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*  Recombinant Human RhoA protein (ab91068) | Abcam
Ab91068 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE. Abcam provides free… ... a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. ... Small GTP binding protein Rho A. *Transforming protein Rho A. *Transforming protein RhoA ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...
  http://www.abcam.com/recombinant-human-rhoa-protein-ab91068.html
*  RHOA Mutations Are a Hallmark of Angioimmunoblastic T-cell Lymphoma | Cancer Discovery
... activation-associated binding of wild-type RHOA to the rhotekin effector protein and reduced RHOA-dependent actin stress fiber ... suggesting that they occurred prior to a driving RHOA mutation. GTP binding by the RHOAG17V mutant protein was severely reduced ... protein binding compared with wild-type RHOA, indicating that the G17V mutant protein is inactive and may act in a dominant- ... Targeted RHOA sequencing in an extended AITL cohort identified RHOA G17V mutations in 49 of 72 (68%) AITLs. Similarly, Palomero ...
  http://cancerdiscovery.aacrjournals.org/content/4/3/270.2.full
*  RCSB PDB - Protein Feature View - Rhophilin-2 - Q8BWR8 (RHPN2 MOUSE)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Binds specifically to GTP-Rho. May function in a Rho pathway to limit stress fiber formation and/or increase the turnover of F- ... actin structures in the absence of high levels of RhoA activity. UniProt ... Interacts with GTP-bound RhoA and RhoB. Interacts with both GTP- and GDP-bound RhoA. Interacts with KRT18. UniProt ...
  http://www.rcsb.org/pdb/protein/Q8BWR8
*  RhoG regulates endothelial apical cup assembly downstream from ICAM1 engagement and is involved in leukocyte trans-endothelial...
These proteins function as molecular switches and cycle between an inactive GDP-bound state and an active GTP-bound state. ... 1999; Carman and Springer, 2004). Cross-linking of ICAM1 stimulates the assembly of actin stress fibers (Wojciak-Stothard et al ... two sets of DNA oligonucleotides were designed to target human RhoA mRNA and were named RhoA#1 and RhoA#2: ... We made use of the RhoG downstream effector ELMO (engulfment and cell motility), which specifically binds GTP-bound RhoG (Katoh ...
  http://jcb.rupress.org/content/178/7/1279
*  Plus it
ROKα is a p150 serine/threonine kinase binding RhoA only in its active GTP-bound state, promoting the formation of stress ... The GTPase RhoA has been implicated in various cellular activities, including the formation of stress fibers, motility, and ... Protein samples from normal B cells labeled with Cy5 were mixed with protein samples from WM B cells labeled with Cy3 protein ... Actin was used as loading control. D, immunoblotting for Rab4, p62DOK, and HSP90 in another set of WM samples (n = 3) and ...
  http://cancerres.aacrjournals.org/content/67/8/3777
*  Clostridium difficile toxin A - Wikipedia
RhoA regulates the actin cytoskeleton and forms stress fibers and focal adhesions. When RhoA is inactivated via TcdA, its ... Just I, Selzer J, von Eichel-Streiber C, Aktories K (March 1995). "The low molecular mass GTP-binding protein Rho is affected ... These target molecules include RhoA, Rac, and Cdc42, which are regulatory proteins of the eukaryotic actin cytoskeleton and ... The glucosylation prevents proper binding of GTP and blocks activation. TcdA acts preferentially on the GDP-bound form of the ...
  https://en.wikipedia.org/wiki/Clostridium_difficile_toxin_A
*  Simvastatin inhibits the expression of stemness‑related genes and the metastatic invasion of human cancer cells via destruction...
... such as RhoA, a small guanosine triphosphate (GTP)-binding protein. In particular, simvastatin selectively blocks RhoA ... It has also been shown that RhoA controls the formation of stress fibers, while Cdc42 and Rac proteins influence the production ... Many factors promote actin reorganization and cell motility, including small GTPases, such as RhoA, Rho-associated protein ... These proteins cycle between active and inactive states by binding to GTP or GDP, respectively. With regard to the possible ...
  https://www.spandidos-publications.com/ijo/51/6/1851
*  Protocols and Video Articles Authored by Peter D. Burbelo (Translated to Portuguese)
... containing the N-terminal RhoA-binding domain but lacking other regions suggested that the disassembly of F-actin stress fibers ... Rho GTPase activating protein) domain usually function to catalyze the hydrolysis of GTP that is bound to Rho, Rac and/or Cdc42 ... The RhoA-binding Protein, Rhophilin-2, Regulates Actin Cytoskeleton Organization The Journal of Biological Chemistry. Nov, 2002 ... levels of RhoA-GTP suggesting that the Rhophilin-2-induced disassembly of stress fibers was not mediated by decreased RhoA ...
