*  Cellular functions of the Rap1 GTP-binding protein: a pattern emerges | Journal of Cell Science
Small GTP-binding proteins. Introduction. The Ras superfamily of GTP-binding proteins comprises over 100 members, all ... The small GTP-binding protein Rap1 has found itself an identity in recent years. First considered as a mere antagonist of Ras ... Rap1 belongs to the Ras subgroup of small GTP-binding proteins. Whereas its early history has focused on its biochemical ... Cellular functions of the Rap1 GTP-binding protein: a pattern emerges Message Subject (Your Name) has sent you a message from ...
  http://jcs.biologists.org/content/116/3/435
*  Angiotensin II Signal Transduction Through Small GTP-Binding Proteins | Hypertension
Rap1 Activation by Ang II. Rap belongs to the Ras family of small G proteins. In pulmonary vein endothelial cells, Ang II was ... Small GTP-binding proteins (G proteins) are monomeric G proteins with a low molecular weight of 20 to 40 kDa. A small G protein ... Also, eIF4E is released from eIF4E binding protein/PHAS-I on phosphorylation of eIF4E binding protein/PHAS-I regulated through ... Angiotensin II Signal Transduction Through Small GTP-Binding Proteins. Haruhiko Ohtsu, Hiroyuki Suzuki, Hidekatsu Nakashima, ...
  http://hyper.ahajournals.org/content/48/4/534
*  Annexin A1 Counteracts Chemokine-Induced Arterial Myeloid Cell RecruitmentNovelty and Significance | Circulation Research
H, GTP-bound Rap1 protein in mouse neutrophils 30 s after stimulation with CCL5 and prestimulation for 30 minutes with Ac2-26. ... as assessed by measuring the level of GTP-bound Rap1. Thus, Ac2-26 inhibits integrin activation by interfering with the ... Ligands and signaling proteins govern the conformational landscape explored by a G protein-coupled receptor. Proc Natl Acad Sci ... the constitutively active Rap1 fusion protein fully abolished the inhibitory effect of Ac2-26 (Figure 4E; Online Figure IVH), ...
  http://circres.ahajournals.org/content/116/5/827
*  The AF-6 Homolog Canoe Acts as a Rap1 Effector During Dorsal Closure of the Drosophila Embryo | Genetics
2003 Cellular functions of the Rap1 GTP-binding protein: a pattern emerges. J. Cell Sci. 116: 435-440. ... Rap1 binds Cno in a GTP-dependent manner and partially colocalizes with Cno in vivo: To identify molecules through which Rap1 ... Rap1 belongs to the Ras superfamily of small GTPases, which cycle between an inactive GDP-bound and an active GTP-bound state, ... possess Rap1-binding potential and that they interact only with a constitutively active Rap1 mutant, Rap1V12, but not with a ...
  http://www.genetics.org/content/165/1/159
*  Identification and Characterization of Small Molecules as Potent and Specific EPAC2 Antagonists
EPAC is a cAMP-activated guanine nucleotide exchange factor (cAMP-GEF) for the small GTP-binding proteins Rap1 and Rap2.3,4 ... A family of cAMP-binding proteins that directly activate Rap1. Science. 1998;282:2275-2279. [PubMed] ... GTP. guanosine triphosphate. Rap. Ras-related protein. HTS. high-throughput screening. 007-AM. 8-(4-chlorophenylthio)-2'-O- ... Differential binding of cAMP-dependent protein kinase regulatory subunit isoforms Ialpha and IIbeta to the catalytic subunit. J ...
  http://pubmedcentralcanada.ca/pmcc/articles/PMC3574212/?lang=en-ca
*  Rap1 targets VAV2 to circumferential membrane protrusio | Open-i
Rap1 targets VAV2 to circumferential membrane protrusions. (A) Both active and inactive Rap1a localize to membrane protrusions ... rac GTP-Binding Proteins/metabolism*. *rap1 GTP-Binding Proteins/metabolism*. Minor. *Cell Adhesion ... Together, these experiments suggest that active Rap1 relocalizes Rac GEFs that bind to Rap1, such as VAV2, to the periphery of ... Together, these experiments suggest that active Rap1 relocalizes Rac GEFs that bind to Rap1, such as VAV2, to the periphery of ...
