*  Timeline Nobels
... shared the prize for their work on green fluorescent protein, or GFP. Shimomura discovered the jellyfish protein in 1961. In ... Japan's Osamu Shimomura and Americans Martin Chalfie and Roger Tsien ... Stanley B. Prusiner, a neurologist from UC, won the Nobel Prize for his discovery of the new class of proteins called prions ... for discovering how receptor proteins carry signals from outside the human body into cells.. (SFC, 10/11/12, p.A1). 2012 Oct 11 ...
  http://timelines.ws/subjects/Nobels.HTML
*  Intravital Two-Photon Excitation Microscopy in Neuroscience: General Concepts and Applications | SpringerLink
... microscopy has revolutionized biomedical research during the last two decades by enabling high resolution fluorescent ... Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388(6645):882-887. doi: 10.1038/ ... Tsien R (1998) The green fluorescent protein. Annu Rev Biochem 67:509-544. doi: 10.1146/annurev.biochem.67.1.509 PubMedGoogle ... Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D (1994) Green fluorescent protein as a marker for gene expression. Science (New ...
  https://link.springer.com/chapter/10.1007/978-94-017-9361-2_1
*  Frontiers | Genetically encoded proton sensors reveal activity-dependent pH changes in neurons | Frontiers in Molecular...
Green fluorescent protein (GFP), derived from Aequorea victoria, has become one of the most popular fluorescent reporters for ... Miyawaki, A., Llopis, J., Heim, R., McCaffery, J. M., Adams, J. A., Ikurak, M., and Tsien, R. Y. (1997). Fluorescent indicators ... Bizzarri, R., Serresi, M., Luin, S., and Beltram, F. (2009). Green fluorescent protein based pH indicators for in vivo use: a ... Awaji, T., and Hirasawa, A. (2001). Novel green fluorescent protein-based ratiometric indicators for monitoring pH in defined ...
  https://www.frontiersin.org/articles/10.3389/fnmol.2012.00068/full
*  Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures - Current Protocols
... green fluorescent protein embryonic stem cells into neurons after co‐culture with auditory brain stem slices. Neuroscience, 162 ... Tsien, R. Y. (1998). The green fluorescent protein. Annual Review of Biochemistry, 67, 509-544. doi: 10.1146/annurev.biochem. ... Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature, 499, 295-300. doi: 10.1038/nature12354. ... Thomas, D., Tovey, S. C., Collins, T. J., Bootman, M. D., Berridge, M. J., & Lipp, P. (2000). A comparison of fluorescent Ca2+ ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-sc02d13.html?quicktabs_cp=toc
*  Nanomaterials in medicine and pharmaceuticals: nanoscale materials developed with less toxicity and more efficacy : European...
Tsien RY. The green fluorescent protein. Ann Rev Biochem 1998;67:509-44.CrossrefGoogle Scholar ... Some secrets of fluorescent proteins: distinct bleaching in various mouting fluids and photoactivation of cyan flourescent ... fluorescent proteins and luminescent transition metal complexes, have been widely used (10-15). Among them, fluorophores with ... Comparing to traditional fluorescent probes, they have shown many benefits including the free of auto-fluorescence background, ...
  https://www.degruyter.com/view/j/ejnm.2013.5.issue-2/ejnm-2013-0003/ejnm-2013-0003.xml
*  Aequoreidae - Wikipedia
Aequoreids include Aequorea victoria, the organism from which the green fluorescent protein gene was isolated. Only the polyp ... The full text Tsien, R. (1998). The green fluorescent protein. Annual Review of Biochemistry 67: 509-44. The full text. doi: ...
  https://en.wikipedia.org/wiki/Aequoreidae
*  Green fluorescent protein - Wikipedia
Roger Tsien on fluorescent proteins Excitation and emission spectra for various fluorescent proteins Green Fluorescent Protein ... The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green ... Protein tag pGLO Yellow fluorescent protein Genetically encoded voltage indicator Ormö M, Cubitt AB, Kallio K, Gross LA, Tsien ... Phillips GJ (Oct 2001). "Green fluorescent protein--a bright idea for the study of bacterial protein localization". FEMS ...
  https://en.wikipedia.org/wiki/Green_fluorescent_protein
*  Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor
Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-544. doi: 10.1146/annurev.biochem.67.1.509. [PubMed] [ ... Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in ... fluorescent protein; FRET: Förster resonance energy transfer; GFP: green fluorescent protein; IPTG: isopropyl-β-D- ... This biosensor named FluBO (fluorescent protein-based biosensor for oxygen) consists of the yellow fluorescent protein (YFP) ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3364895/
*  Optogenetic Reporters: Fluorescent Protein-Based Genetically-Encoded Indicators of Signaling and Metabolism in the Brain
Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-544. [PubMed] ... Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol ... Baird GS, Zacharias DA, Tsien RY. Circular permutation and receptor insertion within green fluorescent proteins. Proc Natl Acad ... Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature. 1997;388:882-887. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3494096/?lang=en-ca
*  Roger Y. Tsien - Wikipedia
Tsien RY (1997). "Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin". Nature. 388 (6645): 882- ... "the green fluorescent protein: discovery, expression and development." The multicolored fluorescent proteins developed in ... Typically, the gene coding for a protein of interest is fused with the gene for a fluorescent protein, which causes the protein ... "Improved green fluorescence" (PDF). Nature. 373 (6516): 663-4. doi:10.1038/373663b0. PMID 7854443. "Green Fluorescent Protein: ...
