*  Molecular Diagnostic studies and Phylogenetic analysis of Theileria species in Egypt - - ScopeMed.org - Deposit for Medical...
Bovine theileriosis is a destructive disease affect the livestock production in Egypt. In the current study 210 cattle and 37 buffaloes from different localities in Upper Egypt were collected and tested by Tams1 target based polymerase chain reaction (Tams1 PCR) and Reverse Line Blot (RLB) assay that target 18S rRNA gene of Theileria spp. flowed by sequencing and phylogenetic analysis for identification of theileria species in both cattle and buffaloes. Tams-1 PCR confirmed the infection in 46.19% and 8.11% while RLB assay confirmed the infection in 65.24% and 51.35% in cattle and buffaloes, respectively. RLB was used as reference test to evaluate Tams-1 PCR assay as a diagnostic tool used for diagnosis of bovine theileriosis, the sensitivity of this test was (70.80% and 15.79%) in cattle and buffaloes, respectively while its specificity was 100% in both animal s species. The PCR products were hybridized on Biodyne C membrane which containing the spacer-oligonucleotide ...
  http://www.scopemed.org/?mno=178117
*  The Toxoplasma Blog: Enforcing host cell polarity: an apicomplexan parasite strategy towards dissemination
The propagation of apicomplexan parasites through transmitting vectors is dependent on effective dissemination of parasites inside the mammalian host. Intracellular Toxoplasma and Theileria parasites face the challenge that their spread inside the host depends in part on the motile capacities of their host cells. In response, these parasites influence the efficiency of dissemination by altering adhesive and/or motile properties of their host cells. Theileria parasites do so by targeting signalling pathways that control host cell actin dynamics. The resulting enforced polar host cell morphology facilitates motility and invasiveness, by establishing focal adhesion and invasion structures at the leading edge of the infected cell. This parasite strategy highlights mechanisms of motility regulation that are also likely relevant for immune or cancer cell motility ...
  http://toxoplasmaparasite.blogspot.com/2011/08/enforcing-host-cell-polarity.html
*  A longitudinal assessment of the serological response to Theileria parva and other tick-borne parasites from birth to one year...
Tick-borne diseases are a major impediment to improved productivity of livestock in sub-Saharan Africa. Improved control of these diseases would be assisted by detailed epidemiological data. Herewe used longitudinal, serological data to determine the patterns of exposure to Theileria parva, Theileria mutans, Babesia bigemina and Anaplasma marginale from 548 indigenous calves in western Kenya. The percentage of calves seropositive for the first three parasites declined from initial high levels due to maternal antibody until week 16, after which the percentage increased until the end of the study. In contrast, the percentage of calves seropositive for T. mutans increased from week 6 and reached a maximal level at week 16. Overall 423 (77%) calves seroconverted to T. parva, 451 (82%) to T. mutans, 195 (36%) to B. bigemina and 275 (50%) to A. marginale. Theileria parva antibody levels were sustained following infection, in contrast to those of the other three ...
  http://repository.up.ac.za/handle/2263/42078
*  Characteristics of Theileria and their antigens - Immune Response
Sporozoites injected during tick feeding are the infective stage; these then invade the host mononuclear leukocytes (see Figure 2). The target cells for T. parva sporozoites are mainly bovine T cells, while those for T. annulata are B lymphocytes and macrophages. The target cell types for other Theileria species have not been defined. Following entry, sporozoites differentiate into multinucleate schizonts. The. Sporozoites. Sporoblast. Kinete. Sporozoites. Sporoblast. Kinete. Zygote ...
  https://www.barnardhealth.us/immune-response/characteristics-of-theileria-and-their-antigens.html
*  Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan : Acta Parasitologica
Equine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PCR products of 10 randomly selected Theileria equi and all Babesia caballi samples. The overall prevalence rates in analysed region for T. equi and B. caballi were 22.5% and 2.1%, respectively. Possible risk factors (such as location, age, sex and activity) associated with PCR positivity were evaluated. Marked differences were found in prevalence between geographic areas. There was no significant ...
  https://www.degruyter.com/view/j/ap.2016.61.issue-2/ap-2016-0044/ap-2016-0044.xml?format=INT
*  Cell penetrating peptides to dissect host-pathogen protein-protein interactions in Theileria -transformed leukocytes - KAUST...
