*  Human Genome Project facts, information, pictures | Encyclopedia.com articles about Human Genome Project
Cloning. Technically known as "somatic cell nuclear transfer," cloning techniques were developed in 1996 by Ian Wilmut at the ... Wilmut announced the cloning of Dolly the sheep in February 1997. The scientific breakthrough consisted of returning an already ... Defenders of nuclear transfer research distinguish sharply between reproductive cloning, which they oppose, and therapeutic ... It is likely that experiments with somatic cell nuclear transfer will be required to attain histocompatibility. Ethical ...
  https://www.encyclopedia.com/science-and-technology/biology-and-genetics/genetics-and-genetic-engineering/human-genome-project
*  Ian Wilmut - Wikipedia
However, in 2008 Wilmut announced that he would abandon the technique of somatic cell nuclear transfer by which Dolly was ... Wilmut was born in Hampton Lucy, Warwickshire, England. Wilmut's father, Leonard Wilmut, was a mathematics teacher who suffered ... In 1966, Wilmut spent 8 weeks working in the laboratory of Christopher Polge, who is credited with developing the technique of ... In 1997 Wilmut was Time magazine man of the year runner up. He was knighted in the 2008 New Year Honours. WILMUT, Sir Ian. ...
  https://en.wikipedia.org/wiki/Ian_Wilmut
*  Dolly (sheep) - Wikipedia
Wilmut, who led the team that created Dolly, announced in 2007 that the nuclear transfer technique may never be sufficiently ... She was created using the technique of somatic cell nuclear transfer, where the cell nucleus from an adult cell is transferred ... using the process of nuclear transfer. Dolly was cloned by Keith Campbell, Ian Wilmut and colleagues at the Roslin Institute, ... The reprogramming process that cells need to go through during cloning is not perfect and embryos produced by nuclear transfer ...
  https://en.wikipedia.org/wiki/Dolly_(sheep)
*  Circulation Research Thematic Synopsis | Circulation Research
... the success of the nuclear transfer technique demonstrated that the genome, even when isolated from adult cells, contains the ... cloned by Ian Wilmut and colleagues at the Roslin Institute in Scotland in July 1996.2 Although Dolly was euthanized in 2003 ... Cellular reprogramming achieved by somatic nuclear transfer or cell fusion has long been recognized. The potency of specific ... The convergence of these discoveries, namely somatic nuclear transfer, identification of "master" transcription factors, and ...
  http://circres.ahajournals.org/content/112/2/e14
*  HoofPrint - vol. 22 Winter2016 by HoofPrint- The Small Ruminant Magazine - issuu
The technique Keith Campbell, Ian Wilmut and their Roslin Institute colleagues used to create her is called nuclear transfer. ... Then in 1977, genes from other organisms were transferred to bacteria, an achievement that eventually led to the first transfer ... This technique continues to be used today and represents an early form of genetic engineering. The modern era of genetic ... It involves transferring the nucleus from a diploid cell (which contains the full complement of an individual's chromosomes) to ...
  https://issuu.com/hoofprintky/docs/hoofprintwinter2016
*  Advantages | Advantages of Domestic Species As Dual Purpose Models
Cloning & nuclear transfer: Ian Wilmut used nuclear transfer procedures to successfully clone the first mammal, a sheep named ... A history of farm animal embryo transfer and some associated techniques. Anim Reprod Sci 2003; 79: 203-244. ... Campbell KHS, McWhir J, Ritchie WA, Wilmut I. Sheep cloned by nuclear transfer from a cultured cell line. Nature 1996; 380: 64- ... Recently, nuclear transfer technology produced α-1,3-galactosyltransferase "knockout" pigs. Hearts and kidneys from these pigs ...
