Research - The Sun Laboratory
... we found both loss of function of mutant FUS/TLS by decreased interaction with U1 snRNP (recognize 5' splice site as a core ... This could sequester certain RNA-binding proteins and lead to their loss of function. An alternative but not mutually exclusive ... A third hypothesis is the expansions cause gene silencing and lead to haploid insufficiency of C9orf72 protein. Till today, ... These steps are regulated by RNA-binding proteins (RBPs) and dysfunctions of RNA metabolism causes various human diseases. In ...http://labs.pathology.jhu.edu/sun/research/
UC Irvine - Faculty Profile System
2006) PP1/PP2A phosphatases are required for the second step of pre-mRNA splicing and target specific snRNP proteins. Mol Cell ... this complex consists of more than 85 proteins, including the core 3' processing factors and many peripheral factors that may ... 2015) The end of the message: multiple protein-RNA interactions define the mRNA polyadenylation site. Genes & Dev. 29: 889-897. ... We have mapped the RNA interactions for some of the core mRNA 3' processing factors (Yao et al., PNAS 2012; Yao et al., RNA ...https://www.faculty.uci.edu/profile.cfm?faculty_id=5699
LSM10 - Wikipedia
"A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre- ... Pillai RS, Grimmler M, Meister G, Will CL, Lührmann R, Fischer U, Schümperli D (Sep 2003). "Unique Sm core structure of U7 ... U7 snRNA-associated Sm-like protein LSm10 is a protein that in humans is encoded by the LSM10 gene. LSM10 has been shown to ... "Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein". The EMBO Journal. 20 (19 ...https://en.wikipedia.org/wiki/LSM10
Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes |...
A role for the yeast La protein in U6 snRNP assembly: evidence that the La protein is a molecular chaperone for RNA polymerase ... a novel U6 snRNA-associated protein with significant similarity to core proteins of small nuclear ribonucleoproteins. EMBO J. ... J. L. (1995). Structurally related but functionally distinct yeast SmD core small nuclear ribonucleoprotein particle proteins. ... Séraphin, B. (1995). Sm and Sm-like proteins belong to a large family: identification of proteins of the U6 as well as the U1, ...http://jcs.biologists.org/content/120/24/4310.full
Repositório da Universidade de Lisboa: Transcription dynamics prevents RNA-mediated genomic instability through SRPK2-dependent...
... core components of the U5 1 2 snRNP, does not drive genomic instability. Altogether, we found that phosphorylation of DDX23 by ... Phosphorylation/de phosphorylation cycles regulate the function of SR-proteins and the timely binding to cis regulatory ... DDX23 is part of the spliceosomal U5 small nuclear ribonucleoprotein complex (U5 snRNP). We found that the role of DDX23 in ... Embora a proteína DDX23 faça parte do complexo ribonucleoproteíco U5 snRNP do spliceossoma, demonstrámos que o seu papel na ...http://repositorio.ul.pt/handle/10451/34144?locale=pt_PT
U6 spliceosomal RNA - Wikipedia
Shannon KW, Guthrie C (May 1991). "Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs ... Butcher SE, Brow DA (June 2005). "Towards understanding the catalytic core structure of the spliceosome". Biochem. Soc. Trans. ... U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclear ribonucleoprotein), an RNA-protein ... The association of U6 snRNP with U2 snRNP via base-pairing forms the U6-U2 complex, a structure that comprises the active site ...https://en.wikipedia.org/wiki/U6_spliceosomal_RNA
U7 small nuclear RNA - Wikipedia
"A novel zinc finger protein is associated with U7 snRNP and interacts with the stem-loop binding protein in the histone pre- ... Schümperli D, Pillai RS (2004). "The special Sm core structure of the U7 snRNP: far-reaching significance of a small nuclear ... Azzouz TN, Gruber A, Schümperli D (2005). "U7 snRNP-specific Lsm11 protein: dual binding contacts with the 100 kDa zinc finger ... Godfrey AC, White AE, Tatomer DC, Marzluff WF, Duronio RJ (2009). "The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are ...https://en.wikipedia.org/wiki/U7_small_nuclear_RNA
