Uptake kinetics of cell-penetrating peptides. - Matthew Woods Group
As our knowledge increases about the diversity in uptake mechanisms displayed by cell-penetrating peptides (CPP), the concept ... Cell-Penetrating Peptides, Fluorescent Dyes, HeLa Cells, Humans, Intracellular Space, Kinetics, Protein Transport, Spectrometry ... As our knowledge increases about the diversity in uptake mechanisms displayed by cell-penetrating peptides (CPP), the concept ...
Delivery of therapeutic oligonucleotides with cell penetrating peptides - Matthew Woods Group
Cell-penetrating peptides (CPPs) appear as a possibility to increase the cellular delivery of non-permeant biomolecules such as ... Cell-penetrating peptides (CPPs) appear as a possibility to increase the cellular delivery of non-permeant biomolecules such as ... Delivery of therapeutic oligonucleotides with cell penetrating peptides Boisguérin P., Deshayes S., Gait MJ., ODonovan L., ...
Prevention of exercised induced cardiomyopathy following Pip-PMO treatment in dystrophic mdx mice. - Department of Paediatrics
Repeated peptide-AO treatments resulted in high levels of cardiac dystrophin protein, which prevented the exercised induced ... This work demonstrates that high levels of cardiac dystrophin restored by Pip peptide-AOs prevents further deterioration of ... Pip peptide-AOs demonstrate high activity in cardiac muscle. To determine their therapeutic value, dystrophic mdx mice were ... Pip peptide-AOs demonstrate high activity in cardiac muscle. To determine their therapeutic value, dystrophic mdx mice were ...
In vivo biodistribution and efficacy of peptide mediated delivery. - Matthew Woods Group
Since the late 1990s, cell-penetrating peptides (CPPs) have been utilized as transport vectors for a broad spectrum of ... Since the late 1990s, cell-penetrating peptides (CPPs) have been utilized as transport vectors for a broad spectrum of ... Cell-Penetrating Peptides, Drug Carriers, Humans, Inflammation, Neoplasms, Oligonucleotides, Protein Transport, Proteins, RNA ...
Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides. - Matthew Woods Group
We show here that lipid-functionalized cell-penetrating peptides can be efficiently exploited for cellular transfection of the ... peptides, liposomes, or similar nanoparticle delivery systems. However, due to the lack of electrostatic interactions, this ... study to show delivery through complex formation of biologically active charge-neutral oligonucleotides by cationic peptides. ... Peptide nanoparticle delivery of charge-neutral splice-switching morpholino oligonucleotides. Järver P., Zaghloul EM., ...
Broad and strong memory CD4+ and CD8+ T cells induced by SARS-CoV-2 in UK convalescent individuals following COVID-19. -...
We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ ... epitopes were defined, with peptide-MHC pentamer-positive cells displaying the central and effector memory phenotype. In mild ... using interferon-γ-based assays with peptides spanning SARS-CoV-2 except ORF1. The breadth and magnitude of T cell responses ... We identified 41 peptides containing CD4+ and/or CD8+ epitopes, including six immunodominant regions. Six optimized CD8+ ...
Genome-wide effects of the antimicrobial peptide apidaecin on translation termination - Department of Paediatrics
... inhibits protein synthesis by binding in the nascent peptide exit tunnel and trapping the release factor associated with a ... AbstractBiochemical studies suggested that the antimicrobial peptide apidaecin (Api) ... Genome-wide effects of the antimicrobial peptide apidaecin on translation termination Mangano K., Florin T., Shao X., Klepacki ... AbstractBiochemical studies suggested that the antimicrobial peptide apidaecin (Api) inhibits protein synthesis by binding in ...
Characterization of the antibody response elicited by immunization with pneumococcal surface protein A (PspA) as recombinant...
On the other hand, antibodies induced by the DNA vaccine showed reactivity with only two peptides. Though both strategies were ... alum showed positive reaction with several synthetic peptides, mostly located in the first half of the protein. ... On the other hand, antibodies induced by the DNA vaccine showed reactivity with only two peptides. Though both strategies were ... alum showed positive reaction with several synthetic peptides, mostly located in the first half of the protein. ...
Context Dependent Effects of Chimeric Peptide Morpholino Conjugates Contribute to Dystrophin Exon-skipping Efficiency. -...
Four additional chimeric peptide-PMO conjugates including newly identified peptide 9 (B-9-PMO and 9-B-PMO) and control peptide ... and novel chimeric peptides containing CPP (referred as B peptide) and muscle-targeting peptide (referred as MSP) motifs ... Collectively, our data demonstrate that peptide orientation in chimeric peptides is an important parameter that determines ... peptide-PMO conjugates is dependent on relative position of the tissue-targeting peptide motif within the chimeric peptide with ...
Peptide-conjugated antimiRs improve myotonic dystrophy type 1 phenotypes by promoting endogenous MBNL1 expression. - Department...
