Data analysis. The goal of the microarray analysis was to identify specific antibodies that can be used to differentiate ... For immunohistochemistry (IHC) experiments, independent tissue samples were used. All tissue samples (n = 20) had both DCIS and ... Two representative arrays showing the regular pattern of spotted control proteins (left panel, subarray incubated with buffer ... 1A). As expected, all controls (Alexa Fluor 647-conjugated antibody, anti-human IgG antibody and human IgG) were highly ...http://clincancerres.aacrjournals.org/content/18/7/1992
Antibody microarray - Wikipedia
... of antibody arrays is comparable to that of ELISA and antibody arrays are regularly used for profiling experiments on tissue ... "Protein expression profiling by antibody array analysis with use of dried blood spot samples on filter paper". Journal of ... Currently the largest commercially available sandwich-based antibody array detects 1000 different proteins. Antibody arrays are ... An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a ...https://en.wikipedia.org/wiki/Antibody_microarray
Frozen tissue array - Wikipedia
Consequently frozen tissue array should be the best tool for high throughput analysis on this purpose. Frozen tissue cores with ... especially analyze different protein levels with antibodies by immunohistochemistry. Various paraffin tissue arrays are now ... Frozen tissue array consists of fresh frozen tissues in which up to 50 separate tissue cores are assembled in array fashion to ... Tissue microarrays from frozen tissues-OCT technique. Methods Mol Biol. 2010, 664:73-80. "Frozen Tissue Array". BioChain ...https://en.wikipedia.org/wiki/Frozen_tissue_array
Tissue microarray - Wikipedia
Tissue microarrays are particularly useful in analysis of cancer samples. One variation is a Frozen tissue array. The use of ... Battifora H: The multitumor (sausage) tissue block: novel method for immunohistochemical antibody testing. Lab Invest 1986, 55: ... Tissue microarrays (also TMAs) consist of paraffin blocks in which up to 1000 separate tissue cores are assembled in array ... These tissue cores are then inserted in a recipient paraffin block in a precisely spaced, array pattern. Sections from this ...https://en.wikipedia.org/wiki/Tissue_microarray
Ciphergen ProteinChip array. On each spot of PS1 series ProteinChip array, 1 μg of purified 6E10 monoclonal antibody (Signet ... Immunoblot analysis and ELISA. Mouse tissues were dissected and homogenized in T-PER buffer (Pierce, Rockford, IL) in the ... As control, the same membrane was stripped and blotted with β3-tubulin antibodies. BACE1+/+ tissue from the following: 1, ... For BACE1 immunostaining, an IgG purified anti-BACE1 fusion protein antibody was applied (1 μg/μl) as primary antibody in Tris- ...http://www.jneurosci.org/content/25/50/11693
After analysis, we first excluded all probe sets that showed no change in expression between the two arrays and those that were ... Tissue was homogenized on ice using a ground glass homogenizer. The routine Trizol protocol was modified to deal with tissue of ... C and detected with Texas Red-conjugated goat anti-rabbit antibody (Ab) (4 μg/ml; Invitrogen, Eugene, OR). In some experiments ... Scanning and data analysis.. MOE430A arrays were scanned on the GeneChip Scanner 3000 and analyzed using Affymetrix GeneChip ...http://www.jneurosci.org/content/26/48/12576
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Cell and tissue culturing in normoxia and hypoxia. - Expression and secretion analysis. - Other methods of molecular and cell ... Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Antibodies. Antigens and Kits. Cell Separation Media & Uni-Sep Tubes. Chick Embryo Extracts. Complements and Mouse Reagents. ... Multiplex Suspension Arrays Bio-Plex System and Arrays. / ?? , #13308 /. Bio-Rad Laboratories Headquarters 1000 Alfred Nobel ...http://molbiol.ru/forums/index.php?act=catalog&show=sel&can=tech
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Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Mix-n-Stain Antibody Labeling Kits. : A revolutionary protein labeling technology that allows anyone to optimally label an ... and Tissue MicroArrays. Markers & Electrophoresis Reagents. miRNA (microRNA) Studies. Northern Analysis. Nuclease Control. ... DNA-binding Dye: This is the only dye that works perfectly for both qPCR & sensitive melt curve analysis. CF dyes. : A series ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&bb_products=60
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Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Antibodies. Antigens and Kits. Cell Separation Media & Uni-Sep Tubes. Chick Embryo Extracts. Complements and Mouse Reagents. ... and Tissue MicroArrays. Markers & Electrophoresis Reagents. miRNA (microRNA) Studies. Northern Analysis. Nuclease Control. ... Gene Expression Array. ABI PRISM Primers/ Probes. Biochromatography. Cellular Detection Systems. DNA Sequencing/ Genotyping. ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&show=t_select&s_title=a
BACE1, a Major Determinant of Selective Vulnerability of the Brain to Amyloid-β Amyloidogenesis, is Essential for Cognitive,...
