DNA End Modification Products | NEB
T4 DNA Ligase Reaction Buffer Close T4 Polynucleotide Kinase Close T4 Polynucleotide Kinase (3 phosphatase minus) Close T4 ... T4 DNA Ligase Reaction Buffer Close T4 Polynucleotide Kinase Close T4 Polynucleotide Kinase (3 phosphatase minus) Close T4 ... We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or ... Polynucleotide Kinase Reaction Buffer A-tailing. Close Deoxynucleotide (dNTP) Solution Set Close Klenow Fragment (3→5 exo-) ...
Charles C. Richardson - Wikipedia
The structural gene for polynucleotide ligase in bacteriophage T4". Proceedings of the National Academy of Sciences of the ... Richardson discovered and researched several enzymes throughout his career: E. coli exonuclease III in 1964, T4 DNA ligase in ... Richardson, Charles C. (January 1, 1981), Boyer, Paul D. (ed.), 16 Bacteriophage T4 Polynucleotide Kinase, The Enzymes, vol. 14 ... 1967, T7 DNA polymerase in 1971, E. coli exonuclease VII in 1974, E. coli DNA polymerase III in 1975, T4 polynucleotide kinase ...
Synthesis of DNA Flashcards
Polynucleotide ligase. Catalyses formation of phosphodiester bonds.. Term. DNA polymerase has what kind of proofreading ...
RNA Ligase (ATP) | Harvard Catalyst Profiles | Harvard Catalyst
Ligases [D08.811.464]. *Polynucleotide Ligases [D08.811.464.754]. *RNA Ligase (ATP) [D08.811.464.754.720] ... "RNA Ligase (ATP)" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... RNA-ligase-dependent biases in miRNA representation in deep-sequenced small RNA cDNA libraries. RNA. 2011 Sep; 17(9):1697-712. ... Archaeal RNA ligase is a homodimeric protein that catalyzes intramolecular ligation of single-stranded RNA and DNA. Nucleic ...
Troubleshooting Guide for End Modification | NEB
We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or ... Supplement the reaction with 1mM ATP, as it is required by T4 Polynucleotide Kinase (NEB #M0201). ... Alternatively, use 1X T4 DNA Ligase Buffer (contains 1 mM ATP) instead of the 1X T4 PNK Buffer ...
GEO Accession viewer
SciELO - Brazil - AFLP and SRAP markers linked to the mj gene for root-knot nematode resistance in cucumber AFLP and SRAP...
The ligation of EcoRI and MseI adapters was performed with T4 DNA ligase (GibcoBRL, Gaithersburg, MD, USA). Preamplification ... 1995). For visualization, EcoRI primers were end-labeled with [γ-33P] ATP using T4 polynucleotide kinase (Invitrogen, Carlsbad ...
High-precision mapping reveals rare N6-deoxyadenosine methylation in the mammalian genome | Cell Discovery
Microsatellite Primers in the Foundation Tree Species Pinus edulis and P. monophylla (Pinaceae)
... were ligated to 3.3 µg of prepared fragments with T4 DNA ligase (Invitrogen, Carlsbad, California, USA) in the presence of the ... Polynucleotide linkers (ScaF: 5′-CAGTGCTCTAGACGTGCTAGT-3′; ScaR: 5′-ACTAGCACGTCTAGAGCACTGAAAA-3′; Eurofins MWG Operon, ...
US Patent for Compounds and methods for modulating activation of NF-κB Patent (Patent # 8,263,326 issued September 11, 2012) -...
Modulating agents include human E3 ubiquitin ligases, antibodies thereto and variants thereof, as well as related proteins. ... Human E3 Ubiquitin Ligase Polypeptides and Polynucleotides. It has been found, within the context of the present invention, ... Within certain embodiments, such polynucleotides may encode a portion of a human E3 ubiquitin ligase, or variant of such a ... present the sequence of a polynucleotide encoding a human E3 ubiquitin ligase (SEQ ID NO: 15). ...
MeSH Browser
Ligases [D08.811.464] * Polynucleotide Ligases [D08.811.464.754] * DNA Ligases [D08.811.464.754.600] * DNA Ligase ATP [D08.811. ... for DNA LIGASES see POLYNUCLEOTIDE SYNTHETASES 1974-77, & see DNA LIGASE 1973-76. History Note. 98(77); for DNA LIGASES use ... DNA Ligase Polydeoxyribonucleotide Ligases Polydeoxyribonucleotide Synthetases T4 DNA Ligase Registry Number. EC 6.5.1.-. ... T4 DNA Ligase Narrower Concept UI. M0017170. Registry Number. 0. Terms. T4 DNA Ligase Preferred Term Term UI T032608. Date02/08 ...
XRCC1
- Early...
US Patent for Asymmetric benzoxanthene dye labelling reagents Patent (Patent # 6,617,445 issued September 9, 2003) - Justia...
