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*  IJMS | Free Full-Text | Antibody Phage Display Libraries: Contributions to Oncology | HTML
Robust techniques combining an antibody library displayed on the phage surface and protein microarray allowed the ... Examples of such antibodies will be discussed here with emphasis on those used as probes for molecular imaging and other ... Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any ... antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different ...
  http://www.mdpi.com/1422-0067/13/5/5420/htm
*  Single-domain antibody - Wikipedia
... single-domain antibodies can be made from common murine or human IgG with four chains. The process is similar, comprising gene ... but often results in a loss of affinity to the antigen. If affinity can be retained, the single-domain antibodies can likewise ... Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic use due to their small size ... camelids antibodies have been shown to be just as specific as a regular antibody and in some cases they are more robust. As ...
  https://en.wikipedia.org/wiki/Single-domain_antibody
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Single-domain antibodies (sdAbs) derived from human V(H) are considered to be less soluble and prone to aggregate which makes ... purification tags that genetically can be fused to the gene of interest are commonly used2 . The most widely used affinity ... Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa1,2, a robust and high-throughput ... of the interaction sites between two proteins as well as screening for peptides that bind the target protein for therapeutic ...
  https://www.jove.com/visualize/abstract/22253912/crystal-structure-human-single-domain-antibody-dimer-formed-through-v
*  anti-CXCR4 Primary Antibodies
Human Single Domain Antibodies That Antagonize Chemokine Receptor CXCR4. in The Journal of biological chemistry 2016 (PubMed) ... The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with ... This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions ... which may hold promise as a target for future therapeutic intervention in septic patients ...
  https://www.antikoerper-online.de/regulation-of-cell-size-pathway-78/cxcr4-antibody-2451/
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Single cell codetection of a gene, its RNA product and cellular regulatory proteins is critical to study gene expression ... "Trypanosoma cruzi Cross Reactive Antibodies" or TcCRA. To validate their cross-reactive nature, these antibodies were affinity- ... of antibodies in blood samples from French human subjects cross-reacting with a synthetic-peptide antigen derived from a ...
  https://www.jove.com/visualize/abstract/24069315/anti-trypanosoma-cruzi-cross-reactive-antibodies-detected-at-high
*  Recombinant immunotoxins with albumin-binding domains have long half-lives and high antitumor activity | PNAS
LMB-170 and LMB-172, with VHH single-domain antibodies from Llama, are also very active. ... and have a high affinity for human or mouse serum albumin. In mice, the RIT containing an ABD from Streptococcus has a longer ... LMB-164 produced regressions of these tumors, but the responses were less robust than for KLM-1 tumors (Fig. 5D). A431/H9 cells ... 2015) Site-specific albumination of a therapeutic protein with multi-subunit to prolong activity in vivo. J Control Release 207 ...
  http://www.pnas.org/content/115/15/E3501
*  Protocols and Video Articles Authored by Kenny Roose
We conclude that single-domain antibodies directed against influenza virus NA protect against H5N1 virus infection, and when ... induced robust M2e-specific serum IgG and protected BALB/c mice against challenge with human and avian influenza A viruses. ... We show here that two M2e-specific monoclonal antibodies with close to identical antigen-binding specificities and affinities ... However, reporter gene-expressing influenza viruses are often attenuated in vivo and may be genetically unstable. Here, we ...
  https://www.jove.com/author/Kenny_Roose
*  Discovery Biology News
... with expertise to develop high affinity single domain antibodies for therapeutic use. ... Inactivation of the Human Papillomavirus E6 or E7 Gene in Cervical Carcinoma Cells by Using a Bacterial CRISPR/Cas RNA-Guided ... Understanding the in vivo profile of anti-fibrotic agents requires robust animal models. GenScript is one of very few biotech ... or as a new bi-specific antibody therapeutic.. Camelid single domain antibodies are a novel bi-specific antibody platform that ...
  https://www.genscript.com/DiscoveryBiology_news.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Affinity-based Isolation of Tagged Nuclei from Drosophila Tissues for Gene Expression Analysis ... Mammalian cell culture is the major platform for commercial production of human vaccines and therapeutic proteins. However, it ... In contrast, vacuum infiltration is more robust and can be scaled-up for commercial manufacture of pharmaceutical proteins. It ...
  https://www.jove.com/visualize/abstract/24751715/development-vhh-antibodies-against-dengue-virus-type-2-ns1-comparison
*  Affibody molecule - Wikipedia
A monoclonal antibody, for comparison, is 150 kDa, and a single-domain antibody, the smallest type of antigen-binding antibody ... "Recombinant human factor VIII-specific affinity ligands selected from phage-displayed combinatorial libraries of protein A". ... These molecules can be used for molecular recognition in diagnostic and therapeutic applications. As with other antibody ... robust proteins engineered to bind to a large number of target proteins or peptides with high affinity, imitating monoclonal ...
