PLOS Pathogens: Herpesvirus Telomerase RNA(vTR)-Dependent Lymphoma Formation Does Not Require Interaction of vTR with...
Recent studies suggest telomerase RNAs can function independently of the telomerase complex and promote tumor development ... with its two main components telomerase reverse transcriptase and telomerase RNA, plays an important role in telomere ... vTR has its first function early after infection, when it is part of the telomerase complex and contributes to the survival of ... The second function of vTR is independent of telomerase action and essential for formation of solid lymphomas and metastasis. ...http://journals.plos.org/plospathogens/article/related?id=10.1371/journal.ppat.1001073&imageURI=info:doi/10.1371/journal.ppat.1001073.g007
"Many Disease-Associated Variants of hTERT Retain High Telomerase Enzym" by Arthur J. Zaug, Sharon M. Crary et al.
It is possible that a small deficit in telomerase activity is sufficient to cause telomere shortening over many years. ... Alternatively, mutations may perturb functions such as the recruitment of telomerase to telomeres, which are essential in vivo ... Furthermore, recent findings that the human POT1-TPP1 chromosome end-binding protein complex stimulates telomerase activity and ... Surprisingly, many of the previously reported disease-associated hTERTalleles give near-normal telomerase enzyme activity. ...https://scholar.colorado.edu/libr_oafund/9/
Telomeres at a glance | Journal of Cell Science
2007). Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere ... Given the natural low abundance of the telomerase enzyme, more sophisticated methods of telomerase detection are needed to ... the telomerase RNA component (TERC) and dyskerin (Cohen et al., 2007). In humans, telomerase is expressed during the early ... as well as telomerase RNA (Peterson et al., 2001; Stellwagen et al., 2003). Our current understanding of telomerase recruitment ...http://jcs.biologists.org/content/125/18/4173
Molecular genetics of telomeres and telomerase | Department of Biology
The correlation between telomerase activity and tumor growth has spurred investigations of the possiblities to use telomerase ... Start › Research › Research groups › Genetics: Telomeres and Telomerase › Molecular genetics of telomeres and telomerase ... The mechanism of telomere elongation by telomerase. In this example the telomerase enzyme is synthesizing the repeated sequence ... Normal human somatic cells lack detectable telomerase activity, whereas telomerase is activated in germ cells, immortalized ...https://www.biology.lu.se/research/research-groups/genetics-telomeres-and-telomerase/molecular-genetics-of-telomeres-and-telomerase
In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits. - Semantic Scholar
The telomerase consists of the catalytic protein subunit TERT and the RNA component TR that provides the template for telomeric ... We assembled the TRBD-CR4/5 ribonucleoprotein complex of the medaka fish telomerase in vitro and determined its atomic ... In vitro reconstitution of telomerase core components in large quantity is the prerequisite to studying the catalytic ... mechanisms of telomerase at the structural level; however, large-scale preparation of recombinant telomerase, especially that ...https://www.semanticscholar.org/paper/In-Vitro-Preparation-and-Crystallization-of-Verteb-Huang-Bley/addd8997771d475f1b9bde47d5f3a62cd316cda4
Patent US6689887 - Inhibition of human telomerase by a G-quadruplex-interaction compound - Google Patents
... that may be associated with human telomeres or telomerase have been identified. Inhibition of human telomerase by two ... acid diimide compounds and provided evidence that these and structurally related compounds inhibit the telomerase enzyme by a ... Certain non-nucleoside compounds that will selectively inhibit telomerase by targeting the nucleic add structures, such as G- ... The telomerase inhibitors of the invention can also be used to inhibit telomerase activity in germ line cells, which may be ...http://www.google.com/patents/US6689887?dq=7565338
Characterisation of telomerase immortalised normal human oesophageal squamous cells | Gut
Telomerase activity was lost by the third passage however. A similar loss of telomerase activity has been reported for squamous ... