Three benchtop high-throughput sequencing instruments are now available. The 454 GS Junior (Roche), MiSeq (Illumina) and Ion ... The MiSeq had the highest throughput per run (1.6 Gb/run, 60 Mb/h) and lowest error rates. The 454 GS Junior generated the ... the Ion Torrent PGM had the highest throughput (80-100 Mb/h). Unlike the MiSeq, the Ion Torrent PGM and 454 GS Junior both ... We compared the performance of these instruments by sequencing an isolate of Escherichia coli O104:H4, which caused an outbreak ...

High-Throughput Nucleotide Sequencing * Humans * Neoplasm Proteins / blood * Neoplasm Proteins / genetics* * Prognosis ... Both tumour tissue types, together with a blood sample, will then be subjected to next generation sequencing for a panel of ...

High-Throughput Nucleotide Sequencing* * Mice * RNA* / biosynthesis * RNA* / genetics * Staining and Labeling / methods ... SLAM-ITseq: sequencing cell type-specific transcriptomes without cell sorting Development. 2018 Jul 11;145(13):dev164640. doi: ... Here, we report a novel in vivo metabolic RNA sequencing method, SLAM-ITseq, which metabolically labels RNA with 4-thiouracil ...

Programmable concatenation of cDNA molecules increases the throughput of PacBio sequencing about 15-fold. ... but their throughput is limited. We introduce multiplexed arrays isoform sequencing (MAS-ISO-seq), a technique for programmably ... When applied to single-cell RNA sequencing of tumor-infiltrating T cells, MAS-ISO-seq demonstrated a 12- to 32-fold increase in ... concatenating complementary DNAs (cDNAs) into molecules optimal for long-read sequencing, increasing the throughput >15-fold ...

Minimum Information about a high-throughput nucleotide SEQuencing Experiment. omics Genomics Proteomics Metabolomics Venomics ... producing expressed sequence tags (ESTs). The Sanger method of sequencing was predominant until the advent of high-throughput ... and sequenced using low-throughput, but long read-length methods such as Sanger sequencing. The sequences are then divided back ... Although higher-throughput methods are now used, EST libraries commonly provided sequence information for early microarray ...

High-Throughput Nucleotide Sequencing. *Transcription Factors. *ALK. *Base Sequence. *Genome-Wide Association Study ... Nucleotide sequence of the MYH9-ALK chimeric cDNA revealed that the ALK breakpoint was different from all those previously ... Whole-exome sequencing of his tumor revealed a simple genome landscape with no evidence of mutations, copy-number changes, or ... It is localized in the same exonic sequence as MSN-ALK, but 6 bp downstream, resulting in an in-frame fusion of the two partner ...

High-Throughput Nucleotide Sequencing. *Immunoenzyme Techniques. *Calcium-Binding Proteins. *Phenotype. *Signal Transduction ... MiRNA-373 reported the highest diagnostic efficacy as compared to miRNA-21 and miRNA-222 with high area under the curve (AUC ... 14 responded to NACT and the model generated high-risk RS in 14 (100%); conversely, of 16 cases generating high-risk RS, only 2 ... A high proportion of Her-2/neu gene amplified cases indicates aggressive disease in that area and need for FISH testing on ...

... using high-throughput sequencing (HTS) and comparing sequences from a patient with MCC with sequences from healthy controls, we ... Phylogenetic reconstructions were based on separate analyses of nucleotide sequences from viral protein 1 (VP1) and large T ... MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004;32:1792-7. DOIPubMedGoogle ... High-Throughput Sequencing. HTS was performed by using the Illumina HiSeq 2000 apparatus (Illumina Inc., San Diego, CA, USA) at ...

High-Throughput Nucleotide Sequencing. *Direct-To-Consumer Screening and Testing. *Insurance Coverage ... perspectives on insurance coverage for genome sequencing versus exome sequencing: A study by the Clinical Sequencing Evidence- ... The price of whole-genome sequencing may be decreasing, but who will be sequenced? Per Med. 2017 May; 14(3):203-211. ... Genomic sequencing: assessing the health care system, policy, and big-data implications. Health Aff (Millwood). 2014 Jul; 33(7 ...