  https://openi.nlm.nih.gov/detailedresult.php?img=PMC2172522_200404068f7&req=4
*  KAKEN - Researchers | KATAGIRI Koko (00322157)
Rap1,LFA-1,RAPL,インテグリン,ケモカイン,遊走,ICAM-1,細胞極性,Mst1,chemokine, # of Research Projects:18, # of Research Products:83, Ongoing ... Journal Article] Deficiency of Rap1-GTP binding protein RAPL causes lymphoproliferative disorders through the mislocalization ... Journal Article] Deficiency of Rap1-GTP binding protein RAPL causes lymphoproliferative disorders through the mislocalization ... Presentation] Deficiency of Rap1 binding protein RAPL causes lymphoproliferative disorders through the mislocalization of p27^, ...
  https://nrid.nii.ac.jp/nrid/1000000322157/
*  The GDP Exchange Factor AND-34 Is Expressed in B Cells, Associates With HEF1, and Activates Cdc42 | The Journal of Immunology
Rap-1), and GST-RalBP1 (Ral), respectively. After isolation of the GST-chimeric proteins the GTP-bound GTPases were eluted from ... treatment of WEHI-231 cells with GTP-γ-S demonstrated the ability of the assay to detect endogenous GTP-bound RalA and Rap-1 ( ... Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 18: 578. ... Ras and Rho subfamily GTPases alternate between inactive GDP-bound and active GTP-bound conformations. The ratio of GTPases in ...
  http://www.jimmunol.org/content/170/2/969?ijkey=5e163188919689daab370c4d49dd15de6a53e605&keytype2=tf_ipsecsha
*  Protocols and Video Articles Authored by William C. Mobley (Translated to Hebrew)
We found that NGF induced a sustained activation of Rap1, a small monomeric GTP-binding protein of the Ras family, and that ... Retrolinkin, a membrane protein highly enriched in neuronal endosomes, binds directly to BPAG1n4. Deletion of retrolinkin ... Evidence for Both Neuronal Cell Autonomous and Nonautonomous Effects of Methyl-CpG-binding Protein 2 in the Cerebral Cortex of ... We demonstrate a concomitant reduced expression of SNX27 and CCAAT/enhancer binding protein β (C/EBPβ) in Down's syndrome ...
  https://www.jove.com/author/William+C._Mobley?language=Hebrew
*  RAB27A - Wikipedia
One is the rap1/smg21/Krev-1 protein and the other is a novel GTP-binding protein". J. Biol. Chem. 264 (29): 17000-5. PMID ... The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be ... and characterization of two GTP-binding proteins with molecular weights of 25,000 and 21,000 in human platelet cytosol. ... Cheviet S, Coppola T, Haynes LP, Burgoyne RD, Regazzi R (Jan 2004). "The Rab-binding protein Noc2 is associated with insulin- ...
  https://en.wikipedia.org/wiki/RAB27A
*  THU0021 Defective rap1 activation in synovial fluid t lymphocytes from patients with rheumatoid arthritis | Annals of the...
GTP-bound Rap1 was precipitated using the bacterial expressed fusion protein GST-RalGDS and detected by ECL Western blotting. ... Background Rap1 is a small G-protein, member of the Ras GTPase family. Rap1 has been linked to T cell anergy and it is ... The defective Rap1 activation was not due to increased levels of the Rap1 GTPase activating proteins RapGAP or spa1. While ... This defective activation is not due to increased levels of the Rap1 GTPase activating proteins RapGAP or Spa1. ...
  http://ard.bmj.com/content/60/Suppl_1/A211.3
*  Plus it
Rap1GTP) binds to Ral guanine nucleotide exchange factors (RalGEFs) that activate RalGTPase. RalBP1, one of the Ral GTP-binding ... Scaffolding proteins and actin-binding proteins typically interact with ion channels through conserved, modular protein domains ... Witzgall and co-workers (26) identified Hax-1, a cortactin-binding protein, as a protein interacting with a region between ... The proteins forming the motor protein/actin-regulatory protein complex that facilitate in the intracellular trafficking of ...
  http://ajprenal.physiology.org/content/291/6/F1113
*  Cyclic AMP Raises Intracellular Ca2+ in Human Megakaryocytes Independent of Protein Kinase A | Arteriosclerosis, Thrombosis,...
