*  The region 3′ to Xist mediates X chromosome counting and H3 Lys‐4 dimethylation within the Xist gene | The EMBO Journal
Heard E, Mongelard F, Arnaud D, Avner P (1999) Xist yeast artificial chromosome transgenes function as X‐inactivation centers ... Gartler SM, Riggs AD (1983) Mammalian X‐chromosome inactivation. Annu Rev Genet 17: 155-190. ... A counting process senses the X chromosome/autosome ratio and ensures that X chromosome inactivation (XCI) initiates in the ... Rastan S, Robertson EJ, Rasmussen TP, Mastrangelo MA, Eden A, Pehrson JR, Jaenisch R (1985) X‐chromosome deletions in embryo‐ ...
  http://emboj.embopress.org/content/23/3/594
*  KAKEN - Research Projects | Construction of mammalian artificial chromosomes using YAC (KAKENHI-PROJECT-08044200)
The purpose of this work is to clone functional human centromere DNA using technology of the yeast artificial chromosome (YAC ... transformed cell lines showed that transformants of alpha21-I YAC contained a stably maintained mammalian artificial chromosome ... Publications] T.Okazaki: 'Properties and interaction of CENP-B and centromere satellite DNA in mammalian cells.' Chromosome ... Publications] T.Okazaki: 'Properties and interaction of CENP-B and centromere satellite DNA in mammalian cells.' Chromosome ...
  https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044200/
*  Artificial chromosome - Patent # 6716608 - PatentGenius
... and further preparing a yeast strain in which mammalian telomere sequences are added to the ends of its chromosome. ... preparing a CEPH artificial yeast chromosome library containing a human genome, identifying clones having a repetitive human ... mammalian centromere sequence and a DNA replication origin with mammalian telomere sequences added to both ends of the clone, ... An artificial mammalian chromosome, more specifically, a clone containing a ...
  http://www.patentgenius.com/patent/6716608.html
*  Bacterial Artificial Chromosome Mutagenesis Using Recombineering
Baker A, Cotten M. Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus ... Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Kumaran Narayanan 1, 2 * and Qingwen Chen 2 ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ... Modification of bacterial artificial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice. [ ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3005948/
*  Bacterial artificial chromosomes improve recombinant protein production in mammalian cells | BMC Biotechnology | Full Text
In this work, we explore the efficacy of a Bacterial Artificial Chromosome based vector applied to production of the constant ... Blaas L, Musteanu M, Zenz R, Eferl R, Casanova E: PhiC31-mediated cassette exchange into a bacterial artificial chromosome. ... To overcome these chromatin effects, we have employed a Bacterial Artificial Chromosome (BAC) as expression vector to obtain ... Use of a Bacterial Artificial Chromosome (BAC) as vector backbone for recombinant protein production. A) Schematic ...
  https://bmcbiotechnol.biomedcentral.com/articles/10.1186/1472-6750-9-3
*  Application # 2018/0142210. MODIFIED STEM CELLS AND USES THEREOF - Patents.com
Other methods of gene delivery include use of mammalian artificial chromosomes (Vos, 1998, Curr. Op. Genet. Dev. 8:351-359); ... As a result, both the 5' and 3' LTRs will be inactivated when the provirus is produced and integrated into a chromosome. The ... In embodiments, the gene sequence encoding the sequences can be codon optimized for expression in mammalian cells. [0108] " ... chromosome-mediated gene transfer, microcell-mediated gene transfer, sheroplast fusion, etc. Numerous techniques are known in ...
  http://patents.com/us-20180142210.html
*  Bacterial artificial chromosome - Wikipedia
... vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery of infectious virus in mammalian ... Cosmid End-sequence profiling Fosmid Human artificial chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W ( ... A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for ... The bacterial artificial chromosome's usual insert size is 150-350 kbp. A similar cloning vector called a PAC has also been ...
  https://en.wikipedia.org/wiki/Bacterial_artificial_chromosome
*  Role of SV40 Integration Site at Chromosomal Interval 1q21.1 in Immortalized CRL2504 Cells
The transfer of a 150Kb bacterial artificial chromosome (BAC) clone, RP364B14, corresponding to viral integration site in ... Functional complementation by electroporation of human BACs into mammalian fibroblast cells. Nucleic Acids Res. 1998;26:1124-5. ... Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies. ... Fixed cells were transferred to glass slides to prepare metaphase chromosome spreads. The metaphase chromosome spreads were ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC2913896/
*  Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels
RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells. Proc Natl Acad Sci ... Homologous recombination in mammalian cells. Annu Rev Genet. 1989;23:199-225. [PubMed] ... Aurora B controls the association of condensin I but not condensin II with mitotic chromosomes. J Cell Sci. 2007;120:1245-1255. ... Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science. 1991;251:1351-1355. [PubMed] ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3605737/?lang=en-ca
*  US7816104B2 - Packaging cells for recombinant adenovirus - Google Patents
... bacterial artificial chromosome, and yeast artificial chromosome, said first nucleic acid molecule comprising an E1A coding ... Mammalian cell lines expressing bovine adenovirus functions WO2002040665A2 (en) 2000-11-15. 2002-05-23. Crucell Holland B.V.. ... bacterial artificial chromosome, and yeast artificial chromosome, said second nucleic acid molecule comprising an E1B coding ... bacterial artificial chromosome, yeast artificial chromosome, and recombinants thereof generated by homologous recombination, ...
