The proteins Munc18a, Munc18-interacting protein 1 and Munc18-interacting protein 2 mediate exocytosis and decrease β-amyloid ... Munc18-interacting protein 1 and Munc18-interacting protein 2 in Alzheimers disease cortex and found that this pathway was up- ... Cyclin-dependent kinase 5, Munc18a and Munc18-interacting protein 1/X11α protein up-regulation in Alzheimers disease. ... Munc18a and Munc18-interacting protein 1/X11α protein up-regulation in Alzheimers disease. Neuroscience, 138(2), 511-522. doi: ...

Keywords: ITC; Syntaxin; SNAREs; Membrane Fusion; Neuronal Secretion; Sec1/Munc18; Biochemitry; Protein-Protein Interactions ... Schlagwörter: ITC; Syntaxin; SNAREs; Membrane Fusion; Neuronal Secretion; Sec1/Munc18; Biochemitry; Protein-Protein ... Sec1p/Munc18 (SM) proteins and their role in regulating secretion in Saccharomyces cerevisiae and Caenorhabditis elegans a ... The members of the Sec1p/Munc18 (SM) family of proteins are indispensible regulatory elements that act at the post-docking step ...

Okamoto M, Südhof TC (1998). "Mints, Munc18-interacting proteins in synaptic vesicle exocytosis". J. Biol. Chem. 272 (50): ... The protein encoded by this gene is a member of the X11 protein family. It is a neuronal adaptor protein that interacts with ... Amyloid beta A4 precursor protein-binding family A member 2 is a protein that in humans is encoded by the APBA2 gene. This ... Borg JP, Yang Y, De Taddéo-Borg M, Margolis B, Turner RS (1998). "The X11alpha protein slows cellular amyloid precursor protein ...

A second model for Munc18-1 action is based on the observation that SM (Sec1/Munc18-like) proteins, including Munc18-1, protect ... Competition between native Munc18-1 and overexpressed Munc18-2. Munc18-1, Munc18-2, Munc18-2 NV, or Munc18-1 NV were ... in the presence of Munc18-1. We expressed Munc18 variants (Munc18-1, Munc18-1 NV, and Munc18-2) in munc18-1 −/− cells and ... C , GST-syntaxin1 binding of Munc18-1 and Munc18-2 (WT) and their NV mutants. D , Mean ± SEM binding of Munc18-1 NV and Munc18- ...

Structure-based Functional Analysis reveals novel roles for the Sec1/Munc18 Protein Sly1p in Yeast Org.: Prof. J. Gruenberg ... Protein sorting in the ER in yeast and mammalian cells Org.: Dr Reika Watanabe ... Sorting Nexin 16: a PI3P binding protein involved in MAPK signaling from specialized late endosomal membranes Org.: Dr Reika ... proteins sorting and requirements upon ER exit in yeast Org.: Dr Reika Watanabe ...

Specification and Neurodegenerative Disease-Like Death of Spinal Neurons Lacking the SNARE-Associated Synaptic Protein Munc18-1 ... The Matrix Proteins Hasp and Hig Exhibit Segregated Distribution within Synaptic Clefts and Play Distinct Roles in ... Calcium-Permeable AMPA Receptors Mediate the Induction of the Protein Kinase A-Dependent Component of Long-Term Potentiation in ...

Munc-18-1 interacting protein 1, also named X11α, mLin-10, 120 kDa) and Mint2 (X11β, X11L, 120 kDa), that are 50% homologous. ... The Effective Use of Protein Kinase Inhibitors. CK2 104 98 102 103 103 107 96 87 97 93 98 18 19 104 73 112 104 101 106 PHK 51 ... Product Flyer: Chromolith® WP 300 RP-18 2 mm I.D. Monolithic Silica HPLC Columns - Rapid and Robust Protein Chromatography. ... Mint3 does not interact with Munc-18. Mint3 (X11γ, X11L2, APB3A, 70 kDa) ...

For hydrolysis of proteins in solution, (cross-linked) proteins were precipitated with ethanol. For this, protein samples were ... Ma, C., Li, W., Xu, Y. & Rizo, J. Munc13 mediates the transition from the closed syntaxin-Munc18 complex to the SNARE complex. ... The SeeBlue Plus 2 Pre-Stained Protein Marker (Thermo Scientific) was used as molecular weight protein marker. Protein gels ... Proteins were hydrolysed in a total volume of 100 µL with trypsin (Promega) in 25 mM NH4HCO3, pH 8.5, at an enzyme:protein ...

Low-resolution solution structures of Munc18: Syntaxin protein complexes indicate an open binding mode driven by the Syntaxin N ... Rescue of Munc18-1 and -2 double knockdown reveals the essential functions of interaction between Munc18 and closed syntaxin in ... SNARE-ing the structures of Sec1/Munc18 proteins. Current Opinion in Structural Biology, 29, 44-51. doi: 10.1016/j.sbi.2014.09. ... Structural predictions of the SNX-RGS proteins suggest they belong to a new class of lipid transfer proteins. Frontiers in Cell ...