... chemistry conjugated to cell-penetrating peptides (CPPs) to improve delivery to affected tissues. In DM1 cells, CPP-PMOs ... Peptide-conjugated antimiRs improve myotonic dystrophy type 1 phenotypes by promoting endogenous MBNL1 expression. ... Peptide-conjugated antimiRs improve myotonic dystrophy type 1 phenotypes by promoting endogenous MBNL1 expression. ... chemistry conjugated to cell-penetrating peptides (CPPs) to improve delivery to affected tissues. In DM1 cells, CPP-PMOs ...
Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic...
One of the lead peptide-AO conjugates, Pip5e-AO, showed highly efficient exon skipping and dystrophin production in mdx mice ... Here, we report identification of a series of transduction peptides (Pip5) as AO conjugates for enhanced systemic and ... Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic ... Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic ...
Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic...
One of the lead peptide-AO conjugates, Pip5e-AO, showed highly efficient exon skipping and dystrophin production in mdx mice ... Here, we report identification of a series of transduction peptides (Pip5) as AO conjugates for enhanced systemic and ... Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic ... Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic ...
Molecular correction of Duchenne muscular dystrophy by splice modulation and gene editing. - Matthew Woods Group
Second-generation exon skipping drugs (i.e. peptide-antisense oligonucleotide conjugates) exhibit enhanced potency, and also ... Second-generation exon skipping drugs (i.e. peptide-antisense oligonucleotide conjugates) exhibit enhanced potency, and also ...
T-cell responses against meningococcal antigens. - Department of Paediatrics
... and recycling of MHC-peptide complexes to the surface of APCs. Whereas CD8+ T cells recognize endogenously derived antigen ( ... T-cells recognize protein antigens as short peptide fragments (8-20 amino acids) bound to major histocompatibility complex (MHC ... T-cells recognize protein antigens as short peptide fragments (8-20 amino acids) bound to major histocompatibility complex (MHC ... and recycling of MHC-peptide complexes to the surface of APCs. Whereas CD8+ T cells recognize endogenously derived antigen ( ...
HLA-specific intracellular epitope processing shapes an immunodominance pattern for HLA-B*57 that is distinct from HLA-B*58:01....
Here we show that HLA-B*58:01 binds and presents KF11 peptide, but HIV-infected HLA-B*58:01(+) cells fail to process KF11. This ... Here we show that HLA-B*58:01 binds and presents KF11 peptide, but HIV-infected HLA-B*58:01(+) cells fail to process KF11. This ... CD8-Positive T-Lymphocytes, Epitopes, HIV Infections, HIV-1, HLA-B Antigens, Humans, Peptide Fragments, T-Lymphocytes, ...
Differences in the expressed HLA class I alleles effect the differential clustering of HIV type 1-specific T cell responses in...
... were targeted with similar frequency in both populations and all study subjects recognized at least one overlapping peptide. ... in 32 HIV-1-infected individuals of Chinese origin and compared to 34 infected caucasians using 410 overlapping peptides ... were targeted with similar frequency in both populations and all study subjects recognized at least one overlapping peptide. ... in 32 HIV-1-infected individuals of Chinese origin and compared to 34 infected caucasians using 410 overlapping peptides ...
HLA-B*57 Micropolymorphism shapes HLA allele-specific epitope immunogenicity, selection pressure, and HIV immune control. -...
HLA-B*57 alleles and the closely related HLA-B*5801 are often grouped together because of their similar peptide-binding motifs ... HLA-B*57 alleles and the closely related HLA-B*5801 are often grouped together because of their similar peptide-binding motifs ...
The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected...
57 HIV-1-infected individuals were screened for responses against Tat and Rev by using overlapping peptides spanning the entire ... Peptides, T-Lymphocytes, Cytotoxic, rev Gene Products, Human Immunodeficiency Virus, tat Gene Products, Human Immunodeficiency ... 57 HIV-1-infected individuals were screened for responses against Tat and Rev by using overlapping peptides spanning the entire ...
Extracellular vesicle-mediated delivery of molecular compounds into gametes and embryos: learning from nature. - Department of...
... cell-penetrating peptides, sperm, oocyte, egg, embryo, exosomes, microvesicles, extracellular vesicles, ... Cell-Penetrating Peptides, Drug Delivery Systems, Embryo, Mammalian, Extracellular Vesicles, Female, Humans, Male, ... cell-penetrating peptides, sperm, oocyte, egg, embryo, exosomes, microvesicles, extracellular vesicles, ...
The acute inflammatory response to intranigral α-synuclein differs significantly from intranigral lipopolysaccharide and is...
Amyloid beta-Peptides, Animals, Cell Death, Cell Line, Cytokines, Female, Inflammation, Interleukin-1beta, Lipopolysaccharides ...
Human leukocyte antigen alleles associate with COVID-19 vaccine immunogenicity and risk of breakthrough infection. - Department...
We identified a distinct spike-derived peptide that is predicted to bind differentially to HLA-DQB1*06 compared to other ... We identified a distinct spike-derived peptide that is predicted to bind differentially to HLA-DQB1*06 compared to other ...
From gut to brain: bioencapsulated therapeutic protein reduces amyloid load upon oral delivery. - Matthew Woods Group
Peptides, Plaque, Amyloid, Serine Proteases ...