Progress in the delivery and transgene detection methods, together with optimization approaches for improved on-target ... bringing new opportunities for the development of transgene-free improved varieties of economically important crops. This ... The use of the system demonstrated the efficacy of the suicide transgenes BARNASE and CMS2 in producing transgene-free rice ... The approach is valid; however rapid screening of transgene and transgene-free mutant plants is hindered by the lack of ...

Transgene-specific immune response. Abs, T-cells. Abs, T-cells. Abs, T-cells. ... although the transgene must then compete with equally-amplified vector antigens for immunogenicity. Viral vectors have been ...

... entry and improved transgene expression in human cells. Moreover, addition of a targeting ligand into the nanocomplex system, ... engineered to carry a mammalian transgene cassette, resulted in increased cellular attachment, ... the size of the transgene and whether the resulting output requires transient or stable transgene expression. To achieve ... engineered to carry a mammalian transgene cassette, resulted in increased cellular attachment, entry and improved transgene ...

Viswanathan, M.; Guarente, L. Regulation of Caenorhabditis elegans lifespan by sir-2.1 transgenes. Nature 2011, 477, E1-E2. [ ...

At least half of the 2n green plants were transgenic, and their progeny were shown to carry the transgene. The process of ... dihaploidization did not affect the expression of the transgene. Embryo cultures were used to reduce the time to seed of the ...

Chen, H.; Ke, Q.; Kluz, T.; Yan, Y.; Costa, M. Nickel ions increase histone H3 lysine 9 dimethylation and induce transgene ...

... was performed to amplify the transgene. The sequences of the transgenes were confirmed by Sanger Sequencing (Genewiz, South ...

Co-expression of multiple transgenes in mouse CNS: A comparison of strategies. Biomol. Eng. 2001, 17, 157-165. [Google Scholar ...

It would be interesting to look at strains with spatially and/or chronologically controlled transgene expression. For example, ...

The transfection was repeated several times for the maintenance of the transgene, which could be nicely proven (Figure 2). The ... The SV40 transgene is replicated extrachromosomally during the synthesis phase of cell cycle. The stimulated DNA synthesis, ... In future studies a retroviral vector could be used for permanent SV40 transformation which allows integration of the transgene ...

Hubner, A.; Keil, G.M.; Kabuuka, T.; Mettenleiter, T.C.; Fuchs, W. Efficient transgene insertion in a pseudorabies virus vector ...

Transgene-Free Ex Utero Derivation of a Human Post-Implantation Embryo Model Solely from Genetically Unmodified Naïve PSCs. ...

Spontaneous atopic dermatitis in mice expressing an inducible thymic stromal lymphopoietin transgene specifically in the skin. ...

Transgenes can be expressed using self-replicating plasmids (episomal), which requires active use of one or more selection ... Strong, inducible promoters are ideal to control transgene expression, although promoters of varying strengths can help to fine ... The latter approach has the advantage that the transgene will be genetically inherited without active selection. While ... and expressing no transgene (empty vector) or sfGFP under control of the methanol-inducible pAOX1, the constitutive pTEF, or ...

A human IL10 BAC transgene reveals tissue-specific control of IL-10 expression and alters disease outcome. Proc. Natl. Acad. ...

Porcine adiponectin receptor 1 transgene resists high-fat/sucrose diet-induced weight gain, hepatosteatosis and insulin ...

First, we developed a Tol2 transposon vector carrying the Cas13a-msGFP-NLS (pT-Cas13a) transgene, followed by a stable ... Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after ... insertion of the Cas13a transgene into the genome of DF1 cells to generate stable DF1-Cas13a cells. To assess the Cas13a- ...

The AtHFR1 transgene represses transcription of TaFT1, TaCO1, and TaCO2, delaying development of the shoot apex and heading in ... Furthermore, the AtHFR1 transgene in wheat inhibits transcript levels of PHYTOCHROME-INTERACTING FACTOR 3-LIKEs (TaPIL13, ... indicating that the AtHFR1 transgene can activate the light signal transduction pathway in wheat. AtHFR1 transgenic seedlings ...

In Vivo and in Vitro Effects of Ultrasound or/and Microbubbles on Recombinant Adeno-Associated Virus-Mediated Transgene ...

PActin-GAL4 vector (pAcGal4, Addgene #24344) was used in S2 cells co-transfection experiments to drive transgene expression ...

To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through ... To sustain gene delivery and elongate transgene expression, plasmid DNA and cationic nonviral vectors can be deposited through ...

The first type directly produce PrPSc derived from exogenous mutant PrP transgenes so that the mice suffer from prion disease ... since all forms were shown to be expressed on the cell surface and the levels of transgene expression were comparable among the ...

Pto was further found to specifically phosphorylate Pto-interacting 1 (Pti1), and the expression of the Pti1 transgene resulted ...

... transgenes. Using a chronic low-dose exposure PQ protocol (0.75 mM) during the entire length of the experiment (PQ treated), ...

Moreover, the expression of an IKKβCA transgene, that leads to the constitutive activation of p50 and RelA (but not c-Rel, ... mice expressing only one copy of the transgene, do not develop autoimmunity but mount strong anti-tumor immune responses, ...

Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges ...

Subsequent PCR-mediated analyses proved integration of the transgene at a different genomic location in each transformant and ... In addition, helper-dependent (high-capacity) adenoviral vectors allow sustained transgene expression in this organism. Taking ...

Recombinant viruses can be engineered to express foreign transgenes and have a broad tropism allowing gene expression in a wide ... Recombinant viruses can be engineered to express foreign transgenes and have a broad tropism allowing gene expression in a wide ...

... we showed that AAV vectors carrying donor templates can facilitate the CRISPR-mediated precise integration of transgenes within ...

Selenium uptake studies corroborated the transfection results, where significant transgene expression was evident for the ...