How migratory cells find their way through body tissue | Channels - McGill University
The cellular protein actin is linked to a luminescent protein. This causes glowing dots on the cell surface. These are the ... Visually, the networks look like city hubs (podosomes) connected by road-like spokes, composed of actin cytoskeleton filaments ... focuses on a complex of proteins, known as podosomes, found in the membrane of migratory cells and in certain invasive cancer ... The cellular protein actin is linked to a luminescent protein. This causes glowing dots on the cell surface. These are the ...https://mcgill.ca/channels/news/how-migratory-cells-find-their-way-through-body-tissue-263409
JCI - Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
... and band intensities of indicated proteins normalized to β-actin in each lane were compared using ImageJ software (NIH). ... Resultant bands from 5 independent experiments were detected with a luminescent image analyzer (LAS-1000 mini, Fujifilm), ... C and D) Immunoblot detection of PROX1 protein in siControl-LECs and siTIE2-LECs. Densitometric analysis of the relative level ... E and F) Immunoblot detection of TIE2 downstream signaling proteins ERK, p-ERK, AKT, and p-AKT in hDLECs stimulated with ABTAA ...https://jci.org/articles/view/94668
Actin was used as internal control for loading purposes. Western blot result for AP-2α protein production. G. H1838 cells were ... 2B, top) and enhanced the inhibitory effect of fish oil on cell growth as determined by CellTiter-Glo Luminescent Cell ... Actin was used as internal control for loading purposes. B. Cellular protein was isolated from H1838 cells treated with fish ... Actin was used as internal control for loading purposes. Con, untreated control cells. B. Cellular protein was isolated from ...http://mcr.aacrjournals.org/content/7/1/108
Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells.
IRS-1 protein binds to several oncogene proteins. Oxidative stress and reactive oxygen species (ROS) are involved in the ... and the expression level of each protein, relative to that of actin, was determined. The following antibodies including anti- ... and a luminescent image analyzer LAS-1000 (FUJI FILM). Densitometry analysis of Western blots was conducted using Multi Gauge ... IRS-1 protein binds to several oncogene proteins. Oxidative stress and reactive oxygen species (ROS) are involved in the ...http://www.biomedsearch.com/nih/Insulin-receptor-substrate-1-prevents/22788551.html
Batf2/Irf1 Induces Inflammatory Responses in Classically Activated Macrophages, Lipopolysaccharides, and Mycobacterial...
Ab was incubated with the Dynabeads protein G at 4°C for 2 h. Nuclear protein was prepared from unstimulated and IFN-γ- or LPS- ... The membrane was stripped and reprobed with control Abs against β-actin (Sigma-Aldrich, St. Louis, MO). ... Chemoluminescence was recorded with a Fuji LAS-3000 luminescent analyzer. ... Protein was isolated using NE-PER nuclear and cytoplasmic extraction reagents (Thermo Fisher Scientific, Waltham, MA). Protein ...http://www.jimmunol.org/content/194/12/6035
Adipose Fatty Acid Binding Protein Promotes Saturated Fatty Acid-Induced Macrophage Cell Death through Enhancing Ceramide...
β-Actin (Cell Signaling) was quantified as a loading control. Image Quant TL system was used for relative protein ... 1A, 1B). Using a luminescent cell viability assay, we consistently observed that sFAs, but not uFAs, significantly decreased ... FA binding protein. FATP. FA transport protein. FB1. fumonisin B1. GM-BMM. GM-CSF-induced BMM. iNOS. inducible NO synthase. LA ... FA transport protein (FATP) 1, FATP4, TNF-α, CerS2, CerS5, CerS6. Relative mRNA levels were determined using β-actin or HPRT1 ...http://www.jimmunol.org/content/198/2/798
Pathophysiological Role of VEGF on Retinal Edema and Nonperfused Areas in Mouse Eyes With Retinal Vein Occlusion | IOVS | ARVO...
