Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells | Citedby Results | Journal of Cell...
A number of different assay methods have been used to study repair of strand breaks in DNA after exposure of cells to ionizing ... Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells. J Cell Sci (1981) 48 (1): 383-391. ... Inhibition of DNA synthesis and alteration to DNA structure by the phenacetin analog p-aminophenol ... Differences in the DNA Supercoiling Response of Irradiated Cell Lines from Ataxia-telangiectasia Versus Unaffected Individuals ...
Reconstruction of complexes of histone and superhelical nuclear DNA. - Oxford Neuroscience
The superhelical status of the DNA in the reconstructed complexes is indistinguishable from that found in control nucleoids ... These nucleoids contain all the nuclear RNA and DNA but few of the proteins characteristic of chromatin. Their DNA is ... Experiments with micrococcal nuclease confirm that the DNA in the reconstructed complexes is organized into nucleosome-like ... histones without breaking the DNA. We have probed the integrity and structure of the reconstructed complexes using a non- ...
Recombination promoted by superhelical DNA and the recA gene of Escherichia coli. | Microbiology and Immunology
Coliphages, DNA, Bacterial, DNA, Recombinant, DNA, Single-Stranded, DNA, Viral, Escherichia coli, Genes, Nucleic Acid ... When a mixture of superhelical DNA (RFI) of phage phiX174 am3 and fragments of single-stranded DNA from wild-type phiX174 was ... Recombination promoted by superhelical DNA and the recA gene of Escherichia coli.. ... Recombination promoted by superhelical DNA and the recA gene of Escherichia coli.. ...
DNA supercoil - Wikipedia
Å of DNA length. Wr, called "writhe," is the number of superhelical twists. Since biological circular DNA is usually underwound ... DNA supercoiling is important for DNA packaging within all cells. Because the length of DNA can be thousands of times that of a ... Negative supercoiling is also thought to favour the transition between B-DNA and Z-DNA, and moderate the interactions of DNA ... Supercoiling is also required for DNA/RNA synthesis. Because DNA must be unwound for DNA/RNA polymerase action, supercoils will ...
Targeted gene correction by small single-stranded oligonucleotides in mammalian cells
DNA Repair / genetics* * DNA, Superhelical / genetics * Gene Targeting / methods* * Genetic Therapy / methods ... showed a similar gene correction frequency to chimeric RNA-DNA oligonucleotide, measured using the same system. The in vitro ... at a similar frequency as the chimeric RNA-DNA oligonucleotide. ...
Research | Max Planck Institute of Biochemistry
... nucleosomes function as the most basic unit of chromosome organization directly binding and assembling on DNA to modulate DNA ... Likewise, superhelical tension forms on the daughter strands as a consequence of polymerase synthesis. Polymerases must travel ... They consist of a dimer of dimers with two copies each of the histones H3, H4, H2A and H2B, that tightly bind and wrap DNA, ... Resolution of superhelical tension is critical to ensure replication fork progression and avoid chromosome damage. To date, ...
Cryo-EM structure of SMG1-SMG8-SMG9 complex | Cell Research
30 DNA-PKcs in DNA-PK holoenzyme (PDB ID: 5Y3R),32 and ATR in ATR-ATRIP complex (PDB ID: 5YZ0).31 The structures are shown in ... 2). (1) In other PIKK family members, the N-HEAT domains more or less adopt a twisted super-helical conformation. In contrast, ... b-e The structures of SMG1 (b), mTOR (c), ATR (d) and DNA-PKcs (e) are shown as in a. f Close-up view of the superimposed KDs ... g-j Close-up views of the four-way junction of SMG1 (g), and equivalent regions in mTOR (h), ATR (i) and DNA-PKcs (j). SMG1 and ...
Research publications | IRB Barcelona
CATH Domain 1bgwA05
POLE2 DNA polymerase epsilon 2, accessory subunit [Homo sapiens (human)] - Gene - NCBI
... the N-terminal domain of human DNA polymerase epsilon subunit B revealed a domain that consists of a left-handed superhelical ... DNA polymerase epsilon subunit 2. Names. DNA polymerase II subunit 2. DNA polymerase epsilon subunit B. polymerase (DNA ... DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B. pfam12213. Location:4 → 73. Dpoe2NT; DNA polymerases epsilon N terminal. ... DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B. pfam12213. Location:4 → 73. Dpoe2NT; DNA polymerases epsilon N terminal. ...
Genetics<...
