Collagen-Poly(N-isopropylacrylamide) Hydrogels with Tunable Properties. - The Kennedy Institute of Rheumatology
Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds ... We have demonstrated that by combining collagen with a thermoresponsive polymer, poly(N-isopropylacrylamide) (PNIPAM), the ... Acrylic Resins, Collagen, Cross-Linking Reagents, Humans, Hydrogels, Mechanical Phenomena, Mesenchymal Stem Cells, Tensile ... Biological hydrogels, such as collagen, gelate at physiological temperatures; however, they are not always ideal as scaffolds ...
Imaging and modeling collagen architecture from the nano to micro scale. - The Kennedy Institute of Rheumatology
... and developed a numerical model to quantitatively interpret the images in terms of the underlying collagen structure on the ... The collagen meshwork plays a central role in the functioning of a range of tissues including cartilage, tendon, arteries, skin ... Imaging and modeling collagen architecture from the nano to micro scale. Brown CP., Houle M-A., Popov K., Nicklaus M., Couture ... The collagen meshwork plays a central role in the functioning of a range of tissues including cartilage, tendon, arteries, skin ...
Collagen-induced arthritis in mice. - The Kennedy Institute of Rheumatology
The development of collagen-induced arthritis is associated with strong T- and B-cell responses to type II collagen and the ... Arthritis is normally observed about 3 wk after immunization with autologous or heterologous type II collagen in complete ... are abundantly expressed in the arthritic joints of mice with collagen-induced arthritis and, as in human rheumatoid arthritis ... Collagen-induced arthritis in mice has been widely used to address questions of disease pathogenesis and to validate ...
Analysis of MMP-dependent cell migration and invasion. - The Kennedy Institute of Rheumatology
Animals, Biological Assay, Cell Line, Cell Movement, Collagen, Drug Combinations, Laminin, Matrix Metalloproteinases, ...
Cyclic mechanical load causes global translational arrest in articular chondrocytes: a process which is partially dependent...
Some medium from loaded and unloaded explants was immunoblotted for type II collagen, CTGF and TIMP3. The pathways that control ... Some medium from loaded and unloaded explants was immunoblotted for type II collagen, CTGF and TIMP3. The pathways that control ...
ANTI-CD3 therapy expands the numbers of CD4+ and CD8+ Treg cells and induces sustained amelioration of collagen-induced...
METHODS: Arthritis was induced in DBA/1 mice by immunization with type II collagen. After disease onset, a single injection of ... using collagen-induced arthritis as an animal model. ... using collagen-induced arthritis as an animal model. METHODS: ... ANTI-CD3 therapy expands the numbers of CD4+ and CD8+ Treg cells and induces sustained amelioration of collagen-induced ... ANTI-CD3 therapy expands the numbers of CD4+ and CD8+ Treg cells and induces sustained amelioration of collagen-induced ...
NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I -...
NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I ... NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I ...
Analyses of MT1-MMP activity in cells. - The Kennedy Institute of Rheumatology
Cellular MT1-MMP activity can also be directly detected by in situ degradation of layers of either gelatin or collagen. By ... Arthritis, Rheumatoid, Blotting, Western, Cell Line, Collagen Type I, Dipeptides, Enzyme Activation, Enzyme Precursors, ... Cellular MT1-MMP activity can also be directly detected by in situ degradation of layers of either gelatin or collagen. By ...
Increased limb involvement in murine collagen-induced arthritis following treatment with anti-interferon-gamma. - The Kennedy...
Mice were immunized with human type II collagen in adjuvant on day 1 and boosted with soluble collagen on day 21. H22 was ... or level of anti-type II collagen IgG. However, a highly significant increase (P , 0.01) in the number of limbs affected by ... Mice were immunized with human type II collagen in adjuvant on day 1 and boosted with soluble collagen on day 21. H22 was ... We have tested the effect of administering H22, a hamster neutralizing MoAb to murine interferon-gamma (IFN-gamma) in collagen- ...
Relationship between Th1/Th2 cytokine patterns and the arthritogenic response in collagen-induced arthritis. - The Kennedy...
Murine collagen-induced arthritis (CIA) is an example of an autoimmune disease in which immunization with cartilage-derived ... type II collagen induces, firstly, a T cell response to type II collagen and, secondly, the manifestation of a destructive ... from collagen-immunized DBA/1 mice during the induction phase of arthritis and throughout the time of clinical manifestation ... IFN-gamma was detected in LNC cultures as early as 6 days after immunization and the addition of type II collagen to the ...
SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of...
To investigate the role of SIGIRR in vivo, zymosan-induced arthritis (ZIA) and collagen antibody-induced arthritis (CAIA) were ... To investigate the role of SIGIRR in vivo, zymosan-induced arthritis (ZIA) and collagen antibody-induced arthritis (CAIA) were ...
Protocol for the induction of arthritis in C57BL/6 mice. - The Kennedy Institute of Rheumatology
... and B-cell responses to type II collagen. Key aspects of the protocol are the need to use chicken collagen for immunization and ... The incidence of arthritis varies from 50 to 80% and is milder than the classical collagen-induced arthritis model. This ... This protocol involves purification of type II collagen from chicken sternums, immunization of mice, clinical assessment of ... Collagen-induced arthritis is a well-validated, but strain-dependent mouse model of rheumatoid arthritis, with H-2(q) and H-2(r ...
New grafts for old? A review of alternatives to autologous skin. - The Kennedy Institute of Rheumatology
An alternative is a collagen matrix populated by allogeneic fibroblasts and overlaid with cultured autologous or allogeneic ... A cross-linked collagen and glycosaminoglycan dermal substitute, covered with thin split-skin grafts or cultured autologous ... A cross-linked collagen and glycosaminoglycan dermal substitute, covered with thin split-skin grafts or cultured autologous ... An alternative is a collagen matrix populated by allogeneic fibroblasts and overlaid with cultured autologous or allogeneic ...
IL-33 promotes airway remodeling in pediatric patients with severe steroid-resistant asthma. - The Kennedy Institute of...
IL-33 promoted collagen synthesis both from asthmatic fibroblasts from pediatric patients and after intranasal administration ... and collagen production by airway fibroblasts from pediatric patients stimulated with IL-33 and budesonide was quantified. ... together with collagen production after IL-33 administration. The effect of steroid therapy on IL-33 levels in patients with ... IL-33 promoted collagen synthesis both from asthmatic fibroblasts from pediatric patients and after intranasal administration ...
Cytokine expression and networks in rheumatoid arthritis: rationale for anti-TNF alpha antibody therapy and its mechanism of...
This concept was tested successfully in collagen induced arthritis in mice and led to clinical trials of anti-TNF alpha ... This concept was tested successfully in collagen induced arthritis in mice and led to clinical trials of anti-TNF alpha ...
Williams Group | Targeting Disease Pathways in Rheumatoid Arthritis - The Kennedy Institute of Rheumatology
Blockade of tumor necrosis factor in collagen-induced arthritis reveals a novel immunoregulatory pathway for Th1 and Th17 cells ... Indoleamine 2,3 dioxygenase-mediated tryptophan catabolism regulates accumulation of Th1/Th17 cells in the joint in collagen- ... receptor I blockade is antiinflammatory and reveals immunoregulatory role of tumor necrosis factor receptor II in collagen- ...
NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I -...
NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I ... NOVEL MODELS OF LATE ONSET, PROGRESSIVE OSTEOARTHRITIS CAUSED BY POINT MUTATIONS IN THE TWO ALPHA CHAINS OF COLLAGEN TYPE I ...
Signal Integration in Immune Regulatory Networks - The Kennedy Institute of Rheumatology
To address those questions, we have established a 3D collagen-based culture that supports the migration of human immune cells. ... To address those questions, we have established a 3D collagen-based culture that supports the migration of human immune cells. ...
Comparative analysis of the noncollagenous NC1 domain of type IV collagen: identification of structural features important for...
We suggest that an ancestral NC1 may have been incorporated into type IV collagen as an evolutionarily mobile domain carrying ... The inferred tree verified that type IV collagen sequences fall into two groups, alpha1-like and alpha2-like, and suggested ... sequences and may play a role in the stoichiometric chain selection upon type IV collagen assembly. Neural network secondary ... Type IV collagen alpha1-alpha6 chains have important roles in the assembly of basement membranes and are implicated in the ...
Comparative analysis of the noncollagenous NC1 domain of type IV collagen: identification of structural features important for...