  http://adsbm.msu.edu/advantages
*  Zavos - Congress 2001
... double nuclear transfer' techniques may reduce the number of nuclear transplant embryos lost at this stage (Polejaeva et al ... The developmental efficiency of somatic cell nuclear transfer has slowly improved over the past five years. Dr. Wilmut achieved ... Porcine SCNT; 483 nuclear transfers. Independent experiments concluded that 8% (15/192) of nuclear transplants developed to G1 ... certain methods of nuclear transfer, such as electrofusion (Wilmut et al 1997), appear to be less efficient than microinjection ...
  http://zavos.org/library/library_congress01.htm
*  Dolly the Sheep and the human cloning debate - twenty years later
1996 to be precise. She was the first mammal to enter the world following a process of reproductive cloning… ... To create Dolly, researchers at the Roslin Institute in Scotland employed a technique known as somatic cell nuclear transfer ( ... The initial response to the dramatic Nature article by Wilmut et al. was largely one of fear, mingled with disgust, with too ... The result of SCNT is a creature with almost the same genetic potential as the one providing the nuclear DNA. SCNT is thus a ...
  http://theconversation.com/dolly-the-sheep-and-the-human-cloning-debate-twenty-years-later-63712
*  Somatic cell nuclear transfer - Wikipedia
Wilmut I, Beaujean N, de Sousa PA, et al. (October 2002). "Somatic cell nuclear transfer". Nature. 419 (6907): 583-6. doi: ... and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce ... Interspecies nuclear transfer (iSCNT) is a means of somatic cell nuclear transfer used to facilitate the rescue of endangered ... Nuclear Transfer - Stem Cells or Somatic Cell Nuclear Transfer (SCNT) The International Society for Stem Cell Research The ...
  https://en.wikipedia.org/wiki/Somatic_cell_nuclear_transfer
*  Cloning - Wikipedia
The modern cloning techniques involving nuclear transfer have been successfully performed on several species. Notable ... She was cloned at the Roslin Institute in Scotland by British scientists Sir Ian Wilmut and Keith Campbell and lived there from ... This may have important implications for cross-species nuclear transfer in which nuclear-mitochondrial incompatibilities may ... "somatic cell nuclear transfer" (SCNT) to create animals that are genetically identical. This process entails the transfer of a ...
  https://en.wikipedia.org/wiki/Cloning
*  List of English inventions and discoveries - Wikipedia
Dolly the Sheep born as a result of Nuclear transfer, a form of cloning put into practice by Ian Wilmut (born 1944) and Keith ... English inventions and discoveries are objects, processes or techniques invented, innovated or discovered, partially or ... 1932: Splitting the atom, a fully artificial nuclear reaction and nuclear transmutation, first achieved by English physicist ... a technique which has been widely used to identify the proteins encoded and to study their function. 1960 onwards: Pioneering ...
  https://en.wikipedia.org/wiki/List_of_English_inventions_and_discoveries
*  The Role of Biotechnology in Exploring and Protecting Agricultural Genetic Resources
Cloned zebrafish by nuclear transfer from long-term-cultured cells. Nature Biotechnology, 20: 795-799. ... animals or by the use of ovum pick-up techniques in live animals. The latter techniques are presently mainly in use in cattle ... Paterson, L., DeSousa, P., Ritchie, W., King, T. & Wilmut, I. 2003. Application of reproductive biotechnology in animals: ... 5.4.2 Embryo transfer. Surgical embryo transfer could be possible in principle in all mammalian livestock species. In contrast ...
  http://www.fao.org/docrep/009/a0399e/A0399E06.htm
*  Search | The Embryo Project Encyclopedia
Nuclear Magnetic Resonance Imaging to Visualize Fetal Abnormalities. Nuclear magnetic resonance imaging (MRI) is a technique to ... Nuclear transplantation, as it was first called, was later referred to as somatic nuclear transfer or cloning. ... Ian Wilmut (1944- ). British embryologist Sir Ian Wilmut, best known for his work in the field of animal genetic engineering ... Somatic Cell Nuclear Transfer in Mammals (1938-2013). In the second half of the. twentieth century, scientists learned how to ...