... the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and ... Labeled polynucleotides may be synthesized either enzymatically, e.g., using a DNA polymerase or ligase, e.g., Stryer, ... C. Polynucleotide Reagents. Yet another preferred class of reagents of the present invention comprise polynucleotides labeled ... B1 is stable to polynucleotide synthesis conditions yet is able to be removed from the polynucleotide product with a reagent ...
Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene - Wikidata
Enzyme III Progress curve, Michaelis Menten derivation, Determination of Vmax and Km, Catalytic efficiency (Kcat/Km)...
DNA modifying enzymes (ligase, polymerases, alkaline phosphatase, polynucleotide kinase, terminal transferase, Reverse ... DNA modifying enzymes (ligase, polymerases, alkaline phosphatase, polynucleotide kinase, terminal transferase, Reverse ...
Fact file for DNA ligase deficiency IV
Ligase IV, DNA, ATP-dependent. *Polydeoxyribonucleotide synthase. *Polynucleotide ligase. *DNA ligase IV ...
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments | Protocol
10x T4 DNA ligase reaction buffer. 2. 100 μM primer. 2. T4 Polynucleotide kinase (PNK). 1. ... 10x T4 DNA ligase reaction buffer. New England BioLabs. B0202S. T4 Polynucelotide kinase (PNK). New England BioLabs. M0201S. ... In a tube, add the volume calculated in Step 4.4.2.1 of the PCR product, 80 µl of 10× DNA ligase buffer, and distilled H2O up ... Add 4 µl of T4 DNA ligase, and immediately place tubes at 16 °C in a water bath or thermal cycler. Incubate at 16 °C O/N for 14 ...
Browsing Albert Einstein College of Medicine: Doctoral Dissertations by Author "FEINER, LEONARD ALLEN"
THE MECHANISM OF ACTION AND THE CHEMICAL AND PHYSICAL PROPERTIES OF ESCHERICHIA COLI POLYNUCLEOTIDE LIGASE FEINER, LEONARD ...
DNA Ligases | Profiles RNS
DNA Ligases [D08.811.074.500]. *Ligases [D08.811.464]. *Polynucleotide Ligases [D08.811.464.754]. *DNA Ligases [D08.811.464.754 ... "DNA Ligases" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "DNA Ligases" by people in this website by year, and whether " ... Below are the most recent publications written about "DNA Ligases" by people in Profiles. ...
Ayka Medikal
Detection of anticodon nuclease residues involved in tRNA(Lys) cleavage specificity<...
keywords = "DNA restriction-modification, Polynucleotide kinase, RNA ligase, tRNA identity, tRNA splicing endonuclease", ...
OPTiKD
T4 DNA Ligase Reaction Buffer (New England Biolabs, cat. no. B0202S). *T4 Polynucleotide Kinase (New England Biolabs, cat. no. ...
T4 DNA Ligase
T4 DNA Ligase for joining of DNA fragments ... the enzyme-adenylate intermediate in the polynucleotide ligase ... T4 DNA Ligase. 25,000 Units. USD $64.00 T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded ... T4 DNA Ligase. 125,000 Units. USD $247.00 T4 DNA Ligase catalyzes the formation of phosphodiester bonds between double-stranded ... Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4. J. Biol. ...
Gerald Edelman | Academic Influence
Affinity-based enrichment strategies to assay methyl-CpG binding activity and DNA methylation in early Xenopus embryos | BMC...
The concatamerization reaction was performed at RT for 4 h with T4 ligase (Promega) in the presence of 10 mM ATP to replenish ... 42]. The annealed probe was phosphorylated with T4 polynucleotide kinase (New England Biolabs) for 2 h at 37°C, purified by ... the ATP from the ligase buffer. The ligation process was monitored using 1% agarose gel electrophoresis. The reaction was ...
DNA-ligase activity in axolotl early development: evidence for a multilevel regulation of gene expression | Development | The...
Purification and properties of a soluble polynucleotide ligase from chick embryo. . Biochimie ... Exclusion process between DNA-ligase I and DNA-ligase II. Left, diagram of the experiment; centre, DNA-ligase activity after ... Exclusion process between DNA-ligase I and DNA-ligase II. Left, diagram of the experiment; centre, DNA-ligase activity after ... injection of RNA coding for DNA-ligase I and RNA coding for DNA-ligase II, (D) disappearance of preexisting DNA-ligase II in ...
Ólafur H. Friðjónsson - Matís
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1. Nucleic Acids Research ... Characterization of a 5′-polynucleotide kinase/3′-phosphatase from bacteriophage RM378. Biol Chem. 280:5188-94. ... Also filed as PCT as well as five divisional US patent applications on genes for RNA ligase, DNA polymerase, DNA helicase, ... S. Hjörleifsdóttir, G.O. Hreggvidsson, O.H. Fridjonsson, A. Ævarsson & J.K. Kristjansson: RNA Ligase of Bacteriophage RM378, ...
Gene P18887 | Protein XRCC1 - Overview | canSAR Black
Product Center Manufacturers & Suppliers - Product Center Factory - Part 4
Paul O'Higgins - сотрудник | ИСТИНА - Интеллектуальная Система Тематического Исследования НАукометрических данных