A dual role for Rac1 GTPases in the regulation of cell motility | Journal of Cell Science
Deactivation of small GTPases is also attributed to a less numerous group of guanine nucleotide dissociation inhibitors (GDIs) ... 2005). GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors. Nat. Rev. Mol. Cell Biol. 6, 167-180. ... Small GTPases are activated by guanine nucleotide exchange factors (GEFs) and deactivated by GTPase-activating proteins (GAPs ... It might be in a GTP-bound or a GDP-bound form, or might even assume a nucleotide-free form as shown for Cdc42 in a complex ...
Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration | Journal of Cell Science
2010). The connecdenn family, Rab35 guanine nucleotide exchange factors interfacing with the clathrin machinery. J. Biol. Chem. ... glycerol supplemented with protease inhibitors. Lysates were centrifuged for 5 minutes at 4°C at 205,000 g. A 4 mg aliquot of ... C to invoke cadherin internalization and cell dissociation. Cells were then pelleted to removed EDTA and resuspended in DMEM ... Once activated by guanine nucleotide exchange factors (GEFs), GTP-bound Arfs and Rabs recruit a plethora of effectors that have ...