  http://embryo.asu.edu/search?text=nuclear%20transplantation
*  The generation of live offspring from vitrified oocytes - WRAP: Warwick Research Archive Portal
nuclear transfer from a cultured cell line. Nature 380: 64-66.. 7. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH (1997 ... techniques suitable for direct transfer. Theriogenology 43: 197-197.. 44. Abe Y, Hara K, Matsumoto H, Kobayashi J, Sasada H, et ... 4. Bowles EJ, Campbell KH, St John JC (2007) Nuclear transfer: preservation of a. nuclear genome at the expense of its ... It also offers the potential to store oocytes to rescue endangered species by somatic cell nuclear transfer and for the ...
  http://wrap.warwick.ac.uk/38798/
*  Indian Patents. 234335:'METHODS OF MODIFYING EUKARYOTIC CELLS'
3. Nuclear transfer from modified eukaryotic cells to oocytes to. generate cloned organisms with modified allele (Wakayama, et ... 1999; Wilmut, et al., Reprod Fertil Dev, 10:639-43,1998; Wilmut, et al., Nature,. 385:810-3,1997; Wakayama, et al., Nat Genet, ... Many of the techniques used to construct DNA vectors described herein are standard molecular biology techniques well known to ... 4. Cell-fusion to transfer the modified allele to another cell, including. transfer of engineered chromosome(s), and uses of ...
  http://www.allindianpatents.com/patents/234335-methods-of-modifying-eukaryotic-cells
*  Things scientists have cloned - GreenwichTime
Ian Wilmut's technique motivated many governments to ban research on human cloning. Dolly was later naturally mated and gave ... Dolly, right, the first cloned sheep produced through nuclear transfer from differentiated adult sheep cells, and Polly, the ... Dolly, right, the first cloned sheep produced through nuclear transfer from differentiated adult sheep cells, and Polly, the ... Dolly, right, the first cloned sheep produced through nuclear... Photo-4638204.62524 - GreenwichTime Image 5 of 27 ...
  http://www.greenwichtime.com/news/slideshow/Things-scientists-have-cloned-62524/photo-4638220.php
*  Clifton Emahiser's Watchman's Teaching Ministries
She was created using the technique of somatic cell nuclear transfer, where the cell nucleus from an adult cell is transferred ... This was done at Roslin Institute, part of the University of Edinburgh, Scotland by Ian Wilmut, Keith Campbell and colleagues ... "The technique used in swapping the genetic material was not immaculate: Some mutant DNA remained in the fertilized eggs and the ... The researchers swapped nuclear material in human eggs to create healthy embryos lacking disease-carrying mitochondrial DNA. ...
  https://emahiser.christogenea.org/node?page=1
*  Science In My Fiction » 2013 » December
... using traditional nuclear transfer, a technique now known as the Honolulu Technique. The nucleus was taken from the cumulus ... The team that created her, led by Scotsman Ian Wilmut, hoped to create an animal whose cells were genetically young again, ... pioneering the process of nuclear transfer. In this process, the nucleus is removed from an egg, and replaced with the nucleus ... Gurdon, J.B., & Byrne, J.A. (2003) The first half-century of nuclear transplantation. Proc. Natl. Acad. Sci. USA100, 8048-8052. ...
  http://scienceinmyfiction.com/2013/12/
*  Cloning
The modern cloning techniques involving nuclear transfer have been successfully performed on several species. Notable ... Campbell KH, McWhir J, Ritchie WA, Wilmut I (March 1996). "Sheep cloned by nuclear transfer from a cultured cell line". Nature ... Main article: Somatic-cell nuclear transfer. Somatic-cell nuclear transfer, known as SCNT, can also be used to create embryos ... This may have important implications for cross-species nuclear transfer in which nuclear-mitochondrial incompatibilities may ...
