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*  Promising HPV Test: Fast, Precise and Low-Cost
A reading device measures the amount of hybridized viral DNA on the beads. By their characteristic color, the beads tell us ... small DNA fragments each of which is typical for a specific virus type. If the DNA sequences of the viral DNA under study and ... They first isolate the viral genetic material from a tissue sample, amplify and label it. The enriched DNA material is ... The enriched DNA material from 500 tissue samples can be tested for up to 100 HPV types - all in just one day. Since the test ...
  http://www.innovations-report.com/html/reports/medicine-health/report-55001.html
*  Material Search Results
Viral load quantification is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate... see ... Evaluation of Biofield Modality on... Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses ... In vitro Evaluation of Biofield... In vitro Evaluation of Biofield Treatment on Viral Load Against Human Immunodeficiency-1 and ... In vitro Evaluation of Biofield Treatment on Viral Load Against Human Immunodeficiency-1 and Cytomegalo Viruses ...
  https://www.merlot.org/merlot/materials.htm?nosearchlanguage=&pageSize=&page=8&category=2608&sort.property=dateCreated
*  Our Inner Viruses: Forty Million Years In the Making - Phenomena
This process has generated a huge amount of viral DNA in the human genome. We carry about 100,000 pieces of DNA that came from ... Viral DNA or RNA that benignly settles itself into a vector or inside human DNA itself turns into a sleeping gene, enjoying the ... But what I want to know is if 5% of human DNA is viral and "unconnected" to effective human DNA, what is it doing there? Is it ... The scientists can trace our viral DNA to 30 to 35 separate invasions. Once each virus established itself in our ancestors' DNA ...
  http://phenomena.nationalgeographic.com/2015/02/01/our-inner-viruses-forty-million-years-in-the-making/?replytocom=1956877
*  What are exogeneous and endogeneous controls? - PCR, RT-PCR and Real-Time PCR - BioForum
This is typically used when you need to quantify a given amount of template; for example to quantify the amount of viral DNA in ... You basically use the endogenous control to normalize the amount of DNA template in all your samples. Either one can be very ... An exogenous control is a control DNA spiked into your DNA samples. For example adding 100 ng of a 200 bp template to your cDNA ... 1) heterologous controls where you end up with two primer pairs in the tube + a spiked DNA from outside (can also be in a ...
  http://www.protocol-online.org/forums/topic/20615-what-are-exogeneous-and-endogeneous-controls/
*  Treatment of Hepatitis B Prevents Infection by Hepatitis D | HubPages
DNA test can detect the presence of viral DNA and measure the amount of DNA ( viral load ) in the patient's blood." DNA test is ... DNA alone or RNA alone cannot produce a cell or a tissue or an organ. The DNA virus or RNA virus is only a message. It is not ... It is more sensitive; it can also quantify viral DNA load in the range of 5X102 to 1X108 copies per milliliter without ... DNA is a heredity material, part of gene that is, in turn, part of chromosome. DNA and RNA always work in tandem to make an ...
  https://hubpages.com/health/Treament-for-Hepatitis-B-Prevent-Hepatitis-D
*  Plus it
Leaves 16 and 20 had trace amounts of CaLCuV DNA (Fig. 1E, lanes 10 and 11). Similar distributions of viral DNA were seen in ... Viral DNA accumulation was monitored independently in three plants. Tissue printing of viral DNA was performed by rubbing ... At this time, leaves 7, 8, and 9 contained the highest amounts of viral DNA and were larger than 1 cm in length (Fig. 1E). ... 5E). In contrast, only trace amounts of viral DNA were observed in leaves from CYCD3;1-overexpressing plants. Quantitative RT- ...
  http://www.plantphysiol.org/content/148/1/436
*  Options for HBV Vaccine Non-Responders - Hepatitis B Foundation
It also depends on your viral load - the amount of HBV DNA in your bloodstream. The higher your viral load, the more infectious ... Hello: Your immune system may be able to keep the infection mostly in-check and you may have a low viral load (HBV DNA), which ... Your doctor may also order a viral load (HBV DNA) test, which measures how much hepatitis B virus you have in your body.. Is ... Getting tested again for the surface antigen and antibody, core antibody and also for viral load (HBV DNA) should clarify your ...
  http://www.hepb.org/blog/hbv-vaccine-non-responder-alternatives/
*  Plus it
The amount of viral DNA in each sample relative to albumin was calculated using the comparative threshold cycle (CT) method, ... Viral DNA was quantified by qRT-PCR and results expressed as the percentage of control (vehicle-treated cultures considered as ... 11A). Viral replication was also diminished in the presence of the drug, with a reduction in viral titer by ∼100-fold (4.92 × ... VCD decreased the amount of virus detected in both the cerebrum and cerebellum by a very substantial amount, with an ∼100- to ...
  http://www.jneurosci.org/content/37/29/6877
*  HIV-1 capsid is involved in post-nuclear entry steps | Retrovirology | Full Text
Sub-cellular fractionation showed that small amounts of capsid accumulated in the nuclei of infected cells and C-A1 reduced the ... suggesting that Nup153 may help maintain some integrity of the viral core in the nucleus. Furthermore C-A1 increased binding of ... HIV-1 capsid influences viral uncoating and nuclear import. Some capsid is detected in the nucleus but it is unclear if it has ... the amount of viral DNA and 2LTR circular DNA. The amount of integrated viral DNA was determined by Alu-LTR TaqMan qPCR 10 days ...
  https://retrovirology.biomedcentral.com/articles/10.1186/s12977-016-0262-0
*  Inhibition of human cytomegalovirus immediate-early gene expression and replication by the ethyl acetate (EtOAc) fraction of...
