Mistletoe (Viscum album) | Plant Profiler | Sigma-Aldrich
Stein, G. M. and Berg, P. A. Flow cytometric analyses of the specific activation of peripheral blood mononuclear cells from ... on the cytotoxicity of an aqueous mistletoe drug and of purified mistletoe lectins tested on human T-leukemia cells. ... Immunologic effects: Subcutaneous mistletoe extract (ABNOBAviscum Mali, AM) therapy caused a strong proliferation of peripheral ... Cytotoxicity may also occur as a result of lectins damaging cell membranes with an influx of Ca2+ and cell shrinkage.5 In tumor ...https://www.sigmaaldrich.com/life-science/nutrition-research/learning-center/plant-profiler/viscum-album.html
"Detection of major histocompatibility complex class I-restricted, HIV-" by Richard A. Koup, John L. Sullivan et al.
The responses were resistant to natural killer (NK) cell depletion and were inhibited by monoclonal antibodies (MoAbs) to the T ... Using a system of autologous B and T lymphoblastoid cell lines infected with recombinant vaccinia vectors (VVs) expressing HIV- ... cell receptor, CD8 surface antigens, and MHC class I antigens, suggesting a classical MHC class I restricted, virus-specific ... were detected in the peripheral blood mononuclear cells (PBMCs) of HIV-1-infected individuals. ...http://escholarship.umassmed.edu/oapubs/309/
Frontiers | FCγ Chimeric Receptor-Engineered T Cells: Methodology, Advantages, Limitations, and Clinical Relevance | Immunology
CR T cells mediate anti-cancer activity in vitro and in vivo by an antibody-mediated-cellular-cytotoxicity (ADCC) mechanism. ... of immunologic strategies to enhance the ability of a patient's immune system to recognize and eliminate malignant cells. ... Lastly, we compare the advantages and limitations of the CD16-CR T cell technology with those of CAR T cell methodology. ... First, we review the current state-of-the-art of pre-clinical CD16-CR T cell technology. Second, we describe its in vitro and ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00457/full
Chronic Illness Report 6
Altered cytokine release in peripheral blood mononuclear cell cultures from patients with the chronic fatigue syndrome. ... technologic differences among the immunologic tests applied. Overall, results from 124 PVFS patients were summarized. The ... Sex-related differences in the pattern of Coxsackievirus B-3-induced immune cell cytotoxicity against virus-infected myofibers ... Peripheral blood mononuclear cells were assayed for alpha interferon production. The authors report that children with PVFS ...http://meactionuk.org.uk/Chronic_Illness_Report_6.htm
Serum Immune Proteins in Moderate and Severe Chronic Fatigue Syndrome/Myalgic Encephalomyelitis Patients
Altered cytokine release in peripheral blood mononuclear cell cultures from patients with the chronic fatigue syndrome. ... ADCC: antibody-dependent cell-mediated cytotoxicity; ANOVA: analysis of variance test; CFS/ME: Chronic Fatigue Syndrome/Myalgic ... Cytokine and other immunologic markers in chronic fatigue syndrome and their relation to neuropsychological factors. Applied ... NK cells also produce increased amounts of IL-8 which may lead to the attraction of T cells, B cells and other NK cells [31, 33 ...http://www.medsci.org/v12p0764.htm
ASMscience | T-Cell Responses and Cyt
... for the Th1 cytokines gamma interferon and IL-2 is greater in pleural fluid mononuclear cells than in blood mononuclear cells, ... manifested by a high frequency of negative tuberculin skin tests and failure of T lymphocytes to proliferate in vitro in ... Published data on cytokine production by human T cells in response to M. tuberculosis are conflicting. In patients with ... More comprehensive studies are needed to assess the role of CD8+ cells in human antituberculosis defenses. One strategy for ...http://www.asmscience.org/content/book/10.1128/9781555818357.chap25
Proliferation and 1/2 Cytokine Production in Human Peripheral Blood Mononuclear Cells after Treatment with Cypermethrin and...
