to other , pH-values. Since I'm not much into buffer ...
*  InSitu Hybridization For MRNA Localization - WriteWork
In Situ Hybridization for mRNA Localization Purpose: In Situ hybridization for mRNA localization is used to identify the position of target mRNA in the cell or tissues being examined(Smith, 2001).Methods: In order for in situ hybridization for localiza...
*  調査レポート | In situハイブリダイゼーション(ISH)の世界市場予測(~2021):技術別(FISH、CISH)、用途別、需要先別 | MarketsandMarkets
The global in situ hybridization market is segmented on the basis of technique, application, and end user. By technique, the market is categorized into fluorescent in situ hybridization (FISH) and chromogenic in situ hybridization (CISH). The FISH segment is expected to command the largest share of the global market, by technique in 2016. This segment is also projected to grow at the highest CAGR during the forecast period (2016-2021). This can be attributed to factors such as like high resolution of this technique, rising adoption in research activities & laboratories to diagnose cancer, chromosomal abnormalities, and infectious diseases, among others.. By application, the in situ hybridization market is categorized into cancer diagnosis, cytology, neuroscience, immunology, and infectious diseases. The cancer diagnosis segment is expected to command the largest share of the ...
*  Fluorescence In Situ Hybridization (FISH) | Thermo Fisher Scientific
Multiplex fluorescence in situ hybridization (FISH) enables you to assay multiple targets and visualize colocalized signals in a single specimen. Using spectrally distinct fluorophore labels for each hybridization probe, this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. Life Technologies offers FISH Tag™ detection kits for routine analysis, and TSA kits for very rare or low-abundance targets.
*  Expression of Raldh1. In situ hybridisation was perform | Open-i
Expression of Raldh1. In situ hybridisation was performed on wholemount embryos with DIG- or fluorescein-labelled riboprobes. Unless otherwise stated, rostral i
*  Localization during development of alternatively spliced forms of cytotactin mRNA by in situ hybridization. | JCB
Cytotactin, an extracellular glycoprotein found in neural and nonneural tissues, influences a variety of cellular phenomena, particularly cell adhesion and cell migration. Northern and Western blot analysis and in situ hybridization were used to determine localization of alternatively spliced forms of cytotactin in neural and nonneural tissues using a probe (CT) that detected all forms of cytotactin mRNA, and one (VbVc) that detected two of the differentially spliced repeats homologous to the type III repeats of fibronectin. In the brain, the levels of mRNA and protein increased from E8 through E15 and then gradually decreased until they were barely detectable by P3. Among the three cytotactin mRNAs (7.2, 6.6, and 6.4 kb) detected in the brain, the VbVc probe hybridized only to the 7.2-kb message. In isolated cerebella, the 220-kD polypeptide and 7.2-kb mRNA were the only cytotactin species present at hatching, indicating that the 220-kD polypeptide is encoded by the 7.2-kb ...
*  Histonet] In Situ Hybridization Blue Background problem
I have an In Situ Hybridization question for you guys and gals. I'm currently performing In Situ HPV and EBV tests using an automated machine called the XT by Ventana. The tissue is parrifin embedded, cut at 4 microns and place on charged slides. NBT/ BCIP is used as our chromogen. I am beginning to notice some background staining that varies in intensity. When it occurs on our QC slide, it can be strong enough to fail a run. The background resembles a 'blue haze' that can cover the tissue making interpretation difficult. At other times the blue haze surrounds the tissue rather than covering it which makes me believe that there could be some sort of hydrophobic or other chemical rxn taking place to shield the tissue. I'm not certain if these two problems are related, but both occur completely random and always vary in intensity. If I can find the culprit of this problem it would lead to cleaner slides and more conclusive resulting. If anybody can help it ...
*  Introduction to RNAscope® Probes: Identifying the right probe for your research | In Situ Hybridization, RNA-ISH | ACDBio
RNAscope® assay is an innovative and proprietary RNA in situ hybridization (ISH) assay based on ACD's patented technology with signal amplification and simultaneous background noise suppression.
