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*  AID 700514 - Modulation of human recombinant GABA-A alpha1beta1gamma2L receptor expressed in Xenopus laevis oocytes assessed as...
BioAssay record AID 700514 submitted by ChEMBL: Modulation of human recombinant GABA-A alpha1beta1gamma2L receptor expressed in Xenopus laevis oocytes assessed as effect on GABA-induced chloride current at 0.01 uM incubated 60 seconds before addition of GABA measured after 30 seconds at holding potential -70 mV by two-microelectrode voltage clamp technique relative to control.
  https://pubchem.ncbi.nlm.nih.gov/bioassay/700514
*  xBMP-4 - Xenopus laevis bone morphogenetic protein-4 | AcronymAttic
How is Xenopus laevis bone morphogenetic protein-4 abbreviated? xBMP-4 stands for Xenopus laevis bone morphogenetic protein-4. xBMP-4 is defined as Xenopus laevis bone morphogenetic protein-4 rarely.
  https://www.acronymattic.com/Xenopus-laevis-bone-morphogenetic-protein_4-
*  Differential muscle regulatory factor gene expression between larval and adult myogenesis in the frog Xenopus laevis: adult...
During Xenopus laevis metamorphosis, larval-to-adult muscle conversion depends on the differential responses of adult and larval myogenic cells to thyroid hormone. Essential differences in cell growth, differentiation, and hormone-dependent life-or-death fate have been reported between cultured larval (tail) and adult (hindlimb) myogenic cells. A previous study revealed that tail notochord cells suppress terminal differentiation in adult (but not larval) myogenic cells. However, little is known about the differences in expression patterns of myogenic regulatory factors (MRF) and the satellite cell marker Pax7 between adult and larval myogenic cells. In the present study, we compared mRNA expression of these factors between the two types. At first, reverse transcription polymerase chain reaction analysis of hindlimb buds showed sequential upregulation of myf5, myogenin, myod, and mrf4 during stages 50-54, when limb buds elongate and muscles begin to form. By contrast, in the ...
  https://www.semanticscholar.org/paper/Differential-muscle-regulatory-factor-gene-express-Yamane-Nishikawa/b36d7bed0d30b219546d44cd5955cd4a927ce047
*  Different forms of soluble cytoplasmic mRNA binding proteins and particles in Xenopus laevis oocytes and embryos. | JCB
To gain insight into the mechanisms involved in the formation of maternally stored mRNPs during Xenopus laevis development, we searched for soluble cytoplasmic proteins of the oocyte that are able to selectively bind mRNAs, using as substrate radiolabeled mRNA. In vitro mRNP assembly in solution was followed by UV-cross-linking and RNase digestion, resulting in covalent tagging of polypeptides by nucleotide transfer. Five polypeptides of approximately 54, 56 60, 70, and 100 kD (p54, p56, p60, p70, and p100) have been found to selectively bind mRNA and assemble into mRNPs. These polypeptides, which correspond to previously described native mRNP components, occur in three different particle classes of approximately 4.5S, approximately 6S, and approximately 15S, as also determined by their reactions with antibodies against p54 and p56. Whereas the approximately 4.5S class contains p42, p60, and p70, probably each in the form of individual molecules or small complexes, the ...
  http://jcb.rupress.org/content/112/1/1
*  Gautier Lab:Xenopus resources - OpenWetWare
In our laboratory, we use Xenopus laevis, commonly known as the African clawed frog (learn more on wikipedia). Xenopus laevis is a great model system for dissecting the molecular pathways in DNA replication and the maintenance of genomic stability. Cell-free extracts made from Xenopus eggs contain all the proteins necessary to undergo 12 rounds of cell-cycle regulated, semi-conservative DNA replication in the absence of transcription. Events in these extracts are highly synchronous, allowing the analysis of short-lived intermediates and the dissection of signal transduction cascades. Particularly, the function of essential proteins can be addressed by immunodepletion or neutralization of these proteins, coupled to rescue with recombinant proteins. Xenopus cell-free extracts are generated by centrifuging unfertilized Xenopus eggs and isolating the cytoplasmic layer. Female ...