We will discuss the mechanism of DNA supercoiling and the role of superhelical stress in DNA structure and function. Mechanism ... Introduction DNA supercoiling is the twisting or coiling of the double helix structure of DNA, resulting in a strain on the ... Principle of DNA MicroarrayThe principle of DNA microarray technology is based on the hybridization of complementary sequences ... Introduction DNA microarrays are powerful tools for analysing gene expression patterns, detecting DNA mutations, and ...
PDF) Mapping of scaffold/matrix attachment regions in human genome: a data mining exercise
... are DNA elements that serve to compartmentalize the chromatin into structural and functional domains.... , Find, read and cite ... protein-DNA complexes. We find that although protein binding may bend the DNA, bending alone is not sufficient to kink the DNA ... also susceptible to superhelical stress-induced duplex desta-. bilization (56). OriC is one such element that contains AT ... and has been used as a method of choice for studying DNA conformation, secondary DNA structure transition and DNA damage. In ...
I. The structure and properties of closed circular duplex DNA. II. Chemically interacting systems at equilibrium in a buoyant...
The results reveal that SV 40 DNA I contains about -13 superhelical turns in concentrated salt solutions. ... The number of superhelical turns, τ, present in I is calculated at each dye concentration using Fuller and Warings (1964) ... The theory is applied to the specific cases of the binding of ethidium bromide to SV 40 DNA and of the binding of mercury and ... of τ from a consideration of the effect of the superhelical turns upon the binding isotherm of ethidium bromide to SV 40 DNA I ...
Katerina Gurova | Roswell Park Comprehensive Cancer Center - Buffalo, NY
Thus FACT has been a sensor and mediator of a novel type of cell stress-DNA superhelical or torsional stress, which is the ... We also believe that FACT may be involved in the protection of our genome from DNA damage through the control of DNA topology. ... Our current focus area is chromatin remodeling factors, their role in normal development and disease, DNA damage detection and ... Specifically, she identified a new class of prospective anticancer agents named curaxins which interfere with DNA binding to ...
Polymerase II And additionally Need Activator, Intermediary, and you can Chromatin-modifying Necessary protein - مطاعم الصفوانى
Transcription Elongation Provides Superhelical Tension for the DNA. Immediately following it offers initiated transcription, ... not, because the chatted about into the Chapter cuatro, DNA when you look at the eucaryotic tissue was packaged to the ... Indeed, these additional necessary protein assemblies get get in touch with one another away from the DNA and start to become ... Just like the demonstrated into the Chapter 7, these necessary protein join to certain brief sequences into the DNA. In the ...
SMART: Schnipsel domain TOPEUc
DNA topoisomerases regulate the number of topological links between two DNA strands (i.e. change the number of superhelical ... Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex ... CRYSTAL STRUCTURE OF HUMAN TOPOISOMERASE I DNA COMPLEX. 1k4s. HUMAN DNA TOPOISOMERASE I IN COVALENT COMPLEX WITH A 22 BASE PAIR ... HUMAN DNA TOPOISOMERASE I (70 KDA) IN COMPLEX WITH THE POISON TOPOTECAN AND COVALENT COMPLEX WITH A 22 BASE PAIR DNA DUPLEX. ...
The WOW Files: Scientists Confirm A Second Layer Of Information Has Been Found Hiding Inside DNA
... into a lefthanded superhelical path that mimics the DNA conformation in the nucleosome crystal structure [4]. (B) Fraction of ... So your DNA could stretch to the sun and back 61 times. That is one persons DNA." For all FEELguide stories related to DNA be ... SEE ALSO: Digital DNA: A Mindblowing Conversation About The Future Of Synthetic DNA In The Digital Age. SEE ALSO: Researchers ... SEE ALSO: Scientists Stunned By Meditations Ability To Regrow DNAs Age-Defying Telomeres In Cancer Patients. SEE ALSO: DNA ...
Formation of DNA triplexes accounts for arrests of DNA synthesis at d(TC)n and d(GA)n tracts - Wikidata
Intramolecular DNA triplexes, bent DNA and DNA unwinding elements in the initiation region of an amplified dihydrofolate ... Formation of DNA triplexes accounts for arrests of DNA synthesis at d(TC)n and d(GA)n tracts. scientific article published on ... Formation of DNA triplexes accounts for arrests of DNA synthesis at d(TC)n and d(GA)n tracts (English) ... The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions ...
