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*  Protocols and Video Articles Authored by William C. Mobley (Translated to Hindi)
We found that NGF induced a sustained activation of Rap1, a small monomeric GTP-binding protein of the Ras family, and that ... Moreover, NGF-induced activation of Rap1 and sustained activation of MAPK1/2 were markedly suppressed. Inhibition of dynein ... TrkB Binds and Tyrosine-phosphorylates Tiam1, Leading to Activation of Rac1 and Induction of Changes in Cellular Morphology ... Retrolinkin, a membrane protein highly enriched in neuronal endosomes, binds directly to BPAG1n4. Deletion of retrolinkin ...
  https://www.jove.com/author/William+C._Mobley?language=Hindi
*  Epigenetic mechanisms in cognition. - PubMed - NCBI
The ERK cascade is initiated by the activation of Raf kinase via the small GTP-binding protein, ras, or the ras-related protein ... rap-1. Activated Raf then phosphorylates MEK, a dual specific kinase. MEK phosphorylates ERK 1 and 2 on a tyrosine and ... Proteins with methyl binding domains, bind to methylated DNA and associate with other co-factors, such as HDACs or ... and NMDAR activation of Protein Kinase M zeta (PKMzeta) and downstream targeting of NFkappaB signaling in the nucleus. ERK MAPK ...
  https://www.ncbi.nlm.nih.gov/pubmed/21658577?dopt=Abstract
*  THU0021 Defective rap1 activation in synovial fluid t lymphocytes from patients with rheumatoid arthritis | Annals of the...
GTP-bound Rap1 was precipitated using the bacterial expressed fusion protein GST-RalGDS and detected by ECL Western blotting. ... The defective Rap1 activation was not due to increased levels of the Rap1 GTPase activating proteins RapGAP or spa1. While ... This defective activation is not due to increased levels of the Rap1 GTPase activating proteins RapGAP or Spa1. ... Background Rap1 is a small G-protein, member of the Ras GTPase family. Rap1 has been linked to T cell anergy and it is ...
  http://ard.bmj.com/content/60/Suppl_1/A211.3
*  G-protein signaling - RhoA regulation pathway Pathway Map - PrimePCR | Life Science | Bio-Rad
... activity promotes RhoA activation [14], [15]. RAP1, GTP-GDP dissociation stimulator 1 (Rap1GDS1) bound with Kinesin-associated ... G-proteins alpha-q/11 and G-protein alpha-12 family can also associate with LARG thus promoting RhoA activation [5], [6], [7 ... that bind to prenylated GDP-bound Rho proteins and allow translocation between membranes and the cytosol [2], [3]. ... thatpromote exchange of GTP for GDP; GTPase-activating proteins (GAPs) that enhance the intrinsic GTP-hydrolysis activity, ...
  http://www.bio-rad.com/en-us/prime-pcr-assays/pathway/cytoskeleton-remodeling/g-protein-signaling-rhoa-regulation-pathway
*  RAP2A - Wikipedia
"Identification of a specific effector of the small GTP-binding protein Rap2". European Journal of Biochemistry / FEBS. 252 (2 ... "Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon". Oncogene. 21 (53): 8105- ... Ménétrey J, Cherfils J (Nov 1999). "Structure of the small G protein Rap2 in a non-catalytic complex with GTP". Proteins. 37 (3 ... "Identification of a specific effector of the small GTP-binding protein Rap2". European Journal of Biochemistry / FEBS. 252 (2 ...
  https://en.wikipedia.org/wiki/RAP2A
*  The AF-6 Homolog Canoe Acts as a Rap1 Effector During Dorsal Closure of the Drosophila Embryo | Genetics
2003 Cellular functions of the Rap1 GTP-binding protein: a pattern emerges. J. Cell Sci. 116: 435-440. ... Rap1 activation appears to be stimulated by numerous exchange factors that do not act on the prototypic Ras GTPases. Rap1 has ... Rap1 binds Cno in a GTP-dependent manner and partially colocalizes with Cno in vivo: To identify molecules through which Rap1 ... Rap1 belongs to the Ras superfamily of small GTPases, which cycle between an inactive GDP-bound and an active GTP-bound state, ...
