The prion protein inhibits monocytic cell migration by stimulating β1 integrin adhesion and uropod formation | Journal of Cell...
FN, fibronectin. (D) PMA-stimulated adhesion of shCtrl- and shPrP-U937 to BSA and fibronectin was determined by dividing the ... Human fibronectin was from Biopur. Recombinant human Fc-ICAM and Fc-VCAM were from R&D Systems. The APC-labeled polyclonal goat ... Cell adhesion to fibronectin, BSA, Fc-VCAM or Fc-ICAM was measured by using the electrical cell-substrate impedance sensing ... 2C). shCtrl-U937 cells seeded on fibronectin-coated electrodes showed a larger increase in PMA-stimulated adhesion than shPrP- ...
N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading | Journal of Cell Science
2006). Fibronectin rigidity response through Fyn and p130Cas recruitment to the leading edge. Mol. Biol. Cell 17, 2684-2695. ... Cells were pre-incubated in DMSO or 5 µM of FAK inhibitor PF-228 for 30 min before being plated on fibronectin and being ... Cells were pre-incubated in 20 µM PP2 or its control chemical PP3 for 30 min before being plated on fibronectin-coated dishes ( ... Cells were plated on fibronectin for 10 min before being fixed and stained for N-WASP and pY165 Cas. Scale bars: 10 µm. ...
Ca2+ and calpain control membrane expansion during the rapid cell spreading of neutrophils | Journal of Cell Science
... fibronectin-coated glass substrates and (C) in the presence of Ni2+ (1 mM) which blocks Ca2+ influx are shown as a series of ...
pp60c-src and related tyrosine kinases: a role in the assembly and reorganization of matrix adhesions | Journal of Cell Science
Schlaepfer, D. D., Broome, M. A. and Hunter, T. ( 1997). Fibronectin-stimulated signaling from a focal adhesion kinase-c-Src ... Hirst, R., Horwitz, A., Buck, C. and Rohrschneider, L. ( 1986). Phosphorylation of the fibronectin receptor complex in cells ... Cells were plated on glass coverslips precoated with 25 μg/ml bovine plasma fibronectin (Sigma). The cells were simultaneously ... The cells were plated on fibronectin-coated cover glasses for 15 minutes or 24 hours, permeabilized, fixed and immunolabeled ...
Antibodies binding the ADAM10 substrate recognition domain inhibit Eph function | Journal of Cell Science
Cells were detached and plated onto fibronectin-coated glass coverslips for fluorescence microscopy. Representative images from ... and plated onto coverslips pre-coated with fibronectin and stripes of alexa594-labelled ephrin-A5-Fc. As a comparison, cells ... Coverslips were then coated with fibronectin (Sigma) at 10 µg/ml in PBS. EphrinA5-Fc labelled with Alexa594 (Invitrogen) at 16 ...
Binding of soluble fibronectin to integrin α5β1 - link to focal adhesion redistribution and contractile shape | Journal of Cell...
Soluble fibronectin binding to α5β1 integrin supports Rho GTP-loading independent of ROCK or myosin-II activity or fibronectin ... C) Assembled fibronectin-biotin on GE11 cells expressing indicated β1 integrin subunits; fibronectin is red and nuclei are blue ... C) Images of assembled fibronectin-biotin on Itgb3-knockout and wild-type MEFs; fibronectin is red and nucleus blue in merged ... B) Images of assembled fibronectin-biotin on GE11 cells expressing β1 or β1-3-1; fibronectin is red and nucleus blue. Scale bar ...
Non-Smad TGF-β signals | Journal of Cell Science
Hocevar, B. A., Brown, T. L. and Howe, P. H. (1999). TGF-β induces fibronectin synthesis through a c-Jun N-terminal kinase- ... Fibronectin gene expression was originally shown to depend on JNK signals activated by TGF-β in a Smad-independent manner ( ...
Phosphorylation of paxillin tyrosines 31 and 118 controls polarization and motility of lymphoid cells and is PMA-sensitive |...
... cells to collagen and rat ascites hepatoma MM1 cells to fibronectin in the presence of lysophosphatidic acid (Petit et al., ... was phosphorylated upon adherence of primary chicken embryo fibroblasts to fibronectin (Schaller and Schaefer, 2001). Similar ... that in rat ascites hepatoma MM1 cells phosphorylation of tyrosine 181 is constitutive and not augmented by fibronectin and ... tyrosines 31 and 118 were the major phosphorylation sites during adhesion of primary chicken embryo fibroblasts to fibronectin ...
Motility and substratum adhesion of Dictyostelium wild-type and cytoskeletal mutant cells: a study by RICM/bright-field double...
1993). Maximal migration of human smooth muscle cells on fibronectin and type IV collagen occurs at an intermediate attachment ...
Palladin promotes assembly of non-contractile dorsal stress fibers through VASP recruitment | Journal of Cell Science
D) An example of a VASP knockdown (KD) cell that had been plated on a crossbow-shaped fibronectin micropattern, stained for ... After transient transfection, the cells were incubated for 24 h and re-plated before imaging on 10-µg/ml fibronectin-coated ... D) Control (top row) and palladin knockdown (pld KD, bottom row) cells plated on crossbow-shaped fibronectin micropatterns were ... 2), the VASP knockdown cells that had been plated on crossbow-shaped fibronectin micropatterns exhibited transverse arcs and ...
Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin beta1A. Requirement for...
On the other hand, the TT788-9AA mutant, was defective in mediating cell attachment and did not contribute to fibronectin ... formation of fibronectin fibrils, and activation of focal adhesion kinase. Thus, phosphorylation of these residues is not ...
Electrical dimensions in cell science | Journal of Cell Science
... up-regulated expression and asymmetric redistribution of the epidermal growth factor receptors and is enhanced by fibronectin ...
Integrin-linked kinase and PINCH: partners in regulation of cell-extracellular matrix interaction and signal transduction |...
... including upregulation of integrin-mediated fibronectin matrix assembly and downregulation of cell-cell adhesions. Furthermore ...
Regulation of BMP-dependent chondrogenesis in early limb mesenchyme by TGFβ signals | Journal of Cell Science
... stimulation of mesenchymal condensation and fibronectin gene expression by exogenous TGF-beta and evidence for endogenous TGF- ...