We suggest that an ancestral NC1 may have been incorporated into type IV collagen as an evolutionarily mobile domain carrying ... The inferred tree verified that type IV collagen sequences fall into two groups, alpha1-like and alpha2-like, and suggested ... sequences and may play a role in the stoichiometric chain selection upon type IV collagen assembly. Neural network secondary ... Type IV collagen alpha1-alpha6 chains have important roles in the assembly of basement membranes and are implicated in the ...
Annexin-1 modulates T-cell activation and differentiation. - The Kennedy Institute of Rheumatology
Interestingly, differentiation of naive T cells in the presence of annexin-1 increased skewing in Th1 cells; in the collagen- ... Interestingly, differentiation of naive T cells in the presence of annexin-1 increased skewing in Th1 cells; in the collagen- ...
CD248/endosialin critically regulates hepatic stellate cell proliferation during chronic liver injury via a PDGF-regulated...
Collagen deposition and α-SMA expression, but not inflammation and neoangiogenesis, was reduced in CD248(-/-) mice compared ... Collagen deposition and α-SMA expression, but not inflammation and neoangiogenesis, was reduced in CD248(-/-) mice compared ... Collagen, Desmin, Fibrosis, Gene Expression, Hepatic Stellate Cells, Humans, Inflammation, Liver, Liver Cirrhosis, Mice, Mice, ...
Inhibition of transfer of collagen-induced arthritis into SCID mice by ex vivo infection of spleen cells with retroviruses...
Serum levels of TNF-R did not correlate with clinical signs, however, lower anti-collagen antibody levels corresponded with ... Collagen-induced arthritis can be transferred into severe combined immunodeficiency (SCID) mice by spleen cells from diseased ... Collagen-induced arthritis can be transferred into severe combined immunodeficiency (SCID) mice by spleen cells from diseased ... Inhibition of transfer of collagen-induced arthritis into SCID mice by ex vivo infection of spleen cells with retroviruses ...
The therapeutic activity of low-dose irradiation on experimental arthritis depends on the induction of endogenous regulatory T...
... activation to prevent and/or to treat experimental arthritis using the collagen-induced arthritis (CIA) model and (2) to ... Animals, Arthritis, Experimental, Cells, Cultured, Collagen, Feasibility Studies, Immune Tolerance, Lymphocyte Activation, ... activation to prevent and/or to treat experimental arthritis using the collagen-induced arthritis (CIA) model and (2) to ...
Multiple antibody reactivities to citrullinated antigens in sera from patients with rheumatoid arthritis: association with HLA...
... alpha-enolase peptide-1 and the C1-epitope of type II collagen (C1(III)). Antibody specificity was confirmed by cross- ... Collagen Type II, Cross Reactions, DNA-Binding Proteins, Female, Fibrinogen, Genotype, HLA-DR Antigens, HLA-DRB1 Chains, Humans ... alpha-enolase peptide-1 and the C1-epitope of type II collagen (C1(III)). Antibody specificity was confirmed by cross- ...
Identification and characterization of 4-[[4-(2-butynyloxy)phenyl]sulfonyl]-N-hydroxy-2,2-dimethyl-(3S...
In vivo, TMI-1 is highly effective in reducing clinical severity scores in mouse prophylactic collagen-induced arthritis (CIA) ... In vivo, TMI-1 is highly effective in reducing clinical severity scores in mouse prophylactic collagen-induced arthritis (CIA) ...
Citrullination-acetylation interplay guides E2F-1 activity during the inflammatory response. - The Kennedy Institute of...
In the murine collagen-induced arthritis model, chromatin-bound E2F-1 in inflammatory cells and consequent cytokine expression ... In the murine collagen-induced arthritis model, chromatin-bound E2F-1 in inflammatory cells and consequent cytokine expression ...
Therapeutic senescence via GPCR activation in synovial fibroblasts facilitates resolution of arthritis. - The Kennedy Institute...
... with increased matrix metalloproteinase expression and reduced collagen production. This biological response is attained by ... with increased matrix metalloproteinase expression and reduced collagen production. This biological response is attained by ...
Labelling proteins through the diet gives new insights into how collagen-rich tissues change as we age - The Kennedy Institute...
Labelling proteins through the diet gives new insights into how collagen-rich tissues change as we age Share Share ... During ageing, cellular and physiological processes naturally decline and this is a particular problem in collagen-rich tissues ... Labelling proteins through the diet gives new insights into how collagen-rich tissues change as we age ... which is a dynamic network composed of collagens and proteins, and collectively known as the matrisome. ...