  http://www.genetics.org/content/165/1/159
*  Guanine nucleotide binding properties of rap1 purified from human neutrophils | Biochemical Journal
Furthermore, the ability of a cytosolic rap1 GTPase-activating protein to stimulate neutrophil rap1 GTP hydrolysis was not ... These data suggest that the activation of rap in vivo may be regulated by the release of endogenous GDP, but that ... The guanine nucleotide binding properties of rap1 protein purified from human neutrophils were examined using both the protein ... The binding of guanine nucleotides was found to correlate with the loss of endogenous nucleotide from the rap1 protein, which ...
  http://www.biochemj.org/content/267/2/407
*  The signaling module cAMP/Epac/Rap1/PLCε/IP3 mobilizes acrosomal calcium during sperm exocytosis. - PubMed - NCBI
rap1 GTP-Binding Proteins/metabolism. *rap1 GTP-Binding Proteins/physiology. Substances. *Guanine Nucleotide Exchange Factors ... Rab3-GTP, and Rab27-GTP in the acrosomal region; pretreatment with the TAT-cAMP sponge prevented the activation of all three ... The TAT-cAMP sponge consists of the cAMP-binding sites of protein kinase A regulatory subunit RIβ fused to the protein ... Progesterone increased the population of sperm with Rap1-GTP, ... Proteins*BioSystems. *BLAST (Basic Local Alignment Search Tool) ...
  https://www.ncbi.nlm.nih.gov/pubmed/26704387
*  C3G | SpringerLink
Crk SH3 domain binding guanine nucleotide release protein (C3G), Rap guanine nucleotide exchange factor 1 (RAPGEF1), Guanine ... These proteins are inactive when they are bound to GDP and are active when bound to GTP, thereby acting as molecular switches. ... Activation of a Dab1/CrkL/C3G/Rap1 pathway in Reelin-stimulated neurons. Curr Biol. 2004;CB 14:606-10.PubMedCrossRefGoogle ... Conversion of GTPases from inactive GDP bound form to active GTP bound form is performed by GTP exchange factors (GEFs). GEFs ...
  https://link.springer.com/referenceworkentry/10.1007%2F978-3-319-67199-4_101544
*  Cyclic AMP Raises Intracellular Ca2+ in Human Megakaryocytes Independent of Protein Kinase A | Arteriosclerosis, Thrombosis,...
GTP-bound Rap1 to the Rap-binding domain of RalGDS, we investigated the effect of iloprost and forskolin on Rap1 activation. ... bound GTPγS. One of these proteins was identified as Rap1, and GTPγS binding was accompanied by inhibition of Ca2+-ATPases in ... 10 seconds later than the beginning of Rap1 activation, suggesting that Rap1 activation precedes the Ca2+ increase. Rap1 was ... In these cells, iloprost induced only little Rap1 activation, but thrombin induced a 15-fold increase in GTP-Rap1, which ...
  http://atvb.ahajournals.org/content/22/1/179
*  Plus it
Rac is active when bound to GTP and inactive in the GDP-bound state. GTP loading requires the guanine exchange factors (GEF), ... Activation of Rac by cadherin through the c-Src-Rap1-phosphatidylinositol 3-kinase-Vav2 pathway. Oncogene 2006;25:8-19. ... 6C). Downregulation of Rap1a/b further reduced Rac-GTP levels without affecting total Rac protein levels (Fig. 6D), supporting ... Preventing the activation or cycling of the Rap1 GTPase alters adhesion and cytoskeletal dynamics and blocks metastatic ...
  http://cancerres.aacrjournals.org/content/74/18/5256
*  Thermo Scientific™ Pierce™ Active GTPase Pull-Down and Detection Kits
Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti- ... GST fusion protein of specific binding domain, glutathione agarose resin, GTPγS and GDP (100X), lysis-binding-wash buffer, ... Purify GTPases and assay activation levels using specific GST-tagged protein-binding domains (PBD), GSH-agarose beads and anti- ... Kits specific for Arf1, Arf6, Cdc42, Rac1, RalA, Rap1, Ras, and Rho GTPases are offered. The respective binding domain of the ...
