Cryopreservation. * To harvest the Toxoplasma culture, detach any remaining tissue culture cells (infected and uninfected) by ... Dispense in 0.5 mL aliquots to 1.0-2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). ...
Cryopreservation. *Harvest cells from a culture that is at or near peak density. To detach cells from the wall of the culture ... Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation ...
Cryopreservation. 1. To achieve the best results set up cultures with several different inocula (e.g. 0.25 ml, 0.5 ml, 1.0 ml ...
Cryopreservation. 1. Harvest cells from a culture that is at or near peak density by centrifugation at ~800 x g for 5 min. Pool ...
Cryopreservation. For a brief overview of the cryopreservation procedure for organoids see our quickstart guide Organoid ... Reagents for cryopreservation. We recommend cryopreserving this model in ATCC Stem Cell Freezing Media (ATCC ACS-3020) ... Cryopreservation Protocol.. For more details on the handling and culture of organoids see our methods paper in Current ...
Cryopreservation. Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium ...
Reagents for cryopreservation. Supernatant supplemented with 7.5% (v/v) DMSO (ATCC 4-X) ...
Reagents for cryopreservation. Complete growth medium supplemented with 10% (v/v) DMSO (ATCC 4-X) ...
Reagents for cryopreservation. Complete growth media containing 10% DMSO (ATCC 4-X) ...
Reagents for cryopreservation. Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) ...
Reagents for cryopreservation. Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) ...
Reagents for cryopreservation. Complete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X) ...
Vials contain at least 500,000 cells in one mL cryopreservation solution. Handling information. ...
Nearly 20 yearslater, cryopreservation and liquid nitrogen storage was introduced at ATCC. Since then, our laboratory experts ...
This essential laboratory process covers the thaw, growth, propagation, and cryopreservation of cells that have been isolated ... Best practices for reviving cells from cryopreservation are critical to ensuring unambiguous experimental results in basic ... and cryopreservation of continuous cell lines, primary cells, hTERT-immortalized primary cells, and organoids. ...