Data analysis. The goal of the microarray analysis was to identify specific antibodies that can be used to differentiate ... For immunohistochemistry (IHC) experiments, independent tissue samples were used. All tissue samples (n = 20) had both DCIS and ... Two representative arrays showing the regular pattern of spotted control proteins (left panel, subarray incubated with buffer ... 1A). As expected, all controls (Alexa Fluor 647-conjugated antibody, anti-human IgG antibody and human IgG) were highly ...http://clincancerres.aacrjournals.org/content/18/7/1992
Frozen tissue array - Wikipedia
Consequently frozen tissue array should be the best tool for high throughput analysis on this purpose. Frozen tissue cores with ... especially analyze different protein levels with antibodies by immunohistochemistry. Various paraffin tissue arrays are now ... Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to ... Tissue microarrays from frozen tissues-OCT technique. Methods Mol Biol. 2010, 664:73-80. "Frozen Tissue Array". BioChain ...https://en.wikipedia.org/wiki/Frozen_tissue_array
Tissue microarray - Wikipedia
Tissue microarrays are particularly useful in analysis of cancer samples. One variation is a Frozen tissue array. The use of ... Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest 1986, 55: ... Tissue microarrays (also TMAs) consist of paraffin blocks in which up to 1000 separate tissue cores are assembled in array ... These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Sections from this ...https://en.wikipedia.org/wiki/Tissue_microarray
Ciphergen ProteinChip array. On each spot of PS1 series ProteinChip array, 1 μg of purified 6E10 monoclonal antibody (Signet ... Immunoblot analysis and ELISA. Mouse tissues were dissected and homogenized in T-PER buffer (Pierce, Rockford, IL) in the ... As control, the same membrane was stripped and blotted with β3-tubulin antibodies. BACE1+/+ tissue from the following: 1, ... For BACE1 immunostaining, an IgG purified anti-BACE1 fusion protein antibody was applied (1 μg/μl) as primary antibody in Tris- ...http://www.jneurosci.org/content/25/50/11693
After analysis, we first excluded all probe sets that showed no change in expression between the two arrays and those that were ... Tissue was homogenized on ice using a ground glass homogenizer. The routine Trizol protocol was modified to deal with tissue of ... C and detected with Texas Red-conjugated goat anti-rabbit antibody (Ab) (4 μg/ml; Invitrogen, Eugene, OR). In some experiments ... Scanning and data analysis.. MOE430A arrays were scanned on the GeneChip Scanner 3000 and analyzed using Affymetrix GeneChip ...http://www.jneurosci.org/content/26/48/12576
MolBiol.ru || ??
Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Mix-n-Stain Antibody Labeling Kits. : A revolutionary protein labeling technology that allows anyone to optimally label an ... and Tissue MicroArrays. Markers & Electrophoresis Reagents. miRNA (microRNA) Studies. Northern Analysis. Nuclease Control. ... DNA-binding Dye: This is the only dye that works perfectly for both qPCR & sensitive melt curve analysis. CF dyes. : A series ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&bb_products=60
BACE1, a Major Determinant of Selective Vulnerability of the Brain to Amyloid-β Amyloidogenesis, is Essential for Cognitive,...
Ciphergen ProteinChip array. On each spot of PS1 series ProteinChip array, 1 μg of purified 6E10 monoclonal antibody (Signet ... Immunoblot analysis and ELISA. Mouse tissues were dissected and homogenized in T-PER buffer (Pierce, Rockford, IL) in the ... For BACE1 immunostaining, an IgG purified anti-BACE1 fusion protein antibody was applied (1 μg/μl) as primary antibody in Tris- ... ProteinChip Array, and Aβ ELISA analyses. Equal amounts of lysates were subject to SDS-PAGE (Tris- glycine mini gel; Invitrogen ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2564291/?lang=en-ca
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity | Diabetes
Analysis of expression arrays.. Differences in WAT expression of mRNAs/miRNAs between obese/nonobese subjects and during ... Primary antibodies detecting MYC-associated factor X (MAX) were unspecific and, therefore, could not be used. ... A.K., M.C., S.L.-C., A.E., J.L., P.H., and J.-F.T. collected tissue and performed the functional analyses. P.J.B., M.P., K.D.-W ... M.R. collected tissue, performed the functional analyses, and wrote the manuscript. P.A. designed the study and wrote the ...http://diabetes.diabetesjournals.org/content/61/8/1986.full
Alternative splicing - Wikipedia
... arrays from ExonHit or Jivan) have been used. The array is then probed with labeled cDNA from tissues of interest. The probe ... In microarray analysis, arrays of DNA fragments representing individual exons (e.g. Affymetrix exon microarray) or exon/exon ... A trans-acting splicing regulatory protein of interest is then precipitated using specific antibodies. When the RNA attached to ... Most EST libraries come from a very limited number of tissues, so tissue-specific splice variants are likely to be missed in ...https://en.wikipedia.org/wiki/Alternative_splicing