  https://patents.google.com/patent/US7816104
*  Retrofitting BACs With a Selectable Marker for Transfection | Springer for Research & Development
... clones into mammalian cells. Because most BAC vectors do not have a mammalian selection marker or do not have... ... Many functional studies require the transfection of bacterial artificial chromosome (BAC) ... Many functional studies require the transfection of bacterial artificial chromosome (BAC) clones into mammalian cells. Because ... In: Zhao S., Stodolsky M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology, vol 256. Humana Press. * DOI ...
  https://rd.springer.com/protocol/10.1385/1-59259-753-X%3A069
*  Antibodies against human CSF-1R - HOFFMANN-LA ROCHE INC.
Jakobovits et al., "Germ-line Transmission and Expression of a Human-Derived Yeast Artificial Chromosome" Nature 362(6417):255- ... Schlaeger, E. et al., "The protein hydrolysate, primatone RL, is a cost-effective multiple growth promoter of mammalian cell ... Kaufman, R., "Overview of vector design for mammalian gene expression" J. of Molecular Biotechnology 16:151-161 ( 2000). King, ... Makrides, S., et al., "Components of Vectors for Gene Transfer and Expression in mammalian cells" Protein Expression and ...
  http://www.freepatentsonline.com/9169323.html
*  Interactive Fly, Drosophila
Genetic and physical mapping of scm identifies yeast artificial chromosomes containing an exon of mdab1, a homolog of ... The five known mammalian members of the family are the LDL receptor, LRP, Megalin, VLDLR, and ApoER2. The role of the LDL ... Formation of the mammalian brain requires choreographed migration of neurons to generate highly ordered laminar structures, ... During mammalian brain development, immature neurons migrate radially from the neuroectoderm to defined locations, giving rise ...
  http://www.sdbonline.org/sites/fly/cytoskel/disabl2b.htm
*  New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences ( One-column protein purification and n...)
Electroporation of Yeast Artificial Chromosomes. 4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting. 5 ... Mammalian Expression Vector for Efficient Cloning of PCR Fragments. 10. QIAGEN PCR CloningPlus Kit. 11. Cloning Based on ... Lu, Q., Jerpseth, B., Sanchez, T., Bauer, J.C., and Greener, A. (1997) Strategies 10: 4-6. ...
  http://www.bio-medicine.org/biology-technology/New-Yeast-Cloning-System-for-Producing-Proteins-with-Native-Amino-Acid-0D-0A-0ASequences-44-1/
*  Genetically Engineered Mice by Pronuclear DNA Microinjection - Current Protocols
Analysis of mammalian central nervous system gene expression and function using bacterial artificial chromosome‐mediated ... A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425:917‐925. ... Neuroendocrine aspects of mammalian reproduction. Annu. Rev. Physiol. 31:383‐416.. Finney, M. 2000. Pulsed‐field gel ... Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice. Nucleic Acids Res. 25:1009‐ ...
  http://www.currentprotocols.com/WileyCDA/CPUnit/refId-mo110168.html
*  Adding X and Y chromosomes to human cells
"Making CENs of mammalian artificial chromosomes", Mol Genet Metab, 1999, ( Abstract ). "Mammalian artificial chromosomes as ... you would have a human artificial chromosome - a HAC (more commonly referred to as mammalian artificial chromosome, MAC, but ... Human Artificial Chromosomes. Many years ago, scientists proposed a variety of approaches to making artificial chromosomes: ... Bibliography for Human Artificial Chromosomes. "Human artificial chromosome vectors meet stem cells: new prospects for gene ...
  http://samesexprocreation.com/mmct.htm
*  clone facts, information, pictures | Encyclopedia.com articles about clone
Bacterial artificial chromosomes (BACs) can contain up to 300 kb, and yeast artificial chromosomes (YACs), grown in yeast cells ... In a true mammalian clone (as in Gurdon's frog clone) the nucleus from a body cell of an animal is inserted into an egg, which ... If a section of DNA is engulfed into the chromosome of a bacterium, phage, or plasmid vector and is replicated to form many ... If a section of DNA is engulfed into the chromosome of a bacterium, phage, or plasmid vector and is replicated to form many ...
