*  Guanine nucleotide exchange factor - Wikipedia
eIF-2b regenerates the GTP-bound form of eIF-2 for an additional cycle in protein synthesis initiation, i.e., its binding to ... comprises approximately 500 amino acids and was first identified in the CDC25 protein in budding yeast Saccharomyces cerevisiae ... GEFs are multi-domain proteins and interact with other proteins inside the cell through these domains. Adaptor proteins can ... There are 11 identified DOCK family members divided into subfamilies based on their activation of Rac and Cdc42. DOCK family ...
  https://en.wikipedia.org/wiki/Guanine_nucleotide_exchange_factor
*  Center for Theoretical & Mathematical Sciences » People
Carrier Proteins • Case-Control Studies • Cautery • cdc42 GTP-Binding Protein, Saccharomyces cerevisiae • Cell Count • Cell ... Adaptor Proteins, Signal Transducing • Adenosine • Adolescent • Adult • Aedes • Aging • Algorithms • Alleles • Allosteric ... Saccharomyces cerevisiaeSaccharomyces cerevisiae Proteins • Sarcosine • Seasons • Selection, Genetic • Sequence Homology, ... Protein BindingProteins • Proto-Oncogene Proteins • Pupa • Pyrrolidonecarboxylic Acid • Quantitative Trait Loci • ...
  http://fds.duke.edu/db/aas/math/ctms/faculty/hfn
*  Frontiers | Septin-Associated Protein Kinases in the Yeast Saccharomyces cerevisiae | Cell and Developmental Biology
... in studies conducted in budding yeast Saccharomyces cerevisiae is to serve as a scaffold that recruits regulatory proteins, ... in studies conducted in budding yeast Saccharomyces cerevisiae is to serve as a scaffold that recruits regulatory proteins, ... This review summarizes what we currently understand about how the action of septin-associated protein kinases and their ... This review summarizes what we currently understand about how the action of septin-associated protein kinases and their ...
  https://www.frontiersin.org/articles/10.3389/fcell.2016.00119/full
*  Wiskott-Aldrich syndrome protein - Wikipedia
The cooperative binding of CDC42 and PIP2 is thermodynamically favored; binding of one enhances binding of the other. CDC42 and ... Rajmohan R, Meng L, Yu S, Thanabalu T (April 2006). "WASP suppresses the growth defect of Saccharomyces cerevisiae las17Delta ... "The Wiskott-Aldrich syndrome protein-interacting protein (WIP) binds to the adaptor protein Nck". The Journal of Biological ... WASp and N-WASP are analogs, they contain an N-terminal EVH1 domain, a C-terminal VCA domain and central B and GBD (GTP binding ...
  https://en.wikipedia.org/wiki/Wiskott%E2%80%93Aldrich_syndrome_protein
*  NIK is a new Ste20‐related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain...
Friesen H, Lunz R, Doyle S and Segall J (1994) Mutation of Sps1‐encoded protein kinase of Saccharomyces cerevisiae leads to ... The first family of mammalian Ste20‐related kinases are activated by binding GTPbound Cdc42 and Rac and, based on this ... Galisteo ML, Chernoff J, Su Y‐C, Skolnik EY and Schlessinger J (1996) The adaptor protein Nck links receptor tyrosine kinases ... For example, the yeast protein STE5 functions as a scaffolding protein in S.cerevisiae and binds STE20, STE11 and STE7 via non‐ ...
  http://emboj.embopress.org/content/16/6/1279
*  Mbt, a Drosophila PAK protein, combines with Cdc42 to regulate photoreceptor cell morphogenesis | Development
GTP-bound form of Cdc42 binds to Mbt. To verify this result, Cdc42 was expressed as a GST-fusion protein in bacteria and used ... corresponds to the T777A mutation in the Saccharomyces cerevisiae PAK protein Ste20p and has been found to disrupt ... Localisation of Mbt depends on Cdc42. Group II PAKs lack the N-terminal binding site for the Nck/Dock adaptor protein, which ... or GTP-loaded GST-Cdc42 protein. The GST protein and any bound cellular proteins were recovered by precipitation with ...
