APEX1 purified MaxPab rabbit polyclonal antibody (D02P) - (H00000328-D02P) - Products - Abnova
DNA-(apurinic or apyrimidinic site) lyase,OTTHUMP00000163988,apurinic/apyrimidinic (abasic) endonuclease,apurinic/apyrimidinic ... Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA ... AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair ... Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in ...http://www.abnova.com/products/products_detail.asp?Catalog_id=H00000328-D02P&SL=
APEX1 Pre-design Chimera RNAi - (H00000328-R03) - Products - Abnova
... multifunctional DNA repair enzyme) 1 (APEX1), transcript variant 3, mRNA. (H00000328-R03) - Products - Abnova ... DNA-(apurinic or apyrimidinic site) lyase,OTTHUMP00000163988,apurinic/apyrimidinic (abasic) endonuclease,apurinic/apyrimidinic ... Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA ... AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair ...http://www.abnova.com/products/products_detail.asp?catalog_id=H00000328-R03
APEX1 - Wikipedia
DNA-(apurinic or apyrimidinic site) lyase is an enzyme that in humans is encoded by the APEX1 gene. Apurinic/apyrimidinic (AP) ... Mol CD, Hosfield DJ, Tainer JA (2000). "Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base ... sites (also called "abasic sites") occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by ... Rothwell DG, Hickson ID (1996). "Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease ...https://en.wikipedia.org/wiki/APEX1
DNA-(apurinic or apyrimidinic site) lyase - Wikipedia
"DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination corrected". Nature. 403 (6768): ... In enzymology, DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5'- ... "Homo sapiens GO:0003906 - DNA-(apurinic or apyrimidinic site) lyase activity". HumanCyc. SRI International. Retrieved March 9, ... Several names for DNA AP lyase include: AP lyase; AP endonuclease class I; endodeoxyribonuclease (apurinic or apyrimidinic); ...https://en.wikipedia.org/wiki/DNA-(apurinic_or_apyrimidinic_site)_lyase
Processing of Damaged DNA Ends for Double-Strand Break Repair in Mammalian Cells
Apurinic/Apyrimidinic Endonucleases. Other than apurinic/apyrimidinic (AP) lyases, human cells contain two known AP ... Ku also has a potent lyase activity that serves to cleave abasic sites near DNA ends . This lyase activity also removes ... Ku binds to DNA ends and recruits DNA-PKcs, the XRCC4/DNA ligase IV complex, and XLF. Synapsis of two DSB ends triggers DNA- ... Ku, a core DSB joining protein, can cleave abasic sites near DNA ends. The downstream processes of patching and ligation are ...https://www.hindawi.com/journals/isrn/2012/345805/
AP site - Wikipedia
... apurinic/apyrimidinic site), also known as an abasic site, is a location in DNA (also in RNA but much less likely) that has ... see DNA structure). In alternative fashion, bifunctional glycosylase-lyases can cleave the AP site, leaving a 5' phosphate ... ISBN 978-1-4496-0091-4. Abasic sites in DNA: repair and biological consequences in Saccharomyces cerevisiae. DNA Repair (Amst ... It has been estimated that under physiological conditions 10,000 apurinic sites and 500 apyrimidinic may be generated in a cell ...https://en.wikipedia.org/wiki/AP_site
I. A. Eliseeva - Semantic Scholar
Bifunctional DNA glycosylases also possess associated apurinic/apyrimidinic (AP) lyase activity that nick the damaged DNA ... strand at an abasic (or AP) site, formed either spontaneously or at the first step. … (More) ... DNA glycosylases are key enzymes in the first step of base excision DNA repair, recognizing DNA damage and catalyzing the ... Effect of the multifunctional proteins RPA, YB-1, and XPC repair factor on AP site cleavage by DNA glycosylase NEIL1. ...https://www.semanticscholar.org/author/I-A-Eliseeva/2223832
