*  Electroacupuncture ST36 prevents postoperative intra-abdominal adhesions formation. | KSparrowMD
Vagal enhancement through electroacupuncture at St 36 prevents post op adhesions by A ST36 significantly decreased angiogenesis ... EA ST36 reduced TNF-α and VEGF levels in adhesive tissue homogenates 7 d after surgery, whereas vagotomy or intraperitoneal ... in the adhesive tissue were evaluated, macroscopic observation and histopathologic evaluation of adhesion formation and ... EA ST36 alleviated the adhesion formation, with both of macroscopic and histopathologic adhesion scores significantly lower ...
  http://www.ksparrowmd.com/electroacupuncture-st36-prevents-postoperative-intra-abdominal-adhesions-formation/
*  Alpha N-catenin, a Neuronal Subtype Catenin Exhibits Fiber Cell Specific Distribution; Regulation of the Alpha N-catenin: N...
... in lens tissue. The expression, distribution and colocalization of αN-catenin with different cell adhesion, cytoskeletal and ... The integrity of N-cadherin and αN-catenin-based cell adhesive interactions in the absence of Prx was determined using Prx null ... Methods : Immunoprecipitation in conjunction with mass spectrometry and immunoblot analysis of lens homogenates was performed ... collectively suggests a crucial role for Prx in formation and stabilization of N-cadherin/αN-catenin-based cell adhesive ...
  http://iovs.arvojournals.org/article.aspx?articleid=2561396
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... Here we present a simple, rapid method for characterizing the adhesive properties of putative homophilic cell adhesion ... Cell-cell adhesion is fundamental to multicellular life and is mediated by a diverse array of cell surface proteins. However, ... The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) ...
  https://www.jove.com/visualize/abstract/20949015/the-dictyostelium-discoideum-acaa-gene-is-transcribed-from
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... method for assessing the adhesive and invasive quality of yeast strains with easy-to-understand steps to isolate the adhesion ... In general, we observed that haploid strains that invade agar are always adhesive, yet not all adhesive strains can invade agar ... Perithecia contain numerous tissue types, produced at specific stages of perithecium development. These include (in order of ...
  https://www.jove.com/visualize/abstract/25390633/high-frequency-rugose-exopolysaccharide-production-vibrio-cholerae
*  Rind (Kuh) Chemokine (C-C Motif) Ligand 2 ELISA Kit
Tissue Homogenates: Rinse 100 mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20 °C. After two ... Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and ... Incubation: To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow ... Serum, Cell Culture Supernatant, Body Fluids, Tissue Homogenate, Plasma. 0.5-10 ng/mL. 0.5 ng/mL. Anmelden zum Anzeigen ...
  http://www.antikoerper-online.de/cellular-response-to-molecule-of-bacterial-origin-pathway-55/ccl2-elisa-kit-4147/cow-elisa-146278/
*  Human TTR ELISA Kit (ABIN366135)
Tissue Homogenates: Rinse 100 mg tissue with 1× PBS, homogenize in 1mL of 1× PBS and store overnight at -20 °C. After two ... Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and ... Incubation: To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow ... For the quantitative determination of human transthyretin (TTR) concentrations in serum, plasma, tissue homogenates. ...
  http://www.antibodies-online.com/kit/366135/Transthyretin+TTR+ELISA+Kit/
*  Neutrophil inhibitors - Patent # 5708141 - PatentGenius
... neutrophil activity including adhesion to vascular endothelial cells are provided. Such compositions may comprise a ... Thus, where adhesion of neutrophils to the endothelium is prevented, the neutrophils will be unable to transmigrate to tissue ... Also such immunoassays may be used in the detection and isolation of Neutrophil Inhibitory Factor from tissue homogenates, ... 88, 1407). Whilemonoclonal antibodies to adhesive proteins have demonstrated the feasibility of using neutrophil adhesion ...
  http://www.patentgenius.com/patent/5708141.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Finally, an in vitro test method is used to evaluate stiffener extraction in an agarose gel model of brain tissue. This ... A unique stiffener design allows for uniform distribution of the PEG adhesive along the length of the probe. Flip-chip bonding ... Electrochemical stripping of PEG-silane layer from ITO microelectrodes allowed for cell adhesion to take place in a spatially ... Raman analysis of the hippocampal homogenates indicates the biopersistence of SWCNT-PEG in the hippocampus 7 days post- ...