Frontiers | GIGANTEA - an emerging story | Plant Science
Although it is known that both GI transcript and GI protein are clock controlled, the regulation of its abundance and functions ... Although it is known that both GI transcript and GI protein are clock controlled, the regulation of its abundance and functions ... is a plant specific nuclear protein and functions in diverse physiological processes such as flowering time regulation, light ... is a plant specific nuclear protein and functions in diverse physiological processes such as flowering time regulation, light ...https://www.frontiersin.org/articles/10.3389/fpls.2015.00008/full
HNRNPA2B1 - Wikipedia
"U1 SnRNP association with HnRNP involves an initial non-specific splice-site independent interaction of U1 SnRNP protein with ... Kumar A, Williams KR, Szer W (1986). "Purification and domain structure of core hnRNP proteins A1 and A2 and their relationship ... The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM ... and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts". Proc. Natl. Acad. Sci. U.S.A. 86 ( ...https://en.wikipedia.org/wiki/HNRNPA2B1
Gentaur Molecular :GenWay \ U6 snRNA-associated Sm-like protein LSm1 - Small nuclear ribonuclear CaSm; Cancer-associated Sm...
U6 snRNA-associated Sm-like protein LSm1 - Small nuclear ribonuclear CaSm; Cancer-associated Sm-like \ 10-288-22100F for more ... snRNP core Sm-like protein Sm-x5). [LSM3 SMX4 USS2 YLR438C-A] U6 snRNA-associated Sm-like protein LSm3 (SmX4 protein). [LSM4] ... U4/U6.U5 snRNP 27 kDa protein) (U4/U6.U5-27K) (Nucleic acid-binding protein RY-1) (U4/U6.U5 tri-snRNP-associated 27 kDa protein ... U4/U6 snRNP 61 kDa protein) (Protein 61K). [LSM8 YJR022W J1464 YJR83.16] U6 snRNA-associated Sm-like protein LSm8. [LSM3 MDS017 ...http://www.antibody-antibodies.com/product_det.php?id=1880858&supplier=search&name=U6%20snRNA_associated%20Sm_like%20protein%20LSm1%20_%20Small%20nuclear%20ribonuclear%20CaSm%3B%20Cancer_associated%20Sm_like%20
Produktübersicht anti-ZCRB1 Antikörper
It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are ... The 5'-flanking region contains an enhancer core motif and binding sites for AP-1 (zeige FOSB Antikörper), AP-2 (zeige GTF3A ... U11/U12 small nuclear ribonucleoprotein 31 kDa protein , U11/U12 snRNP 31 kDa protein , U11/U12 snRNP 31K , U11/U12-31K ... Protein Überblick Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and ...http://www.antikoerper-online.de/abstract/Zinc+Finger+CCHC-Type+and+RNA+Binding+Motif+1+
Death, autoantigen modifications, and tolerance | Arthritis Research & Therapy | Full Text
... which include Smith complex proteins found in several snRNPs, as well as U1-snRNP-specific proteins such as U1-70 kD and U1A, ... unique region protein 6 (UL6), and infected cell protein (ICP)27. All three viral RNA binding proteins have been shown to ... Several antigens including Ro, small nuclear ribonuclear protein (snRNP), and Smith complex relocalized from their normal ... First, many host proteins specifically interact with viral nucleic acid. The La protein, for example, binds to hepatitis C ...https://arthritis-research.biomedcentral.com/articles/10.1186/ar75
U2 spliceosomal RNA - Wikipedia
Kramer, A (1988). "Presplicing complex formation requires two proteins and U2 snRNP". Genes Dev. 2 (9): 1155-1167. doi:10.1101/ ... "Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution". Nature Structural & Molecular Biology. 21 (6): ... and the snRNP proteins. Previous studies indicated that the stem I in 5′ terminus of the U2 snRNA has a function in early steps ... Moreover, U2 snRNP undergoes several rearrangements along with the recognition of the branch point adenosine and formation of ...https://en.wikipedia.org/wiki/U2_spliceosomal_RNA
Frontiers | iTRAQ-Based Comparative Proteomic Analysis of Adult Schistosoma japonicum from Water Buffalo and Yellow Cattle |...