  https://www.fishersci.ca/shop/products/pierce-active-gtpase-pull-down-detection-kits-1/PI16121
*  The GDP Exchange Factor AND-34 Is Expressed in B Cells, Associates With HEF1, and Activates Cdc42 | The Journal of Immunology
Rap-1), and GST-RalBP1 (Ral), respectively. After isolation of the GST-chimeric proteins the GTP-bound GTPases were eluted from ... Activation of Rac1 by a Crk SH3-binding protein, DOCK180. Genes Dev. 12: 3331. ... treatment of WEHI-231 cells with GTP-γ-S demonstrated the ability of the assay to detect endogenous GTP-bound RalA and Rap-1 ( ... Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 18: 578. ...
  http://www.jimmunol.org/content/170/2/969?ijkey=5e163188919689daab370c4d49dd15de6a53e605&keytype2=tf_ipsecsha
*  Platelet-neutrophil interactions under thromboinflammatory conditions
Takai Y, Sasaki T, Matozaki T. Small GTP-binding proteins. Physiol Rev. 2001;81(1):153-208. [PubMed] ... Rap1 is activated by cytosolic Ca2+ and diacylglycerol through PLC activation [170] and controls αMβ2 integrin activation ... receptor interactions stimulate diverse signaling pathways such as activation of G-proteins, phospholipase C, protein and lipid ... Activation of the small GTPase rap1 in human neutrophils. Blood. 1998;92(6):2133-2140. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4480154/
*  EPAC-RAP1 Axis-Mediated Switch in the Response of Primary and Metastatic Melanoma to Cyclic AMP | Molecular Cancer Research
The effect of EPAC inhibition on activation of RAP1 (GTP-bound RAP1) was assessed by pull-down assay using GST-RalGDS-RBD ... RAP1-GTP pull-down assay. Cells were lysed with RIPA buffer with protease and phosphatase inhibitors. Equal amount of protein ( ... Contradictory roles have been assigned to RAP1 in melanoma. For example, although higher activated RAP1 (GTP bound RAP1) levels ... there are only a few reports on the role of RAP1 in melanoma. It was reported that RAP1 activity (RAP1-GTP) is increased in a ...
  http://mcr.aacrjournals.org/content/15/12/1792
*  CD25 and CD69 induction by α4β1 outside-in signalling requires TCR early signalling complex proteins | Biochemical Journal
Ras-GTP and Rap1-GTP levels in the lysates were assayed using specific Ras (Cytoskeleton) and Rap1 (Pierce) Activation kits ... Ras-GTP) from the cell lysates. A GST-fusion protein containing the RBD (Rap1-binding domain) of human Ra1GDS (guanine ... Activation of B-Raf and ERK by the small G-protein Rap1 has been demonstrated in various cell types [27]. Rap1-mediated ... A GST-fusion protein containing the RBD of human Ra1GDS was used to affinity precipitate Rap1-GTP from the cell lysates. Rap1- ...
  http://www.biochemj.org/content/454/1/109
*  Protocols and Video Articles Authored by Mark R. Philips
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in ... Ras is a GTP-binding protein that plays multiple important roles in cell activation, including proliferative and inflammatory ... The PM association of GFP-Rap1 was dependent on GTP binding, and GFP-Rap1 was rapidly up-regulated on this compartment in ... We have developed fluorescent, GFP-based probes capable of selectively binding GTP-bound Ras or Rap1 in living cells. We have ...
  https://www.jove.com/author/Mark++R._Philips
*  Regulation of Rap2 in B lymphocytes - UBC Library Open Collections
Rap activation was assessed using GST-RalGDS to pull down the activated GTP-bound forms of Rap proteins. After SDSP A G E and ... Rap1-GTP 21.5 H Anti-Rap1 Blot Figure 4.4. Effects of RapGapII expression on SDF-1-induced Rapl and Rap2 activation in 2PK-3 ... These GTP-binding proteins cycle between an inactive GDP-bound form or an active GTP-bound form which can bind downstream ... These GTP-binding proteins cycle between an inactive GDP-bound form or an active GTP-bound form which can bind downstream ...
