Evaluation of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor ... of a Soluble Tetrazolium/Formazan Assay for Cell Growth and Drug Sensitivity in Culture Using Human and Other Tumor Cell Lines1 ... Most human tumor cell lines examined metabolized XTT less efficiently than MTT; however, the addition of phenazine methosulfate ... Drug profiles obtained with representative human tumor cell lines for several standard compounds utilizing the XTT-PMS ...
Cells were seeded at 1 x 105 cells/well in 48-well plates. After incubation, the culture medium was discarded, cells were ... Cells and cell growth conditions. The BT-474 human breast cancer cell line was purchased from American Type Culture Collection ... Cell signal inhibition assays. For cell signal inhibition assays, BT-474 cells were seeded at 1 x 105 cells/well in 48-well ... in Cultured Tumor Cells, Mouse and Rat Xenograft Tumors, and in an HER2-Positive Brain Metastasis Model. Journal of Cancer, 4(7 ...
More recently, tumour-treating fields therapy (TTFields) has demonstrated modest survival benefit and been clinically approved ... glioma stem-like cells (GSCs) in combination with the previously validated preclinical Inovitro™ TTFields system together with ... cells were harvested and re-seeded in matrigel-coated 6-well tissue culture plates at varying densities (300 and 500 cells/well ... A final cell suspension of 1 × 105 cells/mL in PBS for each sample was produced. 12.5 μL of cell suspension was then mixed with ...
Circulating tumour DNA (ctDNA) from the same patient blood sample, was assessed by ddPCR targeting tumour-specific mutations. ... Here we compared two microfluidic devices for the recovery of circulating melanoma cells. The presence of CTCs in 43 blood ... Circulating tumour cells (CTCs) can be assessed through a minimally invasive blood sample with potential utility as a ... Cell culture. The melanoma cell lines A2058 and SK-MEL-5 were used in spiking experiments due to their differential cell size. ...
Short-term cultures of tumour-derived colorectal cancer cells - A novel in vitro model for the evaluation of angiogenesis in ... Short-term cultures of tumour-derived colorectal cancer cells - A novel in vitro model for the evaluation of angiogenesis in ...
The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant ... The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant ... The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant ... The in vitro MDA-MD-231 cell culture results demonstrated that cells cultured on the BC/gelatin scaffolds had significant ...
... activity in inducing tumor cell-specific cytotoxicity and suppressing aggressive tumor phenotypes in human breast cancer cells ... cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in ... All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated ... Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines ( ...
... cells. The mice were divided into five groups: (1) tumor control; (2) lycopene (5 mg/kg); (3) sorafenib (30 mg/kg); (4) ... The mice were divided into five groups: (1) tumor control; (2) lycopene (5 mg/kg); (3) sorafenib (30 mg/kg); (4) lycopene (2 mg ... The results showed that lycopene reduced the number of metastatic tumors in the lungs, which was further suppressed by the ... The results showed that lycopene reduced the number of metastatic tumors in the lungs, which was further suppressed by the ...
... we researched by cytogenetic technique in the peripheral blood lymphocytes cultures and in the tumors cells cultures from ... LATENT PAPILLOMAVIRUS INFECTION AND CHROMOSOMAL INSTABILITY STUDIES IN PERIPHERAL BLOOD LYMPHOCYTES AND TUMORS CELLS CULTURES ... latent papillomavirus infection and chromosomal instability studies in peripheral blood lymphocytes and tumors cells cultures ... but also involved intracellular genome of host cell.. ...
Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma ( ... Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma ( ... Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line ... Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type TP53 and a ccRCC cell line ...
The cells were plated at a density of 3,000 cells/well in 96-well plates with 6 replicates, cultured in DMEM, and the next day ... and cell viability was measured via MTT assay; (c) on migration of 293T cells; 293T cells were transiently transfected with ... and cell viability was measured via MTT assay; (c) on migration of 293T cells; 293T cells were transiently transfected with ... and cell viability was measured via MTT assay; (c) on migration of 293T cells; 293T cells were transiently transfected with ...