One powerful application of cell penetrating peptides is the delivery into cells of molecules that function as specific competitors or inhibitors of protein-protein interactions. Ablating defined protein-protein interactions is a refined way to explore their contribution to a particular cellular phenotype in a given disease context. Cell-penetrating peptides can be synthetically constrained through various chemical modifications that stabilize a given structural fold with the potential to improve competitive binding to specific targets. Theileria-transformed leukocytes display high PKA activity, but PKAis an enzyme that plays key roles in multiple cellular processes; consequently genetic ablation of kinase activity gives rise to a myriad of confounding phenotypes. By contrast, ablation of a specific kinase-substrate interaction has the potential to give more refined information and we illustrate this here by describing how surgically ablating PKA interactions with BAD gives precise ...
  http://repository.kaust.edu.sa/kaust/handle/10754/625451
*  Genomic Polymorphism, Sexual Recombination and Molecular Epidemiology of Theileria parva | Dr. David Odongo
Bishop R, Geysen D, Skilton R, Odongo D, Nene V, Allsopp B, Mbogo S, Spooner P, Morzaria S. "Genomic Polymorphism, Sexual Recombination and Molecular Epidemiology of Theileria parva.". In: Theileria. The Netherlands: Kluwer Academic Press; 2002 ...
  https://profiles.uonbi.ac.ke/davidodongo/publications/genomic-polymorphism-sexual-recombination-and-molecular-epidemiology-theile
*  Theileria microti - Wikipedia
Theileria microti is a parasitic blood-borne piroplasm transmitted by deer ticks. T. microti is responsible for the disease human theileriosis, similar to babesiosis, a malaria-like disease which also causes fever and hemolysis. T. microti lives in red blood cells, and is an important transfusion-transmitted infectious organism. Between 2010 and 2014 it caused four out of fifteen (27%) of transfusion-transmitted microbial infections[where?] (the highest of any single organism). An important difference from malaria is that T. microti does not infect liver cells. Additionally, the piroplasm is spread by tick bites (Ixodes scapularis, the same tick that spreads Lyme disease), while the malaria protozoans are spread via mosquito. Finally, under the microscope, the merozoite form of the T. microti lifecycle in red blood cells forms a cross-shaped structure, often referred to as a "Maltese cross", whereas malaria forms more of a diamond ring structure in red blood cells. Until 2006 T. microti was ...
  https://en.wikipedia.org/wiki/Theileria_microti
*  Theileriosis in a reindeer (Rangifer tarandus tarandus) associated with a potentially novel Theileria sp - Garner - 2012 -...
If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team ...
  http://onlinelibrary.wiley.com/doi/10.1111/j.1939-165x.2012.00475.x/pdf
*  Bovine Cells Infected in Vivo with Theileria Annulata Express CD11b, the C3bi Complement Receptor - GOV.UK
Bovine cells from cattle infected with Theileria annulata were phenotyped with monoclonal antibodies recognizing bovine leukocyte antigens. Macroschizont-infected, transformed cell lines prepared from peripheral blood mononuclear cells of cattle, infected with sporozoites, were assessed by flow cytometry; parasitized cells in tissues from infected cattle were examined by immunocytochemical techniques. Co-expression of markers for different cell lineages by the cell lines precluded a definite conclusion as to their phenotypic origins. For, while the pattern of leukocyte antigens expressed by these in vivo-derived schizont-infected cells, which included CD11b, was indicative of a myeloid origin, the possibility that they were NK cells could not be excluded. The monoclonal antibody (MAb) IL-A15, which recognizes CD11b, reacted with a high proportion of parasitized cells in sections of tissues from infected cattle at all stages of acute disease. Mononuclear cells infected with parasites at all ...
  https://www.gov.uk/dfid-research-outputs/bovine-cells-infected-in-vivo-with-theileria-annulata-express-cd11b-the-c3bi-complement-receptor
*  Equine piroplasmosis PCR test
Equine piroplasmosis is caused by the intracellular, haemoprotozoan parasites Theileria equi (formerly known as as Babesia equi, Mehlhorn and Schein, 1998) and Babesia caballi, which are transmitted by ticks of several genera including Boophilus, Hyalomma, Dermacentor and Rhipicephalus. The disease is found in many tropical and subtropical areas. Clinical manifestation of the disease is variable and often includes icterus (jaundice), haemoglobinuria and fever. Both chronic and acute infection can occur. Sub-clinical infected animals are of major concern, as they can be carriers of the organism. The geographic movement of presumably healthy horses may aid in the spread of piroplasmosis. In addition to the fact that sub-clinical piroplasmosis may negatively affect the animal's performance, it has been shown that strenuous exercise, such as that experienced in horse racing, can cause sub-clinical infections to become acute (Hailat et al., 1997). Thus there is a real need for the diagnosis of ...
