Retroviral Replicating Vector That Delivers Cytosine Deaminase to Cancer Cells Active in Recurrent Glioblastoma - The ASCO Post
A phase I study by Cloughesy et al published in Science Translational Medicine investigating the effectiveness of vocimagene amiretrorepvec (Toca 511), an experimental nonlytic, retroviral replicating vector that delivers cytosine deaminase to cancer cells, and an investigational extended-release version of 5-fluorocytosine (Toca FC) in patients with recurrent glioblastoma has found that the treatment increased overall survival to 13.6 months vs 7.1 months for an external control.. According to the investigators, standard treatments for recurrent glioblastoma are associated with an overall survival of 7.1 to 9.8 months. For some study participants, survival was extended to more than 2 years. The therapy was associated with few side effects as well as a molecular signature that may correlate with treatment-related survival.. Based on these study results, a phase II/III international clinical trial (Toca5) in recurrent glioblastoma and anaplastic astrocytoma is underway.. ...http://ascopost.com/News/41605
The human cytidine deaminase Apobec3F (h-A3F) a protein linked to the - Aurora Kinases as Druggable Targets in Cancer Therapy
The human cytidine deaminase Apobec3F (h-A3F) a protein linked to the previously recognized antiviral factor Apobec3G (h-A3G) has antiviral activity against individual immunodeficiency virus type 1 (HIV-1) that's suppressed with the viral protein Vif. E3 ubiquitin ligase. Disturbance with Cul5-E3 ligase function by depletion of Cul5 through RNA disturbance or overexpression of Cul5 mutants obstructed the power of HIV-1 Vif to suppress h-A3F. A BC-box mutant of HIV-1 Vif that didn't recruit Cul5-E3 ligase but was still in a position to connect to h-A3F didn't suppress h-A3F. Oddly enough disturbance with Cul5-E3 ligase function or overexpression of h-A3F or h-A3G also elevated the balance of HIV-1 Vif recommending that just like the substrate substances h-A3F and h-A3G the substrate receptor proteins Vif is certainly itself also governed by Cul5-E3 ligase. Our outcomes indicate that Cul5-E3 ligase is apparently a common pathway hijacked by HIV-1 Vif to beat both h-A3F and h-A3G. Developing ...http://www.exposed-skin-care.net/2017/02/28/the-human-cytidine-deaminase-apobec3f-h-a3f-a-protein-linked-to-the/
APOBEC proteins and intrinsic resistance to HIV-1 infection | Philosophical Transactions of the Royal Society B: Biological...
As noted in §5, A3G is but one out of the 11 members of the human APOBEC protein family. Accordingly, multiple groups have examined the anti-HIV-1 properties of all the APOBEC proteins, and the results have been catalogued in recent review papers (Holmes et al. 2007b; Chiu & Greene 2008). The most significant other human protein with respect to HIV-1 is APOBEC3F (A3F): it is a strong inhibitor of vif-deficient HIV-1, though not as potent as A3G (Bishop et al. 2004a; Liddament et al. 2004; Wiegand et al. 2004; Zheng et al. 2004; Zennou & Bieniasz 2006), and is the family member that is most closely related to A3G in that they share approximately 50 per cent sequence identity. A3F is also efficiently inhibited by HIV-1 Vif via the recruitment of the cullin5-elongin B/C-Rbx ubiquitin ligase and ensuing proteasomal degradation (Liu et al. 2005). These shared features of A3G and A3F seemingly accord with the view that these are the most significant APOBEC enzymes in terms of natural HIV-1 infection ...http://rstb.royalsocietypublishing.org/content/364/1517/675
Genetic editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G. - pdf...
Genetic editing of HBV DNA by monodomain human APOBEC3 cytidine deaminases and the recombinant nature of APOBEC3G.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.http://libros.duhnnae.com/2017/jul7/150116701897-Genetic-editing-of-HBV-DNA-by-monodomain-human-APOBEC3-cytidine-deaminases-and-the-recombinant-nature-of-APOBEC3G.php
Toca 511 and Toca FC - Wikipedia
Toca 511 and Toca FC is a combination drug involving a gene therapy agent and a prodrug. It is a candidate drug to treat brain cancers. Toca 511 (vocimagene amiretrorepvec) is a gene therapy agent, wherein the payload is a gene encoding cytosine deaminase (CD) in a replicating, non-lytic retroviral vector. Toca FC is an extended-release formulation of the antifungal drug, 5-fluorocytosine, which is a prodrug of 5-fluorouracil, a known cancer drug. 5-fluorouracil does not cross the blood-brain barrier well, but 5-fluorocytosine does. The combination drug was designed to be used after a brain tumor is removed surgically; Toca 511 is intended to be injected into the tissues lining the hole where the tumor was (this region is called the margin), where the virus replicates only in cells that are dividing - in other words, cancer cells left over in the margin and immune cells that are present. 5-fluorocytosine is then administered to the person, ...https://en.wikipedia.org/wiki/Toca_511_and_Toca_FC
The crystal structure of APOBEC-2 and implications for APOBEC enzymes :: University of Southern California Dissertations and...
