Antibodies for GTP-bound G Proteins Arf6 Arl1 Arl3 Cdc42 Gα13 Gαi ... Rab5 is a member of the Rab-family GTPases. Rab5 is a rate-limiting component of the machinery regulating the kinetics of ... The bound active Rab5 will then be pulled down by protein A/G agarose. The precipitated active Rab5B will be detected by ... This anti-Rab5-GTP monoclonal antibody can also be used to monitor the activation of Rab5 in cells and in tissues by ...http://www.neweastbio.com/Rab5BAssay.do
Untangling the evolution of Rab G proteins: implications of a comprehensive genomic analysis | BMC Biology | Full Text
In the GTP-bound form, Rabs bind effectors, but when the GTP is hydrolyzed through the action of a GTPase activating protein ( ... Pereira-Leal JB, Seabra MC: Evolution of the Rab family of small GTP-binding proteins. J Mol Biol. 2001, 313: 889-901.PubMed ... and this GTP form then serves as a landmark for the recruitment of those proteins that need to act in that location. Rab-GTP ... Schmitt HD, Wagner P, Pfaff E, Gallwitz D: The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be ...https://bmcbiol.biomedcentral.com/articles/10.1186/1741-7007-10-71
RAB11A - Ras-related protein Rab-11A precursor - Oryctolagus cuniculus (Rabbit) - RAB11A gene & protein
Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set ... That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B ... The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to ... "Enrichment of rab11, a small GTP-binding protein, in gastric parietal cells.". Goldenring J.R., Soroka C.J., Shen K.R., Tang L. ...http://www.uniprot.org/uniprot/P62493
Rab GTPase Regulation of VEGFR2 Trafficking and Signaling in Endothelial Cells | Arteriosclerosis, Thrombosis, and Vascular...
... and ubiquitin-binding effectors. Rabs are Ras-related small GTPases that program vesicle docking and fusion. Rab proteins can ... Expression of GTP or GDP-bound Rab5a mutants block activated VEGFR2 trafficking and degradation. Manipulation of Rab7a GTPase ... Jordens I, Marsman M, Kuijl C, Neefjes J. Rab proteins, connecting transport and vesicle fusion. Traffic. 2005; 6: 1070-1077. ... Expression of the GTP-bound Rab7aQ67L mutant also caused VEGFR2 accumulation in punctate endosomes after prolonged VEGF-A ...http://atvb.ahajournals.org/content/29/7/1119
Multiple Rab GTPase binding sites in GCC185 suggest a model for vesicle tethering at the trans-Golgi. - Oxford Neuroscience
... to the Golgi and for microtubule nucleation at the Golgi via CLASP proteins. GCC185 localizes to the Golgi by cooperative ... Golgi-Rab binding sites may permit GCC185 to contribute to stacking and lateral interactions of Golgi cisternae as well as help ... hybrid and direct biochemical tests that GCC185 contains at least four additional binding sites for as many as 14 different Rab ... A central coiled-coil domain contains a specific Rab9 binding site, and functional assays indicate that this domain is ...https://www.neuroscience.ox.ac.uk/publications/310267
Anti-Rab27A Antibody | Anti-Rab27B Antibody | Rab27A Antibody | Rab27B Antibody
Rab27 is a member of the Rab subfamily of Ras GTPase proteins. Rab27 protein is membrane-bound and is post-translationally ... Rab27A is also known as RAB27A, member RAS oncogene family; GTP-binding protein Ram, RAB27, GS2, RAM, and HsT18676. Rab27B is ... Rab27A: RAB27A, member RAS oncogene family; GTP-binding protein Ram, RAB27, GS2, RAM, HsT18676 ... RNA Extraction and Protein Analysis for Real-Time qPCR * Direct cDNA Synthesis from ...http://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Metabolic_Diseases/Rab27?sitex=10020:22372:US&PEBCL1=cq0dewn0rVAFrds7f6eOhjy8vW&PEBCL1_pses=ZG4E38B5565A326FA821CC8A3B8ED83766B0BD0FD5BDE36DA2749EFFDF887E7D827991C5D6CB44C268B54681A27F97B77B03659B503712BC66
Recombinant Human RAB27A protein (ab159297) | Abcam
Ab159297 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA. Abcam provides free… ... GTP-binding protein Ram. *HsT18676. *MGC117246. *Mutant Ras related protein Rab-27A ... Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family ... Proteins and Peptides. Proteomics tools. Agonists, activators, antagonists and inhibitors. Lysates. Multiplex miRNA assays. By ...http://www.abcam.com/recombinant-human-rab27a-protein-ab159297.html
Rab14 and its exchange factor FAM116 link endocytic recycling and adherens junction stability in migrating cells. - Oxford...