  https://www.jove.com/visualize/abstract/26075787/biopersistence-pegylated-carbon-nanotubes-promotes-delayed
*  The WASP and WAVE family proteins | Genome Biology | Full Text
... and WAVE-family proteins act to shape complex tissue architectures. Current efforts are focusing on integrating these pieces of ... the in vivo WAVE complex biochemically purified from tissue homogenates appears to be basically inhibited [40, 68]. Recently, ... In a multicellular context, WAVEs also function in cell-cell adhesion. In cultured epithelial cells, WAVEs localize at the cell ... To construct multicellular organs, the actin cytoskeleton underlying the adhesive junctions that connect neighboring cells must ...
  https://genomebiology.biomedcentral.com/articles/10.1186/gb-2009-10-6-226
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The tissue structure is further disrupted by enzymatic treatment with Collagenase D and DNAse I. The resulting brain homogenate ... where the ability of intercellular adhesion molecule-1 to support binding of pRBC with particular adhesive properties has been ... This allows the sequestration of pRBC in multiple tissues and organs by adhesion to endothelial cells lining the ... Of the ensuing homogenate from each mosquito section, 10 μl was retained while the other 10 μl was transferred to a separate ...
  https://www.jove.com/visualize/abstract/21695207/characterization-two-malaria-parasite-organelle-translation
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
The tissue structure is further disrupted by enzymatic treatment with Collagenase D and DNAse I. The resulting brain homogenate ... where the ability of intercellular adhesion molecule-1 to support binding of pRBC with particular adhesive properties has been ... This allows the sequestration of pRBC in multiple tissues and organs by adhesion to endothelial cells lining the ... The retina is a tissue that lines the interior of the eye and it is the only tissue that allows for a non-invasive analysis of ...
  https://www.jove.com/visualize/abstract/22253852/social-determinants-long-lasting-insecticidal-hammock-use-among-ra
*  Fractalkine (CX3CL1) and Its Receptor CX3CR1 May Contribute to Increased Angiogenesis in Diabetic Placenta
... biological response modifiers and the family of cell adhesion-promoting molecules. ... A sandwich enzyme immunoassay was applied for CX3CL1 measurement in placental tissue homogenates, whereas quantitative ... Chemokine CX3CL1 is unique, possessing the ability to act as a dual agent: chemoattractant and adhesive compound. Acting via ... whereas membrane-anchored form of the chemokine is involved in promotion of leukocyte binding and adhesion. This dual function ...
  https://www.hindawi.com/journals/mi/2013/437576/
*  N-Cadherin Redistribution during Synaptogenesis in Hippocampal Neurons | Journal of Neuroscience
Adhesion and synapses. Recent data strongly link changes in adhesion with alterations in the functional properties of synapses ... Homogenates were prepared from hippocampal neurons that had been grown in culture for 3 weeks by rinsing cells in PBS and then ... 1991) Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul 2:261-270. ... To determine whether the synaptic clustering of cadherins was in fact adhesive as well as to further examine the potential role ...
  http://www.jneurosci.org/content/18/17/6892.full
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thus, the quantitative adhesive capacity of neutrophils from individuals with varying allelic forms of these adhesion molecules ... The SO fed group showed the most severe damage followed by the CO, and FO fed groups that showed the mildest degree of tissue ... Therefore, mice are immunized with CNS homogenates or peptides of myelin proteins. Due to the low immunogenic potential of ... With this method, the neutrophil adhesive capacities from donors with different allelic variants in adhesion receptors can be ...
  https://www.jove.com/visualize/abstract/20454450/evidence-for-stat4-as-common-autoimmune-gene-rs7574865-is-associated
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... T cells accomplish regulated adhesion by controlling the adhesive properties of integrins, a class of cell adhesion molecules ... Lymphocyte adhesion to endothelial cells and tissue extravasation is a multistep process controlled by different adhesion ... However, quantitative PCR from whole tissue homogenate does not allow the assessment of the expression of miRNA in microglia, ...
  https://www.jove.com/visualize/abstract/23951051/epithelial-v-like-antigen-mediates-efficacy-anti-alpha-integrin
*  Patent US5521067 - Bone marrow cell adhesion molecules and process for detecting adherence ... - Google Patents
Also disclosed is a method for detecting binding between cell adhesion membrane proteins and cells having a potential to be ... Wong, H. J., et al., "Cell Adhesion to Low-Mr Proteins Extractable from Mineralized and Soft Connective Tissues," Biochem. J. ... Note that some proteins are more adhesive than others as can be judged by some protein bands appearing weak on protein blot and ... To isolate the protein, the E. coli host cell carrying a recombinant plasmid is propagated, homogenized, and the homogenate is ...