  https://readtiger.com/wkp/en/Cloning
*  转基因大动物常用载体频数分
14] WILMUT I, BEAUJEAN N, DE SOUSA P A, et al. Somatic cell nuclear transfer[J]. Nature, 2002, 419(6907):583-586.. [15] HOFMANN ... Research progress of transgenic animal preparation techniques and gene vector[J]. Acta Ecologae Animalis Domastici, 2011, 32(3 ... Study of transfer t-PA gene to the goat fetal fibroblasts and nuclear transfer[J].Journal of Northwest A&F University:Natural ... Nuclear transfer of goat somatic cells transgenic for humanlactoferrin[J]. Hereditas (Beijing), 2006, 28(12):1513-1519. (in ...
  http://manu22.magtech.com.cn/Jweb_xmsy/CN/abstract/abstract14506.shtml
*  Search | Page 6 | The Embryo Project Encyclopedia
Illmensee used nuclear transfer techniques (cloning) to create early mouse embryos from adult mouse cells, a technique ... Keith Campbell, Jim McWhir, William Ritchie, and Ian Wilmut cloned two sheep, Megan and Morag, using sheep embryo cells. The ... Dolly was the first mammal cloned from specialized adult (somatic) cells with the technique of somatic cell nuclear transfer ( ... Sheep Cloned by Nuclear Transfer from a Cultured Cell Line' (1996), by Keith Campbell, Jim McWhir, William Ritchie, and Ian ...
  https://embryo.asu.edu/search?text=in%20vitro&page=5
*  Keith Campbell (biologist) - Wikipedia
Campbell, K. & Wilmut, I. (1998) "Nuclear Transfer". Animal Breeding: Technology for the 21st Century. (ed: A.J. Clark; Harwood ... were successful in producing the world's first piglets by Somatic-cell nuclear transfer (SCNT), the so-called cloning technique ... link) Campbell, K. H. S. (1999). "Nuclear Equivalence, Nuclear Transfer, and the Cell Cycle". Cloning. 1 (1): 3-15. doi:10.1089 ... a team led by Ian Wilmut with Keith Campbell as the main (66% of the credit) contributor used the same technique and shocked ...
  https://en.wikipedia.org/wiki/Keith_Campbell_(biologist)
*  CSIRO PUBLISHING | Reproduction, Fertility and Development
Application of the zona-free manipulation technique to porcine somatic nuclear transfer. Cloning Stem Cells 3, 191-197. , ... Wilmut, I. , Beaujean, N. , de Sousa, P. A. , Dinnyes, A. , King, T. J. , Paterson, L. A. , Wells, D. N. , and Young, L. E. ( ... Shin, M. R. , Park, S. W. , Shim, H. , and Kim, N. H. (2002). Nuclear and microtubule reorganization in nuclear-transferred ... 2003). Nuclear remodelling and the developmental potential of nuclear transferred porcine oocytes under delayed-activated ...
  http://www.publish.csiro.au/rd/RD06089
*  Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer
Wilmut I, Beaujean N, de Sousa PA, Dinnyes A, King TJ, Paterson LA, Wells DN, Young LE. Somatic cell nuclear transfer. Nature. ... Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. ... Campbell KH, McWhir J, Ritchie WA, Wilmut I. Sheep cloned by nuclear transfer from a cultured cell line. Nature. 1996;380:64-66 ... by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer (NT ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3772789/?lang=en-ca
*  House of Brungardt: 2007
Of course, trying nuclear transfer with human cells is harder. Human eggs are in short supply; the technology is tricky and ... Wilmut. "It seems we should all focus our efforts on reprogramming.". Still, many scientists want reprogramming techniques to ... However, the nuclear transfer approach - which uses a cloning step to get embryonic stem cells - isn't likely to disappear. ... many more people will move from nuclear transfer to this method." ...
  http://houseofbrungardt.blogspot.com/2007/