  https://www.jove.com/author/Peter+D._Burbelo?language=Portuguese
*  The emerging role of Slit-Robo pathway in gastric and other gastro intestinal cancers | BMC Cancer | Full Text
GTP-bound RhoA promotes the formation of actin stress fibers and focal adhesions and mediated cell repulsion. However, GTP- ... are key regulators of actin cytoskeletal dynamics [42]. These proteins switch from an inactive GDP-bound state to an active GTP ... Actin polymerization is regulated by several adaptor proteins that can bind to the cytoplasmic motifs of the ROBO receptors. In ... the binding of SLIT to the ROBO receptor leads to reorganization of the actin cytoskeleton. The binding of the SLITs modified ...
  https://bmccancer.biomedcentral.com/articles/10.1186/s12885-015-1984-4
*  RCSB PDB - Protein Feature View - Transforming protein RhoA - P61589 (RHOA RAT)
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. ... via active GTP- or inactive GDP-bound forms); this interaction is direct, blocks the loading of GTP to RHOA and decreases upon ... GTP-bound form) with KCNA2 (via cytoplasmic N-terminal domain) (PubMed:9635436). Interacts (via GTP-bound form) with RIPOR1 ( ...
  http://www.rcsb.org/pdb/protein/P61589
*  RHOA - Wikipedia
Though RhoA is most notably recognized from its unique contributions in actin-myosin contractility and stress fiber formation, ... Cachero TG, Morielli AD, Peralta EG (June 1998). "The small GTP-binding protein RhoA regulates a delayed rectifier potassium ... RhoA contains both inactive GDP-bound and active GTP-bound conformational states; these states alternate between the active and ... Signaling proteins like RhoA, regulate polymerization of actin. Due to differences proteins exhibited between normal and ...
  https://en.wikipedia.org/wiki/RHOA
*  Activated Gα13 Impairs Cell Invasiveness through p190RhoGAP-Mediated Inhibition of RhoA Activity | Cancer Research
... which stimulate exchange of bound GDP by GTP, and inhibited by GTPase-activating proteins (GAP), which promote GTP hydrolysis ... 5B), which correlated with inefficient actin stress fiber formation on Matrigel, as well as with inhibition in the generation ... After elution of bound proteins, they were subjected to Western blotting with anti-RhoA or anti-Rac1 antibodies. ... Vectors coding for GFP-fused forms of wild-type RhoA and Rac1, DN N19-RhoA, and activated V14-RhoA and V12-Rac1 were gifts from ...
  http://cancerres.aacrjournals.org/content/68/20/8221
*  Platelet-neutrophil interactions under thromboinflammatory conditions
... whereas RhoA mediates actin stress fiber formation. In platelets, studies using mice lacking Rac1, Cdc42, or both demonstrated ... Takai Y, Sasaki T, Matozaki T. Small GTP-binding proteins. Physiol Rev. 2001;81(1):153-208. [PubMed] ... Libersan D, Merhi Y. Platelet P-selectin expression: requirement for protein kinase C, but not protein tyrosine kinase or ... It comprises four transmembrane proteins: GPIbα, GPIbβ, GPIX and GPV (2:2:2:1 ratio). The N-terminal region of GPIbα binds to ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC4480154/
*  MDia1 - Wikipedia
A mouse embryo cDNA library was screened to identify a RhoA-GTP-binding protein using a yeast two-hybrid system. Further it was ... Stress fibers are bundles of about 20 actin filaments linked via non-muscle myosin II. In vitro studies showed that mDia1 ... Profilin as a ubiquitous actin-binding protein is associated with most actin monomers in cells. Interactions between profilin- ... Rho-GTP binds to the GDB domain and disrupts the DAD-DID-interaction thus promoting actin assembly. But this requires high ...
  https://en.wikipedia.org/wiki/MDia1
*  Angiotensin II Signal Transduction Through Small GTP-Binding Proteins | Hypertension
beta-Arrestin 1 and Galphaq/11 coordinately activate RhoA and stress fiber formation following receptor stimulation. J Biol ... 26 Rho has been shown to participate in the formation of focal adhesion and actin stress fibers, and it also mediates the ... Small GTP-binding proteins (G proteins) are monomeric G proteins with a low molecular weight of 20 to 40 kDa. A small G protein ... In VSMCs, several GPCR agonists, including Ang II, stimulate RhoA activity.27 Ang II increases GTP-bound RhoA,27 as well as ...
  http://hyper.ahajournals.org/content/48/4/534
*  ARHGEF18 gene - Genetics Home Reference - NIH
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. ... Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include ... explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell ... The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, ...