  http://zoologix.com/horse/Datasheets/Babesia.htm
*  Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes
East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte transformation is a unique cancer-like condition that is reversible with parasite removal. Schizont-infected cell-directed CD8+ cytotoxic T lymphocytes (CTL) constitute the dominant protective bovine immune response after a single exposure to infection. However, the schizont antigens targeted by T. parva-specific CTL are undefined. Here we show the identification of five candidate vaccine antigens that are the targets of MHC class I-restricted CD8+ CTL from immune cattle. CD8+ T cell responses to these antigens were boosted in T. parva-immune cattle resolving a challenge infection and, when used to immunize naïve cattle, induced CTL responses that significantly correlated with survival from a lethal parasite challenge. These data provide a basis for developing a CTL-targeted anti-East Coast fever ...
  https://dspacetest.cgiar.org/handle/10568/1406
*  Check For Adult Ticks and Prevent Theileria - The Dairy Site
NEW ZEALAND – Farmers and Veterinarians must unite against a new Ikeda strain to limit the spread of the blood-borne parasite Theileria, advises Dairy New Zealand.
  http://www.thedairysite.com/news/44281/check-for-adult-ticks-and-prevent-theileria/
*  Theileria parva genomics reveals an atypical apicomplexan genome
The discipline of genomics is setting new paradigms in research approaches to resolving problems in human and animal health. We propose to determine the genome sequence of Theileria parva, a pathogen of cattle, using the random shotgun approach pioneered at The Institute for Genomic Research (TIGR). A number of features of the T. parva genome make it particularly suitable for this approach. The G+C content of genomic DNA is about 31 percent, non-coding repetitive DNA constitute less than 1 percent of total DNA and a framework for the 10-12 Mbp genome is available in the form of a physical map for all four chromosomes. Minisatellite sequences are the only dispersed repetitive sequences identified so far, but they are limited in distribution to 13 of 33 SfiI fragments. Telomere and sub-telomeric non-coding sequences occupy less than 10 kbp at each chromosomal end and there are only two units encoding cytoplasmic rRNAs. Three sets of distinct multicopy sequences encoding ORFs have been ...
  https://cgspace.cgiar.org/handle/10568/28787
*  Factors associated with Theileria parva infections in small-holder dairy farms in Murang'a District, Kenya
The association between serum antibody titres to Theileria parva in calves and potential explanatory factors were examined in a longitudinal observational study that was conducted in 5 cohorts of female calves with a total of 225 calves on 188 smallholder dairy farms. The farms were distributed in five agro-ecological zone (AEZ)-grazing strata namely; Upper Midlands UM 1 zero-grazing, UM 1 open-grazing, UM 2, UM 4 zero-grazing and UM 4 open-grazing. The calves were visited within the first two weeks of life and thereafter at biweekly intervals up to the age of 6 months between March 1995 and August 1996. During each visit, a whole blood sample for sera preparation was collected from each calf. Calf management practices in place on the farm during the visit such as grazing system, housing, and tick control were recorded in closed-format questionnaires. Calf serum antibody titres to T. parva were estimated using the indirect enzyme-linked-immunosorbent assay (ELISA) test and expressed as ...
  https://dspacetest.cgiar.org/handle/10568/29300
*  Rapid quantitative assessment of Theileria infection in ticks | SpringerLink
A simplified method for methyl green pyronin staining is described for Theileria parva and T. annulata in whole salivary glands of Rhipicephalus appendiculatus and Hyalomma anatolicum subspecies respe
  https://link.springer.com/article/10.1007%2FBF02237760
*  Genome Sequence of Theileria parva, a Bovine Pathogen That Transforms Lymphocytes - GOV.UK
Gardner, M.J.; Bishop, R.; Shah, T.; de Villiers, E.P.; Carlton, J.M.; Hall, N.; Ren, Q.; Paulsen, I.T.; Pain, A.; Berriman, M.; Wilson, R.J.M.; Sato, S.; Ralph, S.A.; Mann, D.J.; Xiong, Z.; Shallom, S.J.; Weidman, J.; Jiang, L.; Lynn, J.; Weaver, B.; Shoaibi, A.; Wasawo, D.; Crabtree, J.; Wortman, J.R.; Haas, B.; Angiuoli, S.; Creasy, T.; Lu, C.; Suh, B.; Silva, J.; Utterback, T.; Feldblyum, T.; Pertea, M.; Allen, J.; Taracha, E.L.N.; Salzberg, S.L.; White, O.; Fitzhugh, H.A.; Morzaria, S.; Venter, J.C.; Fraser, C.M.; Nene, V. . Genome Sequence of Theileria parva, a Bovine Pathogen That Transforms Lymphocytes. Science (2005) 309 (5731) 134-137. [DOI: 10.1126/science.1110439]. ...
  https://www.gov.uk/dfid-research-outputs/genome-sequence-of-theileria-parva-a-bovine-pathogen-that-transforms-lymphocytes
*  Our work | MPI - Ministry for Primary Industries. A New Zealand Government Department.