Next generation sequencing of ancient DNA 2012. Dr Russell McLaughlin From single nucleotide polymorphisms to high-throughput ... Next-generation sequencing of ancient human DNA, population genetics. 2015. Dr Ian Richardson The Genetics of Mycobacterium ... sequencing in the complex genetics of amyotrophic lateral sclerosis 2011. Dr Yonas Kassahun Hirutu Genomewide admixture mapping ...

... high-throughput sequencing technology. This approach identified genetic markers (SNPs, or single nucleotide polymorphism) ... high-throughput sequencing technology. This method produced 681,257 SNP markers distributed across the entire genome, with the ... We genotyped 2,815 maize inbred accessions, most of them from the NCRPIS, using Genotyping-by-Sequencing (GBS), a low cost, ... 681,257 SNP markers were distributed across the entire maize genome, and most alleles were detected as rare with high ...

... sequencing technologies have progressed toward higher throughput and lower cost per nucleotide (Metzker 2005). The current ... Because these approaches will rely on novel data types such as DNA sequences and high-throughput phenotyping images, Breeding 4 ... from DNA sequences) or analyzing high-throughput phenotyping data for predicting component traits (from images). Realistic ... DNA sequences may be augmented with functional annotations at the level of single nucleotides such that input sequences include ...

High-Throughput Nucleotide Sequencing/methods * Humans * Infant * Infant, Newborn * Leukemia, Megakaryoblastic, Acute/ ... No exome-sequenced TMD/AMKL sample had other recurrently mutated genes. However, 2 of 5 TMD cases, and all AMKL cases, showed ... Exome sequencing identifies putative drivers of progression of transient myeloproliferative disorder to AMKL in infants with ... Exome sequencing identifies putative drivers of progression of transient myeloproliferative disorder to AMKL in infants with ...

... this bar plot describes the proportion of each nucleotide called for every cycle of sequencing. ... Rqc is an optimized tool designed for quality control and assessment of high-throughput sequencing data. It performs parallel ... Rqc - Quality Control Tool for High-Throughput Sequencing Data. Welliton Souza1* and Benilton Carvalho1 ... This version of Rqc produces high-quality images for the following statistics:. *Average Quality: this plot describes the ...

With the development of high throughput technologies such as tandem mass spectrometry (MS/MS) and next generation sequencing, ... more and more nonsynonymous single-nucleotide variations (nsSNVs) that cause variation of amino acids have been identified, ... With the development of high throughput technologies such as tandem mass spectrometry (MS/MS) and next generation sequencing, ... With the development of high-throughput sequencing technology, gene mutation detection has become another important resource to ...

The traditional Sanger method is still popular in clinical applications, but high-throughput next generation sequencing (NGS) ... DNA sequencing refers to a range of techniques that analyze sections of the genome to single-nucleotide resolution. ... The key reason for choosing NGS for clinical diagnostics is its combination of high throughput, speed, and resolution. Like ... myADLM.org // Clinical Laboratory News // CLN Industry Insights // Clinical NGS Initiatives: Making an Impact with Sequencing ...

TreatmentCamptothecinAmyotrophic lateral sclerosisNucleotide sequence 79. A Versatile High Throughput Microfabricated Platform ... Black Lives Matter in Higher Education: Empowering Student-Scholar Voices Tobar, Cynthia 2023 Theses Education, HigherAfrican ... Cells--AnalysisOncologyNucleotide sequenceAdenocarcinomaPancreas--CancerMammals--Cytology 55. A pathogenic function of ... Education--PhilosophyHigh school teachersCoaches (Athletics)CooksTeachers--Attitudes 83. Beyond Worst-Case Analysis for ...

18-200 nucleotides) and long ncRNAs (,200 nucleotides). miRNAs are the most commonly studied type of ncRNA (19,20). circRNAs, ... Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC. *Authors: * ... Liu X, Zeng L, Wang W, Li Z, Zhou S, Wang F, Wang Y, Du J and Ma X: Integrated analysis of high‑throughput sequencing reveals ... Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F. ... Ma, X. (2023). Integrated analysis of high‑throughput sequencing ...