  http://dev.biologists.org/content/130/3/427
*  SMART: RasGEF domain annotation
To reach the active GTP-bound state, Ras proteins must first release bound GDP. This rate-limiting step in GTP binding is ... seems to be the human homologue of the Saccharomyces cerevisiae cell-division-cycle protein, CDC42Sc. A second S. cerevisiae ... We have shown that concurrent expression of Grb2, but not SH2 or SH3 domain mutants of Grb2, or the alternative adaptor protein ... In COS-1 cells, the DH domain of Sos stimulated guanine nucleotide exchange on Rac but not Cdc42 in vitro and in vivo. The ...
  http://smart.embl-heidelberg.de/smart/do_annotation.pl?DOMAIN=RasGEF&BLAST=DUMMY
*  Protocols and Video Articles Authored by Mark R. Philips
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in ... Live-cell imaging of Jurkat cells expressing green fluorescent protein-RBD, a fluorescent reporter of GTP-bound Ras, revealed ... Icmt is an integral membrane protein restricted to the endoplasmic reticulum (ER). The Saccharomyces cerevisiae ortholog, ... and by adapter proteins [e.g. adhesion and degranulation-promoting adapter protein (ADAP) and Src kinase-associated ...
  https://www.jove.com/author/Mark++R._Philips
*  Here come the septins: novel polymers that coordinate intracellular functions and organization | Journal of Cell Science
In Saccharomyces cerevisiae, septins, a conserved family of GTP-binding proteins, are central to the spatio-temporal ... Alternatively, septins might bind membranes through adaptor proteins such as anillin, which contains an inositol-binding ... to a downstream effector of Cdc42 called Borg3, which contains a Cdc42/Rac-interaction binding (CRIB) motif (Joberty et al., ... Septins are conserved GTP-binding proteins that associate with cellular membranes and the actin and microtubule cytoskeletons. ...
  http://jcs.biologists.org/content/119/1/4
*  Plus it
GTP-bound state and the inactive, GDP-bound state. PAKs have been shown to bind to the GTP-bound form of Cdc42/Rac family ... 2008) Role of the Caenorhabditis elegans Shc adaptor protein in the c-Jun N-terminal kinase signaling pathway. Mol Cell Biol 28 ... The Saccharomyces cerevisiae reporter strain PJ69-4A (James et al., 1996) was cotransformed with plasmids expressing the GAL4 ... One family of Ste20-like kinases, PAK, has been shown to bind the GTP-bound form of the Cdc42/Rac family of GTPases through a ...
  http://www.jneurosci.org/content/36/37/9710
*  List of MeSH codes (D12.644) - Wikipedia
... cdc42 gtp-binding protein MeSH D12.644.360.525.700.050.500 --- cdc42 gtp-binding protein, saccharomyces cerevisiae MeSH D12.644 ... grb2 adaptor protein MeSH D12.644.360.024.298 --- grb7 adaptor protein MeSH D12.644.360.024.300 --- grb10 adaptor protein MeSH ... rab5 gtp-binding proteins MeSH D12.644.360.525.450 --- ral gtp-binding proteins MeSH D12.644.360.525.462 --- ran gtp-binding ... rab gtp-binding proteins MeSH D12.644.360.525.400.025 --- rab1 gtp-binding proteins MeSH D12.644.360.525.400.050 --- rab2 gtp- ...
  https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.644)
*  Biomolecules | Free Full-Text | The TORC2‐Dependent Signaling Network in the Yeast Saccharomyces cerevisiae | HTML
In Saccharomyces cerevisiae, a plasma membrane‐localized protein kinase complex, Target of Rapamicin (TOR) complex‐2 (TORC2) ( ... Pkc1 is also regulated by Pkh1‐ and TORC2‐dependent phosphorylation, but, in addition, by interaction with Rho1‐GTP and lipids ... associated protein kinase Pkh1 (mammalian ortholog is PDK1) and a paralog (Pkh2). For cell survival under various stresses, ... Ypk1 is a central regulator of pathways and processes required for plasma membrane lipid and protein homeostasis, and requires ...