  https://www.encyclopedia.com/science-and-technology/biology-and-genetics/biology-general/clone
*  Plus it
The calbindin-D28K-bacterial artificial chromosome (BAC)::GFP transgenic mouse (termed here CalB::GFP; a gift from Dr. N. ... Best JD, Maywood ES, Smith KL, Hastings MH (1999) Rapid resetting of the mammalian circadian clock. J Neurosci 19: 828-835. ... GFP was inserted in the promoter region of calbindin-D28K-bacterial artificial chromosome (CalB::GFP mouse). In the latter ... Efficient studies of long-distance Bmp5 gene regulation using bacterial artificial chromosomes. Proc Natl Acad Sci USA 97: 1612 ...
  http://www.jneurosci.org/content/24/1/68
*  Plus it
1996) Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature ( ... 1991) Tissue culture of mammalian autonomic neurons. in Culturing nerve cells, eds Banker G, Goslin K (MIT, Cambridge, MA), pp ... 1996) Opposing motor activities are required for the organization of the mammalian mitotic spindle pole. J Cell Biol 135:399- ... 1997) FGF-2 induces nerve growth factor expression in cultured rat hippocampal neurons. Eur J Neurosci 9:1282-1289. ...
  http://www.jneurosci.org/content/18/19/7822
*  Delivery Techniques in Gene Therapy: A Brief Overview | OMICS International
Efficient in-vitro transfer of a 60-Mb mammalian artificial chromosome into murine and hamster cells using cationic lipids and ... Chromosome Res 9: 475-485.. *Schatzlein AG (2003) Targeting of Synthetic Gene Delivery Systems. J Biomed Biotechnol 2003: 149- ... The development of artificial viral systems (synthetic viruses) remains a future strategy to harness the advantages of viral ... chemical modification of viral vectors and synthesis of artificial virus vector systems. AAPS J 8: E731-742. ...
  https://www.omicsonline.org/open-access/delivery-techniques-in-gene-therapy-a-brief-overview-2161-0398.1000147.php?aid=28105
*  Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes | SpringerLink
... further been supported by groups using small-angle X-ray scattering and electron spectroscopic imaging of mammalian chromosomes ... 2011). In summary, cell cultures of strain RCC745 were grown in artificial seawater and were naturally synchronized to a 12-h ... Chromosome Res: Int J Mol Supramol Evol Asp Chromosome Biol 19:37-51CrossRefGoogle Scholar ... In chromosome 11, for example, there are 10.6 genes/megabase and genes are separated by an average of 86 kb (Taylor et al. 2006 ...
  https://link.springer.com/article/10.1007%2Fs00412-013-0423-z
*  Full text] Optogenetics: illuminating the neural bases of rodent behavior | OAAP
28 Bacterial artificial chromosome (BAC) transgenic or knock-in lines have been widely successful for expressing Cre or opsins ... Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013;500(7463):472-476. ... based modification in escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome. Nat ... Optogenetic interrogation of neural circuits: Technology for probing mammalian brain structures. Nat Protoc. 2010;5(3):439-456. ...
  https://www.dovepress.com/optogenetics-illuminating-the-neural-bases-of-rodent-behavior-peer-reviewed-fulltext-article-OAAP
*  Protein production method - Inter-University Research Institute Corporation Research Organization of Information and Systems
... into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and ... an artificial transposon Frog prince (Non-patent Reference 3) which is derived from frog and a transposon piggyBac (Non-patent ... into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing said protein of interest; and ... Illustratively, whether or not a non-automatic Tol2 element can be transferred and inserted into a mammalian cell chromosome by ...
  http://www.freepatentsonline.com/9034649.html
*  Serpentine transmembrane antigens expressed in human cancers and uses thereof - HUBERT RENE S.
... bacterial artificial chromosome libraries (BACs), yeast artificial chromosome libraries (YACs), and the like, with STEAP DNA ... STEAP function can be assessed in mammalian cells using in vitro approaches. For mammalian expression, STEAP can be cloned into ... while STEAP-2 and AI139607 localize to the long arm of chromosome 7, at 7q21 (. FIG. 17. ). R80991 maps to chromosome 2q14-q. ... 0372] Mammalian cell lines expressing STEAP can be tested in several in vitro and in vivo assays, including cell proliferation ...
  http://www.freepatentsonline.com/y2003/0149531.html
*  Patent US7074590 - Chimeric promoters - Google Patents
The chimeric promoter/enhancers are particularly suitable for directing gene expression in mammalian cells. ... a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC). Large numbers of suitable vectors and promoters ... a non-mammalian vertebrate, such as a bird (e.g., a chicken or duck) or a fish, and a non-mammalian invertebrate. ... a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC). ...
  http://www.google.co.uk/patents/US7074590