Preliminary cytotoxicity studies were performed using PBMC from 2 donors/test agent to assess biovariance. PBMC (105 cells/well ... In those studies, significant effects on hemato-/immunologic parameters were seen with mancozeb doses that ranged from 250 to ... The final cell pellet containing peripheral blood mononuclear cells (PBMC) was resuspended to a final level of 1-2 × 106 cells/ ... Peripheral Blood Mononuclear Cells. Upon obtaining informed consent, peripheral blood was collected from individual male ...https://www.hindawi.com/journals/jt/2014/308286/
HLA-Mismatched Renal Transplantation without Maintenance Immunosuppression
Mixed lymphocyte reaction and cell-mediated lympholysis assays of the recipient's peripheral-blood mononuclear cells were ... The T cells from these four recipients, tested in vitro, showed donor-specific unresponsiveness and in specimens from allograft ... 16 Direct cross-match of recipient serum on donor lymphocytes was performed by measurement of cytotoxicity, flow-cytometric ... In Vitro Immunologic Assays. ... Multilineage blood-cell chimerism was detectable in all five ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2819046/
Interferon-Tau has Antiproliferative effects, Represses the Expression of E6 and E7 Oncogenes, Induces Apoptosis in Cell Lines...
Expression of the antiviral protein MX in peripheral blood mononuclear cells of pregnant and bred, non-pregnant ewes. J ... dendritic cell to the antigen-presenting to establish an intrinsic cellular immunologic response against autologous tumor cell ... test was used for the expression of E6 and E7. To analyzed the difference in tumor volume an ANOVA test was performed. In both ... other classes of IFNs have garnered attention due to their therapeutic potential and comparatively lower cytotoxicity at high ...http://www.jcancer.org/v07p2231.htm
Increased Populations of Regulatory T Cells in Peripheral Blood and Tumor-Infiltrating Lymphocytes in Patients with Gastric and...
... cells were purified from peripheral blood mononuclear cells with magnetic beads. The cytokine production [interleukin (IL)-10 ... Sakaguchi S., Sakaguchi N., Asano M., Itoh M., Toda M. Immunologic self-tolerance maintained by activated T cells expressing IL ... and natural killer cell-mediated cytotoxicity. Eur. J. Immunol., 26: 1308-1313, 1996. ... Differences between the values were determined using Student's t test. Significance was determined as P , 0.05. ...http://clincancerres.aacrjournals.org/content/9/12/4404.long
Upregulation of phagocyte-derived catecholamines augments the acute inflammatory response.
Following our recent report that phagocytic cells (neutrophils, PMNs, and macrophages) are newly discovered sources of ... 1999;Endogenous catecholamine synthesis, metabolism storage, and uptake in human peripheral blood mononuclear cells.Exp Hematol ... 2001;Physiological concentrations of dopamine inhibit the proliferation and cytotoxicity of human CD4+ and CD8+ T cells in ... Data were analyzed with a one-way ANOVA and individual group means were then compared with a Student-Newman-Keuls test. ...http://www.biomedsearch.com/nih/Upregulation-phagocyte-derived-catecholamines-augments/19212441.html
HER2 Expression Beyond Breast Cancer: Therapeutic Implications for Gynecologic Malignancies
... trastuzumab showed a four-fold increase in antibody-dependent lytic activity from isolated peripheral blood mononuclear cells ... and the combination of both in cell lines expressing high levels of HER2. Significant cytotoxicity was achieved with pertuzumab ... in 2007 published recommendations for optimal HER2 testing performance. This new testing algorithm relies on accurate and ... The mechanism of tumor aggressiveness includes resistance to immunologic mediators such as tumor necrosis factor (TNF-α), ...http://pubmedcentralcanada.ca/pmcc/articles/PMC3660991/?lang=en-ca
Effect of Tai Chi on mononuclear cell functions in patients with non-small cell lung cancer | BMC Complementary and Alternative...