Nobel prize in Chemistry - 2015 (Background)
Each day our DNA is damaged by UV radiation, free radicals and other carcinogenic substances, but even without such external ... the apurinic/apyrimidinic (AP) endonuclease, which cleaves the DNA backbone at the 5′ side of the abasic position. The AP ... Verly, W.G., Y. Paquette, and L. Thibodeau, Nuclease for DNA apurinic sites may be involved in the maintenance of DNA in normal ... because the DNA glycosylases concerned possess endogenous AP lyase activity themselves. In a final step, DNA ligase III/XRCC1 ...https://www.slideshare.net/ashokjntu/nobel-prize-in-chemistry-2015-background
Base Excision Repair, AP Endonucleases and DNA Glycosylases
Base excision repair is an important cellular mechanism that protects the integrity of DNA by repairing damage caused by ... Mol CD, Hosfield DJ and Tainer JA (2000) Abasic site recognition by two apurinic/apyrimidinic endonuclease families in DNA base ... 2000) The lyase activity of the DNA repair protein β‐polymerase protects from DNA‐damage‐induced cytotoxicity. Nature 405: 807- ... Cleavage of an abasic site. Products of cleavage by AP endonuclease have 3′‐hydroxyl and 5′‐deoxyribosephosphate termini (lower ...http://www.els.net/WileyCDA/ElsArticle/refId-a0000563.html
DNAtraffic - a database for systems biology of DNA metabolism and repair
Removes damaged bases from DNA, leaving an abasic site.. The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is ... DNA glycosylase/AP lyase Nei*DNA-(apurinic or apyrimidinic site) lyase Nei*Endonuclease VIII ... Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta ... Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes ...https://dnatraffic.ibb.waw.pl/protein/protein_detail/244/
ENGINEERED DEMETER 5-METHYLCYTOSINE DNA GLYOSYLASE WITH IMPROVED YIELD, STABILITY AND SOLUBILITY - Patent application
... helix-hairpin-helix DNA glycosylases have both DNA glycosylase and apurinic/apyrimidinic (AP) lyase activities. The DNA ... creating an abasic site, whereas the lyase activity nicks the DNA. An AP endonuclease generates a 3'-hydroxyl used by a DNA ... A DNA ligase seals the nick to complete the repair process. DNA glycosylase/lyases have not been implicated in mammalian ... apurinic/apyrimidinic (AP) lyase, including conserved Domain A 1Tyr Lys Gly Asp Gly Ala Leu Val Pro Tyr Glu Ser Lys Lys Arg ...http://www.patentsencyclopedia.com/app/20120149023
AP endonuclease - Wikipedia
Apurinic/apyrimidinic (AP) endonuclease is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main ... Class I AP endonucleases (EC 184.108.40.206) cleave 3' to AP sites by a β-lyase mechanism, leaving an unsaturated aldehyde, termed a ... "DNA-bound structures and mutants reveal abasic DNA binding by APE1 DNA repair and coordination". Nature. 403 (6768): 451-456. ... Levin, Joshua D; Demple, Bruce (1990). "Analysis of class II (hydrolytic) and class I (beta-lyase) apurinic/apyrimidinic ...https://en.wikipedia.org/wiki/AP_endonuclease
A general role of the DNA glycosylase Nth1 in the abasic sites cleavage step of base excision repair in Schizosaccharomyces...
... apurinic/apyrimidinic, AP) sites that are both cytotoxic and mutagenic, and must be removed efficiently to maintain genetic ... One of the most frequent lesions formed in cellular DNA are abasic ( ... DNA Mutational Analysis. DNA Repair*. DNA-(Apurinic or Apyrimidinic Site) Lyase / genetics, physiology*. Multienzyme Complexes ... One of the most frequent lesions formed in cellular DNA are abasic (apurinic/apyrimidinic, AP) sites that are both cytotoxic ...http://www.biomedsearch.com/nih/general-role-DNA-glycosylase-Nth1/15452279.html
Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus...
