A microfluidic platform for generating large-scale nearly identical human microphysiological vascularized tissue arrays
This paper reports a polydimethylsiloxane microfluidic model system that can develop an array of nearly identical human ... Tissue Array Analysis / instrumentation* Grants and funding * RC1 ES018361/ES/NIEHS NIH HHS/United States ... A microfluidic platform for generating large-scale nearly identical human microphysiological vascularized tissue arrays Lab ... The simplicity of this design enabled the development of multiple microtissue arrays (5, 12, and 30 microtissues) by co- ...
Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues
Using human tissue arrays, a systematic profiling of protein expression and subcellular localization was undertaken for the ... Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues Mol Cell Proteomics. 2003 ... distribution and expression of proteins in normal tissues as well as in common cancers and other forms of diseased tissues. ...
microRNA mir-34a | Cancer Genetics Web
Tissue Array Analysis. *DNA Methylation. *Tumor Protein p73. *Bone Cancer. *p53 Protein ... There were 317 differentially expressed genes identified in UTUC tissues, and the systematic bioinformatics analyses indicated ... Results showed miR-34a-5p expression in HCC tissues was significantly lower than in non HCC liver tissues (P < 0.05), but MCM2 ... CDH1 expression in tumor tissue was not significantly different from normal tissue (p = 0.33). We found that expression level ...
TOP1 | Cancer Genetics Web
Pixelated spatial gene expression analysis from tissue.. Nat Commun. 2018; 9(1):202 [PubMed] Article available free on PMC ... including subtractive oligo-based array comparative genomic hybridization (soaCGH) analysis. Karyotyping revealed that CPT-K5 ... Soft Tissue Sarcoma. TOP1 and Soft Tissue Sarcoma. View Publications. 27. Colorectal Cancer. TOP1 and Colonic Neoplasms. View ... Combined analyses of genes within the described functions are correlated with poor outcome. PPI network analyses for each ...
RGS12 - Wikipedia
2000). "Use of representational difference analysis and cDNA arrays for transcriptional profiling of tumor tissue". Ann. N. Y. ... 2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci ... 1997). "Molecular cloning and expression analysis of rat Rgs12 and Rgs14". Biochem. Biophys. Res. Commun. 233 (3): 770-7. doi: ... 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation ...
6 Health and Medicine | Optics and Photonics: Essential Technologies for Our Nation | The National Academies Press
Proteomic Analysis Through Protein and Tissue Arrays. Detecting cancer recurrence early, determining the most appropriate drug ... These tissue arrays are analyzed using laser scanners and automated image analysis of the digital images. Drug interactions and ... High-resolution imaging of the excised tissue and analysis of the size and shape of the cells provide the most precise ... Microfluidics and robotics combined with optics provide the technology to create arrays of tissue samples on slides that can be ...
GLUT1 expression is increased in hepatocellular carcinoma and promotes tumorigenesis - University of Regensburg Publication...
RNA, Messenger/analysis. MESH. Reverse Transcriptase Polymerase Chain Reaction. MESH. Tissue Array Analysis. MESH. ... Immunohistochemical analysis of a tissue microarray of 152 HCC cases revealed a significant correlation between Glut1 protein ... We found significantly higher GLUT1 mRNA expression levels in HCC tissues and cell lines compared with primary human ...
LAB06HM
Analysis of phytoestrogens and polyphenols in plasma, tissue, and urine using HPLC with coulometric array detection. Proc Soc ... While the complete blood count and pregnancy analyses are performed in the MEC laboratory, most analyses are conducted ... Barnes S, Coward L, Kirk M, Sfakianos J. HPLC-mass spectrometry analysis of isoflavones. Proc Soc Exp Biol Med. 1998;217:254- ... Read the Special Sample Weights for this Dataset section below before beginning analysis. ...
Integration of Gene Expression Profile Data to Verify Hub Genes of Patients with Stanford A Aortic Dissection
The two mRNAs expression profiles contained 13 TAD aortic tissues and 11 non-TAD tissues. The two expression profile datasets ... Module analysis revealed that TAD was associated with significant pathways including cell cycle, vascular smooth muscle ... ontology and pathway enrichment of DEGs were performed by DAVID and Kyoto Encyclopedia of Genes and Genomes online analyses, ... We will combine our mRNAs expression profile (6 TAD tissues versus 6 non-TAD tissues) and GSE52093 downloaded from the Gene ...