... exercise on the proliferative and cytolytic/tumoricidal activities of peripheral blood mononuclear cells (PBMCs) in ... Analysis of NKT cells, NK cells, and CD123+ and CD11c + dendritic cells were also performed. (1) After 16-week of TCC, cell ... PBMC proliferation and co-culture of PBMCs with the NSCLC cell line A549 were performed for proliferation and cell cytolysis ... 3) Significant differences were also found in NK cell percentage at t = 16 weeks, post-pre changes of NKT and DC11c between ...https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-015-0517-7
Cytotoxicity Assays.. For analysis of cytotoxic T cells, blood samples (100 ml before first vaccination, 50 ml at days 28, 56, ... Abstract B18: Quantification of BTK engagement by ibrutinib in peripheral blood mononuclear cells in a phase I clinical study. ... reacted in the delayed type of hypersensitivity test and had sufficient numbers of CD4 and CD8 cells but may be due to T-cell ... Chee Y. H., Namkoong S. E., Kim D. H., Kim S. J., Park J. S. Immunologic diagnosis and monitoring of cervical cancers using in ...http://clincancerres.aacrjournals.org/content/8/12/3676
Biobehavioral Factors in Health and Disease - Health and Behavior - NCBI Bookshelf
People who were tested after surviving Hurricane Andrew had lower NKCC and fewer suppressor T cells (CD8+) and helper T cells ( ... These low-molecular-weight products of mononuclear cells are secreted during inflammatory and immune responses. They were shown ... Higher blood pressures of urban migrants from an African low-blood pressure population are not due to selective migration. ... as indicated by lower natural killer-cell cytotoxicity (NKCC) and lower numbers of circulating T lymphocytes. ...https://www.ncbi.nlm.nih.gov/books/NBK43737/
Mononuclear cells modulate the activity of pancreatic stellate cells which in turn promote fibrosis and inflammation in chronic...
Primary human blood mononuclear cells (PBMC) and PSC were cocultured, followed by analysis of cytokines and extracellular ... 0.05 as assessed using the Kruskal-Wallis test followed by Dunn's post-hoc test to compare single-cell PSC with cocultured PSC. ... Cytotoxicity and immunological incompatibilities were excluded due to spatial distance between the cells in the co-culture ... Our current results underline the change in PSC phenotype towards a more fibroblast-like cell type with immunologic function. ...https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-5-63
ASMscience | Functions of Antibodies
In addition, in vivo models, particularly those engineered to knock in or knock out effector cells or effector molecules, are ... Antibodies can impact pathogens in the presence or in the absence of effector cells or effector molecules such as complement, ... Increased susceptibility of malaria-infected variant erythrocytes to the mononuclear phagocyte system. Blood Cells 16:591-597. ... Microneutralization test for influenza A and B and parainfluenza 1 and 2 viruses that uses continuous cell lines and fresh ...http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.AID-0019-2014
A Pilot Study of Immune and Mood Outcomes of a Community-Based Intervention for Dementia Caregivers: The PLST Intervention
T-Cell Proliferation to ConA and PHA T-cell proliferative response of peripheral blood mononuclear cells to the mitogens PHA ( ... Natural Killer (NK) Cell Cytotoxicity NK cell cytotoxicity was determined by a 4-hour microtiter 51Chromium (51Cr) release ... Immunologic studies of prostatic cancer using the R3327 rat model. Transactions of the American Association of GU Surgeons. ... Independent sample t-tests were conducted on all demographic, health, and outcome variables to determine if groups were ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2864067/
PD-L1 on tumor cells is sufficient for immune evasion in immunogenic tumors and inhibits CD8 T cell cytotoxicity | JEM