  https://openwetware.org/wiki/Gautier_Lab:Xenopus_resources
*  Overland movement in African clawed frogs ( Xenopus laevis ) : a systematic review
African clawed frogs (Xenopus laevis) are often referred to as 'purely aquatic' but there are many publications which suggest extensive overland movements. Previous reviews which considered the topic have not answered the following questions: (1) is there evidence for overland dispersal in native and invasive ranges; (2) what is the range of distances moved overland; (3) when does overland movement occur; and (4) is there evidence of breeding migratory behaviour? A systematic review was chosen to synthesise and critically analyse all literature on the overland movement in Xenopus laevis. Database searches resulted in 57 documents which revealed a paucity of empirical studies, with 28 containing no data, and 19 having anecdotal content. Overwhelming evidence shows that both native and invasive populations of X. laevis move overland, with well documented examples for several other members of the genus (X. borealis, X. gilli, ...
  http://scholar.sun.ac.za/handle/10019.1/102233
*  JAIRO | In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration
In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regenerationIn vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration ...
  http://jairo.nii.ac.jp/0083/00238503/en
*  African Clawed Frog - Xenopus laevis - Overview - Encyclopedia of Life
Xenopus laevis varies in size; males (45.6 to 97.5 mm) tend to be be smaller than females (57 to 147 mm). Their heads and bodies are depressed and flattened and they have small round eyes on the top of their heads. The skin is smooth and the hind limbs are long and robust. The three inner toes of the large fully webbed feet have small black claws on them. The body color is usually dark-gray to greenish-brown dorsally, and pale ventrally (Trueb 2003).. ...
  http://media.eol.org/pages/1038993/overview
*  AID 630202 - Inhibition of retinal rod CNGA1/CNGB1 expressed in Xenopus laevis oocytes assessed as blockade of cGMP-induced...
BioAssay record AID 630202 submitted by ChEMBL: Inhibition of retinal rod CNGA1/CNGB1 expressed in Xenopus laevis oocytes assessed as blockade of cGMP-induced current at +50 mV holding potential by patch-clamp electrophysiology.
  https://pubchem.ncbi.nlm.nih.gov/bioassay/630202
*  Eukaryotic chromosome replication - Chromosome replication in cell-free systems from Xenopus eggs | Philosophical Transactions...
Cell-free systems from eggs of the frog Xenopus laevis are able to perform most of the acts of eukaryotic chromosome replication in vitro. This now includes the crucial regulatory step of initiation, which had only been achieved for viral systems previously. Purified DNA or nuclei are able to initiate and complete semiconservation replication in egg extracts in vitro (Blow & Laskey, Cell 47, 557-587 (1986)). Replication does not require specialized DNA sequences either in vitro or in microinjected eggs, but in both systems large templates replicate more efficiently than small templates. In some cases replication can re-initiate, excluding the possibility that replication is primed by preexisting primers in the template preparations. When nuclei are replicated in vitro, only one round of replication is observed in a single incubation resembling the single round of replication observed for purified DNA after micro-injection. The mechanism that prevents re-initiation of ...
  http://rstb.royalsocietypublishing.org/content/317/1187/483
*  Xwnt-5A: a maternal Wnt that affects morphogenetic movements after overexpression in embryos of Xenopus laevis | Development
To contribute to an understanding of the roles and mechanisms of action of Wnts in early vertebrate development, we have characterized the normal expression of Xenopus laevis Wnt-5A, and investigated the consequences of misexpression of this putative signalling factor. Xwnt-5A transcripts are expressed throughout development, and are enriched in both the anterior and posterior regions of embryos at late stages of development, where they are found primarily in ectoderm, with lower levels of expression in mesoderm. Overexpression of Xwnt-5A in Xenopus embryos leads to complex malformations distinct from those achieved by ectopic expression of Xwnts −1, −3A, or −8. This phenotype is unlikely to result from Xwnt-5A acting as an inducing agent, as overexpression of Xwnt-5A does not rescue dorsal structures in UV-irradiated embryos, does not induce mesoderm in blastula caps, and Xwnt-5A does not alter the endogenous patterns of expression of goosecoid, Xbra, or ...
  http://dev.biologists.org/content/119/1/97
*  Plus it
Materials. Oocytes were obtained from adult female Xenopus laevis frogs (Blades Biological Ltd., Cowden, Edenbridge, Kent, UK) and cared for in accordance with the Institutional Animal Care and Use Committee guidelines. Genistein, 5-hydroxindole, herbimycin A, ACh, nicotine, choline, MLA, and BAPTA-AM were obtained from Sigma (St. Louis, MO). GTS-21 and staurosporine were purchased from Tocris (London, UK). PP2 and SU6656 were obtained from Biaffin GmbH and Co KG (Kassel, Germany). PNU-120596, TQS, and PNU-282987 were synthesized in-house. All other chemicals and reagents were obtained from Sigma or Fisher Scientific (Essex, UK).. Two-Electrode Voltage-Clamp onX. laevisOocytes.X. laevis oocytes were prepared for electrophysiological experiments as described previously (Briggs et al., 1995; Briggs and McKenna, 1998). In brief, three to four lobes from ovaries of female adult X. laevis frogs were removed and defolliculated ...