... and a luminescent image analyzer (LAS-4000; Fuji Film Co. Ltd., Tokyo, Japan). For quantitative analysis, the total protein ... and ICAM-1 with normalization to β-actin (C-E, K-M), phosphorylated Akt (F, N), ERK1/2 (G, O) and eNOS (H, P) with ... and the protein concentration of the supernatant was measured with bovine serum albumin with a bicinchoninic acid protein assay ... AQP4 is a water channel protein and is known to be expressed on retinal Müller cells.31 It has been reported that the ...https://iovs.arvojournals.org/article.aspx?articleid=2704875
Depletion of gut commensal bacteria attenuates intestinal ischemia/reperfusion injury | Gastrointestinal and Liver Physiology
Concentrations of protein extracts were determined by the Bradford protein assay using Coomassie Brilliant Blue. Protein ... A: representative IgM staining in each group (arrows). B: representative Western blots of IgM and actin from each group. C: ... and images were obtained using a Fujifilm LAS-4000 luminescent image analyzer (Fujifilm, Valhalla, NY). The density of each ... Protein extraction and Western blotting.. Tissues from the small intestine were immediately snap frozen after harvest and ...http://ajpgi.physiology.org/content/301/6/G1020.long
Highly active promoters and native secretion signals for protein production during extremely low growth rates in Aspergillus...
Afp mRNA and protein abundance were comparable to luciferase measurements throughout retentostat cultivation, validating the ... Finally, a gene encoding the highly commercially relevant thermal hysteresis protein (THP) was expressed in this system, which ... Firstly, promoters of genes encoding either an antifungal protein (Panafp) or putative hydrophobin (PhfbD) were confirmed as ... In order to assess whether these promoters were suitable to produce heterologous proteins during retentostat cultivation, the ...https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0543-2
Protein concentrations were determined with the Bradford protein assay kit (Bio-Rad). Protein samples (20-30 μg) were separated ... Cell viability was measured by CellTiter-Glo Luminescent Cell Viability Assay (Promega). MDA-MB-231 cells and HMLER cells were ... and actin (Roche). ... B, mRNA and protein levels of FOXQ1, E-cadherin, and Vim from ... 4). These proteins bind to E-box elements in the promoter region of E-cadherin, together with other corepressors, leading to ...http://cancerres.aacrjournals.org/content/71/8/3076
M YOSIN VI : Cellular Functions and Motor... & related info | Mendeley
Science (New York, N.Y.). We present a mechanochemical model for myosin V, a two-headed processive motor protein. We derive the ... Luminescent Proteins: genetics. *Luminescent Proteins: metabolism. *Microfilament Proteins. *Microfilament Proteins: metabolism ... The model explains head coordination by showing that the lead head only binds to actin after the powerstroke in the trail head ... We present a mechanochemical model for myosin V, a two-headed processive motor protein. We derive the properties of a dimer ...https://www.mendeley.com/research-papers/m-yosin-vi-cellular-functions-motor-properties/
Patent US6316267 - Luminescent protein stains an their method of use - Google Patents
... polypeptides and proteins in gels and on solid supports, using neutral or anionic complexes of transition metals. ... The staining of other poly(amino acid) labels, for example actin that is used to identify actin-binding proteins, is readily ... Proteins appear as red to orange luminescent bands on a clear background. Compared to Example 7, this is the preferred method ... Luminescent Protein Staining. US20090131640 *. Nov 20, 2008. May 21, 2009. Bio-Rad Laboratories, Inc.. Photoluminescent metal ...http://www.google.com/patents/US6316267?dq=5179747
A, Western blot analysis of Bcl2 and BAX protein upon treatment of cells with 9-Br-nos for the indicated times. β-Actin was ... The luminescent signal, which is directly proportional to the amount of caspase-3 activity, was measured in a luminescence ... we first analyzed the changes in the levels of proapoptotic protein, BAX, and antiapoptotic protein, Bcl2. Western blot ... A, representative immunoblots of cleaved caspase-3 and cleaved PARP along with the loading control, β-actin. B, quantitation of ...http://mct.aacrjournals.org/content/5/9/2366
iv, Western blot analysis of the Hes1 protein expression and β-actin in REH and MO7e cells after 60 min of treatment with ... Signals were detected by a luminescent image analyzer (LAS-100 Plus, Fujifilm, Tokyo, Japan) and quantified by Image Gauge ( ... protein kinase B or Akt kinase, and protein kinase C; and 100-fold selectivity over ERK1, GSK3β, LCK, protein kinase A, p70S6K ... Western blot analysis of the Hes1 protein expression and β-actin in NB4 cells cocultured with MSCs for the indicated times. ii, ...http://cancerres.aacrjournals.org/content/67/2/684
Small molecule inhibition of group I p21-activated kinases in breast cancer induces apoptosis and potentiates the activity of...
