Plating of PBMCs in a tissue culture flask permits adherence of monocytes. Treatment of these monocytes with interleukin 4 (IL- ... The monocytes in turn are formed from stem cells in the bone marrow. Monocyte-derived dendritic cells can be generated in vitro ... Monocytes can be induced to differentiate into dendritic cells by a self-peptide Ep1.B derived from apolipoprotein E. These are ... Mo-DC or MDDC refers to cells matured from monocytes. HP-DC refers to cells derived from hematopoietic progenitor cells. ...
Activation of vascular endothelium, which in turn facilitates the binding of platelets and monocytes ... Activation of platelets to enhance endothelial adherence * ...
Monocytes can also present fungal antigens to elicit adaptive immune responses. These monocyte-mediated pathways may be either ... Monocytes can also present fungal antigens to elicit adaptive immune responses. These monocyte-mediated pathways may be either ... Thus, monocytes are critically involved in mediating the outcomes of these often highly fatal infections. This review will ... Thus, monocytes are critically involved in mediating the outcomes of these often highly fatal infections. This review will ...
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes ... Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC ... To isolate monocytes by the adherence method, culture five times 10 to the seven cells of the resulting PBMC pellet per 10 ... On average, monocytes isolated by the adherence method account for approximately six point two percent of the total peripheral ...
... by monocytes. We studied the role of the interaction of C … ... enters human monocytes through alpha(v)beta(3) integrin and ... Thus, bacterial adherence, but not phagocytosis, is necessary for TNF production by monocytes. The monocyte alpha(v)beta(3) ... We suggest that the adherence of C. burnetii to monocytes via alpha(v)beta(3) integrin enables surface LPS to stimulate TNF ... by monocytes. We studied the role of the interaction of C. burnetii with THP-1 monocytes in TNF production. TNF transcripts and ...
The initiating trigger of this phenomenon is transient adherence of the extracorporeally passaged monocytes to ECP device- ... The ex vivo routing of blood through the ECP device efficiently directs a substantial minority of passaged monocytes to enter ... Recognition of the central role of activated platelets in directing monocytes into the DC maturational pathway, coupled with ... The transcriptome signature of the ECP-processed monocytes is distinctive, marked by the consistent activation of nearly 500 ...
Amlodipine, Atherosclerosis, Atorvastatin, Calcification, Calcium channel inhibitor, Cholesterol, Collagen, Monocyte adherence, ... collagen content and monocyte adherence were quantified using standard histology (aortic root). Raman spectroscopy was used to ... but not amlodipine reduced the adherence of monocytes in the intima. Lesion severity and plaque contents of collagen, ...
Monocytes and macrophages in diabetic individuals are often hyper-responsive to bacterial antigens.65 This up-regulation ... PMN adherence, chemotaxis, and phagocytosis are impaired. Defects in this first line of defense against periodontopathic ... Diabetes results in changes in the function of host defense cells such as polymorphonuclear leukocytes (PMNs), monocytes, and ...
Glycocalyx coverage (stained with WGA-FITC) and calcein-labeled monocyte adherence were measured. In vivo, fundus fluorescein ... Glycocalyx coverage (stained with WGA-FITC) and calcein-labeled monocyte adherence were measured. In vivo, fundus fluorescein ... NaGYY4137 prevented HG-induced loss of BREC glycocalyx, increased monocyte binding to BRECs (p ≤ 0.001), and increased overall ... NaGYY4137 prevented HG-induced loss of BREC glycocalyx, increased monocyte binding to BRECs (p ≤ 0.001), and increased overall ...
... such as recruitment of peripheral monocytes into the parenchyma, adherence of circulating leucocytes to the vascular ...
... and monocytes. For example, human bone marrow MSCs can be isolated by adherence in the Cocoon™ cassette proliferation chamber. ... Isolation of target cell populations by adherence can be applied to most adherent cell types including mesenchymal stem cells ( ...
Addition of hyaluronic acid to the TPBM cultures inhibited bacterial adherence similar to that previously observed for TASM. ... freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. In vitro maturation of TPBM over a ... ACID-DEPENDENT ADHESION OF A SEROGROUP A STRAIN OF PASTEURELLA MULTOCIDA TO CULTURED TURKEY PERIPHERAL BLOOD MONOCYTES Author. ...
Observations from experimental models and autopsy studies in young human subjects suggest that monocyte adherence to the ... The initiation of adhesion increases the expression of selectins, which facilitates the rolling of monocytes, followed by firm ...
Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has ... Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has ... The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. ... The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis. ...
... rolling and stable adherence of the leukocyte with the subsequent extravasation between endothelial cells. On day 1, post-MI, ... Flow cytometric analysis of b classical monocytes (CD45+ CD11b+ Ly6G− NK1.1− Ly6C++ CD43+), c intermediate monocytes (CD45+ ... 9e) monocytes, while intermediate monocytes (Fig. 9d) remained unchanged when compared to sham-operated splenectomized controls ... This was accompanied by higher numbers of Ly6Chi monocytes in peripheral blood, as well as higher expression of monocyte ...
Ab-dependent cellular cytotoxicity by monocytes and granulocytes, and adherence of leukocytes to endothelial cells, fibroblasts ...