  https://en.wikipedia.org/wiki/Roger_Y._Tsien
*  Rapid Displacement of Vimentin Intermediate Filaments in Living Endothelial Cells Exposed to Flow | Circulation Research
Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-544. ... green fluorescent protein. Vascular endothelial cells (ECs) are highly specialized to respond to local changes in blood flow, ... Intermediate filaments in motion: observations of intermediate filaments in cells using green fluorescent protein-vimentin. Mol ... 1 15 16 the use of green fluorescent protein (GFP) now allows direct observation of spatiotemporal dynamics in living cells.17 ...
  http://circres.ahajournals.org/content/86/7/745?ijkey=07462f93eb7280af7d8ec1f97c1169f1769c57d8&keytype2=tf_ipsecsha
*  Real-time in vivo imaging of the beating heart at microscopic resolution
1. Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-544. [PubMed] ... Green fluorescent protein (GFP) cells were obtained from the bone marrow of ubiquitin-GFP mice. In these donor mice, the femur ... Green fluorescent protein (GFP)-expressing cells, freshly isolated from the bone marrow of donor mice, were subsequently ... 4. Tsien RY. Imagining imaging's future. Nat Rev Mol Cell Biol. 2003;(Suppl):SS16-21. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3622400/?lang=en-ca
*  Biliverdin - Wikipedia
"The Green Fluorescent Protein". Annual Review of Biochemistry. 67 (1): 509-544. doi:10.1146/annurev.biochem.67.1.509. PMID ... Rodriguez, Erik A.; Tran, Geraldine N.; Gross, Larry A.; Crisp, Jessica L.; Shu, Xiaokun; Lin, John Y.; Tsien, Roger Y. (2016- ... but green blood that gives terrestrial animals a green color seems to occur nearly exclusively in species that inhabit green ... In contrast to fluorescent proteins which form their chromophore through posttranslational modifications of the polypeptide ...
  https://en.wikipedia.org/wiki/Biliverdin
*  Polymerization of the SAM domain of TEL in leukemogenesis and transcriptional repression | The EMBO Journal
The TEL-SAM domain was fused to a variant of green fluorescent protein called cyan fluorescent protein (CFP) (Tsien, 1998). ... Waldo GS, Standish BM, Berendzen J and Terwilliger TC (1999) Rapid protein‐folding assay using green fluorescent protein. ... the protein was found in inclusion bodies and we were unable to refold the protein into a soluble form. Although proteins ... Thanos CD and Bowie JU (1999) p53 family members p63 and p73 are SAM domain‐containing proteins. Protein Sci, 8, 1708-1710. ...
  http://emboj.embopress.org/content/20/15/4173
*  Ionic determinants of pH of acidic compartments under hypertonic conditions in trout hepatocytes | Journal of Experimental...
... and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95,6803 -6808. ... Wu, M. M., Llopis, J., Adams, S., McCaffery, J. M., Kulomaa, M. S., Machen, T. E., Moore, H. P. and Tsien, R. Y. (2000). ... Wu, M. M., Grabe, M., Adams, S., Tsien, R. Y., Moore, H. P. and Machen, T. E. (2001). Mechanisms of pH regulation in the ... AO is a weakly basic dye that displays green fluorescence in the diluted monomeric form. As shown in Fig. 1, this dye ...
  http://jeb.biologists.org/content/211/20/3306
*  Membrane proximal lysosomes are the major vesicles responsible for calcium-dependent exocytosis in nonsecretory cells | JCB
Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad ... Llopis, J., J.M. McCaffery, A. Miyawaki, M.G. Farquhar, and R.Y. Tsien. 1998. ... The membrane was labeled by transiently expressing fluorescent reporter proteins including Synaptotagmin VII-EGFP (Martinez et ... GFP tagged proteins were excited with the 488-nm line of an Argon laser (Omnichrome, model 543-AP A01; Melles Griot) reflected ...
  http://jcb.rupress.org/content/159/4/625.long
*  Live cell imaging of mechanotransduction | Journal of The Royal Society Interface
Tsien R. Y.. 1997 Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887. (doi ... particularly green fluorescent protein (GFP) and its derivative fluorescent proteins (FPs), it is now convenient to visualize ... Tsien R. Y.. 2004 Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent ... 1994 Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. ...
  http://rsif.royalsocietypublishing.org/content/early/2010/03/29/rsif.2010.0042.focus
*  Molecules | Free Full-Text | Fluorescent Probes and Fluorescence (Microscopy) Techniques - Illuminating Biological and...