Albers and colleagues applied high-throughput sequencing in 5 unrelated patients with TAR syndrome and the chromosome 1q21.1 ... Absence of mutations in the HoxA10, HoxA11 and HoxD11 nucleotide coding sequences in thrombocytopenia with absent radius ... However, Fleischman and colleagues did not detect mutations in the coding sequence of HOX genes known to affect radial ... 9] They discovered one low frequency single nucleotide polymorphism (SNP) in the noncoding 5 untranslated (UTR) region of the ...

High-Throughput Nucleotide Sequencing; Humans; INDEL Mutation; North America; Phylogeny; Polymorphism, Genetic; Recurrence; ... Each of the HSV-2 genome sequences was initially obtained using next-generation sequencing and completed with Sanger sequencing ... The sequence of each subjects well-documented primary isolate was compared with the sequence of the isolate from their fifth ... We used next-generation sequencing techniques to determine whole-genome sequences of four viral samples from two subjects in ...

High-Throughput Nucleotide Sequencing 20% * Intestinal Mucosa 22% * Intestines 19% * Leukocytes 49% ...

A new high-density genetic linkage map was constructed by using specific locus amplified fragment sequencing (SLAF-seq) ... 2014). Construction and analysis of high-density linkage map using high-throughput sequencing data. PLoS One 9:e98855. doi: ... The map contains 2,808 single-nucleotide polymorphism markers distributed on 20 linkage groups with a total length of 1,308.20 ... High-Throughput SLAF Sequencing and Genotyping. A total of 472.94 GB of raw data containing 1,576.48 M reads were obtained for ...

We directly sequenced cell-free DNA with high-throughput shotgun sequencing technology from plasma of pregnant women, obtaining ... averaging 169 nucleotides in length. Quake proposed a "shotgun sequencing" strategy where he would use the very latest high- ... throughput gene sequencing technology and equally powerful computers to identify millions of unique sequence "tags" in the ... Direct sequencing also allowed us to study the characteristics of cell-free plasma DNA, and we found evidence that this DNA is ...

we used target enrichment and high-throughput methods to sequence ∼10,000 genome-wide bovine single-nucleotide polymorphisms ( ...

Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. Embnet. J. 2011, 17, 10-12. [Google ... Sequencing reads from this study were deposited in the European Nucleotide Archive under the project accession number ... The microbial community composition was analyzed by high-throughput sequencing of the extracellular and intracellular DNA pools ... A sequence cut off number of 40 was set based on artifact sequences occurring in the positive control (E. coli DNA) sample. ...

High-Throughput Nucleotide Sequencing. *Gene Library. *Developmental Biology. *0601 Biochemistry and Cell Biology ... Using this method we have been able to sequence an individuals OR sub-genome with high coverage, enabling us to identify ... in next-generation sequencing have given us the opportunity to determine the sequence of the entire OR gene set with high ... "Next-generation sequencing of the human olfactory receptors." Methods Mol Biol, vol. 1003, 2013, pp. 133-47. Pubmed, doi: ...

Abstract The development of next generation sequencing (NGS) and high throughput genotyping are important techniques for the ... High-resolution melting (HRM) is an emerging technology used for detecting single-nucleotide polymorphisms (SNPs) in various ... Identification of Polymorphic Markers by High-Resolution Melting (HRM) Assay for High-Throughput SNP Genotyping in Maize ... The results showed that the photosynthetic rate of the mutant was significantly higher than that of the control and the rate of ...

... detected is limited by the relatively high rate with which current DNA sequencing technologies incorrectly identify nucleotides ... An entirely different application of high-throughput sequencing is to selectively capture and sequence stretches of DNA or RNA ... The ability to perform sequencing with such high throughput has revolutionized the study of biological systems, but the types ... One class of applications of high-throughput sequencing involves identifying genetic variation, such as finding rare mutations ...

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Res. ... High throughput sequencing-based DNA barcoding. DNA barcoding is traditionally based on Sanger sequencing, requiring separate ... Kennedy, S.R., Prost, S., Overcast, I. et al. High-throughput sequencing for community analysis: the promise of DNA barcoding ... The emergence of high-throughput sequencing technologies (HTS) has been a significant step forward in recent years, greatly ...