"The inhibition of brain hexokinase by adenosinediphosphate and sulfhydryl reagents". Journal of Biological Chemistry 206, 1954 ... will] develop a chemical process to achieve synthesis of a new class of Sodium Glucose Cotransporters inhibitors (...), for the ... He demonstrated that neither the phosphorylation-dephosphorylation mechanism nor other covalent reactions accounted for glucose ... Furthermore, major pharmaceutical companies are developing inhibitors of the Na/glucose cotransporters to treat diabetes and ...

The terminal sulfhydryl group of the phosphopantetheine is the site of attachment of the growing polyketide chain. Located at ... Functions via catalyzing acyl transfer from methylmalonyl-CoA to the ACP domain within the same module via a covalent acyl-AT ... is a much more practical method than synthesizing each product via chemical reactions in vitro due to the cost of reagents and ... length remains unknown but it is expected that ACP-bound fatty acid chains of the correct length act as allosteric inhibitors ...

This cysteine residue contains a sulfhydryl functional group which allows the peptide to be easily conjugated to a carrier ... Immobilized metal ion affinity chromatography (IMAC) is based on the specific coordinate covalent bond of amino acids, ... and eliminates handling of toxic reagents. Amino acid media is used with a variety of serum proteins, proteins, peptides, and ... "Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate-based cholera toxin inhibitors". ...

This anion then quickly breaks a disulfide bond of Cys58 - Cys63, forming a short lived covalent bond a stable charge-transfer ... There are two main classes of GR targeting compounds: Inhibitors of GSSG binding, or dimerization: Reactive electrophiles such ... Glutathione reductase (EC 1.8.1.7) catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form glutathione ( ... or the formed GSH can be visualized by Ellmans reagent. Alternatively the activity can be measured using roGFP (redox- ...

Not all irreversible inhibitors form covalent adducts with their enzyme targets. Some reversible inhibitors bind so tightly to ... The residues modified are those with side chains containing nucleophiles such as hydroxyl or sulfhydryl groups; these include ... Lundblad RL (2004). Chemical reagents for protein modification (3rd ed.). CRC Press. ISBN 978-0-8493-1983-9. Polakovič M, ... These tight-binding inhibitors may show kinetics similar to covalent irreversible inhibitors. In these cases some of these ...

They impair cell function by forming covalent bonds with the amino, carboxyl, sulfhydryl, and phosphate groups in biologically ... Topoisomerase inhibitors are drugs that affect the activity of two enzymes: topoisomerase I and topoisomerase II. When the DNA ... A Departure from Preformed Organometallic Reagents. Topics in Current Chemistry. Vol. 279. Springer Science & Business Media. ... Trilaciclib is an inhibitor of cyclin-dependent kinase 4/6 approved for the prevention of myelosuppression caused by ...