  http://www.mdpi.com/2218-273X/7/3/66/htm
*  Plus it
Here we report that the synprint site can also directly bind the μ subunit of AP-2, an adaptor protein for clathrin-mediated ... 1998) Thermotolerance in Saccharomyces cerevisiae: the yin and yang of trehalose. Trends Biotechnol 16:460-468. ... 2006) Role of numb in dendritic spine development with a Cdc42 GEF intersectin and EphB2. Mol Biol Cell 17:1273-1285. ... 2005) Vesicle endocytosis requires dynamin-dependent GTP hydrolysis at a fast CNS synapse. Science 307:124-127. ...
  http://www.jneurosci.org/content/30/2/655
*  JoVE | Peer Reviewed Scientific Video Journal - Methods and Protocols
Rho proteins are molecular switches that are active in GTP-bound and inactive in GDP-bound state. Their activation is mediated ... The yeast Saccharomyces cerevisiae has been pivotal in this task. To specifically label and analyze at the ultrastructural ... Because the triskelion does not have the ability to bind to the membrane directly, clathrin-binding adaptors are critical to ... the reconstitution of a ceramide-associated complex of recombinant aPKC with the cell polarity-related proteins Par6 and Cdc42 ...
  https://www.jove.com/visualize/abstract/21423773/the-ubiquitous-dermokine-delta-activates-rab5-function-early
*  Interactive Fly, Drosophila
They become activated by interaction with Rho-type small GTP-binding proteins Rac and Cdc42 in the GTP-bound conformation, ... In the yeast Saccharomyces cerevisiae, Ste20 is involved in transmitting the mating-pheromone signal from the betagamma- ... These results suggest a GTP-Cdc42/GTP-Rac triggered multistep activation cascade leading to the stimulation of the adaptor ... PAK1 protein forms a homodimer. The binding of GTP bound Cdc42 or Rac dissociates the PAK dimer, leading to PAK activation. In ...
  http://www.sdbonline.org/sites/fly/cytoskel/pak-kn2.htm
*  C-terminal EH-domain-containing proteins: consensus for a role in endocytic trafficking, EH? | Journal of Cell Science
... the cell fate adaptor protein Numb) bind to human and D. melanogaster EHD proteins (Smith et al., 2004). Syndapin II, a protein ... a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae ... Nucleotide-binding. All mammalian C-terminal EHD proteins contain a putative P-loop motif, an ATP/GTP-binding site found in Ras ... for CDC42 (Hussain et al., 2001) and binding to the Wiscott Aldrich Syndrome protein (WASp) (McGavin et al., 2001). WASp ...
  http://jcs.biologists.org/content/118/18/4093
*  The G Protein-Coupled Receptor Gpr1 Is a Nutrient Sensor That Regulates Pseudohyphal Differentiation in Saccharomyces...
1988 Isolation of a second yeast Saccharomyces cerevisiae gene (GPA2) coding for guanine nucleotide-binding regulatory protein ... signals via the Cdc42/Ste20/mitogen-activated protein kinase module to induce filamentous growth in Saccharomyces cerevisiae. ... and the Gpa2-GTP complex then binds to and activates cAMP production by adenylyl cyclase (see Figure 9). More complex models in ... 2.5 trinocular adaptor (×25 magnification) connected to a Nikon N70 camera. Colonies were photographed directly on solid medium ...
  http://www.genetics.org/content/154/2/609
*  What's hot today: Current papers in developmental biology and gene function
Both genes encode proteins with DNA binding motifs and a conserved protein-protein interaction domain, known as the Broad ... The expression of both isoforms in a Saccharomyces cerevisiae strain lacking the endogenous putative mitochondrial CoA carrier ... CDC-42 activity to the medial tip. Together, these data establish a previously uncharacterized role for polarized CDC-42, in ... adaptor. This report, demonstrates that a newly discovered isoform of Tropomyosin 1, referred to as Tm1C, directly interacts ...