  https://en.wikipedia.org/wiki/Affibody_molecule
*  Methods for detecting and treating cancer - The General Hospital Corporation
8(10): 1057-1062, 1995), single domain antibodies, single chain antibodies, and antibodies having multiple valencies (e.g., ... the human antibody proteins on its surface. Genes from the human antibody gene libraries are inserted into a population of ... Nanobodies have the affinity and specificity of conventional antibodies although they are only half of the size of a single ... is that nanobodies can bind therapeutic targets not accessible to conventional antibodies. Recombinant antibody fragments with ...
  http://www.freepatentsonline.com/9885720.html
*  State of play and clinical prospects of antibody gene transfer | Journal of Translational Medicine | Full Text
Therapeutic proof of concept has been demonstrated in mice and non-human primates, and intramuscular vectored mAb therapy is ... Attained serum mAb titers in mice, however, remain far below those obtained with viral vectors, and robust pharmacokinetic data ... RNA presents another emerging platform for antibody gene transfer. Early reports in mice show that mRNA may be able to rival ... To address these issues, antibody gene transfer seeks to administer to patients the mAb-encoding nucleotide sequence, rather ...
  https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1234-4
*  The future of molecular dynamics simulations in drug discovery | Springer for Research & Development
... single-domain proteins (,10 ms simulations of ~105 atoms) will be feasible within 10 years. Folding of large, multi-domain ... It is not so far-fetched to think that intracellular antibody delivery or gene therapy-or some unanticipated, revolutionary ... of macromolecules-and hence their regulatory capacity-far exceeds the limited number of individual human gene products (,25,000 ... The explicit approach may prove more robust in the end. Nature takes advantage of the diffusion of protons through water [74], ...
  https://rd.springer.com/article/10.1007/s10822-011-9517-y
*  Diagnostic and Prognostic Markers for Cancer - Patent application
The term includes polyclonal, monoclonal, chimeric, single domain (Dab) and bispecific antibodies. As used herein, antibody or ... S. Chen, B. Zhu, L. Yu, In silico comparison of gene expression levels in ten human tumor types reveals candidate genes ... The hemochromatosis gene product complexes with the transferrin receptor and lowers its affinity for ligand binding. ... 0172] In additional studies we have identified IHA gene signature having robust and reproducible prognostic value which can be ...
  http://www.patentsencyclopedia.com/app/20130012409
*  Determinants of FIV and HIV Vif sensitivity of feline APOBEC3 restriction factors | Retrovirology | Full Text
In felids such as the domestic cat (Felis catus), APOBEC3 (A3) genes encode for single-domain A3Z2s, A3Z3 and double-domain ... unlike Vif-interacting residues in human A3s, a unique location at the domain interface of Z2 and Z3 and that the linker forms ... and not found in human and mouse A3s. Together, these findings indicate a specific and different A3 evolution in cats and human ... In the Z3 domain we have identified residues involved in binding of FIV Vif, and their mutation blocked Vif-induced A3Z3 ...
  https://retrovirology.biomedcentral.com/articles/10.1186/s12977-016-0274-9
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... Here we present a novel method for assessing the in vitro replication of HIV-1 as influenced by the gag gene isolated from ... Human INO80 complexes consist of 14 protein subunits including Ino80, a SNF2-like ATPase, which serves both as the catalytic ... The widely used (His)6-tag shows affinity for bivalent ions such as nickel or cobalt, which can on their turn be easily coated ...
  https://www.jove.com/visualize/abstract/21297864/heterologous-epitope-scaffold-primeboosting-immuno-focuses-b-cell
*  DiVA - Search result
Single domain affinity proteins for the detection of the genome organizer protein SATB1Manuscript (preprint) (Other academic). ... A gene fusion between Z(basic2) and the acidic human serum albumin binding domain (ABD), derived from streptococcal protein G, ... investigated affibody variants could be completely blocked by premixing the target with the therapeutic monoclonal antibody ... The use of this type of small, robust, and easily expressed anti-idiotypic affibody pair for affinity technology applications, ...
  http://kth.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22personId%22%3A%22authority-person%3A31099+OR+0000-0003-4214-6991%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... The therapeutic effect depended on the dose, affinity and brain and plasma half-life of the VHH construct. Increasing the ... has been proposed as an alternative vector for human gene transfer. The well-characterized biology of CAV2, together with its ... Moreover, this protocol details a robust and reproducible method for assessing the replication capacity of the Gag-MJ4 chimeric ...
