Currently, the go-to method for studying microbial eukaryotes is short-read metabarcoding of variable regions of the 18S rRNA ... However, there is a growing interest in applying long-read sequencing of amplicons covering the rRNA operon for improving ... Short- and long-read metabarcoding of the eukaryotic rRNA operon: Evaluation of primers and comparison to shotgun metagenomics ... Keywords: PacBio long-read sequencing; marine plankton; metabarcoding; microbial eukaryotes; primer design; rRNA operon. ...
Fegatella F., Lim J., Kjelleberg S., Cavicchioli R. Implications of rRNA operon copy number and ribosome content in the marine ... Bioluminescent symbionts of the Caribbean flashlight fish (Kryptophanaron alfredi) have a single rRNA operon. 1993. Mol. Mar. ...
Despite this conspicuous deviation from single-copy genes, no phenotype has been consistently associated with rRNA gene copy … ... multiple copies of each rRNA-encoding gene are common on bacterial chromosomes. ... Numbers above bars indicate the rRNA operon copy number for each species; the mean number of rRNA operons per isolate is ... were isolated and characterized for rRNA operon copy number. (B) Mean number of rRNA operons for bacterial isolates from group ...
Determination of the primary structure of fragments of the ribosomal operon of bakers yeast coding for 18 S rRNA (English) ... Determination of the primary structure of fragments of the ribosomal operon of bakers yeast coding for 18 S rRNA. scientific ...
Furthermore, many Fis binding sites occur in tandem such as those in the stable RNA promoters, e.g., P1 promoter of rRNA operon ... In another example, specific binding of HU at the gal operon facilitates the formation of a DNA loop that keeps the gal operon ... MukE and MukF are encoded together with MukB in the same operon in E. coli. Deletion of either subunit results in the same ... DNA bending by IHF differentially controls transcription from the two tandem promoters of the ilvGMEDA operon in E. coli. ...
23S RRNA OPERON DIFFERENCES IN ISOLATES OF CAMPYLOBACTER SPP. - (Abstract Only) Meinersmann, R.J., Phillips, R.W., Ladely, S.R ... 23s rrna operon differences in isolates of campylobacter spp. Campylobacter Helicobacter and Related Organisms International ... 23S rRNA gene mutations contributing to macrolide resistance in Campylobacter jejuni and Campylobacter coli - (Peer Reviewed ... Ribosomal operon intergenic sequence (IGS) heterogeneity in Campylobacter coli and Campylobacter jejuni - (Abstract Only) ...
The target genes for detection include the ATPase operon, P1 and 16S rRNA genes. PCR assays have high specificity and ...
The first operon is located down-stream of the czc operon in cluster K. Genes in this ars operon had high identity (50 to 91%) ... This was confirmed by a phylogenetic analysis based on an intragenic fragment of the 16S rRNA gene (Figure S3). To determine ... 2 czc operons), copper and/or silver (copA, copP, and silA genes), and nickel (1 nre operon and 1 nie operon) tolerance. The ... This operon was present in other Cupriavidus genomes (with the exception of UYPR2.512, where the ars operon is absent), but in ...
45] Two major genetic targets are the Brucella gene BCSP31 and the 16S-23S rRNA operon. The 16S-23S rRNA operon has been shown ...
Bosch, L.; Nilsson, L.; Vijgenboom, E.; Verbeek, H. FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli ... First of all, genomic transcription turned out to be both pervasive and fuzzy, so that the operon model does not hold the ... Appleman, J.A.; Ross, W.; Salomon, J.; Gourse, R.L. Activation of Escherichia coli rRNA transcription by FIS during a growth ... Zhang, X.; Bremer, H. Effects of Fis on ribosome synthesis and activity and on rRNA promoter activities in Escherichia coli. J ...
We also evaluate the degree of coordination of transcription within archaeal operons and make the unexpected observation that ... volcanii operons that include rRNA genes [60]. Figure 5A shows KAS-seq and ATAC-seq profiles along rRNA genes in the different ... internal operon peaks are observed for multiple operons, while there are also other operons where KAS-seq signal is more ... For the purpose of examining repetitive regions in the genome, such as the rRNA operons, which exist in two identical copies in ...
... revealed by molecular phylogenetic analyses of complete nuclear and plastid-encoded rRNA operons. Protist 163, 778-805 (2012). ... analysis of nuclear 18S rRNA sequences. Mol. Biol. Evol. 14, 56-68 (1997). ...
Boer, H. A. de, Gilbert, S. F., and Nomura, M. (1979). DNA sequences of promoter regions for rRNA operons rrnE and rrnA in ... Identification of initiation sites for the in vitro transcription of rRNA operons rrnE and rrnA in Escherichia coli. Cell 17: ...
Data are expressed as (log2) fold changes (normalized to 18S rRNA) relative to vehicle control. ... Eurofins MWG Operon Oligos) used for qPCR analysis are listed in Table S1. Expression levels were calculated using the delta- ...