Size exclusion chromatography of plasmid DNA isoforms - McMaster Experts
DNA, Superhelical (MeSH) * Electrophoresis, Agar Gel (MeSH) * Models, Theoretical (MeSH) * Nucleic Acid Conformation (MeSH) ... The partition coefficient also increased with increasing ionic strength due to the compaction of the DNA associated with the ...
Chromatin assembly factor 1. Medical search
DNA, Superhelical. Circular duplex DNA isolated from viruses, bacteria and mitochondria in supercoiled or supertwisted form. ... It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into ... Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a ... GeneticDNA, SuperhelicalMicrococcal NucleaseRibosome Subunits, Large, EukaryoticDNACentromereTranscription FactorsProtein ...
Transposon Tn5 target specificity: preference for insertion at G/C pairs.
The procaryotic transposon Tn5 inserts into many different sites within a single gene, but some sites (hotspots) are targeted repeatedly. Hotspots are not closely related in sequence, but most have G/C pairs at the ends of the nine base pairs duplicated by Tn5 insertion. In pBR322, the major hotspot coincides with the -10 region of the tet promoter. We mutated the G/C pairs at this hotspot and assayed for insertion into hotspot I, resistance to tetracycline, and plasmid supercoiling. We found that changing the G/C pairs to A/T pairs reduced the frequency of insertion into the hotspot by at least fivefold. The reduction in hotspot use caused by these G/C to A/T changes was not attributable to changes in plasmid supercoiling or tet promoter strength.. ...
The C-terminal domain of DNA gyrase A adopts a DNA-bending β-pinwheel fold - Fingerprint - Johns Hopkins University
Single-molecule imaging of DNA gyrase activity in living Escherichia coli<...
keywords = "Catalysis, DNA Gyrase/chemistry, DNA, Superhelical/chemistry, DNA-Binding Proteins/chemistry, Escherichia coli/ ... N2 - Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by ... AB - Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by ... Bacterial DNA gyrase introduces negative supercoils into chromosomal DNA and relaxes positive supercoils introduced by ...
Characteristics of compacted plasmid DNA by high pressurization.<...
Superhelical DNA Medicine & Life Sciences 15% * Dynamic Light Scattering Medicine & Life Sciences 13% ... For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded ... For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded ... For digestion by S1 nuclease, which selectively cleaves single strand DNA, the pressurized plasmid DNA was easily degraded ...
2.6: Structure and Function - Nucleic Acids (2023)
Why are there different topological forms of DNA? The answer is related to superhelical voltage and sequence distortion. ... DNA in bacteria. In bacteria, there is no nucleus for DNA. Instead, DNA is contained in a structure called a nucleoid (Figure ... Structure of DNA strands. Each DNA strand is assembled from dNTPs by the formation of a phosphodiester bond, catalyzed by DNA ... First, voltage can provide energy to "reverse" the structure of DNA. Z-DNA may emerge as a means of relaxation. Second, DNA can ...
Nucleosome - wikidoc
The nucleosome core particle (shown in the figure) consists of about 146[5] bp of DNA wrapped in 1.67 left-handed superhelical ... The nucleosome core particle consists of approximately 147[5] base pairs of DNA wrapped in 1.67 left-handed superhelical turns ... The DNA is non-uniformly bent and also contains twist defects. The twist of free B-form DNA in solution is 10.5 bp per turn, ... DNA site exposure. Work from the Widom laboratory has shown that nucleosomal DNA is in equilibrium between a wrapped and ...
Artiodactyla | Harvard Catalyst Profiles | Harvard Catalyst
Promoter prediction in E. coli based on SIDD profiles and Artificial Neural Networks | BMC Bioinformatics | Full Text
Recent studies have shown that DNA structural properties, such as curvature, stacking energy, and stress-induced duplex ... Promoter prediction and annotation of microbial genomes based on DNA sequence and structural responses to superhelical stress. ... DNA structural features are increasingly being recognized as an important tool for detecting promoters. Promoter prediction ... Wang H, Noordewier M, Benham CJ: Stress-induced DNA duplex destabilization (SIDD) in the E. coli genome: SIDD sites are closely ...
Mitochondrial Macromolecules Research Group | IBMB
Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat. Nature Structural and Molecular ... DNA Site-X and Site-Y, similar to and downstream from LSP, unexpectedly show distinct DNA-structure features and TFAM binds to ... Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat. Nature Structural and Molecular ... DNA specificities modulate the binding of human transcription factor A to mitochondrial DNA control region. Nucleic Acids ...