*  Interactive Fly, Drosophila
Rpd3p also appears to activate telomeric genes repressed by the silent information regulator (SIR) proteins directly, possibly ... amino acid biosynthesis, and carbohydrate transport and utilization, respectively (Bernstein, 2000). Silent information ... In Saccharomyces cerevisiae, heterochromatin-like regions are found near telomeres and at the silent mating-type loci, where ... silent information regulator) protein complex to the chromosome, which occurs at least in part through its association with the ...
  http://www.sdbonline.org/sites/fly/polycomb/sir2-2.htm
*  Ty5 retrotransposon - Wikipedia
2002). Sir4p is one of four 'Silent Information Regulator' proteins that also include Sir1p, Sir2p and Sir3p. Of these, Sir2p, ... Gai X, Voytas DF (June 1998). "A single amino acid change in the yeast retrotransposon Ty5 abolishes targeting to silent ... The Ty5 is a type of retrotransposon native to the Saccharomyces cerevisiae organism. Ty5 is one of five endogenous ... "Silent information regulator 2 family of NAD- dependent histone/protein deacetylases generates a unique product, 1-O-acetyl-ADP ...
  https://en.wikipedia.org/wiki/Ty5_retrotransposon
*  The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent...
To generate fusion proteins of GFP with wild-type hSIRT3 or with amino acids 26-399 of hSIRT3, corresponding coding sequences ... The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev. 13: ... Silent information regulator 2 family of NAD- dependent histone/protein deacetylases generates a unique product, 1-O-acetyl-ADP ... The yeast silent information regulator (Sir)2 protein links cellular metabolism and transcriptional silencing through its ...
  http://jcb.rupress.org/content/158/4/647
*  PMPCB - Wikipedia
The precursor protein contains a 45 amino acid N-terminal fragment as mitochondrion targeting sequence. After cleavage, the ... Schwer B, North BJ, Frye RA, Ott M, Verdin E (Aug 2002). "The human silent information regulator (Sir)2 homologue hSIRT3 is a ... peptidase and the yeast frataxin homolog together maintain mitochondrial iron homeostasis in Saccharomyces cerevisiae". Human ... The Mitochondrial-processing peptidase subunit beta precursor protein is 54.4 KDa in size and composed of 489 amino acids. ...
  https://en.wikipedia.org/wiki/PMPCB
*  Sir3 N-terminal acetylation stabilizes the interaction | Open-i
Silent Information Regulator Proteins, Saccharomyces cerevisiae/chemistry*/genetics/metabolism*. Minor. *Acetylation. * ... 2c). The presence of several acidic amino acids in loop3 suggests that the side chain of H3 Lys79 can assume different ... 2c). The presence of several acidic amino acids in loop3 suggests that the side chain of H3 Lys79 can assume different ... The crystal structure of the N-terminally acetylated BAH domain of Saccharomyces cerevisiae Sir3 bound to the nucleosome core ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC3818696_emss-53785-f0002&req=4
*  Protocols and Video Articles Authored by John D. Aitchison (Translated to Dutch)
Kwantitatieve Phosphoproteomics in Fatty Acid Gestimuleerd Saccharomyces cerevisiae', 'ODELAY: Een grootschalige methode voor ... Saccharomyces cerevisiae Yta7 is a barrier active protein that modulates transcriptional states at the silent mating locus, HMR ... Full incorporation of isotopically heavy amino acids facilitates accurate quantification of proteins from different cultures, ... genome-wide protein-DNA interaction data, database information, and computational predictions of protein-DNA and protein- ...
  https://www.jove.com/author/John+D._Aitchison?language=Dutch
*  Protocols and Video Articles Authored by James Broach (Translated to Turkish)
... cerevisiae yapışma ve Agar Invasion için Testi', 'Canlı İmmobilize Maya Hücreleri Görselleştirme Hızlı Tekniği' ... where its primary function is to recruit silent information regulator (SIR) proteins to establish transcriptional silencing. ... In Saccharomyces cerevisiae, Ras proteins connect nutrient availability to cell growth through regulation of protein kinase A ( ... Six amino acids in the C terminus of Sch9 are directly phosphorylated by TORC1. Phosphorylation of these residues is lost upon ...
  https://www.jove.com/author/James_Broach?language=Turkish
*  Protocols and Video Articles Authored by James Broach (Translated to Hebrew)
Assay עבור הידבקות ואת הפלישה אגר ב cerevisiae ס', 'טכניקה מהירה עבור ויזואליזציה של תאים חיים שמרים ללא יכולת לזוז' ... where its primary function is to recruit silent information regulator (SIR) proteins to establish transcriptional silencing. ... In Saccharomyces cerevisiae, Ras proteins connect nutrient availability to cell growth through regulation of protein kinase A ( ... Six amino acids in the C terminus of Sch9 are directly phosphorylated by TORC1. Phosphorylation of these residues is lost upon ...