A total of 131 differentially expressed proteins (DEPs) were identified, including 46 upregulated proteins and 85 downregulated ... Further analysis indicated that these DEPs were primarily involved in protein synthesis, transcriptional regulation, protein ... A total of 131 differentially expressed proteins (DEPs) were identified, including 46 upregulated proteins and 85 downregulated ... Further analysis indicated that these DEPs were primarily involved in protein synthesis, transcriptional regulation, protein ...https://www.frontiersin.org/articles/10.3389/fmicb.2018.00099/full
STRAP - Wikipedia
"Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... Pillai RS, Grimmler M, Meister G, Will CL, Lührmann R, Fischer U, Schümperli D (2003). "Unique Sm core structure of U7 snRNPs: ... "Unrip is a component of SMN complexes active in snRNP assembly". FEBS Lett. 579 (11): 2348-54. doi:10.1016/j.febslet.2005.03. ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. doi:10.1038/ ...https://en.wikipedia.org/wiki/STRAP
Produktübersicht anti-PPP2CA Antikörper
... protein phosphatase-2A-beta , Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform , protein phosphatase 2A ... PP2B and PP1alpha (zeige PPP1CA Antikörper) cooperatively disrupt 7SK snRNP (zeige LSM2 Antikörper) to release P-TEFb (zeige ... It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, ... protein phosphatase 2a, catalytic subunit, beta isoform , protein phosphatase 2, catalytic subunit, beta isoform , protein ...http://www.antikoerper-online.de/mitotic-g1-g1/s-phases-pathway-60/ppp2ca-antibody-4620/
Alternative splicing - Wikipedia
Binding of TIA-1 protein to an intronic splicing enhancer site stabilizes binding of the U1 snRNP. The resulting 5' donor site ... In this particular case, these exon definition interactions are necessary to allow the binding of core splicing factors prior ... Most of the activator proteins that bind to ISEs and ESEs are members of the SR protein family. Such proteins contain RNA ... When the RNA attached to that protein is isolated and cloned, it reveals the target sequences for that protein. Another method ...https://en.wikipedia.org/wiki/Alternative_splicing
Nikolay G Kolev, PhD | Yale School of Public Health
The emerging role of RNA-binding proteins in the life cycle of Trypanosoma brucei. Kolev, N.G., Ullu, E. and Tschudi C. The ... In vivo assembly of functional U7 snRNP requires RNA backbone flexibility within the Sm-binding site. Kolev, N.G. and Steitz, J ... Comparative genomics reveals two novel RNAi factors in Trypanosoma brucei and provides insight into the core machinery. Barnes ... Patrick KL, Shi H, Kolev NG, Ersfeld K, Tschudi C, Ullu E: Distinct and overlapping roles for two Dicer-like proteins in the ...http://medicine.yale.edu/ysph/about/contact/nikolay_kolev-2.profile
Lab Projects | The Beggs Lab
We demonstrated that SRm300, the only SR-related protein known to be at the core of human catalytic spliceosomes, is a ... We showed that Cwc21p binds directly to two key U5 snRNP proteins, Prp8p and Snu114p. Using a combination of proteomic ... Cwc21p is a protein of unknown function that is associated with the NineTeen Complex (NTC), a group of proteins involved in ... Cus2 and the U2 snRNP join the U1 snRNP on the intron. Prp5 causes a conformational change in U2 snRNA, which interacts with ...http://beggs.bio.ed.ac.uk/content/lab-projects
Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6·U5 tri‐snRNP...
