Single-cell transcriptome and BCR analysis. Single-cell library preparation and sequencing were performed by the Princess ... Designation of cell populations based on transcriptome analysis is shown in Supplemental Fig. 1. (C) Ab binding to SARS-CoV S- ... We used the Seurat R package (21) as the main framework for single-cell transcriptome analysis. Cells with coverage on ,200 ... A) Ag receptor sequences from single-cell transcriptomes of cells enriched for S-protein binding were investigated for ...

... our plan is to perform reaggregation and transplantation experiments along with transcriptome sequencing of female and male TEC ...

Transcriptome analysis of control and ORAI1−/− HEK293-ACE2 cells. (A) Normalized read counts (log2) of SARS-CoV-2 RNA products ... Our transcriptome analysis identified many of these host factors among the DEGs in ORAI1−/− cells (Supplemental Fig. 3). ... To uncover the antiviral defense mechanisms impaired in ORAI1−/− cells, we performed transcriptome analysis by bulk RNA-seq of ... 5C-E). Transcriptome analysis further identified multiple transcription factors known to be involved in antiviral pathways, ...

rnaQUAST: a quality assessment tool for de novo transcriptome assemblies. Bioinformatics 32: 2210-2212. ...

Transcriptome profiles demonstrated a 2-fold increase in ISOC1 expression in Th1* cells (10). The effect of ISOC1 depletion in ...

5, Supplemental Table I). These data suggest that CD11cHI and CD11cLO dMɸs have unique and reproducible transcriptomes that ...

To obtain a broad, unbiased view of the transcriptome of CD4+ T cells from Il2−/− mice, we performed RNA sequencing on ...