Three luminal ER proteins whose sequence is known, grp78 ("BiP"), grp94, and protein disulphide isomerase, share the carboxy- ... Regulation of CFTR chloride channels by syntaxin and Munc18 isoforms. Naren, A.P., Nelson, D.J., Xie, W., Jovov, B., Pevsner, J ... Microinjection of mRNA coding for an anti-Golgi antibody inhibits intracellular transport of a viral membrane protein. Burke, B ... The ERD2 gene determines the specificity of the luminal ER protein retention system. Lewis, M.J., Sweet, D.J., Pelham, H.R. ...

Munapsys acts both in the pre- and post-synaptic pathways and in a new protein (Munc-18), a key for the assembly of the SNARE ...

Evidence against roles for phorbol binding protein Munc13-1, ADAM adaptor Eve-1, or vesicle trafficking phosphoproteins Munc18 ... Another signal is a complex of proteins known as the retromer, which helps to determine whether the protein inside an endosome ... Both the EGFR and the DAT proteins have sites to which the molecule ubiquitin-best known as labeling a protein for degradation- ... The Parkinsons disease protein alpha-synuclein disrupts cellular Rab homeostasis. Proc Natl Acad Sci U S A. 2008 Jan 8;105(1): ...

The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their ... SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. SNARE proteins ... The yeast SM protein Vps45p regulates membrane fusion through the trans-Golgi / late endosomal system, and interacts directly ... Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the multiple ...

... proteins are scaffold proteins that contain PSD-95-Discs Large-zona occludens-1 (PDZ), Src homology 3, and guanylat ... Mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. Eur. J. Cell Biol. ... None of the GST-PATJ fusion proteins could bind the Pals1ΔL27N mutant protein. A GST-mLin-7 fusion protein was able to bind ... Several Maguk proteins have been identified as mLin-7 binding partners, including novel proteins that we call proteins ...

keywords = "Docking, F-actin, MUNC18-1, MUNC18-2, MUNC18-3, Neuroendocrine, SM proteins, Secretion", ... Munc18-1-KO MCCs expressing MUNC18-1(V263T) showed normal vesicle docking and exocytosis. These results demonstrate that MUNC18 ... Munc18-1-KO MCCs expressing MUNC18-1(V263T) showed normal vesicle docking and exocytosis. These results demonstrate that MUNC18 ... Munc18-1-KO MCCs expressing MUNC18-1(V263T) showed normal vesicle docking and exocytosis. These results demonstrate that MUNC18 ...

Jiao, J. et al. Munc18-1 catalyzes neuronal SNARE assembly by templating SNARE association. Elife 7, doi:10.7554/eLife.41771 ( ... To apply mechanical force to a target protein in magnetic or optical tweezers4-7, we tether two protein positions, e.g., the N ... Kostrz, D. et al. A modular DNA scaffold to study protein-protein interactions at single-molecule resolution. Nat Nanotechnol ... How chaperone proteins or small molecules like ligands act on the time-evolved, different protein populations can also be ...

use single-molecule imaging to reveal the diffusional signature for the SNARE proteins Munc18-1 and syntaxin-1A during ... Munc18-1WT-mEos2 and Munc18-1Δ317-333-mEos2 were generated from pCMV-Munc18-1WT-emGFP and the Δ317-333 mutant (Martin et al., ... The size of Munc18-1 but not Munc18-1Δ317-333 nanodomains is reduced by secretagogue stimulation. Because Munc18-1 is ... Munc18-1WT-mCherry and Munc18-1Δ317-333-mCherry were made by PCR amplification of Munc18-1 using the forward primer (5′- ...

Background and Objectives Pathogenic STXBP1 variants cause a severe early-onset developmental and epileptic encephalopathy (STXBP1-DEE). We aimed to investigate the natural history of STXBP1-DEE in adults focusing on seizure evolution, the presence of movement disorders, and the level of functional (in)dependence. Methods In this observational study, patients with a minimum age of 18 years carrying a (likely) pathogenic STXBP1 variant were recruited through medical genetics departments and epilepsy centers. Treating clinicians completed clinical questionnaires and performed semistructured video examinations while performing tasks from the (modified) Unified Parkinson Disease Rating Scale when possible. Results Thirty adult patients were included for summary statistics, with video recordings available for 19 patients. The median age at last follow-up was 24 years (range 18-58 years). All patients had epilepsy, with a median onset age of 3.5 months. At last follow-up, 80% of adults had ...

Munc18-1 protein molecules move between membrane molecular depots distinct from vesicle docking sites. ... Interestingly proteins for this pathway are increased in FXS, showing that enhancement of specific proteins may be beneficial ... FXS is thought to be caused by excessive synthesis of specific types of proteins controlled by FMRP, which is mutated in FXS ... Hypersensitivity to mGluR5 and ERK1/2 leads to excessive protein synthesis in the hippocampus of a mouse model of fragile X ...