  https://www.jove.com/author/James_Broach?language=Hebrew
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Membrane Proteins, Micelles, Molecular Motor Proteins, life sciences, biochemistry, Amino Acids, Peptides, and Proteins, lipid- ... The yeast Saccharomyces cerevisiae has been pivotal in this task. To specifically label and analyze at the ultrastructural ... Each technique provides distinct information that enables spatial and/or temporal resolution, generating data both at single ... Cystic fibrosis transmembrane conductance regulator (CFTR), a chloride channel located primarily at the apical membranes of ...
  https://www.jove.com/visualize/abstract/22412896/involvement-complexin-2-docking-locking-unlocking-different-snare
*  Candida albicans - Wikipedia
In the 3153A strain, a gene called SIR2 (for silent information regulator), which seems to be important for phenotypic ... albicans protein-protein interactions in the model organism S. cerevisiae. To overcome this problem a C. albicans specific two- ... Candidalysin is a cytolytic 31-amino acid α-helical peptide toxin that is released during hyphal formation. It contributes to ... Due to the aberrant codon usage of C. albicans it is less feasible to use the common host organism (Saccharomyces cerevisiae) ...
  https://en.wikipedia.org/wiki/Candida_albicans
*  Protocols and Video Articles Authored by John D. Aitchison (Translated to Arabic)
Saccharomyces cerevisiae Yta7 is a barrier active protein that modulates transcriptional states at the silent mating locus, HMR ... Full incorporation of isotopically heavy amino acids facilitates accurate quantification of proteins from different cultures, ... genome-wide protein-DNA interaction data, database information, and computational predictions of protein-DNA and protein- ... FOXO3 was identified as a negative regulator of Irf7 transcription and we have further demonstrated that FOXO3, IRF7 and IFN-I ...
  https://www.jove.com/author/John+D._Aitchison?language=Arabic
*  Direct evidence for SIR2 modulation of chromatin structure in yeast rDNA | The EMBO Journal
Grenson M, Mousset M, Wiame JM and Bechet J (1966) Multiplicity of amino acid permeases in Saccharomyces cerevisiae IV: ... the nucleolus as a compartment for silent information regulators. EMBO J, 16, 3243-3255. ... Hoffmann W (1985) Molecular characterization of the CAN1 locus in Saccharomyces cerevisiae. A transmembrane protein without N- ... Klar AJS, Fogel S and MacLeod K (1979) MAR1 - a regulator of HMa and HMα loci in Saccharomyces cerevisiae. Genetics, 93, 37-50. ...
  http://emboj.embopress.org/content/16/21/6495
*  Regulation of Organelle and Cell Compartment Signaling: Cell Signaling Collection Volume 3: Edited By: Ralph A Bradshaw and ...
290 Silent Chromatin Formation and Regulation in the Yeast Saccharomyces cerevisiae 291 Gene Silencing and Chromatin ... 274 Translational Control by Amino Acids and Energy 275 Translation Control and Insulin Signaling 276 ER and oxidative stress: ... 268 The Heat-Shock Response and the Stress of Misfolded Proteins 269 Hypoxia-Mediated Signaling Pathways 270 Regulation of mRNA ... 265 The p53 Master Regulator and Rules of Engagement with Target Sequences 266 Nuclear and Cytoplasmic Functions of Abl ...
  https://www.nhbs.com/regulation-of-organelle-and-cell-compartment-signaling-book
*  Negative regulation of the deacetylase SIRT1 by DBC1
Antitumor activity of a small-molecule inhibitor of human silent information regulator 2 enzymes. Cancer Res. 2006;66:4368-4377 ... amino acids 55-61) and nucleotides 2097-2115 (amino acids 560-565) of DBC1, respectively, by using Lipofectamine2000 according ... Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature. 2003;425:191-196. [PubMed] ... Five microlitres of 35S-labelled protein was incubated with 3 μg of the purified GST protein fragments as indicated in the ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2866287/?lang=en-ca
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein Expressed in Saccharomyces cerevisiae ... amino acids, and help assemble iron-sulfur clusters in proteins. Mitochondria also have an essential role in heat production. ... The assay delivers the following information: (i) how a particular treatment (a mutation, a knock-down, an overexpression or a ... Being in essence transcriptionally and translationally silent, these motile cells rely profoundly on diverse signaling ...
  https://www.jove.com/visualize/abstract/21647419/characterization-novel-star-steroidogenic-acute-regulatory-protein
*  Localization of Sir2p: the nucleolus as a compartment for silent information regulators | The EMBO Journal
1996). An antibody against a Sir2p-GST fusion protein (amino acids 275-562) was produced using standard methods (Harlow and ... Sir3 and Sir4 proteins in wild‐type Saccharomyces cerevisiae. J Cell Biol, 134, 1349-1363. ... These include repressor activator protein 1 (Rap1), the silent information regulators (Sir) 2-4 and the N‐termini of histones ... amino acid identity overall with Sir2p, and nearly 80% amino acid identity in the conserved C‐terminal two‐thirds (Brachmann et ...
  http://emboj.embopress.org/content/16/11/3243
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Amino acid labeling, SILAC, Isotope-coded affinity tag, Isotope labeling, Quantitation, Saccharomyces cerevisiae, ER ... can provide important information for designing the next generation multifunctional biomaterials based on different protein ... Our recent studies have led to the isolation of PGRN as an important regulator of cartilage development and degradation 8-11. ... We illustrate here the setting up of ChroP for the enrichment and analysis of transcriptionally silent heterochromatic regions ...