... specific proteins since no binding was observed with 10S U5 or U1 snRNP core particles (lanes 5 and 6, respectively) or with ... Protein 61K interacts with the U5 snRNP‐specific 102K protein. The 61K protein could promote tri‐snRNP formation in at least ... However, protein-protein interactions are believed to play a major part in tri‐snRNP formation. Indeed, the tri‐snRNP particle ... Interestingly, only one out of the 13 tested proteins interacted with protein 61K, namely the U5 snRNP‐specific protein 102K ( ...http://emboj.embopress.org/content/21/5/1148
Non-coding RNA - Wikipedia
The spliceosome is another RNP often also known as the snRNP or tri-snRNP. There are two different forms of the spliceosome, ... expressed in mammalian testes and somatic cells form RNA-protein complexes with Piwi proteins. These piRNA complexes (piRCs) ... B2 RNA inhibits transcription by binding to core Pol II. Through this interaction, B2 RNA assembles into preinitiation ... As with proteins, mutations or imbalances in the ncRNA repertoire within the body can cause a variety of diseases. Many ncRNAs ...https://en.wikipedia.org/wiki/Non-coding_RNA
Biophysics reveals the assembly mechanism of snRNP, a core component of the RNA splice complex ... or protein kinases are encoded by the genome? What are the largest genes and proteins? How similar are human proteins to those ... Protein Homeostasis covers the entire spectrum of protein homeostasis. 10/07/2011. Written and edited by experts in the field, ... Protein Synthesis and Translational Control. 08/29/2012. Introducing a new book on the synthesis of proteins Now available for ...https://www.cshlpress.com/events.tpl?cart=1507894909411381392
Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA‐binding protein | The EMBO Journal
At the protein level, all U1 and U4/U6 snRNP proteins are also dissociated, while at the same time numerous proteins are stably ... In yeast, these include a protein complex termed the 'nineteen complex' (NTC), that consists of eight core proteins including ... proteins, because several of them interact loosely with one or more of the NTC core proteins (Ohi and Gould, 2002). The NTC is ... Pena V, Liu S, Bujnicki JM, Lührmann R, Wahl MC (2007) Structure of a multipartite protein‐protein interaction domain in ...http://emboj.embopress.org/content/31/9/2222?ijkey=5f3c3e0a1ca8a70156816135de5e97721a9e65f4&keytype2=tf_ipsecsha
RCSB PDB - 1H64: CRYSTAL STRUCTURE OF THE SM-RELATED PROTEIN OF P. ABYSSI: THE BIOLOGICAL UNIT IS A HEPTAMER...
Crystal Structures of the Pyrococcus Abyssi Sm Core and its Complex with RNA.Common Features of RNA Binding in Archaea and ... SNRNP SM-LIKE PROTEIN 1, 2, A..., B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z1, 2, A, B, C, D, E ... CRYSTAL STRUCTURE OF THE SM-RELATED PROTEIN OF P. ABYSSI: THE BIOLOGICAL UNIT IS A HEPTAMER. *DOI: 10.2210/pdb1h64/pdb ...http://www.rcsb.org/pdb/explore/explore.do?structureId=1H64
CARRIER, METHOD AND REAGENT FOR OBTAINING SMALL RNA - Patent application
... ribosomal protein, snRNP protein, snoRNP protein, and Argonaute-family protein, and among them, Argonaute-family protein is ... surface of the core particle, it is possible to graft said polymer compound on the surface of the core particle in high density ... RNA-binding protein complex nonspecifically, the adsorbed protein may cause to inhibit the small RNA-binding protein complex, ... protein--small RNA complex is the one which comprises a complex produced by binding the small RNA-binding protein involved in ...http://www.patentsencyclopedia.com/app/20100311958