GAGA factor: a multifunctional pioneering chromatin protein. Chetverina, D., Erokhin, M. & Schedl, P., May 2021, In: Cellular ... The role of CPEB family proteins in the nervous system function in the norm and pathology. Kozlov, E., Shidlovskii, Y. V., ... Affinity, Specificity, and Cooperativity of DNA Binding by Bacterial Gene Regulatory Proteins. Carey, J., Jan 1 2022, In: ...

Munc 18-1 Protein Molecules Move between Membrane Molecular Depots Distinct from Vesicle Docking Sites. Smyth, A. M., Yang, L. ... t-SNARE protein conformations patterned by the lipid microenvironment. Rickman, C., Medine, C. N., Dun, A. R., Moulton, D. J., ... Munc18-1 and syntaxin1: unraveling the interactions between the dynamic duo. Smyth, A. M., Duncan, R. R. & Rickman, C., 2010, ... Munc18-1 prevents the formation of ectopic SNARE complexes in living cells. Medine, C. N., Rickman, C., Chamberlain, L. H. & ...

... a reduction in Munc18-1 protein in the beta-cells of MIPβ1KO mice was observed. Munc18-1 is a syntaxin binding protein that ... A significant reduction in synaptosome associated protein 25kDa (Snap25) and vesicle-associated membrane protein 2 (Vamp2) (p, ... Reduced β1 integrin also leads to a decrease in SNARE proteins Snap25, Vamp2, and Munc18-1, indicating β1 integrin is important ... Examination of the exocytotic proteins involved in insulin secretion showed reduced Vamp2 and Snap25 mRNA and protein levels in ...

Sec1/munc18-like (SM) proteins superfamily. ATVPS33, VACUOLAR PROTEIN SORTING. 33. 0.64. 0.31. -0.3. ... phloem protein 2-A1. phloem protein 2-A1, phloem. protein 2-A1, phloem protein 2-A1. 0.79. 0.32. -0.31. ... Ribosomal protein L1p/L10e family. -0.73. 0.32. -0.31. 100. AT5G61770. PETER PAN-like protein. PETER PAN-like protein. -0.73. ... NAP1-related protein 1. NAP1-related protein 1. -0.74. 0.29. -0.29. 78. AT1G79470. Aldolase-type TIM barrel family protein. - ...

SM (Sec1/Munc-18) family protein. Gene synonym. Keyword. -. -. Strain/accession/mutant. Description. ...

... and nSec/Munc18 (SM) proteins that organize the SNARE proteins into membrane fusion-ready SNARE complexes (1). Insulin ... Impaired gene and protein expression of exocytotic soluble N-ethylmaleimide attachment protein receptor complex proteins in ... The detergent pellet extract (600 μg protein) was then precleared (35 μL protein A agarose [50% slurry], 1.5 hours) and ... along with cognate Munc18 and SNARE proteins. These precise mechanistic insights were not elucidated in the Kunii et al. study ...

Different Munc18 proteins mediate baseline and stimulated airway mucin secretion. JCI Insight. 2019 03 21; 4(6). PMID: 30721150 ... Munc18-2, but not Munc18-1 or Munc18-3, regulates platelet exocytosis, hemostasis, and thrombosis. J Biol Chem. 2019 03 29; 294 ... Munc18-2, but not Munc18-1 or Munc18-3, controls compound and single-vesicle-regulated exocytosis in mast cells. J Biol Chem. ... Munc13 proteins control regulated exocytosis in mast cells. J Biol Chem. 2018 01 05; 293(1):345-358. PMID: 29141910; PMCID: ...

... prevents colocalization of the proteins and also the FRET signal. Thus, a protein-protein interaction between Munc 18 and ... CFP-Munc 18 is present in the cytoplasm as expected for a protein lacking membrane targeting domains. However, Munc 18 is ... We have fused syntaxin and Munc 18 to the yellow- (YFP) or cyan-fluorescence-protein (CFP) and expressed the constructs in CHO ... STX8 silencing reduced the binding of quality control protein, Hsp 27, a protein that targets ΔF508-CFTR for sumoylation and ...

Surfactant protein C mutation links postnatal type 2 cell dysfunction to adult disease. JCI Insight. 2021 07 22; 6(14). ... Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1. J Neurosci. 2021 09 01; 41(35): ... Proteomic analysis identifies ZMYM2 as endogenous binding partner of TBX18 protein in 293 and A549 cells. Biochem J. 2022 01 14 ...

Selective activation of cognate SNAREpins by Sec1/Munc18 proteins Journal Article * Structural Basis of Selective ... Increasing and Decreasing the Ultrastability of Bacterial Chemotaxis Core Signaling Complexes by Modifying Protein-Protein ... Programmed Cell Death Protein 5 Interacts with the Cytosolic Chaperonin Containing Tailless Complex Polypeptide 1 (CCT) to ... Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE-Sec1/Munc18 membrane fusion complex ...

Overview: STXBP3 (Syntaxin Binding Protein 3) is a Protein Coding gene. Diseases associated with STXBP3 include Hemophagocytic ... Synonyms: STXBP3, MUNC18-3, MUNC18C, PSP, UNC-18C. Reactivity: Human, Mouse, Rat ...