APOBEC-2 (Apo2) belongs to the Apolioprotein B (APOB) mRNA-editing enzyme catalytic polypeptide (APOBEC) family of cytidine deaminases that modify genes by deaminating cytosines in mRNA coding sequences and cytidines in ssDNA (Franca et al., 2006). APOBEC deaminases have remarkably diverse functions comprising antibody maturation, inactivation of viral genomes including Human Immunodeficiency virus-1 (HIV-1) and Hepatitis B virus (HBV), inhibition of retrotransposition, and RNA editing. The repertoire of APOBECs and their respective activities are continuously expanding to include new functions and sub-classes of the family.; The APOBEC family is composed of APOBEC-1 (Apo1), Apo2, AID, and APOBEC-3 (3A, 3B, 3C, 3DE, 3F, 3G, and3H) and APOBEC-4 (Conticello et al., 2004; Sawyer et al., 2004). Previous comparative modeling of APOBECs has been performed using the structures of free nucleotide cytidine deaminases (fntCDA) which deaminate free cytidine/cytosine bases which share ...http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/39667/rec/20
Biology | Free Full-Text | A Comparison of Two Single-Stranded DNA Binding Models by Mutational Analysis of APOBEC3G
APOBEC3G is the best known of several DNA cytosine deaminases that function to inhibit the replication of parasitic genetic elements including the lentivirus HIV. Several high-resolution structures of the APOBEC3G catalytic domain have been generated, but none reveal how this enzyme binds to substrate single-stranded DNA. Here, we constructed a panel of APOBEC3G amino acid substitution mutants and performed a series of biochemical, genetic, and structural assays to distinguish betweenhttp://mdpi.com/2079-7737/1/2/260/xml
Neural stem cell-based dual suicide gene delivery for metastatic brain tumors.
In our previous works, we demonstrated that human neural stem cells (NSCs) transduced with the cytosine deaminase (CD) gene showed remarkable 'bystander killer effect' on glioma and medulloblastoma cells after administration of the prodrug 5-fluorocyhttp://www.biomedsearch.com/nih/Neural-stem-cell-based-dual/23018623.html
UAB - School of Medicine - Program in Immunology - G. Yancey Gillespie, PhD
The main thrust of my research is to develop and test specific therapies for treatment of malignant brain tumors in adults and children. One current focus is construction of replication conditional herpes simplex viruses that are both oncolytic for glioma cells and express foreign therapeutic genes. Gene transfer includes both pro-drug converting enzymes and cytokines under different promoter systems. Pro-drug enzyme systems currently being studied are cytosine deaminase (CD) alone or as a fusion protein with uracil phosphoribosyl transferase (UPRT) and purine nucleoside phosphorylase (PNP). A second focus involves studies cytokines expressed from replication competent HSV that include TNFa, IL-2, IL-4, IL-5, IL-10, IL12, IL-16. These systems are validated by in vitro assays first before being advanced to safety and efficacy assessment in a variety of murine models of intracranial malignant gliomas. These models include transplantable intracranial gliomas of human origin (in ...http://www.uab.edu/medicine/immunology/faculty?id=100:gillespie&catid=39:faculty
A Pilot Feasibility Study of Oral 5-Fluorocytosine and Genetically-Modified Neural Stem Cells Expressing E.Coli Cytosine...