Comparative analysis of microRNA expression in mouse and human brown adipose tissue | BMC Genomics | Full Text
MiRNA levels were measured using PCR-based miRNA arrays. Results were analysed using ExpressionSuite software with the global ... Bio-informatic analyses was used to predict gene targets followed by Ingenuity Pathway Analysis. We identified 35 mouse BAT- ... From this sub-set bioinformatics analysis suggested a potential role of miR-20a in the control of cell fate and this warrants ... white adipose tissue and skeletal muscle. Following this the BAT-enriched miRNAs predicted to target genes potentially involved ...
World Journal of Gastroenterology - Baishideng Publishing Group
IJMS | Free Full-Text | Advances in Proteomic Techniques for Cytokine Analysis: Focus on Melanoma Research
... and planar antibody arrays), ultrasensitive techniques (Singulex, Simoa, immuno-PCR, proximity ligation/extension assay, ... immunomagnetic reduction assay), to analyses of single cells producing cytokines (ELISpot, flow cytometry, mass cytometry and ... Antibody array-based technologies for cancer protein profiling and functional proteomic analyses using serum and tissue ... Antibody arrays are mostly used in form of bead-based arrays (such as Luminex assays, Austin, TX, USA) or planar arrays ( ...
Gene Expression following Exposure to Celecoxib in Humans: Pathways of Inflammation and Carcinogenesis Are Activated in Tumors...
... patterns of gene expression revealed by clustering analysis of tumor and normal colon tissues probed by oligonucleotide arrays ... Hardwick JC, van Santen M, van den Brink GR, et al: DNA array analysis of the effects of aspirin on colon cancer cells: ... and normal tissue examined by oligonucleotide arrays. Cancer Res 2001;61:3124-3130. ... Carcinogenesis, Celecoxib, Colorectal cancer, Gene expression analyses, Genomic profiling, Inflammation, Cell cycle ...
Biomarker detection using corrected degree of domesticity in hybrid social network feature selection for improving classifier...
Due to the nature of the hybrid method, which includes social network analysis, it is necessary to investigate possible ... Social network analysis is used as a part of a hybrid method, although there are several issues that have arisen when using ... a hybrid feature selection method consisting of several machine learning algorithms in addition to social network analysis with ... patterns of gene expression revealed by clustering analysis of tumor and normal colon tissues probed by oligonucleotide arrays ...
TLR4-mediated skin carcinogenesis is dependent on immune and radioresistant cells
... tissue array analysis; toll-like receptor 2; toll-like receptor 4; toll-like receptor 9; molecular biology; biochemistry, ... Consistently, a human tissue microarray analysis showed that melanoma and colon cancer display an over-expression of TLR4 and ... Consistently, a human tissue microarray analysis showed that melanoma and colon cancer display an over-expression of TLR4 and ...
Aberrant Activation Of Hedgehog Signalling Promotes Cell Migration And Invasion Via Matrix Metalloproteinase-7 In Ovarian...
Multiple gene expression analyses in paraffin-embedded tissues by TaqMan low-density array: Application to hedgehog and Wnt ... MMP-7 expression is abnormally elevated in ovarian cancer tissues and predicts poor clinical prognosis. A. Analysis of MMP-7 ... Gross J, Lapiere C M. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U S A. 1962;48: ... IHC analysis demonstrated abnormally increased Gli2 and MMP-7 expression levels in benign tumours and ovarian cancer tissues. ...
Ek, S.<...
From gene expression analysis to tissue microarrays - A rational approach to identify therapeutic and diagnostic targets in ... Cancer Target Group- molecular and fuctional analysis in lymphoid proliferative disorders Our research focus on finding ... eSSENCE@LU 7:2 - Developing a virtual reality platform for the visualisation and analysis of spatially resolved gene expression ...
Protein expression profiling of endometriosis: Validation of 2-mm tissue microarrays<...
Tissue Array Analysis Medicine & Life Sciences 68% * Estrogen Receptors Medicine & Life Sciences 44% ... N2 - Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of ... AB - Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of ... Tissue microarrays (TMAs) are useful tools for studying protein expression in endometriosis. Tissue microarray analyses of ...
Binge Drinking Affects Male and Female Brains Differently - Neuroscience News
... array analysis was employed on NAc tissue to quantify expression levels of 384 genes in a customized Mouse Mood Disorder array ... Pathway analysis suggests hormone signaling and immune function were altered by binge drinking in females, whereas nerve ... Characterization of the expression differences with Ingenuity Pathway Analysis was used to identify Canonical Pathways, ... with a focus on glutamatergic signaling (3 arrays/group). We identified significant regulation of 50 genes in male mice and 70 ...
6 Promising IVD Technologies that Will Change Cancer Testing
Mass spectrometry, sequencing and array technology, and sophisticated bioinformatic analysis of fixed tissues may be used to ... Many of the tissues and fluids from cancer patients do not provide an adequate number of cells for analysis, so using current ... Mass spectrometry analysis of proteins related to specific disease conditions in formalin-fixed, paraffin-embedded tissue and ... Tissue microarrays (TMAs) are used to analyze DNA, mRNA, and protein expression patterns in situ in a wide range of tissue ...