2014: New Zealand celebrated freedom from equine viral arteritis (EVA). EVA is a serious cause of abortion in horses and has significant trade implications for the horse breeding industry. Eradication of EVA was made possible by more than 20 years of work at Wallaceville, testing breeding horses and preventing further spread of the disease. 2013: The MPI laboratory at Wallaceville carried out more than 30,000 tests on a wide range of samples from farm animals to companion animals and other species. Results were used in 286 exotic disease investigation cases, surveillance work, and certifying animals as disease-free for trade. 2013: an epidemic of anaemia in Northland cattle was identified at Wallaceville as being caused by Theileria orientalis Ikeda. This led to a major disease control programme involving MPI and the beef and dairy industries. Wallaceville scientists developed new, faster and more accurate tests for the new strain. This test has now been distributed to commercial veterinary ...
  http://mpi.govt.nz/protection-and-response/laboratories/national-biocontainment-laboratory/our-work/
*  Understanding Tick Outbreak in New Zealand Part Two: Detection and Treatment - The Dairy Site
Ticks need to be controlled to prevent infections, North Island farmers have been warned amid a spate of Theileria orientalis cases to sweep across New Zealand.
  http://www.thedairysite.com/articles/3746/understanding-tick-outbreak-in-new-zealand-part-two-detection-and-treatment/
*  Hematological and serum biochemical reference values and cohort analysis in the gilbert's potoroo (Potorous gilbertii) -...
Hematology and serum biochemistry blood values are tabulated for Australia's most critically endangered mammal, the Gilbert's potoroo (Potorous gilbertii). Significant differences were found between origin (captive or wild individuals) and age (subadult or adult) of animals. Sex, and presence or absence of Treponema infection, had minimal significance on blood values. Typical cell morphology is discussed, and hemoparasite examination identified Theileria spp. and Breinlia spp. Eighty samples were collected from a population of only 35 individuals, reflective of a population census rather than of a study reliant on statistical extrapolation. These reference ranges and findings will assist in the ongoing health management of this critically endangered species.. ...
  http://researchrepository.murdoch.edu.au/id/eprint/3638/
*  Redescription ofBabesia equiLaveran, 1901 asTheileria equiMehlhorn, Schein 1998 | SpringerLink
The horse-parasitizing species Babesia equi Laveran, 1901 was redescribed as Theileria equi Mehlhorn, Schein 1998 and, thus, transferred from one valid genus to another. This transfer was needed since
  https://link.springer.com/article/10.1007%2Fs004360050431
*  Babesiosis in Horses - Symptoms, Causes, Diagnosis, Treatment, Recovery, Management, Cost
Babesiosis is a condition that results from your horse's contact with ticks carrying the infection. The infection is from the protozoa Babesia caballi or Theileria equi. - Wag! (formerly Vetary)
  https://wagwalking.com/horse/condition/babesiosis
*  The Best South American Restaurants in Naha - TripAdvisor
Best South American Restaurants in Naha, Okinawa Prefecture: Find TripAdvisor traveller reviews of Naha South American restaurants and search by price, location, and more.
  https://www.tripadvisor.co.uk/Restaurants-g298224-c35-Naha_Okinawa_Prefecture_Kyushu_Okinawa.html
*  Calflora: Aegilops bicornis
Available: http://www.calflora.org/ (Accessed: Jan 18, 2018). The information on this page comes from diverse sources. None of the links are sponsored. ...
  http://www.calflora.org/cgi-bin/species_query.cgi?where-calrecnum=12179
*  8 Ultimate age-preventing technologies you should know about
Inside all three new Dermaheal products - highest concentrations in Ultra Renewal Cream. You'll want to remember the name TGF-B3. It is probably the most potent human growth factor in existence. And it's incredibly rare/scarce because your body only expresses it once in your lifetime - when you're a baby in the womb.. If you've ever marvelled at how a human foetus grows from just a set of basic cells into a near fully-formed baby in just a few months, you'll appreciate that how advanced the growth factors are that go to work while a baby's in gestation. TGF-B3 is one of these. And it's so powerful that it gives baby the ability to heal without any scarring.. Ever notice how quickly and blemish-free a young child's skin heals? That's because of the residual TGF-B3 that's still present after birth. It disappears completely after a while, your super skin healing abilities are lost forever. Until now. With new Dermaheal, you can recapture that kind of skin-healing power as an adult.. ...
  http://www.lapelle.co.za/8-ultimate-age-preventing-technologies-know/