Here, we find that Rab4, Rab11, and Rab14 and the candidate Rab GDP-GTP exchange factors (GEFs) FAM116A and AVL9 are required ... Rab GTPases define the vesicle trafficking pathways underpinning cell polarization and migration. ... Membrane Proteins, Protein Transport, Transferrin, Vesicular Transport Proteins, rab GTP-Binding Proteins, rab4 GTP-Binding ... ADAM Proteins, ADAM10 Protein, Adherens Junctions, Amyloid Precursor Protein Secretases, Biological Transport, Cadherins, Cell ...https://www.neuroscience.ox.ac.uk/publications/332922
Antibodies for GTP-bound G Proteins Arf6 Arl1 Arl3 Cdc42 Gα13 Gαi ... The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal ... The bound active Rab35 will then be pulled down by protein A/G agarose. The precipitated active Rab35 will be detected by ... These anti- Rab35-GTP monoclonal antibodies can also be used to monitor the activation of Rab35 in cells and in tissues by ...http://www.neweastbio.com/Rab35Assay.do
Cellular functions of Rab GTPases at a glance | Journal of Cell Science
Rab effectors. Rab effectors, defined as proteins that interact specifically with the GTP-bound from of a Rab GTPase, come in ... 1998). Distinct Rab-binding domains mediate the interaction of rabaptin-5 with GTP-bound rab4 and rab5. EMBO J. 17, 1941-1951. ... when GTP is bound and 'off' when GDP is bound. Conformational differences between the GDP- and GTP-bound forms mainly involve ... should be reserved for those proteins that interact exclusively with the GTP-bound form of a Rab GTPase. In some cases, ...http://jcs.biologists.org/content/128/17/3171
A Bifunctional Bacterial Protein Links GDI Displacement to Rab1 Activation | Science
Inactive Rab GTPases are maintained in the cytosol by binding to GDP-dissociation inhibitor (GDI). It is believed that ... GDP-GTP) exchange factors (GEFs). Here, we found that SidM from Legionella pneumophila could act as both GEF and GDI- ... specialized proteins are required to displace GDI from Rab GTPases before Rab activation by guanosine diphosphate-guanosine 5′- ... A Bifunctional Bacterial Protein Links GDI Displacement to Rab1 Activation Message Subject. (Your Name) has forwarded a page to ...http://science.sciencemag.org/content/318/5852/974
Antibodies for GTP-bound G Proteins Arf6 Arl1 Arl3 Cdc42 Gα13 Gαi ... Rab7 belongs to the Rab family of small GTPases. Rab7 is located in the late endosome, lysosome, cytoplasmic vesicle, phagosome ... The bound active Rab7 will then be pulled down by protein A/G agarose. The precipitated active Rab7 will be detected by ... These anti- Rab7-GTP monoclonal antibodies can also be used to monitor the activation of Rab7 in cells and in tissues by ...http://www.neweastbio.com/Rab7Assay.do
RAB12 - Ras-related protein Rab-12 - Homo sapiens (Human) - RAB12 gene & protein
Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set ... That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation ... The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to ... while inactivation through hydrolysis of bound GTP is catalyzed by a GTPase activating protein (GAP) (By similarity). That Rab ...http://www.uniprot.org/uniprot/Q6IQ22
RAB3GAP1 - Rab3 GTPase-activating protein catalytic subunit - Homo sapiens (Human) - RAB3GAP1 gene & protein
Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Specifically converts active Rab3-GTP to ... Probable catalytic subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and ... Rab GTPase binding Source: UniProtKB ,p>Inferred from Physical Interaction,/p> ,p>Covers physical interactions between the gene ... Protein. Similar proteins. Organisms. Length. Cluster ID. Cluster name. Size. Q15042. B9A6J2. D3DP75. Homo sapiens (Human). 981 ...http://www.uniprot.org/uniprot/Q15042
Superfamily of plant monomeric GTP-binding proteins: 2. Rab proteins are the regulators of vesicles trafficking and plant...
... mG-proteins). As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key... ... Rab proteins represent the largest family of monomeric GTP-binding proteins ( ... indicating specific for plants functions of Rab proteins.. Key words. plants monomeric GTP-binding proteins Rab proteins ... In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal ...https://link.springer.com/article/10.1134%2FS1021443708010159
Superfamily of plant monomeric GTP-binding proteins: 2. Rab proteins are the regulators of vesicles trafficking and plant...
Rab proteins are the regulators of vesicles trafficking and plant responses to stresses, Russian Journal of Plant Physiology" ... In plants, Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal ... Rab proteins represent the largest family of monomeric GTP-binding proteins (mG-proteins). As distinct from animal cells ... Superfamily of plant monomeric GTP-binding proteins: 2. Rab proteins are the regulators of... Moshkov, I.; Novikova, G. 2011-01 ...https://www.deepdyve.com/lp/springer_journal/superfamily-of-plant-monomeric-gtp-binding-proteins-2-rab-proteins-are-G0jtabvmUT
TBC1D9B has GAP activity in cellula. (A) Localization o | Open-i
GTPase-Activating Proteins/genetics/metabolism*. *rab GTP-Binding Proteins/biosynthesis*/genetics. Minor ... TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. ... TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells. ... This is the expected result for HEK cells if the GDP-bound form of Rab11a increased as a result of expressing TBC1D9B-WT. ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4230784_3779fig9&req=4
"Determinants of the broad recognition of exocytic Rab GTPases by Mss4" by Zhongyuan Zhu, Anna M. Delprato et al.