... phosphodiester bonds of the resulting apurinic/apyrimidinic site (AP lyase activity). The crystal structure of MutM from an ... DNA glycosylase activity, which excises various damaged bases from DNA to produce an aldehydic abasic site; (ii) an AP lyase ... A DNA glycosylase which excises imidazole ring‐opened purines and nicks DNA at apurinic/apyrimidinic sites. J Biol Chem, 265, ... DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites. Proc Natl Acad Sci USA, 86, ...http://emboj.embopress.org/content/19/15/3857
Drosophila Larval Brain Neoplasms Present Tumour-Type Dependent Genome Instability | G3: Genes | Genomes | Genetics
"DNA-(apurinic or apyrimidinic site) lyase activity", we hypothesize that the observed increase in C , A transversion may derive ... C , A (G , T) transversions are commonly produced by the formation of apurinic (abasic) sites or 8-hydroxy-2′-deoxyguanosine (8 ... Pair-end DNA sequencing. DNA samples from tumors and their relative controls were processed in parallel. Genomic DNA of each ... Adapter ligation and DNA cluster preparation are performed by Illumina Nextera DNA Sample Preparation KIT (Illumina), and tumor ...http://www.g3journal.org/content/8/4/1205
Strand-specific PCR of UV radiation-damaged genomic DNA revealed an essential role of DNA-PKcs in the transcription-coupled...
... has N-glycosylase and apurinic/apyrimidinic lyase (AP lyase) activities; it can locate and bind to pyrimidine dimers in dsDNA ... which results in an abasic site. Therefore, the genomic DNA with residual pyrimidine dimers can be fragmented into small pieces ... Depletion of DNA-PKcs does not affect the ggNER of UV-induced DNA damage. DNA damage from UV exposure results in the frequent ... Since RNA polymerase II is a substrate of DNA-PKcs , it was suggested that DNA-PK could act as a DNA damage-sensor that ...https://bmcbiochem.biomedcentral.com/articles/10.1186/1471-2091-12-2
The Ogg1 protein of Saccharomyces cerevisiae: a 7,8-dihydro-8-oxoguanine DNA glycosylase/AP lyase whose lysine 241 is a...
... hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites. ... an N-glycosylase/abasic lyase that proceeds via an imino enzyme-DNA intermediate. J Biol Chem. 1995 Oct 6;270(40):23475-23484. ... Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase. ... DNA containing a chemically reduced apurinic site is a high affinity ligand for the E. coli formamidopyrimidine-DNA glycosylase ...http://pubmedcentralcanada.ca/pmcc/articles/PMC146873/
A Novel Inhibitor of DNA Polymerase β Enhances the Ability of Temozolomide to Impair the Growth of Colon Cancer Cells |...
