Fluorescence polarization immunoassay - Wikipedia
The solution is evaluated again by the fluorescence polarization analyzer. The fluorescence polarization value for the tracer ... "Fluorescence Polarization Immunoassay II. Analyzer for Rapid, Precise Measurement of Fluorescence Polarization with Use of ... and is measured by a fluorescence polarization analyzer. Fluorescence polarization was first observed by F. Weigert in 1920. He ... which results in altered fluorescence polarization. Fluorescence polarization immunoassays employ a fluorophore bound antigen ...
Aptamer-based fluorescence polarization assay for separation-free exosome quantification - Nanoscale (RSC Publishing)
Aptamer-based fluorescence polarization assay for separation-free exosome quantification Z. Zhang, C. Tang, L. Zhao, L. Xu, W. ... Here we have developed an aptamer-based fluorescence polarization assay for exosome quantification, which is a separation-free ... inherent huge mass/volume acts as mass-based fluorescence polarization amplifier. Our assay allows quantitative analysis of ...
Erowid.org: Erowid Reference 512 : Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit...
Schwarzhoff R Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine ... "Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine analogues". J Anal ... "Fluorescence polarization immunoassay detection of amphetamine, methamphetamine, and illicit amphetamine analogues" J Anal ...
RePub, Erasmus University Repository:
Using fluorescence polarization immunoassay for determination of erythrocyte...
Using fluorescence polarization immunoassay for determination of erythrocyte methotrexate polyglutamates, a quick and easy test ... den Boer, E, Koch, B.C.P, Huisman, R, & de Jonge, R. (2014). Using fluorescence polarization immunoassay for determination of ... METHODS:: Samples were analyzed by an adapted fluorescence polarization immune assay (FPIA) method on the FLx analyzer (Abbott ...
2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson's...
2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinsons ... This imaging method assesses homo-FRET between labels by measuring fluorescence polarization in excitation and emission ... This imaging method assesses homo-FRET between labels by measuring fluorescence polarization in excitation and emission ... Nanoscale organization of luminescent materials and their polarization properties investigated by two-dimensional polarization ...
Fluorescence Anisotropy Reloaded-Emerging Polarization Microscopy Methods for Assessing Chromophores' Organization and...
Fluorescence Anisotropy Reloaded-Emerging Polarization Microscopy Methods for Assessing Chromophores Organization and ... Fluorescence Anisotropy Reloaded-Emerging Polarization Microscopy Methods for Assessing Chromophores Organization and ... Fluorescence Anisotropy Reloaded-Emerging Polarization Microscopy Methods for Assessing Chromophores Organization and ...
Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric...
Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric ... Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric ... "Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric ... Time-, wavelength- and polarization-resolved measurements of OH (A2Sigma+) picosecond laser-induced fluorescence in atmospheric ...
The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction | Nature...
b Western blot, c fluorescence polarization and d native PAGE analyses confirm epichaperome network formation upon human tau ... Fluorescence polarization assay. For epichaperome inhibition measurements, assays were carried out in black 96-well microplates ... 5e, f). Evidence was provided by fluorescence polarization where increased binding of the PU-FITC probe indicates greater ... then fluorescence of resorufin (reduced form of resazurin) was measured using a Molecular Devices SpectraMax i3 fluorescence ...
The epichaperome is a mediator of toxic hippocampal stress and leads to protein connectivity-based dysfunction | Nature...
b Western blot, c fluorescence polarization and d native PAGE analyses confirm epichaperome network formation upon human tau ... Fluorescence polarization assay. For epichaperome inhibition measurements, assays were carried out in black 96-well microplates ... 5e, f). Evidence was provided by fluorescence polarization where increased binding of the PU-FITC probe indicates greater ... then fluorescence of resorufin (reduced form of resazurin) was measured using a Molecular Devices SpectraMax i3 fluorescence ...
Synthesis of a high-affinity Fluorescent PPARγ ligand for high-throughput fluorescence polarization assays<...
Synthesis of a high-affinity Fluorescent PPARγ ligand for high-throughput fluorescence polarization assays. In: Bioorganic and ... Scott Martin for assistance with fluorescence polarization measurements. We thank the NIH (R01-CA83831 to B.R.P. and R01- ... Scott Martin for assistance with fluorescence polarization measurements. We thank the NIH (R01-CA83831 to B.R.P. and R01- ... Synthesis of a high-affinity Fluorescent PPARγ ligand for high-throughput fluorescence polarization assays. Bioorganic and ...
Cancer diagnosis by fluorescence polarization - Fingerprint - Bar-Ilan University
E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP | bioRxiv
Reactions were monitored by measuring the fluorescence polarization over time using the 485-520-520 fluorescence polarization ... Change in polarization over time is used as the measure of apparent activity. (b) Fluorescence polarization timecourse curves ... as quantified from fluorescence polarization timecourse curves. Change in polarization over time is used as the measure of ... Fluorescence polarization-based ubiquitination assays. The E3 ligase activity of RNF213 variants was measured using a ...
High Throughput Screening: The Discovery of Bioactive Substances - John P. Devlin - Google Books
... defined described detection determine diversity drug discovery effective enzyme evaluated example extracts Figure fluorescence ...