PRIMARY OBJECTIVES:. I. To determine the safety and feasibility of intracerebral administration of NSCs in combination with oral 5-FC in patients with recurrent high-grade gliomas.. SECONDARY OBJECTIVES:. I. To characterize the relationship between intracerebral and systemic concentrations of 5-FC and 5-FU with increasing NSC dose level.. II. To non-invasively assess the presence of 5-FU in the brain with the use of fluorine (19F)-magnetic resonance spectroscopy (MRS)(no longer in effect as of 5/1/2012).. III. To assess for the possible development of immunogenicity against the NSCs.. IV. To assess the intracerebral distribution of NSCs using iron-labeling as a cellular tracker.. V. To gather preliminary imaging data regarding perfusion permeability parameters and imaging characteristics as shown on magnetic resonance imaging (MRI) studies due to the presence of NSCs in the brain.. VI. To determine, at time of autopsy, the fate of the NSCs.. OUTLINE:. This is a dose-escalation study.. After ...https://clinicaltrials.gov/ct2/show/record/NCT01172964
Recombinant Human APOBEC1 protein (ab116768) | Abcam
Buy our Recombinant Human APOBEC1 protein. Ab116768 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam provides…http://www.abcam.com/recombinant-human-apobec1-protein-ab116768.html
Structural Characterization of APOBECS's Atomic interactions - Celia Schiffer
The APOBECS's (A3G, ASF and possibly A3H) are cellular DNA cytosine deaminases that are key innate anti-viral agents, particularly in response to HIV-1. Althoug...http://grantome.com/grant/NIH/P01-GM091743-02-6213
APOBEC3A deaminates transiently exposed single-strand DNA during LINE-1 retrotransposition | eLife
Combining in vitro and in vivo assays demonstrates that the cytidine deaminase APOBEC3A can inhibit LINE-1 retrotransposition by deaminating transiently exposed single-strand DNA that arises during the process of LINE-1 integration.https://elifesciences.org/articles/02008
APOBEC3G polyclonal antibody - (PAB30317) - Products - Abnova
Rabbit polyclonal antibody raised against partial recombinant human APOBEC3G. Recombinant protein corresponding to human APOBEC3G. (PAB30317) - Products - Abnovahttp://www.abnova.com/products/products_detail.asp?catalog_id=PAB30317
Pik3cd - Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform - Mus musculus (Mouse) - Pik3cd gene &...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...http://www.uniprot.org/uniprot/Q3UDT3
APOBEC3A is the primary mutagenic cytidine deaminase in huma
Chan et al. 'An APOBEC3A hypermutation signature is distinguishable from the signature of background mutagenesis by APOBEC3B in human cancers' Nature Genethttps://www.labroots.com/videos/1446/apobec3a-primary-mutagenic-cytidine-deaminase-human-cancers
CD3 epsilon抗体[C363.29B (YCD3-1)] |Abcam中国|Anti-CD3 epsilon抗体[C363.29B (YCD3-1)]
CD3 epsilon大鼠单克隆抗体[C363.29B (YCD3-1)](ab25469)可与小鼠样本反应并经IP, Flow Cyt实验严格验证，被4篇文献引用。所有产品均提供质保服务，中国75%以上现货。http://www.abcam.cn/cd3-epsilon-antibody-c36329b-ycd3-1-ab25469.html
5-FC (5-Fluorocytosine) Assay
Updated: 07/24/2014. Note: The information contained in this handbook is for use by personnel of University of Iowa Health Care. No other use is implied or intended.. Vacutainer® and/or Microtainer® are registered trademarks of Becton, Dickinson & Company.. ...https://www.healthcare.uiowa.edu/path_handbook/handbook/test2.html
anti-ADAD2 antibody, C-term | GeneTex
ADAD2 antibody, C-term (adenosine deaminase domain containing 2) for WB. Anti-ADAD2 pAb (GTX47217) is tested in Human samples. 100% Ab-Assurance.http://www.genetex.com/ADAD2-antibody-C-term-GTX47217.html
Browse our APOBEC1 ELISA Kits
Order APOBEC1 ELISA Kits for many Reactivities. and more. Compare APOBEC1 ELISA Kits and find the right product on antibodies-online.com.http://www.antibodies-online.com/abstract/Apolipoprotein+B+MRNA+Editing+Enzyme%2C+Catalytic+Polypeptide+1+
Double Suicide - Everything2.com
Chikamatsu Monzaemon|Chikamatsu Monzaemon's play 'The Lovers Suicides at Amijima' was made into a film by Masahiro Shinoda in 1969. Its name r...https://everything2.com/title/Double+Suicide
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yCD A z Brian Eno u C C [ m / Nerve Net ̂ ??? Ȃ KDDI/KDDI R } [ X t H [ h ^ c l b g V b s O E ʔ̃T C g uWowma! v B E I ɂȂ?? ̃T C gWowma! i E } j B l C ̃A C e W I2,000 i ȏ?? L x ȕi 낦 ???? i Z [ i ??? f ڒ I | C g N [ | g ?? OK I Ȃ ̗~ ??ƌ ??https://wowma.jp/item/113648159
cytosine | chemical compound | Britannica.com
cytosine: A nitrogenous base derived from pyrimidine that occurs in nucleic acids, the heredity-controlling components of all living cells, and in some coenzymes, substances that act in...https://www.britannica.com/science/cytosine
Molecule Of Cytosine, Key Component In The Formation Of Stable.. Stock Photo, Picture And Royalty Free Image. Image 16678181.
Picture of Molecule of cytosine, key component in the formation of stable.. stock photo, images and stock photography.. Image 16678181.https://www.123rf.com/photo_16678181_molecule-of-cytosine-key-component-in-the-formation-of-stable-dna-and-rna-molecules-3d-model.html