  https://ghr.nlm.nih.gov/gene/ARHGEF18
*  DLC1 - Wikipedia
The activity of RhoA regulates the formation of actin stress fibers and focal adhesions - complexes of many proteins located at ... functions to enhance the GTPase activity of the Rho-GTPase proteins RhoA and Cdc42, promoting the hydrolysis of their bound GTP ... its ability to enhance activated GTP-bound Rho-GTPases' (specifically, RhoA and Cdc42) intrinsic ability to convert their GTP ... the termini of actin stress fibers which link the actin cytoskeleton with integrin extracellular matrix receptors. Therefore, ...
  https://en.wikipedia.org/wiki/DLC1
*  News - Citation Spotlight: RhoA Activity Regulated by Src-Mediated Phosphorylation of ARHGAP42
... on tyrosine residue 376 which results in activation of ARHGAP42 which in turn de-activates GTP-bound (active) RhoA and ... correlates with increased cell motility that relies upon dynamic changes in the actin cytoskeleton and focal adhesions, changes ... ARHGAP42 that targets RhoA, among other Rho family GTPases, ... the newly discovered and described GTPase activating protein ( ... ARHGAP42 associates with actin stress fibers and focal adhesions, is auto-inhibited by its N-terminal BAR (Bin/amphiphysin/Rvs ...
  https://www.cytoskeleton.com/blog/rhoa-activity-regulated-by-src-mediated-phosphorylation-of-arhgap42/
*  ROCK1 - Wikipedia
G-protein RhoE binds to the N-terminus of ROCK1 and inhibits its activity by preventing RhoA binding. Small G-proteins, Gem and ... Other functions involve smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, ... When a substrate such as GTP-bound RhoA binds to the Rho-binding region of the coiled-coil domain, the interactions between the ... ROCK1 is activated when bound to the GTP-bound form of RhoA. The human ROCK1 gene is located on human chromosome 18 with ...
  https://en.wikipedia.org/wiki/ROCK1
*  M-RIP - Wikipedia
... putative Rho-specific GDP/GTP exchange factor and a RhoA-binding protein: control of neuronal morphology". J. Cell Biol. 137 (7 ... Surks HK, Riddick N, Ohtani K (2006). "M-RIP targets myosin phosphatase to stress fibers to regulate myosin light chain ... "p116Rip targets myosin phosphatase to the actin cytoskeleton and is essential for RhoA/ROCK-regulated neuritogenesis". Mol. ... putative Rho-specific GDP/GTP exchange factor and a RhoA-binding protein: control of neuronal morphology". J. Cell Biol. 137 (7 ...
  https://en.wikipedia.org/wiki/M-RIP
*  The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in ... Leung T, Chen XQ, Manser E, Lim L. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the ... The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy ... Hall A. Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol. 1994;10:31-54. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC232074/?lang=en-ca
*  Rho-associated protein kinase - Wikipedia
Thus in both cases, ROCK activation by Rho induces the formation of actin stress fibers, actin filament bundles of opposing ... The kinase activity is switched on when Rho-GTP binds to the Rho-binding domain of ROCK, disrupting the autoinhibitory ... Leung T, Chen XQ, Manser E, Lim L (October 1996). "The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is ... glial fibrillaric acidic protein), NF-L (neurofilament L protein) F-actin binding proteins: Adducin, EF-1α (elongation factor, ...
  https://en.wikipedia.org/wiki/Rho-associated_protein_kinase
*  Stress fiber - Wikipedia
"The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth ... RhoA (sometimes referred to as just 'Rho') is responsible for the formation of stress fibers, and its activity in stress fiber ... "The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth ... there are three main types of stress fibers: ventral stress fibers, transverse arcs, and dorsal stress fibers. Ventral stress ...
  https://en.wikipedia.org/wiki/Stress_fiber
*  cMET - Page 2 - How NF-B is activated: the role of the IB kinase (IKK) complex
RhoA, Rac1 and Cdc-42 in C-33A, SiHa and CaSki cell lines (Physique ?(Figure2).2). We found that the GTP-bound active forms of ... morphometric analyses clearly displayed a significant difference in terms of number of F-actin stress fibers, higher in CaSki ... that permit the simultaneous high-throughput analysis of proteins in a single tissue section by means of antibody binding or MS ... to actually interact with the actin severing protein gelsolin (GSN). (C-33A), transfected in order to express the HPV16 E7 ...
  http://cancercurehere.com/?cat=60&paged=2
*  Myosin Light Chain Phosphatase | Circulation Research
RhoA was identified as most likely to activate the affected GTP binding protein. The involvement of RhoA in this effect was ... and formation of focal adhesions and stress fibers seems to involve opposing roles of p21-activated kinase and Rho-kinase. ... Adducin, an actin-binding phosphoprotein that signals focal adhesion, has been demonstrated by Kimura et al31 to be directly ... 8 RhoA is also regulated by Rnd1, a new member of the Rho family.20 Rnd1 is constitutively bound to GTP and able to block Ca2+ ...
  http://circres.ahajournals.org/content/87/3/173