Measurement of Fluorescein Binding in Human Plasma Using Fluorescence Polarization | JAMA Ophthalmology | JAMA Network
Measurement of Fluorescein Binding in Human Plasma Using Fluorescence Polarization. Richard F. Brubaker, MD; John T. Penniston ... Technical difficulties were encountered with the fluorescence polarization method above fluorescein concentrations of 10-5 g/mL ... and fluorescence polarization. All methods indicated that fluorescein is approximately 85% bound throughout a wide range of ... Measurement of Fluorescein Binding in Human Plasma Using Fluorescence Polarization. Arch Ophthalmol. 1982;100(4):625-630. doi: ...
Analysis of Sulfamethazine by fluorescence polarization immunoassay - статья | ИСТИНА - Интеллектуальная Система Тематического...
Analysis of Sulfamethazine by fluorescence polarization immunoassayстатья Информация о цитировании статьи получена из Web of ... Аннотация: A fluorescence polarization immunoassay (FPIA) based on a polyclonal antibody for the determination of ...
Quantitative proteomics of the thyroid hormone receptor-coregulator interactions
Fluorescence Polarization * Humans * Molecular Sequence Data * Peptide Library * Peptides / chemistry * Peptides / genetics ...
Second-trimester serum fluorescence polarization in patients who subsequently develop pregnancy-induced hypertension<...
Barkai, G, Ries, L, Lusky, A, Korach, J, Mashiach, S & Schiff, E 1996, Second-trimester serum fluorescence polarization in ... Second-trimester serum fluorescence polarization in patients who subsequently develop pregnancy-induced hypertension. In: ... Second-trimester serum fluorescence polarization in patients who subsequently develop pregnancy-induced hypertension. / Barkai ... Second-trimester serum fluorescence polarization in patients who subsequently develop pregnancy-induced hypertension. American ...
RNA-binding proteins distinguish between similar sequence motifs to promote targeted deadenylation by Ccr4-Not | eLife
We used fluorescence polarization experiments to examine RNA binding and found that S. pombe Puf3PUM binds to both the ScPuf3 ... Fluorescence polarization assays. Request a detailed protocol A two-fold protein dilution series was prepared in 20 mM HEPES pH ... A) Fluorescence polarization experiments assaying the binding of Puf3 RNA-binding domain to RNA containing a ScPuf3 motif (blue ... B) Fluorescence polarization experiments assaying the binding of full-length Puf3 (top) or Zfs1 (bottom) to 5ʹ−6FAM labelled ...
A high throughput fluorescence polarization assay for inhibitors of the GoLoco motif/G-alpha interaction - Fingerprint -...
Measuring the Kinetics of the Binding of Xenoestrogens and Estrogen Receptor Alpha by Fluorescence Polarization - Fingerprint ...
Journal of Research Volume 79A | NIST
HTS Multimode Microplate Reader, BioTek Synergy Neo2 | Agilent
Fluorescence polarization Fluorescence polarization (FP) measurements are made using an optical system that includes polarizing ... Time-resolved fluorescence Time-resolved fluorescence (TRF) measurement is very similar to fluorescence intensity (FI) ... Monochromator: variable bandwidth, absorbance, fluorescence, luminescence Filters: fluorescence polarization, time-resolved ... Time-resolved fluorescence energy transfer (TR-FRET) works on the principles that when suitable pairs of fluorophores are in ...
Fluorescence polarisation activity-based protein profiling for the identification of deoxynojirimycin-type inhibitors selective...
NHANES 2003-2004: Methylmalonic acid & Homocysteine Data Documentation, Codebook, and Frequencies
The method is a fluorescence polarization immunoassay (FPIA) from Abbott Diagnostics. Total homocysteine (tHcy) in plasma is ...
Publications | Computational Biology and Drug Design Group
Upconversion photoluminescence appears to shine polarized and brighter
Polarization has been extensively employed in the fluorescence polarization imaging technique. The inherent upconversion ... Polarization is another important characteristic of fluorescence. It provides orientation and structural information in an ... By controlling the rotation of the polarization analyzer, the team demonstrated linearly- and cross-polarized emission at dual ... It provides an excellent alternative to fluorescence enhancement. Collective high-Q resonances emerge due to coupling these Mie ...
PAR-08-034: Solicitation of Assays for High Throughput Screening (HTS) in the Molecular Libraries Probe Production Centers...
e.g. fluorescence polarization or radiometric counting). The proposed assay should demonstrate highly robust and reproducible ... The MLPCN will be capable of implementing assays using a variety of detection readouts such as absorbance, fluorescence, ... fluorescence, FLIPR, FRET, SPA, absorbance, ELISA, microscopy-based imaging, and small molecule microarray); 2). To develop a ... luminescence, fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET), biophysical ...
BIOENGINEERING
... polarization, and fluorescence; confocal and super-resolution microscopy; and image processing and machine learning strategies ... polarization, and fluorescence; confocal and super-resolution microscopy; and image processing and machine learning strategies ... Evaluates designs using sequencing, fluorescence assays, enzyme activity assays, and single cell studies using time-lapse ...
FRET-based Measurements of Protein Complexes to Determine Stoichiometry | BMG LABTECH
Competition assay using Fluorescence Polarization to determine the Residence Times for Calcitonin and AMYR agonist, AM833. ... Ubiquitination monitoring in real-time: the fluorescence polarization-based method UbiReal. Track ubiquitination processes in ... to polyubiquitination using TAMRA-labelled ubiquitin and the CLARIOstars excellent fluorescence polarization detection. ...