... indicate that the EtOAc fraction of ESE contains active constituents that inhibit HCMV MIE enhancer/promoter activity and viral ... Among the solvent fractions tested, the EtOAc fraction of ESE markedly reduced HCMV lytic gene expression and viral replication ... At 3 days after infection, total DNA was harvested and the relative amounts of viral DNA were measured via qPCR using primers ... is the largest human herpesvirus with a linear double-stranded DNA genome of about 230 kb encoding 167 viral proteins [1, 2]. ...
  https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1941-7
*  Raltegravir inhibits murine leukemia virus: implications for chronic fatigue syndrome?
The authors speculate that by inhibiting viral DNA integration, raltegravir increases the amount of unintegrated viral DNA, ... The double-stranded viral DNA is then integrated into the chromosomal DNA of the host cell, a process accomplished by an viral ... The drug blocks the integration of viral DNA into the host genome and therefore inhibits viral replication. ... The mechanism by which the virus induces this disease is not known, but stimulation of innate immune responses by viral DNA ...
  http://www.virology.ws/2009/11/20/raltegravir-inhibits-murine-leukemia-virus-implications-for-chronic-fatigue-syndrome/comment-page-1/
*  Progress toward an HIV cure in annual special issue of AIDS Research & Human Retroviruses | EurekAlert! Science News
... capable of identifying very small amounts of viral DNA in tissue samples from HIV-infected individuals. ... Viral Immunology, and Journal of Interferon and Cytokine Research. Its biotechnology trade magazine, GEN (Genetic Engineering ...
  https://www.eurekalert.org/pub_releases/2018-01/mali-pta012518.php
*  Virology] RE: Virology Digest, Vol 29, Issue 1
... and then extract viral DNA and estimate the number of viral particles based on the amount of viral DNA present, knowing that ... parvoviruses have a single-stranded DNA genome about 5.2 kbp in size? Just a thought... Magda -----Original Message----- From: ...
  http://www.bio.net/bionet/mm/virology/2008-May/007748.html
*  The Institute for Creation Research
... great ongoing myths of evolution is that the genomes of animals and humans are littered with vast amounts of genomic viral DNA ... These alleged ancient viral sequences are thought to have entered the genome via viral infection, initially served no purpose ... over the past 100 million years of mammalian evolution-with their viral-like DNA proliferating across creatures' genomes.1 ... According to evolutionary theory, viruses have repeatedly integrated themselves into the DNA of germline cells (those that ...
  http://www.icr.org/article/8661
*  In vitro biology of fibropapilloma-associated turtle herpesvirus and host cells in Hawaiian green turtles (Chelonia mydas)
CFPHV pol DNA was preferentially concentrated in dermal fibroblasts of skin tumours and the amount of viral DNA per cell was ... if DNA sequesters by tumour size and/or cell type, (ii) whether subculturing of cells is a viable strategy for isolating CFPHV ... Our aims were to employ quantitative PCR targeted to pol DNA of CFPHV to determine (i) ... CFPHV pol DNA was preferentially concentrated in dermal fibroblasts of skin tumours and the amount of viral DNA per cell was ...
  https://pubs.er.usgs.gov/publication/70037369
*  Displaying items by tag: cell culture
The HPV early proteins are responsible for maintaining a proper amount of viral DNA inside the host cell nucleus. However, they ... may interact not with viral DNA but rather with its histones. To date, however, much of the process through which HPV DNA is ... Late proteins of HPV form the viral capsid and mediate packaging of DNA into the virion. The pentamer-forming L1 protein is the ... Proteins E1 and E2 form a complex with viral DNA, which recruits the cell replication systems. Proteins E6 and E7 are ...
  http://gentaur-worldwide.com/index.php?option=com_k2&view=itemlist&task=tag&tag=cell%20culture&Itemid=563
*  tenofovir
High Viral Loads in Men Increase the Amount of HBV DNA in Their Sperm ... We may notice a gradual rise in our viral load (HBV DNA) and/or our liver enzymes (ALT/SGPT), which indicate liver damage. ... With the help of antivirals, many patients today have undetectable viral load (HBV DNA), a relatively healthy liver and cleared ... HBV DNA) and they carry the lowest risk of drug resistance. Doctors usually prescribe antivirals when our viral load is ...
  http://www.hepb.org/blog/tag/tenofovir/
*  The potential role of microfilaments in host cells for infection with infectious spleen and kidney necrosis virus infection |...