Green Fluorescent Protein. Because fluorescent proteins are synthesized in vivo, integration of fluorescent detection methods ... Currently fluorescent proteins are available that virtually span the whole electromagnetic spectrum. Concomitantly, ... Compounds that exhibit fluorescence are commonly called fluorochromes or fluorophores and one of these fluorescent molecules in ... Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 1998, 67, 509-544. [Google Scholar] ...
  http://www.mdpi.com/1420-3049/17/12/14067/htm
*  Oceans and Human Health - Oxford Research Encyclopedia of Environmental Science
... work on jellyfish fluorescent proteins led to the discovery of green fluorescent protein, one of the most widely used molecular ... Tsien, R.Y. (1998). The green fluorescent protein. Annual Review of Biochemistry, 67, 509-544.Find this resource: ... Growing appreciation of the importance of the built environment (e.g., positive effects of urban green and blue spaces on ... as an important source of protein and other nutrients (e.g., omega-3 fatty acids, which can contribute to heart health). More ...
  http://environmentalscience.oxfordre.com/view/10.1093/acrefore/9780199389414.001.0001/acrefore-9780199389414-e-12?rskey=wdcK6I&result=6
*  Patente US6936428 - Dimeric flourescent polypeptides - Google Patentes
... suited for fluorescent proteins and are particularly useful in applications in which more than one fluorescent protein sharing ... The invention also relates to nucleic acids encoding Intramolecular Dimer Fluorescent Proteins (IDFPs) and vectors comprising ... More specifically, the invention relates to recombinant polypeptides comprising a monomer of a fluorescent polypeptide, a ... linker peptide, and a second monomer of that fluorescent polypeptide, where the monomers form an intramolecular dimer. ...
  http://www.google.es/patents/US6936428?dq=flatulence
*  Synaptotagmin isoforms confer distinct activation kinetics and dynamics to chromaffin cell granules | JGP
Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74:1591-1599. doi:10.1016/S0006-3495(98) ... Tsien, 1998; Campbell and Choy, 2001).. The Syt-1 pHluorin/Syt-7 pHuji emission images were captured sequentially using ... The green fluorescent protein. Annu. Rev. Biochem. 67:509-544. doi:10.1146/annurev.biochem.67.1.509. ... The effect of pH on green fluorescent protein: a brief review. Mol. Biol. Today. 2:1-4. ...
  http://jgp.rupress.org/content/149/8/763
*  List of Asian Americans - Wikipedia
Roger Y. Tsien, 2008 Nobel Prize winner in chemistry, for the discovery of the green fluorescent protein. Daniel Tsui, 1998 ... in 1968 for work in genetics and protein synthesis. Tsung-Dao Lee, received the 1957 Nobel Laureate in Physics for work in ... American football linebacker for the Green Bay Packers. Bryan Clay - won the decathlon gold medal in the 2008 Olympics, the ... Killed in action during 1998 embassy bombing in Nairobi, Kenya Leandro Aragoncillo - Gunnery Sergeant, U.S. Marine Corps. ...
  https://en.wikipedia.org/wiki/List_of_Asian_Americans
*  Integrin Targeting for Tumor Optical Imaging
Tsien RY. The green fluorescent protein. Annu Rev Biochem. 1998;67:509-44 ... exogenous fluorescent molecules such as green fluorescent protein (GFP) or small synthetic optical contrast agents. Compared to ... fluorescent biological nanoparticles such as GFP and many other fluorescent proteins [19-20]. Among them, the NIR fluorescent ... Tsien RY. The 2009 Lindau Nobel Laureate Meeting: Roger Y. Tsien, Chemistry 2008. J Vis Exp. 2010(35 pii):1575 ...
  http://thno.org/v01p0102.htm
*  The renaissance of mitochondrial pH | JGP
Green fluorescent protein as a noninvasive intracellular pH indicator. Biophys. J. 74:1591-1599. doi:10.1016/S0006-3495(98) ... R.Y. Tsien. . 2008. Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat. Methods. 5:545- ... Mitochondrial pH monitored by a new engineered green fluorescent protein mutant. J. Biol. Chem. 279:11521-11529. doi:10.1074/ ... Structural and spectral response of green fluorescent protein variants to changes in pH. Biochemistry. 38:5296-5301. doi: ...
  http://jgp.rupress.org/content/139/6/415.full
*  Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice | BMC Biology | Full Text
Tsien RY: The green fluorescent protein. Annu Rev Biochem. 1998, 67: 509-544. 10.1146/annurev.biochem.67.1.509.View Article ... Arnone MI, Bogarad LD, Collazo A, Kirchhamer CV, Cameron RA, Rast JP, Gregorians A, Davidson EH: Green fluorescent protein in ... the green fluorescent protein (GFP) of Aequorea victoria and to a lesser degree human placental alkaline phosphatase [3, 5, 6 ... has been validated in mice by localizing stem cells using an enhanced yellow fluorescent protein targeted to the Lgr-5 locus ...
  https://preview-bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-8-89