  https://www.jove.com/visualize/abstract/25347556/protective-effect-different-anti-rabies-virus-vhh-constructs-against
*  In Vitro Selection of Cancer Cell-Specific Molecular Recognition Elements from Amino Acid Libraries
O. H. Brekke and I. Sandlie, "Therapeutic antibodies for human diseases at the dawn of the twenty-first century," Nature ... F. Fleetwood, N. Devoogdt, M. Pellis et al., "Surface display of a single-domain antibody library on Gram-positive bacteria," ... "Quantitative analysis of the effect of the mutation frequency on the affinity maturation of single chain Fv antibodies," ... This method of separation is robust for large libraries. However, flocculation of the host organisms or inefficient removal of ...
  https://www.hindawi.com/journals/jir/2015/186586/
*  Application # 2017/0205414. DIAGNOSTIC FOR COLORECTAL CANCER - Patents.com
... mini-bodies and single-domain antibodies which are capable of binding an epitopic determinant. Thus, antibodies may exist as ... Other components of the kits will typically include labels, secondary antibodies, substrates (if the gene is an enzyme), ... In a preferred embodiment, the subject is a human. [0101] The "sample" may be of any suitable type and may refer, e.g., to a ... 0061] (ii) administering or recommending a therapeutic for the treatment of colorectal cancer. [0062] In yet another aspect, ...
  http://patents.com/us-20170205414.html
*  Crystal Structure of the Amyloid-β p3 Fragment Provides a Model for Oligomer Formation in Alzheimer's Disease | Journal of...
However, we earlier observed that the 12Y-2 IgNAR single domain antibody crystal structures comfortably accommodated the ... Aβ-IgNAR gene constructs were produced by splice-overlap PCR as previously described (Henderson et al., 2007) using IgNAR 12Y-2 ... 2008) The structure of the amyloid-β peptide high-affinity copper II binding site in Alzheimer disease. Biophys J 95:3447-3456. ... 1C). The interchain association for each dimer (A-C and B-D) is extremely robust, as indicated by an average surface of ...
  http://www.jneurosci.org/content/31/4/1419.long
*  Plus it
However, we earlier observed that the 12Y-2 IgNAR single domain antibody crystal structures comfortably accommodated the ... Aβ-IgNAR gene constructs were produced by splice-overlap PCR as previously described (Henderson et al., 2007) using IgNAR 12Y-2 ... 2008) The structure of the amyloid-β peptide high-affinity copper II binding site in Alzheimer disease. Biophys J 95:3447-3456. ... 1C). The interchain association for each dimer (A-C and B-D) is extremely robust, as indicated by an average surface of ...
  http://www.jneurosci.org/content/31/4/1419
*  Application # 2017/0315132. MULTIPLEXED TOTAL ANTIBODY AND ANTIBODY-CONJUGATED DRUG QUANTIFICATION ASSAY - Patents.com
... and quantify human and humanized antibodies, and their conjugates, that may be present in pre-clinical animal biological ... light chain variable domain of an antibody. In certain embodiments, a single-domain antibody is a human single-domain antibody ... extracellular domain (ECD) antibodies, or anti-idotypic antibodies). The ADC may then be eluted from the affinity capture media ... 0006] Aspects of this disclosure provide robust methods to detect and quantify total antibody and antibody-conjugated drug ...
  http://patents.com/us-20170315132.html
*  COMPOSITIONS AND METHODS OF USE FOR THERAPEUTIC ANTIBODIES - Patent application
... the human antibodies of the invention into nanobody or single domain antibody framework sequences, as described for example in ... The antibody light chain gene and the antibody heavy chain gene can be inserted into separate vector or, more typically, both ... displays a single binding specificity and affinity for a particular epitope. [0033] The term "human antibody", as used herein, ... They are derived from lipocalins, a widespread group of small and robust proteins that are usually involved in the ...
  http://www.patentsencyclopedia.com/app/20120034231
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
However, as another opportunity, these interesting and stable single-domain antibodies8 can be easily engineered with different ... antibacterial agent that is present in many consumer products and has been found to be a therapeutic aid in human allergic skin ... These fragments are readily converted to full-length antibodies and have been validated to exhibit high affinity and ... An amber mutation is first introduced at a specific codon of the gene encoding the protein of interest. The amber mutant is ...
  https://www.jove.com/visualize/abstract/25811612/inhibition-of-type-vi-secretion-by-an-anti-tssm-llama-nanobody