Heterodimers of the calcium-binding proteins MRP8 and MRP14 are expressed on the surface of human monocytes upon adherence to ... Monocytes activated by tumour-derived microvesicles from pancreatic, colon and lung cancer cell lines have been found to show ... Eue I, Pietz B, Storck J, Klempt M, Sorg C. Transendothelial migration of 27E10+ human monocytes. Int Immunol 2000;12:1593-1604 ... Zwadlo G, Schlegel R, Sorg C. A monoclonal antibody to a subset of human monocytes found only in the peripheral blood and ...
For this method, PBMCs are cultured in basal medium for 24 h in collagen-coated plates to induce the adherence of hEPCs to the ... Monocytes coexpress endothelial and macrophagocytic lineage markers and form cord-like structures in Matrigel under angiogenic ... Hypoxia inducible factor-1 alpha, endothelial progenitor cells, monocytes, cardiovascular risk, wound healing, cobalt and ... cells in suspension are cultured for 5 days in a second fibronectin-coated plate to induce the adherence of hEPCs to the matrix ...
... inhibits LPS-induced expression of cellular adhesion molecules and adherence of monocytes to human aortic endothelial cells. ... We conclude that LA inhibits LPS-induced monocyte activation and acute inflammatory responses in vitro and in vivo by ... Here we investigated the mechanism by which LA attenuates LPS-induced monocyte activation in vitro and acute inflammatory ... monocyte chemoattractant protein 1, and TNFalpha; and attenuated NF-kappaB activation in lung, heart, and aorta. Lipoic acid ...
RA reduces the oxLDL-induced adherence of THP-1 monocytes onto the endothelial monolayer under HG conditions by downregulating ... Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte ... induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions ... induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions ...
Monocytes were purified by adherence and were allowed to differentiate for 14 days in RPMI 1640 medium supplemented with 5 ng/ ... Human monocyte-derived macrophages were mock-infected, or infected with IDN3006, VN3028IIcl2, or IDN3006/cl2PA at an MOI of 2. ... cell type: monocyte-derived macrophages. treatment: mock. time post-infection: 6 hrs. ...
The expression of TrxR and its regulation by 1,25(OH)_{2} D_{3} and selenite in monocytes might be important for their ... The increase in adherence, ingestion and superoxide anion and TNF\alpha production shown by macrophages from animals with ... Abstract: 1,25(OH)_{2} Vitamin D_{3} (1,25(OH)_{2} D_{3} ) and adhesion propagate monocyte differentiation. We identified the ... The selenoprotein thioredoxin reductase is expressed in peripheral blood monocytes and THP1 human myeloid leukemia cells - ...
For instance, monocyte-derived DCs retained in the skin expressed less CCR7 (CCL21 receptor) than did uninfected monocyte- ... In a previous study, we observed that in vitro co-incubation for 6-24 h with Leishmania decreases the adherence of the J774 ... Hence, the loss of adherence by phagocytes with a high Leishmania burden may impair the exit of these cells from the ... Leon B, Ardavin C: Monocyte migration to inflamed skin and lymph nodes is differentially controlled by L-selectin and PSGL-1. ...
... another potent chemoattractant for neutrophils and monocytes. Following ligation of monocyte C5a receptors, IL-1 is released. ... Adherent receptors mediate adherence of cells and other particles with bound C3b or C4b fragments and are known as CR1 to CR5. ... Adherence reaction mediated through the CR receptors on phagocytes lead to stimulation of phagocytosis, activation of ... Immune complexes bearing C3b are efficiently removed from tissues and from the circulation by monocytes and other phagocytes. ...
Soluble fibrin inhibits monocyte adherence and cytotoxicity against tumor cells: implications for cancer metastasis, John ...
... we show that isolation of monocytes by elutriation is not superior to adherence on plastic in terms of DC yield, viability, or ... Trial patients having undergone monocyte-interfering pharmacotherapy prior to monocyte harvest was associated with an impaired ... Focusing on the isolation of monocytes and the pharmacotherapy preceding monocyte harvest, ... Monocyte-derived dendritic cell (DC)-based anti-cancer immunotherapy represents a promising treatment modality in these ...
Interactions of Neisseria meningitidis with human monocytes. Microb Pathog. 1994;16:153-63. DOIPubMedGoogle Scholar ... Because meningococcal loss of encapsulation enhances adherence to human cells (15,38-44), loss of the capsule might promote N. ... Effect of the (alpha 2-,8)-linked polysialic acid capsule on adherence of Neisseria meningitidis to human mucosal cells. J ... which appeared to increase mucosal surface adherence (15). Moreover, the factor H binding protein (fHbp), which binds to human ...
Observations from experimental models and autopsy studies in young human subjects suggest that monocyte adherence to the ... The initiation of adhesion increases the expression of selectins, which facilitates the rolling of monocytes, followed by firm ...
... monocyte-derived macrophages, neutrophils and lymphocytes). While inflammatory injury drives both fibrogenesis and ... an interaction that seems to be essential for DC recruitment and adherence to the sinusoidal walls and the subsequent MHC I- ... monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). While inflammatory injury drives both fibrogenesis and ... Monocytes have been previously described as perpetuators of inflammation and triggers of fibrotic remodeling [56]. In a CD11c- ...