... bound GTPγS. One of these proteins was identified as Rap1, and GTPγS binding was accompanied by inhibition of Ca2+-ATPases in ... GTP-bound Rap1 to the Rap-binding domain of RalGDS, we investigated the effect of iloprost and forskolin on Rap1 activation. ... The cAMP-elevating agents iloprost, forskolin, and IBMX induce an increase in the active, GTP-bound Rap1 together with Ca2+ ... A family of cAMP-binding proteins that directly activate Rap1. Science. 1998; 282: 2275-2279. ...
  http://atvb.ahajournals.org/content/22/1/179
*  C-Raf - Wikipedia
... like many other small G-protein associating domains) and selectively binds GTP-bound Ras proteins only. (You can see this ... It shows Rap1 in complex with the RBD of c-Raf.) The C1 domain - immediately downstream of the Ras binding domain - is a ... "The small GTP-binding protein, Rhes, regulates signal transduction from G protein-coupled receptors". Oncogene. 23 (2): 559-68 ... most importantly GTP-bound Ras. Activated small G-proteins can thus break up the intramolacular interactions: this results in a ...
  https://en.wikipedia.org/wiki/C-Raf
*  Expression pattern and regulation of genes differ between fibroblasts of adhesion and normal human peritoneum | Reproductive...
D'Silva NJ, Mitra RS, Zhang Z, Kurnit DM, Babcock CR, Polverini PJ, Carey TE: Rap1, a small GTP-binding protein is upregulated ... IGF-binding protein-3-induced growth inhibition and apoptosis do not require cell surface binding and nuclear translocation in ... Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein ... Wu T, Angus CW, Yao XL, Logun C, Shelhamer JH: P11, a unique member of the S100 family of calcium-binding proteins, interacts ...
  https://rbej.biomedcentral.com/articles/10.1186/1477-7827-3-1
*  Nucleotide exchange factor - Wikipedia
Alfred Wittinghofer's seminar: GTP-BINDING PROTEINS AS MOLECULAR SWITCHES. ... "Epac is a Rap1 guanine- nucleotide-exchange factor directly activated by cyclic AMP" (PDF). Nature. 396 (6710): 474-477. doi: ... of nucleoside diphosphates for nucleoside triphosphates bound to other proteins. Many cellular proteins cleave (hydrolyze) ... and are often specific to a single protein or class of proteins with which they interact. Nucleoside-diphosphate kinase Guanine ...
  https://en.wikipedia.org/wiki/Nucleotide_exchange_factor
*  G-protein signaling - RhoA regulation pathway Pathway Map - PrimePCR | Life Science | Bio-Rad
RAP1, GTP-GDP dissociation stimulator 1 (Rap1GDS1) bound with Kinesin-associated protein 3 (KAP3) also activates RhoA [16]. ... that bind to prenylated GDP-bound Rho proteins and allow translocation between membranes and the cytosol [2], [3]. ... thatpromote exchange of GTP for GDP; GTPase-activating proteins (GAPs) that enhance the intrinsic GTP-hydrolysis activity, ... and Rhophilin Rho GTPase binding protein 1 (Rhophilin 1) leads to actin polymerization and cytoskeleton rearrangements, Protein ...
  http://www.bio-rad.com/en-us/prime-pcr-assays/pathway/g-protein-signaling-rhoa-regulation-pathway
*  RAP2A - Wikipedia
"Identification of a specific effector of the small GTP-binding protein Rap2". European Journal of Biochemistry / FEBS. 252 (2 ... "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' ... Ménétrey J, Cherfils J (Nov 1999). "Structure of the small G protein Rap2 in a non-catalytic complex with GTP". Proteins. 37 (3 ... "Identification of a specific effector of the small GTP-binding protein Rap2". European Journal of Biochemistry / FEBS. 252 (2 ...