PAK1 dysregulation (copy number gain, mRNA and protein expression) was evaluated in two cohorts of breast cancer tissues (n = ... or PAK1 small interfering RNA oligonucleotides dramatically altered signaling to cytoskeletal-associated proteins, such as ... PAK1 is a serine/threonine kinase downstream of small GTP-binding proteins, Rac1 and Cdc42, and is an integral component of ... involved in actin polymerization through inactivation of cofilin family proteins) and filamin A (a large actin-binding protein ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/s13058-015-0564-5
Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells | The...
Anti-caspase-1 (p10) rabbit polyclonal (sc-514), anti-actin goat polyclonal (sc-1615), anti-Fas-associated death domain protein ... Cell Titer-Glo Luminescent Viability Assay kit was from Promega. EnzChek Caspase-3 assay kit and DEVD-cho were from Invitrogen ... cellular FLICE inhibitory protein. cIAP. cellular inhibitor of apoptosis protein. DC. dendritic cell. Dox. doxorubicin. FADD. ... Fas-associated death domain protein. IAP. inhibitor of apoptosis protein. Nec-1. necrostatin-1. P2X7R. P2X7 receptor. PARP. ...http://www.jimmunol.org/content/191/9/4789.long
The association between U2 genes and CBs was disrupted | Open-i
The association between U2 genes and CBs was disrupted by the expression of a β-actin mutant. TetU2-45 cells were transfected ... 4 B in the presence of wt actin or dn actin. (C) Quantification of the effect of wt and dn YFP-actin on association of the U2 ... 4 B in the presence of wt actin or dn actin. (C) Quantification of the effect of wt and dn YFP-actin on association of the U2 ... To test the effect of actin overexpression on transcription of the U2 array, we transfected with wt or mutant YFP-actin ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2140015_jcb1791095f05&req=4
Lanthanide probes - Wikipedia
LRET was also used to study the interaction of the proteins dystrophin and actin in muscle cells. Dystrophin is present in the ... Turro, C; Fu, PK; Bradley, PM (2003). "Lanthanide ions as luminescent probes of proteins and nucleic acids". Metal ions in ... polyaminocarboxylates as luminescent sensors in time-resolved luminescent (TRL) immunoassays. Optimization of analytical ... The luminescent probe may for instance serve to localize the MRI contrast agent. This has helped to visualize the delivery of ...https://en.wikipedia.org/wiki/Lanthanide_probes
Actin was used as a loading control of cytosolic protein. Nuclear protein extracts were prepared, and cleaved PARP was enhanced ... captured and analyzed by the Las-3000 luminescent Image Analyzer (Fuji Film). ... Actin was used as a loading control for cytosolic protein. B, Cytosolic and nuclear extracts of HCT116 cells were prepared and ... Actin and lamin B were used as loading controls of cytosolic and nuclear protein, respectively. C, Nuclear extracts were ...http://mcr.aacrjournals.org/content/15/2/141
Insulin-like growth factor-2 (IGF2) is essential for fetal advancement aswell as | Role of NK1 and NK2 receptors in mouse...