Intragenic suppression of an active site mutation in the human apurinic/apyrimidinic endonuclease. J Mol Biol 1999;287:47-57. ... A. dRP-lyase activity. Top, schematic representation of dRP-lyase DNA substrate and product. Autoradiogram shows the effect of ... Because abasic DNA damage can also be repaired by LP-BER, there is a need for agents that can specifically block both Pol-β- ... B. SMI has no effect on APE1 binding to AP-site DNA. A 32-mer duplex DNA (10 nmol/L) containing a single tetrahydrofuran was ...http://mcr.aacrjournals.org/content/7/12/1973.long
Base excision repair - Wikipedia
"Coordination of Steps in Single-nucleotide Base Excision Repair Mediated by Apurinic/Apyrimidinic Endonuclease 1 and DNA ... Some lesions, such as oxidized or reduced AP sites, are resistant to pol β lyase activity and, therefore, must be processed by ... Atamna H, Cheung I, Ames BN (2000). "A method for detecting abasic sites in living cells: age-dependent changes in base ... MBD4 protein binds preferentially to fully methylated CpG sites and to the altered DNA bases at those sites. These altered ...https://en.wikipedia.org/wiki/Base_excision_repair
Repair of Nucleic Acids for Improved Amplification - Patent application
... apurinic/apyrimidinic (AP) sites, mutagenized nucleotides, modified nucleotides, nicks, gaps and DNA-DNA or DNA-protein cross- ... Abasic sites can be recognized and cleaved by AP endonucleases and/or AP lyases. Class II AP endonucleases cleave at AP sites ... to the cleaved abasic site. It also leaves a deoxyribose-5'-phosphate (dR5P) on the DNA fragment 3' to the cleaved abasic site ... The product of this reaction is an apurinic or apyrimidinic site (AP site) that must be correctly filled. This can be achieved ...http://www.patentsencyclopedia.com/app/20110201056
DNA glycosylase - Wikipedia
... creating an apurinic/apyrimidinic site, commonly referred to as an AP site. This is accomplished by flipping the damaged base ... "Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation". Proc. Natl. Acad. Sci. U.S.A. 103 (31): 11796- ... NEIL1 protein recognizes (targets) and removes certain oxidatively-damaged bases and then incises the abasic site via β,δ ... Uracil-DNA glycosylases are DNA repair enzymes that excise uracil residues from DNA by cleaving the N-glycosydic bond, ...https://en.wikipedia.org/wiki/DNA_glycosylase
DNASU Plasmid | OGG1 (Homo sapiens)...
8-hydroxyguanine DNA glycosylase,AP lyase,DNA-apurinic or apyrimidinic site lyase,N-glycosylase/DNA lyase,OGG1 type 1f ... Removal of DNA patch containing abasic residue. Reactome : Resolution of AP sites via the multiple-nucleotide patch replacement ... Displacement of DNA glycosylase by APE1. Reactome : Recognition and association of DNA glycosylase with site containing an ... Base-Excision Repair, AP Site Formation. Reactome : Base-free sugar-phosphate removal via the single-nucleotide replacement ...http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=433982
REPAIRtoire - a database of DNA repair pathways
DNA-(apurinic or apyrimidinic site) lyase, Apurinic-apyrimidinic endonuclease 1, APEX nuclease, Protein REF-1., Protein SHORT ... DNA-bound structures and mutants reveal abasic DNA binding by APE1 and DNA repair coordination [corrected] Mol CD, Izumi T, ... CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, ... Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease HAP1. Rothwell DG, Hickson ID ...http://repairtoire.genesilico.pl/proteins/47/
A DNA glycosylase which excises imidazole ring-opened purines and nicks DNA at apurinic/apyrimidinic sites. J Biol Chem 1990; ... A major mechanism of directed prereplicative excision of 8-oxodG is the OGG1 glycosylase/apurinic/apyrimidinic (AP) lyase- ... Processing of bistranded abasic DNA clusters in γ-irradiated human hematopoietic cells. Nucleic Acids Res 2004;32:5609-20. ... Base excision repair by NTH1 and hOGG1: a two edged sword in the processing of DNA damage in γ-irradiated human cells. DNA ...http://mct.aacrjournals.org/content/8/7/2015
DNA polymerase β is able to repair breaks in switch regions and plays an inhibitory role during immunoglobulin class switch...
... abasic sites are subsequently recognized by apurinic/apyrimidic endonucleases (APEs), which nick the DNA backbone to create DNA ... 5-phosphate lyase and DNA synthesis activities of mammalian DNA polymerase beta by apurinic/apyrimidinic endonuclease 1. J. ... DNA polymerase lambda protects mouse fibroblasts against oxidative DNA damage and is recruited to sites of DNA damage/repair. J ... DNA polymerase beta promotes recruitment of DNA ligase III alpha-XRCC1 to sites of base excision repair. Biochemistry. 44:10613 ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2118644/?lang=en-ca