The relative amount of viral DNA in each treatment indicated the number of virus particles that had entered the cells. Data ... Viral DNA of the supernatants was extracted to analyze the inhibition of release of virus by the compounds using Purelink Viral ... The number of viral DNA molecules was then calculated by using the equation of the straight line. p,0.05 was considered ... Total DNA of cell fractions was extracted for qPCR analysis to analyze the inhibition of viral replication by the compounds ...
  https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-10-77
*  Biofield Impact on Antibiogram of Shigella Boydii | Pearltrees
Potential Impact of Biofield on HIV Viral Load Test Abstract Viral load quantification is the amount of particular viral DNA or ... High viral load indicates that the immune system is failed to fight against viruses. The aim of this study was to evaluate the ... The viral load assay was performed on stored stock cultures of HIV infected human plasma samples before and after 7 days of ... impact of biofield treatment on HIV-1 and HCMV in terms of viral loads as surrogate marker. ...
  http://www.pearltrees.com/u/123141425-antibiogram-infections
*  Genomic DNA double-strand breaks are targets for hepadnaviral DNA integration | PNAS
... implying that the amount of integrated viral DNA in the liver may reflect the degree of overall genetic damage sustained by the ... Linear DNA was highly preferred over relaxed circular DNA as the ligation substrate. The viral-cell junctions produced at the ... Integration depended on the production of viral double-stranded linear DNA and the expression of I-SceI, and integrated DNA was ... viral DNA was maintained in the cell population through multiple transfers even though unintegrated replicating viral DNA was ...
  http://www.pnas.org/content/101/30/11135
*  HIV-1 Vaccine Test in Uninfected Adult Volunteers - Full Text View - ClinicalTrials.gov
... the viral DNA instructs the body to make small amounts of some HIV proteins. This study will see if the body then creates an ... Educational materials on DNA vaccines will be reviewed with and provided to participants before enrollment into the study. ... Tighe H, Corr M, Roman M, Raz E. Gene vaccination: plasmid DNA is more than just a blueprint. Immunol Today. 1998 Feb;19(2):89- ... The vaccine in this study is made from DNA (genetic material) of two HIV proteins called 'gag' and 'pol.' Injected into a human ...
  https://clinicaltrials.gov/ct2/show/study/NCT00009685?view=results
*  Phage 29 phi protein p1 promotes replication by associating with the FtsZ ring of the divisome in Bacillus subtilis. - PubMed ...
Data (μg/mL of viral DNA) were normalized according to the same amount of genomic DNA of the infected strains. Statistical ... The cellular length of A and B was measured with Fiji software (ImageJ) (). (C) The amount of intracellular accumulated ϕ29 DNA ... Synthesis of protein p1 increases B. subtilis cellular length and the bacterial enlargement promotes viral DNA accumulation. (A ... viral DNA, bacterial nucleoid, and membrane are not drawn to scale. ...
  https://www.ncbi.nlm.nih.gov/pubmed/23836667
*  Agricultural biotechnolgies a boon for family farmers, say experts
The use of polymerase chain reaction (PCR)-a tool to make more DNA until there is a sufficient amount of the viral DNA for ... Detection of viral diseases in shrimp. Techniques allowing detection of viral diseases have led to the reduction of losses in ... Through the use of molecular markers, analysing and identifying fragments of cassava DNA linked to the traits of interest, ... Farmers were given kits for collecting and preserving DNA. Since 2002, efforts to support shrimp farmers in disease control and ...
  http://www.downtoearth.org.in/coverage/agricultural-biotechnolgies-a-boon-to-family-farmers-52862
*  Squamous Cell Carcinoma of the Tonsils | Clinical Cancer Research
... and the limited amount of viral DNA in this control group required Southern blot of PCR product for identification. Less ... viral-free DNA and negative controls (PCR reagents containing no DNA) were included in each PCR amplification. To eliminate ... When HPV is associated with a tumor in a noncausal role, the viral DNA is not strictly preserved in metastases. HPV 12 is ... All negative controls and nonhuman, viral-free DNA samples were negative for β-Globin, HPV, and HLA-DRB1 assay. Positive ...
  http://clincancerres.aacrjournals.org/content/8/4/1093.full
*  Liver Function Tests | eMedical Help
One test measures the 'viral load,' that is, the amount of viral DNA in the blood. Doctors will recommend treatment if there ... Viral Dna, viral load, Viral Loads, what is the treatment of hepatitis b on June 3, 2012. by Dr.Sobia Adnan. ... Tests like the viral load or liver function tests are used to evaluate if medicines are working. People who have large amounts ... Up to one-third of people with very high viral loads (more than one million viral copies per milliliter of blood) will develop ...
  http://emedical-help.com/tag/liver-function-tests/