  https://en.wikipedia.org/wiki/RAP2A
*  Vassiliki Boussiotis | Harvard Catalyst Profiles | Harvard Catalyst
Rap1-GTP-interacting adaptor molecule (RIAM) protein controls invasion and growth of melanoma cells. J Biol Chem. 2011 May 27; ... RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesion. Dev Cell. 2004 Oct; 7(4):585 ... Rap1-GTP is a negative regulator of Th cell function and promotes the generation of CD4+CD103+ regulatory T cells in vivo. J ... Differential association of protein tyrosine kinases with the T cell receptor is linked to the induction of anergy and its ...
  https://connects.catalyst.harvard.edu/Profiles/display/Person/33563
*  Dock4 - Dedicator of cytokinesis protein 4 - Mus musculus (Mouse) - Dock4 gene & protein
Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP ... Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10090. ... Protein-protein interaction databases. STRING: functional protein association networks. More...STRINGi. 10090. ...
  http://www.uniprot.org/uniprot/P59764
*  APBB1IP antibody | acris-antibodies.com
Amyloid beta A4 precursor protein-binding family B member 1-interacting protein, Rap1-GTP-interacting adapter molecule, Proline ... precursor protein-binding, family B, member 1 interacting protein (APBB1IP). Human. > 80 % Preparation: Recombint protein was ... precursor protein-binding, family B, member 1 interacting protein (APBB1IP), 200 uL, ,10^7 TU/mL. Not available. ... precursor protein-binding, family B, member 1 interacting protein (APBB1IP) , 200 uL, ,10^7 TU/mL. Not available. ...
  https://www.acris-antibodies.com/target/apbb1ip-antibody.htm
*  Guanine nucleotide binding properties of rap1 purified from human neutrophils | Biochemical Journal
Furthermore, the ability of a cytosolic rap1 GTPase-activating protein to stimulate neutrophil rap1 GTP hydrolysis was not ... The guanine nucleotide binding properties of rap1 protein purified from human neutrophils were examined using both the protein ... The binding of guanine nucleotides was found to correlate with the loss of endogenous nucleotide from the rap1 protein, which ... Phosphorylation of rap1 in vitro by protein kinase A did not modify any of the observed nucleotide-binding parameters. ...
  http://www.biochemj.org/content/267/2/407
*  Localization of nectin-free afadin at the leading edge and its involvement in directional cell movement induced by platelet...
... for Rap1. In the in vitro binding assay, we used pure recombinant proteins of afadin fused to maltose-binding protein (MBP- ... 5B). As negative controls, His-V12Rap1 did not bind to MBP, nor did His-wild-type Rap1 (Rap1WT), on which GTPγS was not loaded ... B) Direct binding of afadin to active Rap1. His-V12Rap1 bound to MBP-afadin was analyzed by protein staining with Coomassie ... Direct binding of afadin to Rap1. Recombinant maltose-binding protein (MBP) and MBP-fused full-length afadin (MBP-afadin) were ...
  http://jcs.biologists.org/content/122/23/4319
*  Interactive Fly, Drosophila
TSC2 was found to bind to Rheb-GTP in vitro and to reduce Rheb GTP levels in vivo. Over-expression of Rheb but not Rap1 ... These two proteins form a complex. Tuberin, which has a region of homology to Rap1 GTPase-activating proteins (GAPs), has been ... protein databases for optimal substrates of specific protein kinases and for optimal binding motifs for specific protein ... overexpression of Tuberin should switch Rheb from an active GTP bound state to an inactive GDP bound state. To indirectly ...
  http://www.sdbonline.org/sites/fly/newgene/gigas2.htm
*  RAP2B - Wikipedia
1993). "Association of the low molecular weight GTP-binding protein rap2B with the cytoskeleton during platelet aggregation". ... 1988). "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the ' ... Ras-related protein Rap-2b is a protein that in humans is encoded by the RAP2B gene. RAP2B belongs to the Ras-related protein ... a RAS-related GTP-binding protein from platelets". Proc Natl Acad Sci U S A. 87 (17): 6527-31. doi:10.1073/pnas.87.17.6527. PMC ...
  https://en.wikipedia.org/wiki/RAP2B