  http://www.google.com/patents/US5521067?dq=7751826
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... Thus, the quantitative adhesive capacity of neutrophils from individuals with varying allelic forms of these adhesion molecules ... With this method, the neutrophil adhesive capacities from donors with different allelic variants in adhesion receptors can be ... The process of neutrophil firm adhesion involves many different adhesion molecules including members of the β2 integrin family ...
  https://www.jove.com/visualize/abstract/24376862/il-17a-induces-endothelial-inflammation-systemic-sclerosis-via-erk
*  Patent US6204030 - Isolation of cellular material under microscopic visualization - Google Patents
... includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue ... During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue ... The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that ... After the transfer surface is activated the transfer surface and tissue sample are separated. ...
  http://www.google.com/patents/US6204030?dq=6125447
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... Thus, the quantitative adhesive capacity of neutrophils from individuals with varying allelic forms of these adhesion molecules ... With this method, the neutrophil adhesive capacities from donors with different allelic variants in adhesion receptors can be ... The future of tissue engineering and cell-based therapies for tissue regeneration will likely rely on our ability to generate ...
  https://www.jove.com/visualize/abstract/25830234/combined-effect-insulin-like-growth-factor-1-cc-chemokine-ligand-2-on
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
... fresh tissue homogenate, non-enzymatic dissociation, primary tissue supernatant ... During the assay, T lymphocytes are allowed to adhere and migrate on a substrate coated with intercellular adhesion molecule-1 ... The migration of T lymphocytes involves the adhesive interaction of cell surface integrins with ligands expressed on other ... An additional benefit of this approach is that the primary tissue supernatant from the homogenates can be used to characterize ...
  https://www.jove.com/visualize/abstract/23555950/an-efficient-low-cost-method-for-gene-transfer-to-t-lymphocytes
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
This allows the sequestration of pRBC in multiple tissues and organs by adhesion to endothelial cells lining the ... where the ability of intercellular adhesion molecule-1 to support binding of pRBC with particular adhesive properties has been ... Of the ensuing homogenate from each mosquito section, 10 μl was retained while the other 10 μl was transferred to a separate ... Tissue formation is crucial for metazoan development and relies on external cues from neighboring tissues. C. elegans is an ...
  https://www.jove.com/visualize/abstract/22479457/subcellular-location-phosphorylation-assembly-into-motor-complex
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... where the ability of intercellular adhesion molecule-1 to support binding of pRBC with particular adhesive properties has been ... The tissue structure is further disrupted by enzymatic treatment with Collagenase D and DNAse I. The resulting brain homogenate ... This allows the sequestration of pRBC in multiple tissues and organs by adhesion to endothelial cells lining the ...
  https://www.jove.com/visualize/abstract/20976232/elevated-cell-specific-microparticles-are-biological-marker-for
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Thereafter, the tissue homogenate was centrifuged in bovine serum albumin to separate vessel fragments from nervous tissue. The ... where the ability of intercellular adhesion molecule-1 to support binding of pRBC with particular adhesive properties has been ... This allows the sequestration of pRBC in multiple tissues and organs by adhesion to endothelial cells lining the ... In tissue engineering, it is desirable to exhibit spatial control of tissue morphology and cell fate in culture on the micron ...
  https://www.jove.com/visualize/abstract/22028878/microenvironmental-geometry-guides-platelet-adhesion-spreading
*  Protein production method - Inter-University Research Institute Corporation Research Organization of Information and Systems
... a culture container for tissue culture (also referred to as a tissue culture or adhesion culture container and the like) and ... In addition, as the adhesive CHO cell, the adhesive CHO-K1 cell before adaptation to suspension culture was used. ... by separating the protein of interest from impurities other than the protein of interest in a culture liquid or cell homogenate ... For the adhesive CHO-K1 cell, medium exchange was carried out when the cell reached confluent on a 6-well plate (2×106 cells), ...
  http://www.freepatentsonline.com/9034649.html
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
T cells accomplish regulated adhesion by controlling the adhesive properties of integrins, a class of cell adhesion molecules ... The tissue structure is further disrupted by enzymatic treatment with Collagenase D and DNAse I. The resulting brain homogenate ... To illustrate the micropipette adhesion frequency method, we show kinetics measurement of intercellular adhesion molecule 1 ( ... The adhesion probability is estimated from the frequency of adhesion events in a sequence of repeated contact cycles between ...
  https://www.jove.com/visualize/abstract/21437282/tcr-affinity-for-self-ligands-influences-development-function