Ciphergen ProteinChip array. On each spot of PS1 series ProteinChip array, 1 μg of purified 6E10 monoclonal antibody (Signet ... Immunoblot analysis and ELISA. Mouse tissues were dissected and homogenized in T-PER buffer (Pierce, Rockford, IL) in the ... For BACE1 immunostaining, an IgG purified anti-BACE1 fusion protein antibody was applied (1 μg/μl) as primary antibody in Tris- ... ProteinChip Array, and Aβ ELISA analyses. Equal amounts of lysates were subject to SDS-PAGE (Tris- glycine mini gel; Invitrogen ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2564291/?lang=en-ca
Alternative splicing - Wikipedia
... arrays from ExonHit or Jivan) have been used. The array is then probed with labeled cDNA from tissues of interest. The probe ... In microarray analysis, arrays of DNA fragments representing individual exons (e.g. Affymetrix exon microarray) or exon/exon ... A trans-acting splicing regulatory protein of interest is then precipitated using specific antibodies. When the RNA attached to ... Most EST libraries come from a very limited number of tissues, so tissue-specific splice variants are likely to be missed in ...https://en.wikipedia.org/wiki/Alternative_splicing
Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity | Diabetes
Analysis of expression arrays.. Differences in WAT expression of mRNAs/miRNAs between obese/nonobese subjects and during ... Primary antibodies detecting MYC-associated factor X (MAX) were unspecific and, therefore, could not be used. ... A.K., M.C., S.L.-C., A.E., J.L., P.H., and J.-F.T. collected tissue and performed the functional analyses. P.J.B., M.P., K.D.-W ... M.R. collected tissue, performed the functional analyses, and wrote the manuscript. P.A. designed the study and wrote the ...http://diabetes.diabetesjournals.org/content/61/8/1986.full
Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays -...
While direct analysis of tumor tissue is frequently difficult or impossible (such as in instances of occult, unrecognized ... The plates are then incubated at room temperature for 30 minutes after adding 50 .mu.L per well of anti-FITC antibody-HRPO ... affords a wide array of clinical utility, including the description, characterization, or classification of the tumor, whether ... biochemical analysis, and immunologic analysis. In the preferred embodiment, amplified DNA is isolated--for example by excising ...http://www.patentgenius.com/patent/6939675.html
Frontiers | Effect of P2X4 and P2X7 receptor antagonism on the pressure diuresis relationship in rats | Physiology
Statistical analysis was performed by Mann-Whitney U-test (for Western analysis) or by unpaired t-test (physiological data). ... The P2X4 antibody detected a band of ~60 kDa; the P2X7 antibody detect a band at ~75 kDa. The autoradiogram was scanned and ... The 16 CEL intensity files were imported into Bioconductor and arrays normalized by the Robust Multi-array Average (RMA) method ... They were not tissue specific and assumed no mutual exclusivity with inflammatory, or other disease, processes. The Endeavour ...https://www.frontiersin.org/articles/10.3389/fphys.2013.00305/full
Nuclear pore complex proteins mark the implantation window in human endometrium | Journal of Cell Science
Z.N.B. performed tissue array analyses. A.J.P. and S.M.K. collected site 1 biopsies. N.S. coordinated biopsy collection and, ... Antibodies. Mouse IgGs (Covance Research Products, Princeton, NJ) of mAb414 (Davis and Blobel, 1986) were used at 2 μg/ml for ... 4B). Moreover, analysis of tissue arrays containing six paraffin sections each of human esophagus, stomach, liver, colon, ... Tissue arrays used were 59 endometrial carcinomas (adenocarcinomas grade I-III) (Cybrdi, Frederick, MD), normal tissue of ...http://jcs.biologists.org/content/121/12/2037
A randomised controlled phase II trial of pre-operative celecoxib treatment reveals anti-tumour transcriptional response in...