Inflammation-Related DNA Damage and Cancer Stem Cell Markers in Nasopharyngeal Carcinoma
8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. In conclusion, CD44v6 and ALDH1A1 ... In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. ... higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. Expression ... ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues ...
Tissue - Zeljko Vujaskovic
... storage and analysis of plasma samples and tissues bio... ... Gene array analysis will be performed on tissues obtained from ... storage and analysis of plasma samples and tissues biopsies. Specific aims of this core are: 1) To provide a centralized ... Statistical analysis of these results will be done in the Biostatistics and Information Sciences core. This core will be ... Tissues will be used for for immunohistochemical evaluation of the hypoxia-regulated protein, CAIX (carbonic anhydrase, isoform ...
Mismatch repair gene defects in sporadic colorectal cancer enhance immune surveillance | Oncotarget
Immunohistochemical (IHC) analyses were performed using tissue array procedures. CD80, CD4, CD8, T-bet and Fox-P3 expressions ... In our series, 28 (24.7%) patients had at least one MMR gene deficiency (MMR-D) in CRC tissue at immunohistochemical analysis ( ... Statistical analysis. Statistical analysis was carried out with STATISTICA 5.1 software. The results are presented as mean +/- ... Immunohistochemistry analysis was performed on paraffin-embedded tumor samples from these patients. Analysis of mismatch repair ...
Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cells - CIRM
Human Embryonic Stem Cells differentiate into a wide array of adult tissue types and are thought to be the best hope for future ... This analysis has led to the elucidation of new proteins that mediate Nodal signaling; ones that play a key role in endoderm ... Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cells. Return to Grants ... It is critically important that we know everything about this very specialized tissue if we are to attempt to engineer these ...
Siah: A Promising Anticancer Target | Cancer Research | American Association for Cancer Research
... results from a well-annotated cancer tissue array should ideally be paired up with corresponding gene expression analysis. ... Human breast cancer tissues and cells Downregulation of Siah1 observed in human breast cancer tissue, SIAH1-induced apoptosis ... Human breast cancer tissues and cells Downregulation of Siah1 observed in human breast cancer tissue, SIAH1-induced apoptosis ... Human breast cancer tissues and cells Decreased Siah1 expression in human breast cancer tissue and cells, mirrored by decreased ...
Erik Y. Zhang
From analysis of TSC and LAM related gene expression arrays, we identified many potential biomarkers including most protein ... Those genes are differential expressed highly significantly between LAM nodules and normal tissues and they are subject to GSEA ... Pathology are usually revealed as molecular insights of the diseased tissue. ... network and pathway analysis. We also incorporate bio-pathways with our metabolomics data, and develop our working hypothesis. ...
A metadata-aware application for remote scoring and exchange of tissue microarray images. - Oxford Big Data Institute
... and advances in digital scanning microscopy has enabled the collection of thousands of tissue images. There is a need for ... with other standards-compliant systems.The application has been used for multi-centre scoring of TMA slides composed of tissues ... Humans, Breast Neoplasms, Tissue Array Analysis, Internet, Image Processing, Computer-Assisted, Software, Female ... The use of tissue microarrays (TMA) and advances in digital scanning microscopy has enabled the collection of thousands of ...
CD151 | Cancer Genetics Web
MiR-152 was negatively associated with PVT1 expression in GC tissues. Based on the in silico analysis, we found that PVT1 have ... Tissue Array Analysis. *Cancer Gene Expression Regulation. *Survival Rate. *Cell Adhesion. *Neoplasm Metastasis ... RESULTS: PVT1 was up-regulated in GC tissues than that in the matched normal tissues, and mRNA level of miR-152 was decreased. ... Literature Analysis. Mouse over the terms for more detail; many indicate links which you can click for dedicated pages about ...
Interpathologist Diagnostic Agreement for Non-Small Cell Lung Carcinomas Using Current and Recent Classifications.
Carcinoma squamous cell. Medical search. Definitions
Tissue Array Analysis. The simultaneous analysis of multiple samples of TISSUES or CELLS from BIOPSY or in vitro culture that ... Merkel CellDisease-Free SurvivalAnus NeoplasmsTissue Array AnalysisUrinary Bladder NeoplasmsCarcinoma, EndometrioidLip ... AdjuvantOligonucleotide Array Sequence AnalysisIn Situ Hybridization, FluorescenceTransfectionDNA Mutational AnalysisPolymerase ... Oligonucleotide Array Sequence Analysis. Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, ...