  https://www.jove.com/visualize/abstract/21738675/towards-rigorous-network-protein-protein-interactions-model-sulfate
*  A long noncoding RNA promotes cellulase expression in Trichoderma reesei | Springer for Research & Development
For each of the 64 codons potentially making up a protein the following information is listed: base triplet; the encoded amino ... Martens JA, Laprade L, Winston F. Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene. ... a protein BLAST of the deduced amino acid sequence as well as a nucleotide BLAST of the hax1 gene to the pool of coding ... Other proteins functioning as regulators of PBDE expression, are Ace1 [25], Ace2 [26], Ace3 [27], Cre2 [28], PAC1 [29], and the ...
  https://rd.springer.com/article/10.1186/s13068-018-1081-4
*  Altering nuclear pore complex function impacts longevity and mitochondrial function in S. cerevisiae | JCB
Localization of Sir2p: the nucleolus as a compartment for silent information regulators. EMBO J. 16:3243-3255. doi:10.1093/ ... Depletion of Saccharomyces cerevisiae ribosomal protein L16 causes a decrease in 60S ribosomal subunits and formation of half- ... Yeast were grown in synthetic complete (SC) media without appropriate amino acids supplemented with 2% dextrose or YPD (1% ... Nuclear protein import, but not mRNA export, is defective in all Saccharomyces cerevisiae mutants that produce temperature- ...
  http://jcb.rupress.org/content/208/6/729
*  N-alpha-acetyltransferase 10 - Wikipedia
Van Damme P, Støve SI, Glomnes N, Gevaert K, Arnesen T (August 2014). "A Saccharomyces cerevisiae model reveals in vivo ... transcripts and proteins". Nucleic Acids Research. 35 (Database issue): D61-5. doi:10.1093/nar/gkl842. PMC 1716718 . PMID ... "Loss of amino-terminal acetylation suppresses a prion phenotype by modulating global protein folding". Nature Communications. 5 ... "The yeast ARD1 gene product is required for repression of cryptic mating-type information at the HML locus". Molecular and ...
  https://en.wikipedia.org/wiki/N-alpha-acetyltransferase_10
*  The chronological life span of Saccharomyces cerevisiae - Fabrizio - 2003 - Aging Cell - Wiley Online Library
The silent information regulator proteins Sir2, Sir3, Sir4, which control chromatin silencing at different DNA sites, can ... We are also currently testing the effect of the lack of amino acid biosynthetic enzymes and of the depletion of amino acids on ... of Amino Acids Extends Chronological Lifespan by Suppressing Hyper-Acidification of the Environment in Saccharomyces cerevisiae ... Novel sensing mechanisms and targets for the cAMP-protein kinase A pathway in the yeast Saccharomyces cerevisiae. Mol. ...
  http://onlinelibrary.wiley.com/doi/10.1046/j.1474-9728.2003.00033.x/full
*  Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing | PNAS
1995) Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem ... Deletion of the silent information regulator SIR2 was included as a positive control. Silencing of URA3 renders yeast resistant ... We previously reported that the Saccharomyces cerevisiae protein Asr1 is a RING (really interesting new gene) finger Ub-ligase ... 2004) Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21(8):661-670. ...
  http://www.pnas.org/content/113/5/1309
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Membrane Proteins, Micelles, Molecular Motor Proteins, life sciences, biochemistry, Amino Acids, Peptides, and Proteins, lipid- ... The yeast Saccharomyces cerevisiae has been pivotal in this task. To specifically label and analyze at the ultrastructural ... Proteins of the Rho family of small GTPases are central regulators of the cytoskeleton, and control a large variety of cellular ... Using this technique we were able to obtain important information about the subcellular location of this putative receptor, ...
  https://www.jove.com/visualize/abstract/21423773/the-ubiquitous-dermokine-delta-activates-rab5-function-early
*  Mutation - Wikipedia
Silent mutations are mutations that do not result in a change to the amino acid sequence of a protein but do change the ... Kunz BA, Ramachandran K, Vonarx EJ (April 1998). "DNA sequence analysis of spontaneous mutagenesis in Saccharomyces cerevisiae ... Häsler J, Strub K (November 2006). "Alu elements as regulators of gene expression". Nucleic Acids Research. Oxford University ... Athens, Greece: Information Technology Associates. 30 June 2007. Archived from the original on 24 February 2010. Retrieved 6 ...
  https://en.wikipedia.org/wiki/Mutation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Protein, Protein Binding, Computational Biology, Drug Design, optimization (mathematics), Amino Acids, Peptides, and Proteins, ... This technology allows for the sensitive detection of transient and stable protein interactions using Saccharomyces cerevisiae ... Recently, epigenetic regulators have been discovered as key players in many different diseases 1-3. As a result, these enzymes ... Information on the development of pupal testes and culture conditions are provided alongside microscope imaging data of live ...
  https://www.jove.com/visualize/abstract/23251640/inhibition-n-terminal-lysines-acetylation-transcription-factor