  http://molpharm.aspetjournals.org/content/72/3/715
*  Xenopus Xlrbpa protein Summary Report | CureHunter
Xenopus Xlrbpa protein: Xenopus laevis homolog of human TAR-RNA-binding protein; MW 33 kDa; amino acid sequence in first source; GenBank M96370
  http://www.curehunter.com/public/keywordSummaryC107296-Xenopus-Xlrbpa-protein.do
*  Plus it
Molecular Biology. Mutations were introduced into rat neuronal nAChR subunits using the GeneEditor in vitro site-directed mutagenesis system (Promega, Madison, WI). All mutations were confirmed by sequencing. Capped cRNA encoding wild-type and mutant subunits was generated using mMessage mMachine kits (Ambion, Austin, TX).. Preparation of Oocytes and cRNA Injection. Oocytes were surgically removed from mature Xenopus laevis frogs (Nasco, Fort Atkinson, WI). The care and use of X. laevis frogs in this study were approved by the University of Miami Animal Research Committee and met the guidelines of the National Institutes of Health. Follicle cells were removed by treatment with Collagenase B (Roche Diagnostics, Indianapolis, IN) for 2 h at room temperature. Stage V oocytes were injected with 0.5 to 10 ng of each cRNA (at a molar ratio of 1:1) in 15 to 50 nl of water and incubated at 18°C in Barth's saline (88 mM NaCl, 1 mM KCl, 2.4 mM NaHCO3, 0.3 mM CaNO3, ...
  http://molpharm.aspetjournals.org/content/69/1/27
*  Xenopus Tadpole Research | School of Biological Sciences | University of Bristol
We use embryos and young tadpoles of the frog Xenopus to ask how nervous systems work at the cellular level, develop to form the correct connections, and are organised to allow animals to behave. Our methods include: analysis of behaviour, whole-cell patch recording, neuron imaging, network modelling. The young Xenopus tadpole provides a very simple model animal with limited behaviour. It can swim either spontaneously or when touched anywhere on the body. A pineal eye detects dimming which speeds up swimming. The tadpole stops swimming when the head bumps into solid objects and the tadpole sticks to things with mucus secreted by a cement gland on the head. If the tadpole is held it can make stronger struggling movements.. This simple behaviour is coordinated by an extremely basic nervous circuit with very few differentiated classes of neuron. For example, at the time of hatching, the spinal cord appears to have only 8 different classes of neuron but the spinal tadpole can ...
  http://www.bristol.ac.uk/biology/research/behaviour/xenopus/
*  A Xenopus Laevis Research Resource for Immunobiology - Jacques Robert
Xenopus laevis is one of the best ectothermic vertebrate models for studying the phylogeny and ontogeny of the immune system. The evolutionary distance of X. la...
  http://grantome.com/grant/NIH/R24-AI059830-09
*  Xenopus Atlas: tissues: kidney, pronephros image - Xenbase | A Xenopus laevis and Xenopus tropicalis resource
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. ...
  http://www.xenbase.org/anatomy/static/organs/kidney/high/163_red-view.jsp
*  Xenopus Atlas: tissues: kidney, pronephros - Xenbase | A Xenopus laevis and Xenopus tropicalis resource
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari. ...
  http://www.xenbase.org/anatomy/static/organs/kidney/high/50_red-view.jsp
*  kcnab2 Summary [species: Xenopus laevis and Xenopus tropicalis] - Xenbase Gene Catalog
Cytoplasmic potassium channel subunit that modulates the characteristics of the channel-forming alpha-subunits (PubMed:10594054). Contributes to the regulation of nerve signaling, and prevents neuronal hyperexcitability. Promotes expression of the pore-forming alpha subunits at the cell membrane, and thereby increases channel activity. Promotes potassium channel closure via a mechanism that does not involve physical obstruction of the channel pore. Promotes KCNA4 channel closure. Modulates the functional properties of KCNA5. Enhances KCNB2 channel activity. Binds NADPH and has NADPH-dependent aldoketoreductase activity. Has broad substrate specificity and can catalyze the reduction of methylglyoxal, 9,10-phenanthrenequinone, prostaglandin J2, 4-nitrobenzaldehyde, 4-nitroacetophenone and 4-oxo-trans-2-nonenal (in vitro). [-] ...