  https://open.library.ubc.ca/cIRcle/collections/ubctheses/831/items/1.0090517
*  Pkp3-Rap1 complex is required for maintaining physical | Open-i
Pkp3-Rap1 complex is required for maintaining physical interaction between Rap1 and E-cad. (A) PLA (red dots) showing which of ... supporting the idea that Pkp3 provides a scaffold for binding and activation of Rap1 required for E-cad association (Figure 7E ... B) Western blot showing the immunoprecipitation (left) and total (right) protein levels of Pkp3, Rap1, E-cad, and Pkp2 for ... supporting the idea that Pkp3 provides a scaffold for binding and activation of Rap1 required for E-cad association (Figure 7E ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC4230782_3749fig7&req=4
*  Krev interaction trapped protein elisa and antibody
Recombinant Protein and Krev interaction trapped protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and ... containing membranes in a GTP-bound RAP1-dependent manner (By similarity).. Below are the list of possible Krev interaction ... and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to ... Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound ...
  https://www.mybiosource.com/protein_family.php?root=krev-interaction-trapped-protein
*  RAS guanyl-releasing protein 2
... and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/ ... and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also ... in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May ... The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 ...
  https://pharos.nih.gov/idg/targets/Q7LDG7
*  Measure Small GTPase Activation | Thermo Fisher Scientific - AU
... selective enrichment and detection of GTP-bound Rac1 GTPase through specific protein interaction with the Pak1 protein-binding ... These kits contain a GST-protein binding-domain (PBD or RBD) fusion that is selective for active Rho, Ras, Rac1, Cdc42, Rap1, ... The respective protein-binding domain (PBD) of these downstream effectors is expressed as a GST-fusion protein (Table 1). When ... Rap1. GST-RalGDS-RBD. Cell proliferation and differentiation. Arf1. GST-GGA3-PBD-PBD. Assembly of coat proteins onto budding ...
  https://www.thermofisher.com/au/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/protein-biology-application-notes/measure-small-gtpase-activation.html
*  RAPGEF2 - Wikipedia
1999). "nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding ... of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation. RAPGEF2 ... RAPGEF2 is a cyclic AMP binding protein. Members of the RAS subfamily of GTPases function in signal transduction as GTP/GDP- ... 2007). "Rap1-PDZ-GEF1 interacts with a neurotrophin receptor at late endosomes, leading to sustained activation of Rap1 and ERK ...
  https://en.wikipedia.org/wiki/RAPGEF2
*  Retrotransposition and mutation events yield Rap1 GTPases with differential signalling capacity | BMC Evolutionary Biology |...
... indicating that their conformations resemble that of active GTP-bound Rap1. We furthermore demonstrate that these three Rap1 ... which encode proteins that differ by only a few amino acids from their parental Rap1 proteins. Markedly, human hRap1B-retro and ... Indeed, all retrogene-encoded GTPases have an increased GTP/GDP binding ratio in vivo, ... where Rap1 isoforms of retrogenic origin are functionally distinct from their parental proteins. ...
  https://0-bmcevolbiol-biomedcentral-com.brum.beds.ac.uk/articles/10.1186/1471-2148-10-55
*  Cannabinoid Transporters
Rap1, a little GTP binding proteins turned on by cAMP excitement of EPAC, modulates acinar cell amylase secretion [5] and it is ... An early on feature of acute pancreatitis is activation of zymogens,. An early on feature of acute pancreatitis is activation ... Extracellular LPS is certainly used in membrane bound Compact disc14 (mCD14) with the actions of LPS binding proteins (LBP), ... Description of binding affinity for macromolecular reputation The binding of two protein may very well be a reversible and ...
  http://www.biogeology.org/category/cannabinoid-transporters/