Frontiers | Effect of P2X4 and P2X7 receptor antagonism on the pressure diuresis relationship in rats | Physiology
Statistical analysis was performed by Mann-Whitney U-test (for Western analysis) or by unpaired t-test (physiological data). ... The P2X4 antibody detected a band of ~60 kDa; the P2X7 antibody detect a band at ~75 kDa. The autoradiogram was scanned and ... The 16 CEL intensity files were imported into Bioconductor and arrays normalized by the Robust Multi-array Average (RMA) method ... They were not tissue specific and assumed no mutual exclusivity with inflammatory, or other disease, processes. The Endeavour ...https://www.frontiersin.org/articles/10.3389/fphys.2013.00305/full
Nuclear pore complex proteins mark the implantation window in human endometrium | Journal of Cell Science
Z.N.B. performed tissue array analyses. A.J.P. and S.M.K. collected site 1 biopsies. N.S. coordinated biopsy collection and, ... Antibodies. Mouse IgGs (Covance Research Products, Princeton, NJ) of mAb414 (Davis and Blobel, 1986) were used at 2 μg/ml for ... 4B). Moreover, analysis of tissue arrays containing six paraffin sections each of human esophagus, stomach, liver, colon, ... Tissue arrays used were 59 endometrial carcinomas (adenocarcinomas grade I-III) (Cybrdi, Frederick, MD), normal tissue of ...http://jcs.biologists.org/content/121/12/2037
A randomised controlled phase II trial of pre-operative celecoxib treatment reveals anti-tumour transcriptional response in...
Images of the arrays were quantified with GCOS software (Affymetrix). The chip description file (CDF) used for the analysis was ... Chalkley H: Method for the quantitative morphologic analysis of tissues. J Natl Cancer Inst. 1943, 4: 47-53.Google Scholar ... Cell proliferation was assessed using the MIB1 mouse monoclonal antibody to Ki-67 . Measurement of apoptosis was performed ... KK provided surgical support in tissue acquisition and critically revised the manuscript. TA performed microarray analyses and ...https://rd.springer.com/article/10.1186/bcr3409
Patent US7092557 - Method and device for selectively targeting cells within a three-dimensional ... - Google Patents
For example, four populations of primary prostate cells are purified for mRNA analysis by binding antibodies against specific ... and DNA array comparison of normal and cancerous tissues have been used to investigate the distinct patterns of gene expression ... For example, one antibody labeled with phycoerythrin (PE) and another antibody labeled with Texas Red could be used to identify ... Cell Analysis Systems, Inc.. Method and apparatus for automated analysis of biological specimens. ...http://www.google.com/patents/US7092557?dq=patent:+7360079
Patent US6642018 - Method for inducing a response in one or more targeted cells - Google Patents
For example, four populations of primary prostate cells are purified for mRNA analysis by binding antibodies against specific ... and DNA array comparison of normal and cancerous tissues have been used to investigate the distinct patterns of gene expression ... For example, one antibody labeled with phycoerythrin (PE) and another antibody labeled with Texas Red could be used to identify ... Tissue Engineering. In another application, the present apparatus is used to remove contaminating cells in inocula for tissue ...http://www.google.com/patents/US6642018?dq=6175559
US8283158B2 - Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two dimensional...
... tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols. ... relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, ... is employed to facilitate the amplification and/or other analysis of biomolecules arrayed on a gel, tissue sample, membrane, ... Sanchez et al., "Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots", ...https://patents.google.com/patent/US8283158
Aberrant STYK1 expression in ovarian cancer tissues and cell lines
An ovarian tissue array (Pantomics, Inc.) was stained with STYK1 antibody. (A) Each tissue section was assigned a score of 0 ... Statistical analysis. Statistical analyses were performed using the one-way ANOVA test in GraphPad Prism (San Diego, CA). The ... Tissue panel and immunohistochemistry. Formalin-fixed arrays of normal, benign, and malignant ovarian tissues were obtained ... Antibodies. STYK1 and GPR30 antibodies were purchased from AbCam (Cambridge, MA). α-Tubulin antibody was purchased from ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2773766/
RKIP phosphorylation and STAT3 activation is inhibited by oxaliplatin and camptothecin and are associated with poor prognosis...