*  Contact | In Situ Hybridization, RNA-ISH | ACDBio
Advanced Cell Diagnostics is a leader in the emerging field of molecular pathology, developing cell-based and tissue-based diagnostic tests for personalized medicine, focusing on RNA ISH (in situ Hybridization).
*  In situ hybridization of eye from bioassay. Example of | Open-i
In situ hybridization of eye from bioassay. Example of confocal photomictographs of the fasciculated zone of the eye of a challenged shrimp specimen from test 2
*  Fluorescence in situ Hybridization(荧光原位杂交)
简介荧光原位杂交(fluorescence in situ hybridization,FISH)是在20世纪80年代末在放射性原位杂交技术的基础上发展起来的一种非放射性分子细胞遗传技术,以荧光标记取代同位素标记而形成的一种新的原位杂交方法。探针首先与某种介导分子(reporter molecule)结合,杂交后再通过免疫细胞化学过程连接上荧光染料。FISH的基本原理是将DNA(或RNA)探针用特
*  Practical in situ hybridization | Oxfam GB | Oxfam's Online Shop
Buy Practical in situ hybridization, Oxfam, Trude schwarzacher and Pat Heslop-Harrison, 9781859961384, 9781859961384, Books, Science and Nature
*  Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system | BMC Neuroscience | Full Text
In this study we used quantitative RT-PCR (qPCR) in a large panel of adult rat brain and peripheral tissues (Figure 1), to further refine the expression profile of the Slc6a17 gene. The highest expression levels of Slc6a17 mRNA were found in hindbrain, various brain cross sections, cerebellum, spinal cord, brain stem and hypothalamus, while very low or no expression was seen in the peripheral tissues with the exception of epididymis. Consequently, the Slc6a17 transporter is highly and selectively expressed in the CNS of adult rat.. Abundant mRNA expression of Slc6a17 in adult and embryonic rat CNS has previously been shown using in situ hybridization. Consistent results indicated restricted expression exclusively in neurons, both glutamatergic and subsets of GABAergic [4, 9, 15-17]. Our results from in situ hybridization (Figure 3) shows that the mouse Slc6a17 gene has similar expression pattern as previously seen in rat, with high ...
*  Whole-mount in situ hybridization (Conlon lab) - Xenbase
AP Buffer 1 M Tris pH 9.5 100 mM 5 ml 1 M MgCl2 50 mM 2.5 ml 0.5 M NaCl 100 mM 10 ml Tween 20 0.1% 50 μl Levamisol 5 mM 60 mg ddH2O to 50 ml - 5 min @ RT in AP Buffer Staining: - Add 1-2 ml BM Purple AP Substrate (Roche 1442074) - KEEP IN THE DARK UNTIL BLEACHING STEP BELOW TO AVOID BACKGROUND STAINING!! - Let stain anywhere from 1 to 8 hours until stain is at desired level - Rinse with PBS - 5 min @ RT in PBS - 5 min @ RT in PBS - 5 min @ RT in PBS - 1hr @ RT in MEMFA - 1 hr in MeOH (can be stored @ 4°C here) ...
*  In Situ Hybridization - Kits : Heat Pretreatment Solution Citric
Distributor of Immunohistochemistry IHC antibodies, Flow Cytometry, Molecular Biology, Staining Kits, Reagents, Detection Systems Ancillary reagents
*  Gene Expression Literature Detail
J:92177 Powles N, Babbs C, Ficker M, Schimmang T, Maconochie M, Identification and analysis of genes from the mouse otic vesicle and their association with developmental subprocesses through in situ hybridization. Dev Biol. 2004 Apr 1;268(1):24-38 ...
*  Additional developmental defects resulting from amphime | Open-i
Additional developmental defects resulting from amphimedine treatment. In situ hybridization was performed on control (A, C, E, G, I, K, M, O, Q) and treated (B