  http://www.sdbonline.org/sites/fly/aimain/model-organisms-december2016.htm
*  Interactive Fly, Drosophila
Ste20p from Saccharomyces cerevisiae is a member of the Ste20/p21-activated protein kinase family of protein kinases. The ... and the GTP-bound form of Cdc42. Evidence is presented that suggests that the hyperphosphorylated form of Cla4 is responsible ... In budding yeast, Swe1 (Wee1 ortholog) is targeted to the bud neck through Hsl1 (Nim1-related kinase) and its adaptor Hsl7, and ... CPEB directly binds to maskin, a protein that can also bind directly to the cap-binding translation initiation factor elF-4E, ...
  http://www.sdbonline.org/sites/fly/newgene/cyclinb2.htm
*  Signaling and Regulation | W.M. Keck Center for Cellular Imaging, U.Va.
Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha in the living pituitary ... Mg2+ deprivation elicits rapid Ca2+ uptake and activates Ca2+/calcineurin signaling in Saccharomyces cerevisiae. Wiesenberger G ... Amyloid precursor protein and Presenilin1 interact with the adaptor GRB2 and modulate ERK 1,2 signaling. Nizzari M, Venezia V, ... Activation of rac and cdc42 video imaged by fluorescent resonance energy transfer-based single-molecule probes in the membrane ...
  http://kcci.virginia.edu/fret-literature/signaling-regulation
*  MAP kinase pathways | Journal of Cell Science
The Pbs2 scaffold joins Ste11 with Hog1 and also links Ste11 to Cdc42-GTP-bound Ste20, through binding of its proline-rich ... All Saccharomyces cerevisiae MAPK pathways were defined genetically. Although components are shared between different pathways ... The scaffold proteins can bind to other proteins, including phosphatases and cytoskeletal proteins. While the significance of ... Ligands bind to cell-surface-receptor tyrosine kinases or G-protein-coupled receptors. This leads to activation of Ras by ...
  http://jcs.biologists.org/content/118/16/3569.full
*  Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase | The EMBO Journal
... a Saccharomyces cerevisiae GTPbinding protein involved in the control of cell polarity. Mol Biol Cell, 4, 1307-1316. ... Members of this protein kinase family bind the Rho‐like small GTP binding proteins Cdc42 or Rac at a conserved binding site ... βγ during pheromone signaling by a mechanism that might involve the adaptor protein Ste5p which binds to Gβ and the protein ... a Saccharomyces cerevisiae gene that encodes a putative GTPbinding protein involved in the control of cell polarity. Mol Cell ...
  http://emboj.embopress.org/content/16/1/83
*  Plus it
... have indicated that shrinkage activation of p38 involves Cdc42 and/or Rac, small GTP-binding proteins (G proteins of the Rho ... That osmotic stress activates MAPKs was first described in Saccharomyces cerevisiae (28) and later also in mammalian cells (67 ... The docking and activation of other receptor adaptor proteins in PDGFR-β-mediated signal transduction in fibroblasts, such as ... A 5-μl aliquot was removed for protein determination using the DC Protein Assay from Bio-Rad. Aliquots of ∼20 μg protein (equal ...
  http://ajpcell.physiology.org/content/294/4/C1046
*  STE20 - Serine/threonine-protein kinase STE20 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - STE20...
Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is ... Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid ... "Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast.". ... "The RA domain of Ste50 adaptor protein is required for delivery of Ste11 to the plasma membrane in the filamentous growth ...
  http://www.uniprot.org/uniprot/Q03497
*  CHN2 Gene - GeneCards | CHIO Protein | CHIO Antibody
Protein Coding), Chimerin 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The ... Regulation of CDC42 activity. GO annotations related to this gene include GTPase activator activity and SH3/SH2 adaptor ... baker's yeast (Saccharomyces cerevisiae). *barley (Hordeum vulgare). *beta proteobacteria (Neisseria meningitidis). *bread mold ... The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). ...
  http://www.genecards.org/cgi-bin/carddisp.pl?gene=CHN2