The Immune Epitope Database (IEDB): 2018 update. Nucleic Acids Res. 2019;47(d1):D339-D343.. View this article via: CrossRef ... BCG strains differ in their predicted T cell epitopes, gene expression, and virulence toward immune-deficient mice, among other ... sublineages with broader global distribution and greater variability in T cell epitopes than the more geographically confined " ...

The N4/int-3 construct encodes 1 HA epitope at the 3′ end. The Ad-N1IC encodes the cytoplasmic domain of human Notch1, ...

Three type-specific epitopes were identified, two of them in highly variable regions of envelope. In longitudinal studies in ... Eight of the epitopes identified were group specific, lying in relatively conserved regions of Gag, reverse transcriptase, and ... with a progressive increase in the number of CTL epitopes recognized by this subject over time. Our data demonstrate that type- ... responses make up a significant proportion of the host cellular immune response against HIV-1 and that a broadening of epitope ...

C and G) Immunoblots of cell lysates with antibodies against the myc epitope. (D and H) Quantifications of myc as percentage of ... and immunostaining with antibodies against myc epitope tag on the expressed α-synuclein proteins was performed to quantify the ... and then heat-induced epitope retrieval was performed with citrate-based antigen unmasking solutions (Vector Laboratories). ...

We conclude, therefore, that tubular basement membrane target epitopes and their paratypic recognition are highly conserved ...

Adenoviral epitope-tagged PGC-1 mRNA is denoted myc-PGC-1 and endogenous PGC-1 transcripts are labeled endogenous PGC-1. The α- ... Infection of the cardiac myocytes with an adenoviral vector designed to express a myc epitope-tagged PGC-1 (Ad-PGC-1) resulted ... the GFP gene and the PGC-1 cDNA containing a carboxy-terminal myc epitope tag downstream of separate cytomegalovirus (CMV) ...

It has been demonstrated that antibodies generated against the known epitope required infusion of angiotensin II to ...

For immunohistochemistry, epitopes were unmasked by incubation with sodium citrate, pH 6.0, and incubated with primary ...

LECs were the only subset to express the PTA tyrosinase epitope Tyr369, a major immunotherapy target for melanoma (60, 66). If ...

The role of immune responses to previously seen endemic coronavirus epitopes in severe acute respiratory coronavirus 2 (SARS- ... Fatal COVID-19 outcomes are associated with an antibody response targeting epitopes shared with endemic coronaviruses. ... Fatal COVID-19 outcomes are associated with an antibody response targeting epitopes shared with endemic coronaviruses. ... is that the immune response to the SARS-CoV-2 spike protein is enriched for antibodies directed against epitopes shared with ...

With the exception of a mAb that bound to the HA globular head, all heterosubtypic mAbs bound to acid-sensitive epitopes in the ...

Epitope-specific antibodies were used for IHC detection of F4/80, αSMA, FasL, and IRF5 staining (Supplemental Table 5). For IHC ... For nuclear stains and cytoplasmic epitopes, cells were then stained with appropriate fluorochrome-conjugated antibodies in ... Cells were then blocked and washed by centrifugation before staining surface and intracellular epitopes. See Supplemental Table ...

Pathogenic CD4 T cells in type 1 diabetes recognize epitopes formed by peptide fusion. Science. 2016;351(6274):711-714.. View ...

Quantification of Ki67-stained cells (antibody epitope reacts with human Ki67 protein) in mouse lung sections from shNT-, sh18 ...

These multi-epitope vaccines increased memory CD8+ T cells that produced IFN-γ and protected mice against parasite burden when ... Multi-epitopes adjuvanted with GLA-SE are captured and presented by MHC molecules on the APCs to T lymphocytes. ... Multi-epitope proteins with GLA-SE are captured by the Antigen-presenting cells (APCs), and the peptides contained are ... Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii. ...

... binding to linear epitopes on both canonical and TI segments of the TI variants. The removal of cationic proteins from bovine ...

A fragment containing the human cDNA for Rab27a or Rab27b fused to a myc-epitope was generated by PCR and subcloned into the ...

Domain 2 of the urokinase receptor contains an integrin-interacting epitope with intrinsic signaling activity: generation of a ...

... but they can actually be measured in the circulation using antibodies directed against oxidation-specific epitopes. For example ...

The single epitope vector had little effect, but the dual-epitope vectors significantly decreased expression (Figure 5C). ... LCMV MHC class I epitope GP33-41, and LCMV MHC class II epitope GP66-77 were shown. SAMHD1-KO mice were infected with 3 × 106 ... LCMV MHC class I epitope GP33-41, and LCMV MHC class II epitope GP66-77; PD-1-CD40L-GP33.GP66 expressing PD-1 microbody, CD40L ... The effect was more pronounced by the inclusion of an MHC class II-restricted epitope in the vaccine vector together with a PD- ...

T-cell responses directed against multiple HLA-A*0201-restricted epitopes derived from Wilms tumor 1 protein in patients with ...

... targeted linear and conformational epitopes within RH5, and inhibited key interactions within the RH5 invasion complex. This is ...

Heat-induced epitope retrieval of deparaffinized sections was with EnVision FLEX Target Retrieval Solution, Low pH (Dako Omnis ...

... reduces the scope of the BCR repertoire to microbial pathogens and that cross-reactivity between foreign and self epitopes may ...

... that are also reactive against SARS-CoV-2 epitopes (16, 17). The SARS-CoV-2 epitopes recognized by these T cells share ... 02*01 immunodominant epitopes of SARS-CoV-2 (Supplemental Table 1) and IAV M1 epitope (GILGFVFTL) were passively loaded onto ... In this study, we focused on the cross-reactivity of selected SARS-CoV-2 CD8+ T cells with T cells specific for M1, an epitope ... To validate our approach, the LLL epitope was selected to expand SARS-CoV-2 and IAV cross-reactive T cells. This was done using ...

Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope. Science. 1985; ... including the DEK epitope (38), as compared with the SalI sequence used in the current clinical vaccines (29, 37). Like PvDBP ...

Exposure of epitope residues on the outer face of the Chikungunya virus envelope trimer determines antibody neutralizing ... Epitope specificity plays a critical role in regulating antibody-dependent cell-mediated cytotoxicity against influenza A virus ... Early neutralizing IgG response to Chikungunya virus in infected patients targets a dominant linear epitope on the E2 ... Neutralizing monoclonal antibodies block Chikungunya virus entry and release by targeting an epitope critical to viral ...

A malaria vaccine candidate based on an epitope of the Plasmodium falciparum RH5 protein. Malar J. 2014;13:326. View this ... it cannot be ruled out that the induced antibodies could bind to a different epitope not present in AMA-1. Clearly, downstream ...

Glycoprotein 96 can chaperone both MHC class I- and class II-restricted epitopes for in vivo presentation, but selectively ...

... antigen retrieval was performed using R-UNIVERSAL Epitope Recovery Buffer (EMS, 62719-20) in a pressure cooker for 15 minutes ...