Samples were divided and either saved for Western blot analysis (input) or incubated with an antibody to gp130 for 6 h. Protein ... All tissue samples were formalin fixed and paraffin embedded. The corresponding H&E slides were reviewed for confirmation of ... Three to six cores of tumor were arrayed per specimen. In addition, a core of normal adjacent colonic mucosa was also sampled ... Statistical analysis. Chi-square analysis was used to evaluate the association between STAT3 expression and tumor grade and ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-463
MolBiol.ru || ??
Cell and tissue culturing in normoxia and hypoxia. - Expression and secretion analysis. - Other methods of molecular and cell ... Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Antibody production in a wide range of animal models as well as Peptide-, Antibody- and ProteinArrays are also amongst its ... Multiplex Suspension Arrays Bio-Plex System and Arrays. / ?? , #13308 /. Bio-Rad Laboratories Headquarters 1000 Alfred Nobel ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&bb_location=12
Patent US6043053 - Wnt-3 polynucleotides - Google Patents
"Antibodies" as used herein includes polyclonal and monoclonal antibodies, chimeric, single chain, and humanized antibodies, as ... Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and ... Array technology methods are well known and have general applicability and can be used to address a variety of questions in ... Nucleic acids for diagnosis may be obtained from a subject's cells, such as from blood, urine, saliva, tissue biopsy or autopsy ...http://www.google.com/patents/US6043053
US7544471B2 - Preparing RNA from a wax-embedded tissue specimen - Google Patents
Methods of performing an array analysis of a labeled RNA sample are also described. ... a wax-embedded tissue specimen is digested and the resulting digested tissue specimen is purified to provide a purified RNA ... Representative capture agents include polypeptides and polynucleotides, for example antibodies, peptides, or fragments of ... A method of performing an array analysis comprising: labeling a plurality of miRNAs from a wax-embedded tissue specimen using a ...https://patents.google.com/patent/US7544471?oq=7%2C346%2C545
Protocols and Video Articles Authored by André Baruchel
... a genome-wide copy-number analysis by array-based comparative genomic hybridization (array-CGH) identified short somatic ... Ovarian tissue cryopreservation (OTC) may make it possible for survivors of childhood cancer to have children. We evaluated the ... Humoral response was assessed by H1N1-specific antibody titration. IL2 and IFNγ responses to influenza were also evaluated by ... Pangenomic analysis by SNP-array showed no abnormality at these loci. Three frameshift mutations of ASXL1 leading to a ...https://www.jove.com/author/Andr%C3%A9_Baruchel
MolBiol.ru || ??
Cell and tissue culturing in normoxia and hypoxia. - Expression and secretion analysis. - Other methods of molecular and cell ... Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Mix-n-Stain Antibody Labeling Kits. : A revolutionary protein labeling technology that allows anyone to optimally label an ... Multiplex Suspension Arrays Bio-Plex System and Arrays. / ?? , #13308 /. Bio-Rad Laboratories Headquarters 1000 Alfred Nobel ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&bb_location=9
Brevet US7615350 - Methods for haplotyping genomic DNA - Google Brevets
This linkage analysis is particularly useful when HLA typing for an individual with limited family HLA typing available. ... The genomic DNA is contacted and hybridized to the arrayed oligonucleotides to form a genomic DNA/oligonucleotide complex. The ... Separation of the haplotypes of large genomic DNA fragments allows for linkage analysis of other HLA alleles and polymorphisms ... The invention relies on using a solid phase having a flat surface arrayed with oligonucleotides designed to specifically ...http://www.google.fr/patents/US7615350
Patente US6428752 - Cleaning deposit devices that form microarrays and the like - Google Patentes
A mobile-fluid storage device resupplies the deposit device along the array, e.g. in the immediate vicinity of the deposit ... for microscopic analysis, a deposit device, e.g. a pin, cooperating with a fluid source defines a precisely sized drop of fluid ... By repeated action, minute drops of fluid can be deposited precisely in a dense array, preferably under computer control. ... For depositing fluid dots in an array, e.g., ... cells and tissue fragments; lipids, lipoproteins, and the like ...http://www.google.es/patents/US6428752?dq=flatulence
Immunoliposome-nucleic acid amplification (ILNAA) assay - O'LEARY TIMOTHY J.
The first uses atomic force microscopy to perform micro-miniaturized immunoassays on compositionally patterned antibody arrays ... Introduction of SV40-DNA into tissue culture cells by the use of DNA-loaded reconstituted Sendai virus envelopes" Exp. Cell Res ... Mason, J. T. and Huang, C. (1978) "Hydrodynamic analysis of egg phosphatidylcholine vesicles" Ann. NY Acad., Sci. 308, 29-49. ... No method is known for modifying the antibody-DNA complex (or antibody streptavidin-biotin complex) directly to inhibit non- ...http://www.freepatentsonline.com/y2005/0158372.html
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