Effect of tumor necrosis factor a on steroidogenesis in cultured human ovarian granulosa cells. Putowski, Lechosław T. (1959 ... Effect of tumor necrosis factor a on steroidogenesis in cultured human ovarian granulosa cells ...
Anomalous reactions of mouse alloantisera with cultured tumor cells. II. Cytotoxicity is caused by antibodies to leukemia ... Neoplasm:, Serology: Antigen,, Types of Tumors:, Transplantable Tumors: EL 4, Strains: AKR, C57BL/6, C57BL/10 ...
... especially germ cells. Normal somatic cells, however, usually do not express telomerase. In the malignant tumor, deregulation ... is expressed by malignant tumor and stem cells, ... Tumor Cells, Cultured Substances * Antigens, Nuclear * DNA- ... is expressed by malignant tumor and stem cells, especially germ cells. Normal somatic cells, however, usually do not express ... using the malignant tumors of adenocarcinoma, squamous cell carcinoma, and malignant lymphoma, and somatic tissues of testis, ...
Cornain S, Becker S, Klein E. Sensitization of rat T cells to syngeneic tumor cultures by cocultivation in diffusion chambers. ... Cornain, S, Becker, S & Klein, E 1977, Sensitization of rat T cells to syngeneic tumor cultures by cocultivation in diffusion ... Cornain, S., Becker, S., & Klein, E. (1977). Sensitization of rat T cells to syngeneic tumor cultures by cocultivation in ... Cornain, Santoso ; Becker, S. ; Klein, E. / Sensitization of rat T cells to syngeneic tumor cultures by cocultivation in ...
When tested in normal ovarian surface epithelial and ovarian clear cell carcinoma cell culture models, encapsulation of TPPS in ... cESG significantly improved cell death in response to light treatment compared to free drug alone. Dosages as low as 0.16μM ...
Cell Viability. Normal human keratinocytes were cultured and plated on an 8-well plate (Lab-Tek II Slide, 8 Chamber (Nalge Nunc ... tumor clearance was mediated through cytotoxic CD8+ T cells, arguing for immune-dependent clearance of tumor cells. ... 28 tumors). CWO treated tumors regressed (wk 0=12 tumors vs wk 11=0 tumors). Arrow denotes a malignant conversion in the ... 4. CWO-induced tumor regression requires cytotoxic T cells.. A. Epidermis and tumors treated with CWO or vehicle were analyzed ...
Wright KO, Messing EM, Reeder JE (2004). "DBCCR1 mediates death in cultured bladder tumor cells". Oncogene. 23 (1): 82-90. doi: ... Perforin is stored in granules within cytotoxic T-cells and is responsible for killing virally infected and transformed cells. ... Perforin is released by cytotoxic T cells and lyses virally infected and transformed cells. In addition, perforin permits ... Cell. 121 (2): 247-56. doi:10.1016/j.cell.2005.02.033. PMID 15851031. S2CID 1613454. Huang Y, Qiao F, Abagyan R, Hazard S, ...
3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very ... Culturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular ... 3D patient-derived tumor organoids hold great potential for many basic and translational applications. However, traditional ... Cell / Tissue Culture *Cell Adhesion Assays *Cell Lines, Stem Cells and Primary Cells *Cell-Based Assays *DNA / RNA Extraction ...
Human NK cells engineered to express a PD-L1 chimeric antigen receptor can control murine and human tumors and reduce myeloid ... Tumor cells and culture. Request a detailed protocol A panel of four human papillomavirus (HPV) negative (UM-SCC-1, UM-SCC-11A ... As NK cells detect and eliminate tumor cells via MHC- and antigen-independent mechanisms, NK cell-based immunotherapy may ... cells are binding PD-L1 but not killing the cells yet the blocking of PD-L1 is allowing CD8 T cells to kill the tumor cells, ...
Assessment of the effect of boron neutron capture therapy on tumor cell lines and primary embryonic cell culture. Siberian ... infiltrative malignant tumors, due to the selective destruction of tumor cells without damaging the surrounding normal tissues ... Human cell cultures: glioblastoma (U87), colorectal human adenocarcinoma (SW-620), human melanoma (SK-Mel28) and primary ... Objective: to assess the effect of BNCT on tumor and normal cell lines using borphenylalanine (BPA), borcaptate (BSH) and ...