Mss4, an evolutionarily conserved Rab accessory factor, facilitates nucleotide release and binds tightly to the nucleotide-free ... One of these residues is located at the N-terminus of the switch I region near the nucleotide binding site whereas the other ... A structure-based mutational analysis of residues that are conserved only in exocytic Rab GTPases reveals three residues that ... disposition of these residues with respect to the structure of Rab3A correlates with the dimensions of the elongated Rab ...https://escholarship.umassmed.edu/gsbs_sp/661/
RAB38 Gene - GeneCards | RAB38 Protein | RAB38 Antibody
Protein Coding), RAB38, Member RAS Oncogene Family, including: function, proteins, disorders, pathways, orthologs, and ... Rab-Related GTP-Binding Protein 3 * Ras-Related Protein Rab-38 3 ... RAB GEFs exchange GTP for GDP on RABs. RAB GEFs exchange GTP ... Protein Symbol:. P57729-RAB38_HUMAN. Recommended name:. Ras-related protein Rab-38. Protein Accession:. P57729. Secondary ... Serological cloning of a melanocyte rab guanosine 5'-triphosphate-binding protein and a chromosome condensation protein from a ...https://www.genecards.org/cgi-bin/carddisp.pl?gene=RAB38&keywords=GH11I088188&prefilter=genomic_location
Pea lectin receptor-like kinase functions in salinity adaptation without yield penalty, by alleviating osmotic and ionic...
Role of the Rab GTP-binding protein Ypt3 in the fission yeast exocytic pathway and its connection to calcineurin function ... Heterotrimeric G-protein complex and G-protein-coupled receptor from a legume (Pisum sativum): role in salinity and heat stress ... Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, ... Stress induced beta subunit of heterotrimeric G-proteins from Pisum sativum interacts with mitogen activated protein kinase ...https://www.deepdyve.com/lp/springer_journal/pea-lectin-receptor-like-kinase-functions-in-salinity-adaptation-Cs37gAyo00
"The Legionella pneumophila GTPase activating protein LepB accelerates " by Ashwini K. Mishra, Claudia M. Del Campo et al.
In addition to expanding GTP hydrolytic paradigms, these observations reveal the unconventional dual finger and non-canonical ... from pathogenic bacteria and eukaryotic host organisms deactivate Rab GTPases by supplying catalytic arginine and glutamine ... fingers in trans and utilizing the cis-glutamine in the DXXGQ motif of the GTPase for binding rather than catalysis. Here, we ... catalytic network mechanisms of Rab GAPs as necessary alternative solutions to a major impediment imposed by substitution of ...http://escholarship.umassmed.edu/faculty_pubs/469/
WASH localization is controlled by PI(3,5)P2 levels. (A | Open-i
rab GTP-Binding Proteins/metabolism*. Minor. *Binding Sites. *Cell Line, Tumor. *Endosomes/metabolism ... Mentions: Activation of Arp2/3 complex occurs upon binding of an activator in the WASP family of proteins (Millard et al., 2004 ... Mentions: Activation of Arp2/3 complex occurs upon binding of an activator in the WASP family of proteins (Millard et al., 2004 ... 8). The FAM21 component of WASH has been found to bind multiple phosphoinositides by dot plot, including the major endosomal ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC4555817_JCB_201412127_Fig8&req=4
The CTS is associated with lipid. (A) Comparison of the | Open-i
This suggests that the CTS functions by binding regulatory proteins like Rab8 to control trafficking through the endomembrane ... This suggests that the CTS functions by binding regulatory proteins like Rab8 to control trafficking through the endomembrane ... Bottom Line: This motif is sufficient to target green fluorescent protein (GFP) to cilia of ciliated cells and targets GFP to ... Bottom Line: This motif is sufficient to target green fluorescent protein (GFP) to cilia of ciliated cells and targets GFP to ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2812845_JCB_200910096_RGB_Fig2&req=4
FRET analyses of the interaction between Cav1 and Na/K- | Open-i
rab GTP-Binding Proteins/genetics/metabolism. *rab5 GTP-Binding Proteins/genetics/metabolism ... rab GTP-Binding Proteins/genetics/metabolism. *rab5 GTP-Binding Proteins/genetics/metabolism ... Mentions: We have shown that the Na/K-ATPase can be coimmunoprecipitated with Cav1 and that the purified Na/K-ATPase binds to ... Mentions: We have shown that the Na/K-ATPase can be coimmunoprecipitated with Cav1 and that the purified Na/K-ATPase binds to ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2542476_jcb1821153f04&req=4
Subcellular distribution of wild-type and mutant Rab24 | Open-i
rab GTP-Binding Proteins/chemistry/genetics*/immunology/metabolism*. Minor. *3T3 Cells/chemistry ... Results: The functions of other Rab proteins have been defined through the use of dominant-negative mutants with amino acid ... Results: The functions of other Rab proteins have been defined through the use of dominant-negative mutants with amino acid ... Background: Members of the Rab GTPase family regulate intracellular protein trafficking, but the specific function of Rab24 ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC130051_1471-2121-3-25-1&req=4