... and anti β-actin (Sigma). Protein levels were assayed with an ECL advance western blotting detection kit (Amersham Biosciences ... and transmission intensities were quantified with a luminescent image analyzer (LAS-3000 Fuji Film). 2.5 Statistical analysis ... The hexahistidine-Ni2+-NTA system is used in protein refinement extensively, and large ... Isolated proteins were fractionated using 10 %10 % SDS gels and electro-transferred to Immobilon-P membranes (Millipore). The ...http://stopvivisection.info/insulin-like-growth-factor-2-igf2-is-essential-for-fetal-advancement-aswell-as/
IKK-2 inhibitor VIII | Role of NK1 and NK2 receptors in mouse gastric mechanical activity
... and anti β-actin (Sigma). Protein levels were assayed with an ECL advance western blotting detection kit (Amersham Biosciences ... and transmission intensities were quantified with a luminescent image analyzer (LAS-3000 Fuji Film). 2.5 Statistical analysis ... Isolated proteins were fractionated using 10 %10 % SDS gels and electro-transferred to Immobilon-P membranes (Millipore). The ...http://stopvivisection.info/tag/ikk-2-inhibitor-viii/
Cancers | Free Full-Text | Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial...
Protein Detection. Cells were lysed in urea lysis buffer (7 M urea, 0.1 M DTT, 0.05% Triton X-100, 25 mM NaCl, 20 mM Hepes pH ... Primary antibodies against β-actin and Vimentin were purchased from Abcam (Cambridge, UK). Antibodies against β-catenin, E- ... Cytotoxicity was measured using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Madison, USA), which quantifies ... However, previous studies have shown that CSCs express high levels of drug transporters [55,56]; anti-apoptotic proteins [57,58 ...http://www.mdpi.com/2072-6694/3/2/1744/htm
Browse by Author/Supervisor - eTheses Repository
Wright, Kimberley Elizabeth (2015) Engineering of a specific binding site for protein labelling with luminescent lanthanide ... Davies, Amy (2014) Imaging of platelet actin nodule with high spatial and temporal resolution. Ph.D. thesis, University of ... Hammond, Stephen Peter (2009) Imaging of luminescent nanoparticles in flows: development of surface active luminescent ... Osborne, Shani Ann Marie (2017) Luminescent ruthenium nanoprobes for applications in dye sensitized solar cells. Ph.D. thesis, ...http://etheses.bham.ac.uk/view/people/Pikramenou=3AZoe=3A=3A.html
CANCER TREATMENT USING C-TYPE NATRIURETIC PEPTIDE - Patent application
NPR-A protein or preabsorption of the NPRC antibody with NPR-C protein. Monoclonal anti-B-actin antibody (Sigma) was used as a ... a luminescent image analyzer. Specificity was revealed by the presence of a signal in rat lung (positive control) and absence ... NPR-C protein. Monoclonal anti-B-actin antibody was used as a loading control. Decrease in Number of Human Prostate ... Re-probing with Beta-Actin was used as a loading control. Example II Small-Cell Lung Cells This invention is the first ...http://www.patentsencyclopedia.com/app/20090170196
Forty-eight hours after transfection, total RNA and protein were harvested for analysis, and the CellTiter-Glo Luminescent Cell ... and anti-β-actin (Santa Cruz Biotechnology; sc-47778). Membranes were then incubated with HRP-conjugated secondary antibodies ( ... Upregulation of MHC class I molecules by VCX3A/B suggests that VCX3A/B proteins are processed by MHC class I proteins in cells ... A human sex-chromosomal gene family expressed in male germ cells and encoding variably charged proteins. Hum Mol Genet 2000;9: ...http://mcr.aacrjournals.org/content/16/4/623