  http://www.xenbase.org/gene/showgene.do?method=display&geneId=990280
*  Difference between revisions of "Xenopus Genome Project" - Marcotte Lab
Xenopus laevis is an essential model organism in several areas of biology. In addition to the key attributes of these embryos for in vivo imaging, cell-free extracts from Xenopus provide among the most powerful in vitro systems for studies of cell and molecular biology. A complete sequence of the X. laevis genome is an essential resource for accurate identification of peptides for mass-spec analyses, for cloning of an ORFeome, for identifying evolutionarily conserved regulatory regions, and for design of morpholino-oligonucleotides for gene knockdowns. The Wallingford and Marcotte labs have obtained funding from the Texas Institute for Drug and Diagnostic Development (TI3D), in coordination with projects funded by the National Institutes of Health, to begin sequencing of the X. laevis genome. We are primarily working with Scott Hunicke-Smith at the University of Texas Genome Sequencing and Analysis facility, with funding ...
  http://www.marcottelab.org/index.php?title=Xenopus_Genome_Project&diff=1084&oldid=1069
*  Difference between revisions of "Xenopus Genome Project" - Marcotte Lab
Xenopus laevis is an essential model organism in several areas of biology. In addition to the key attributes of these embryos for in vivo imaging, cell-free extracts from Xenopus provide among the most powerful in vitro systems for studies of cell and molecular biology. A complete sequence of the X. laevis genome is an essential resource for accurate identification of peptides for mass-spec analyses, for cloning of an ORFeome, for identifying evolutionarily conserved regulatory regions, and for design of morpholino-oligonucleotides for gene knockdowns. The Wallingford and Marcotte labs obtained funding from the Texas Institute for Drug and Diagnostic Development (TI3D), in conjunction with projects funded by the National Institutes of Health, to begin sequencing of the X. laevis genome. We began the project with Scott Hunicke-Smith at the University of Texas Genome Sequencing and Analysis facility, with funding sufficient ...
  http://www.marcottelab.org/index.php?title=Xenopus_Genome_Project&diff=1536&oldid=1523
*  Xenopus Genome Project - Marcotte Lab
Xenopus laevis is an essential model organism in several areas of biology. In addition to the key attributes of these embryos for in vivo imaging, cell-free extracts from Xenopus provide among the most powerful in vitro systems for studies of cell and molecular biology. A complete sequence of the X. laevis genome is an essential resource for accurate identification of peptides for mass-spec analyses, for cloning of an ORFeome, for identifying evolutionarily conserved regulatory regions, and for design of morpholino-oligonucleotides for gene knockdowns. The Wallingford and Marcotte labs obtained funding from the Texas Institute for Drug and Diagnostic Development (TI3D), in conjunction with projects funded by the National Institutes of Health, to begin sequencing of the X. laevis genome. We began the project with Scott Hunicke-Smith at the University of Texas Genome Sequencing and Analysis facility, with funding sufficient ...
  http://www.marcottelab.org/index.php?title=Xenopus_Genome_Project&oldid=1495
*  Germ layer induction in ESC--following the vertebrate roadmap. - Radcliffe Department of Medicine
Controlled differentiation of pluripotential cells takes place routinely and with great success in developing vertebrate embryos. It therefore makes sense to take note of how this is achieved and use this knowledge to control the differentiation of embryonic stem cells (ESCs). An added advantage is that the differentiated cells resulting from this process in embryos have proven functionality and longevity. This unit reviews what is known about the embryonic signals that drive differentiation in one of the most informative of the vertebrate animal models of development, the amphibian Xenopus laevis. It summarizes their identities and the extent to which their activities are dose-dependent. The unit details what is known about the transcription factor responses to these signals, describing the networks of interactions that they generate. It then discusses the target genes of these transcription factors, the effectors of the differentiated state. Finally, how these same ...
  https://www.rdm.ox.ac.uk/publications/106309
*  AID 431578 - Agonist activity at wild type mouse 5HT3A receptor expressed in Xenopus oocyte by two-electrode voltage patch...
BioAssay record AID 431578 submitted by ChEMBL: Agonist activity at wild type mouse 5HT3A receptor expressed in Xenopus oocyte by two-electrode voltage patch clamp method.
  https://pubchem.ncbi.nlm.nih.gov/bioassay/431578