Images of the arrays were quantified with GCOS software (Affymetrix). The chip description file (CDF) used for the analysis was ... Chalkley H: Method for the quantitative morphologic analysis of tissues. J Natl Cancer Inst. 1943, 4: 47-53.Google Scholar ... Cell proliferation was assessed using the MIB1 mouse monoclonal antibody to Ki-67 . Measurement of apoptosis was performed ... KK provided surgical support in tissue acquisition and critically revised the manuscript. TA performed microarray analyses and ...https://rd.springer.com/article/10.1186/bcr3409
Patent US7092557 - Method and device for selectively targeting cells within a three-dimensional ... - Google Patents
For example, four populations of primary prostate cells are purified for mRNA analysis by binding antibodies against specific ... and DNA array comparison of normal and cancerous tissues have been used to investigate the distinct patterns of gene expression ... For example, one antibody labeled with phycoerythrin (PE) and another antibody labeled with Texas Red could be used to identify ... Cell Analysis Systems, Inc.. Method and apparatus for automated analysis of biological specimens. ...http://www.google.com/patents/US7092557?dq=patent:+7360079
Patent US6642018 - Method for inducing a response in one or more targeted cells - Google Patents
For example, four populations of primary prostate cells are purified for mRNA analysis by binding antibodies against specific ... and DNA array comparison of normal and cancerous tissues have been used to investigate the distinct patterns of gene expression ... For example, one antibody labeled with phycoerythrin (PE) and another antibody labeled with Texas Red could be used to identify ... Tissue Engineering. In another application, the present apparatus is used to remove contaminating cells in inocula for tissue ...http://www.google.com/patents/US6642018?dq=6175559
Neural transcriptome reveals molecular mechanisms for temporal control of vocalization across multiple timescales | BMC...
Transcriptome analyses highlighted a suite of molecular mechanisms that regulate vocalization over behaviorally relevant ... Supporting evidence from previous studies in midshipman strongly validate the value of transcriptomic analyses for linking ... We used RNA sequencing-based transcriptome analyses to compare patterns of gene expression in VMN to the surrounding hindbrain ... H tissue comparisons. Tissue-based normalization. TMM normalization (Figure 4), used by default Trinity analysis pipeline [113 ...https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-1577-2
Aberrant STYK1 expression in ovarian cancer tissues and cell lines
An ovarian tissue array (Pantomics, Inc.) was stained with STYK1 antibody. (A) Each tissue section was assigned a score of 0 ... Statistical analysis. Statistical analyses were performed using the one-way ANOVA test in GraphPad Prism (San Diego, CA). The ... Tissue panel and immunohistochemistry. Formalin-fixed arrays of normal, benign, and malignant ovarian tissues were obtained ... Antibodies. STYK1 and GPR30 antibodies were purchased from AbCam (Cambridge, MA). α-Tubulin antibody was purchased from ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2773766/
RKIP phosphorylation and STAT3 activation is inhibited by oxaliplatin and camptothecin and are associated with poor prognosis...
Samples were divided and either saved for Western blot analysis (input) or incubated with an antibody to gp130 for 6 h. Protein ... All tissue samples were formalin fixed and paraffin embedded. The corresponding H&E slides were reviewed for confirmation of ... Three to six cores of tumor were arrayed per specimen. In addition, a core of normal adjacent colonic mucosa was also sampled ... Statistical analysis. Chi-square analysis was used to evaluate the association between STAT3 expression and tumor grade and ...https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-463
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Cell and tissue culturing in normoxia and hypoxia. - Expression and secretion analysis. - Other methods of molecular and cell ... Array Analysis. Automation/High Throughput. Buffers and Chemicals. Custom Products & Services. Enzymes. In Situ Hybridization ... Antibody production in a wide range of animal models as well as Peptide-, Antibody- and ProteinArrays are also amongst its ... Multiplex Suspension Arrays Bio-Plex System and Arrays. / ?? , #13308 /. Bio-Rad Laboratories Headquarters 1000 Alfred Nobel ...http://molbiol.ru/forums/index.php?act=catalog&can=tech&bb_location=12
Patent US6043053 - Wnt-3 polynucleotides - Google Patents
"Antibodies" as used herein includes polyclonal and monoclonal antibodies, chimeric, single chain, and humanized antibodies, as ... Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and ... Array technology methods are well known and have general applicability and can be used to address a variety of questions in ... Nucleic acids for diagnosis may be obtained from a subject's cells, such as from blood, urine, saliva, tissue biopsy or autopsy ...http://www.google.com/patents/US6043053
US20050069900A1 - Analyte sample detection - Google Patents
The methods may be performed in multiplex form to permit simultaneous analysis of a plurality of targets. Kits for performing ... Method for automated processing of digital images of tissue micro-arrays (TMA) ... The filter is then contacted with a second antibody linked to a peroxidase enzyme to form an antibody:antigen:antibody-enzyme ... Flow cytometric analysis of marine bacteria with Hoechst 33342. Shapiro 2000. Microbial analysis at the single-cell level: ...https://patents.google.com/patent/US20050069900A1/en
US7544471B2 - Preparing RNA from a wax-embedded tissue specimen - Google Patents
Methods of performing an array analysis of a labeled RNA sample are also described. ... a wax-embedded tissue specimen is digested and the resulting digested tissue specimen is purified to provide a purified RNA ... Representative capture agents include polypeptides and polynucleotides, for example antibodies, peptides, or fragments of ... A method of performing an array analysis comprising: labeling a plurality of miRNAs from a wax-embedded tissue specimen using a ...https://patents.google.com/patent/US7544471?oq=7%2C346%2C545