... differing in one thymine deletion at position 1,449 of the 16S rRNA gene. Nevertheless, in both rRNA operons allele Mxe-C were ... but sequence of the rRNA operons shifted due one nucleotide deletion in one 16S rRNA gene. nt = not typeable. ... but during the doubled stranded sequencing it became obvious that the isolates possess two rRNA operons, ... After four weeks grown mycobacteria were differentiated by DNA sequencing 16S rRNA, the hsp65 as well rpoB genes (Telenti et al ...
2,420 predicted protein-encoding genes, 67 tRNA genes, 8 rRNA operons, and 1 single 5S rRNA gene. The plasmid (. 31.53% G+C ... 2,420 predicted protein-encoding genes, 67 tRNA genes, 8 rRNA operons, and 1 single 5S rRNA gene. The plasmid contained a G+C ... 8 rRNA operons, and 1 single 5S rRNA gene. The plasmid contained a G+C content of 31.53% which housed 54 protein-encoding genes ... Wallbanks, S., A. J. Martinez-Murcia, J. L. Fryer, B. A. Phillips, and M. D. Collins.(1990). "16S RRNA Sequence Determination ...
The NCCP11945 genome encodes 54 tRNAs and four copies of 16S-23S-5S rRNA operons.[10] ...
2015) Variability of rRNA operon copy number and growth rate dynamics of Bacillus isolated from an extremely oligotrophic ... The 16S rRNA gene analysis was done as previously reported (Avitia et al., 2014; Alcaraz et al., 2016). Briefly sequencing ... Dendrogram of the 1284 strains of the genus Bacillus reconstructed from 16 s rRNA.. All taxa have a sequence divergence over 97 ... 2011) Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons Genome Research 21:494- ...
50 containing an rRNA operon (rrnB) from E. coli (Bruce, 1996). The riboprint pattern of restriction fragments was converted to ... The membrane was hybridized with a chemiluminescent labeled probe containing an rRNA operon (rrnB) from E. coli (Bruce, 1996). ... Numerical analysis of 16S rRNA by EcoRI. Clusters show the patterns of all types obtained. Numerical analysis of the 29 ... Numerical analysis of 16S rRNA by EcoRI. Clusters show the patterns of all types obtained. Numerical analysis of the 26 ...
Manual inspection of the assembly graph using Bandage showed a closed bi-circular representation, with the rRNA operon forming ... However, MAGs often lack SSU rRNA sequences, as observed in several recent studies (1, 24). The SSU rRNA gene itself is ... Assembly graph and SSU rRNA-based binning of microbial genomes. The SSU rRNA gene is an essential tool in microbial ecology, ... With such a filtered database, the general-purpose mapper BBmap extracts SSU rRNA reads five times faster than the rRNA- ...
More than 1.8 million raw operon reads were obtained from the participants with ∼600,000 rRNA reads passing quality assurance/ ... Bacterial ribosomal operons were sequenced using the Oxford Nanopore MinION to determine the relative abundance of bacterial ... quality control (70-95% identify; >1,200 bp alignment) by Discontiguous MegaBLAST against the EZ BioCloud 16S rRNA gene ...
Natural Chromosome-Chromid Fusion across rRNA Operons in a Burkholderiaceae Bacterium. Mori JF, Kanaly RA. Mori JF, et al. ...
D27.505.259.750.600.825 rRNA Operon G5.360.340.300.500.817 G5.360.340.358.207.500.817 Russia Z1.252.122.500 Safrole J2.500. ... C23.888.592.612.451 Lac Operon G5.360.340.300.500.545 G5.360.340.358.207.500.545 Laryngismus C8.360.480 C8.360.895.500 C8.618. ... G5.360.340.358.207.500.645 Operon G5.360.340.300.500 G5.360.340.358.207.500 Ophthalmic Solutions D27.505.954.578.645 Opioid- ...
Sequencing and analysis of fungal rRNA operons for development of broad-range fungal PCR assays. Appl Environ Microbiol. 2009; ...
Ribosomal ribonucleic acid operon Current Synonym true false 49438019 RRNA operon Current Synonym true false ...
Operon, rRNA. Operon, rrnB. Operons, rRNA. Operons, rrnB. rRNA Operons. rrnB Operon. rrnB Operons. ... rRNA Operon - Preferred Concept UI. M0019286. Scope note. Genetic loci which direct transcription of ribosomal RNA in bacterial ... Operon, rRNA Operons, rRNA rRNA Operons rrnB Operon - Narrower Concept UI. M0019287. ... Genetic loci which direct transcription of ribosomal RNA in bacterial operons. They are designated rrnB, rrnC, rrnD, etc. ...
Microbial Identification Using rRNA Operon Region: Database and Tool for Metataxonomics with Long-Read Sequence.. Seol D; Lim ... Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis.. Johnson JS; Spakowicz DJ; Hong BY; ... 7. Combining 16S rRNA gene variable regions enables high-resolution microbial community profiling.. Fuks G; Elgart M; Amir A; ... Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing.. Shin J; Lee S; Go MJ; Lee SY; Kim SC; Lee ...