T cell cytotoxicity, we next directly tested whether PD-L1 on MC38 cells can suppress CD8+ T cell cytotoxicity. To do so, we ... B7-H1 is a ubiquitous antiapoptotic receptor on cancer cells. Blood. 111:3635-3643. doi:10.1182/blood-2007-11-123141. ... tumors were passed through 70-µm filters and mononuclear cells isolated by centrifugation through a Percoll gradient (40 and 70 ... Combination therapy with anti-CTLA-4 and anti-PD-1 leads to distinct immunologic changes in vivo. J. Immunol. 194:950-959. doi: ...http://jem.rupress.org/content/214/4/895
To determine the cell cycle distribution, frozen or freshly prepared mononuclear cells of leukemic samples (0.5× 106-1 × 106) ... Mann-Whitney U test; Fig. 4A ). We next aimed at correlating the increase in S and G2-M phases with the increase in cell cycle ... Genetic and immunologic subclasses described by gene expression profiling studies of initial ALL (10, 12) were correctly ... Fifty-seven bone marrow-derived samples and 3 peripheral blood-derived samples with a mean blast count of 84% (minimum, 60%; ...http://clincancerres.aacrjournals.org/content/12/15/4553
Immunology and Pregnancy Losses: HLA, Autoantibodies and Cellular Immunity - Madame Curie Bioscience Database - NCBI Bookshelf
... of placenta from women with recurrent pregnancy losses of immune etiologies often demonstrate increased inflammatory cell ... It has been postulated that immunologic aberrations may be the cause in many of such cases. Immunopathological evaluation ... increased peripheral blood NK cell cytotoxicity has predictive value for pregnancy losses.84,87 Thus, conventional NK cells and ... CD16-CD56bright NK cells that are isolated from total decidual mononuclear cells at the early stage of pregnancy produce many ...https://www.ncbi.nlm.nih.gov/books/NBK6615/
The 'immunologic advantage' of HIV-exposed seronegative indi... : AIDS
Risk associated HIV-1 cross-clade resistance of whole peripheral blood mononuclear cells from exposed uninfected individuals ... activate complement-mediated cell lysis, induce antibody-dependent cell-mediated cytotoxicity (ADCC) [86-88], and inhibit ... HLA antibodies in blood donors with reactive screening tests for antibody to the immunodeficiency virus. Transfusion 1987; 27: ... Increased susceptibility to HIV-1 infection of peripheral blood mononuclear cells from chronically immune-activated individuals ...https://journals.lww.com/aidsonline/fulltext/2009/01140/the__immunologic_advantage__of_hiv_exposed.2.aspx
Philadelphia, PA: W.B. Saunders Co; Effects of larch arabinogalactan on human peripheral blood mononuclear cells: resultsfrom ... from cell cultures of the plant Echinacea purpurea to test subjects mediates activation of and Achillea species. Planta Med ... The role of Echinacea and larch Immunologic in vivo and in vitro studies on arabinogalactan to stimulate the specific and non- ... Mechanism of stimula- tion of human natural killer cytotoxicity by arabinogalactan from Larix occidentalis.. MOS 36-Item Short- ...http://healthdrugpdf.com/o/optox.it1.html
Patent US6039684 - Non-lethal conditioning methods for the treatment of acquired ... - Google Patents
While long-term engraftment of donor cells was not observed, the non-lethal preparative conditioning regimen was able to reduce ... was conditioned by non-lethal doses of irradiation and a chemotherapeutic drug prior to receiving donor bone marrow cells from ... T cell subsets (CD4, CD8), HIV-1 RNA (bDNA, NASBA), peripheral blood mononuclear cell (PBMC) co-culture and mixed lymphocyte ... Peripheral blood was obtained from the patient two weeks prior to transplant. The cells were tested for reactivity in both MLR ...http://www.google.com/patents/US6039684?dq=Donato+M.+Fraioli
A Collaborative Study on Immune Response in Sarcomas
peripheral blood mononuclear cells. *TcR repertoire in patients who have received immune checkpoint inhibitors ... Antibody-dependent cell lysis by NK cells is preserved after sarcoma-induced inhibition of NK cell cytotoxicity. Cancer Immunol ... To test our hypothesis, we will focus especially on two aneuploid sarcomas: Undifferentiated Pleomorphic Sarcoma and Osteogenic ... Aim 1. Assessment and validation of immunologic biomarkers and immune system infiltration in specific sarcoma subtypes. This ...http://www.sarcomahelp.org/immunosarc.html