Patient-derived tumor organoid cultures identify a chemo-resistant sub-population in esophageal squamous cell carcinoma. - ... Patient-derived tumor organoid cultures identify a chemo-resistant sub-population in esophageal squamous cell carcinoma. ... Conference Paper: Patient-derived tumor organoid cultures identify a chemo-resistant sub-population in esophageal squamous cell ... In recent years, patient-derived tumor organoid culture has been developed as an efficient and economic model to mimic and ...
... of Chemotherapeutic Drugs and Heterocyclic Compounds at Application on the Cells of Primary Culture of Neuroepithelium Tumors. ... of Chemotherapeutic Drugs and Heterocyclic Compounds at Application on the Cells of Primary Culture of Neuroepithelium Tumors. ...
Breast cancer patient-derived whole-tumor cell culture model for efficient drug profiling and treatment response prediction ... from tumor scraping cells (TSCs) at the time of surgical resection, derived whole-tumor cell (WTC) cultures from each patients ... Panel and cell viability assay data, generated as part of the study "Breast cancer patient-derived whole-tumor cell culture ... Data from: Breast cancer patient-derived whole-tumor cell culture model for efficient drug profiling and treatment response ...
Subsequently, we used a model of neuroinvasion in nude mice to assess the effect of NGF ,i,in vivo,/i, on tumor nerve invasion ... neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. Further, exosomes were ... MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and ... Then RT-qPCR was applied to test miR-21-5p level in tumor derived exosomal (TDE-miR-21-5p). RIP assay was performed to validate ...
Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids Derived from Patients. ... By integrating cellular components of the tumor microenvironment, including T cells, into tumor organoid cultures, immuno- ... Exploring Tumor-Immune Interactions in Co-Culture Models of T Cells and Tumor Organoids De ... To address this limitation, co-culture techniques combining tumor organoids and T cells have been developed, offering diverse ...
... and higher cytokine levels was greater in primary breast and lung carcinoma cells than in ovarian carcinoma cells or tumor cell ... Fifty cell sources, including both immortalized cell lines and primary carcinoma cells, were incubated under normoxic ... Cell culture media from both conditions was collected and analyzed via an ELISA-based assay to determine expression levels of ... We postulated that the expression levels of angiogenesis-related proteins from various primary tumor cultures would be greater ...
"Cell culture, Clear cell sarcoma, Immunotherapy, SCID mouse xenograft, Soft tissue melanoma, Tumour-associated antigens, Tumour ... 深入研究「Newly established MST-1 tumour cell line and tumour-infiltrating lymphocyte culture from a patient with soft tissue ... Newly established MST-1 tumour cell line and tumour-infiltrating lymphocyte culture from a patient with soft tissue melanoma ( ... Newly established MST-1 tumour cell line and tumour-infiltrating lymphocyte culture from a patient with soft tissue melanoma ( ...
... of target cells after 48 h of co-culture. This effect was tumor cell-specific and dependent on CD8(+) T cells evidenced by in ... of target cells after 48 h of co-culture. This effect was tumor cell-specific and dependent on CD8(+) T cells evidenced by in ... of target cells after 48 h of co-culture. This effect was tumor cell-specific and dependent on CD8(+) T cells evidenced by in ... of target cells after 48 h of co-culture. This effect was tumor cell-specific and dependent on CD8(+) T cells evidenced by in ...
Neal Young Weve used subgenomic-replicon-expressing cell lines, liver biopsy examples from… ... Each tumor type reflected the initial phenotype from the parent culture cells. For instance, GS5 cells as well as their tumor ... Hepatitis c virus-caused cancer stem cell-like signatures in cell culture and murine tumor xenografts. June 9, 2017Hepatitis C ... hepatic stem cell-like cells) and CK19+ AFP+ cells (hepatoblast and transit-amplifying cells [61]). These HCV-caused stem cell- ...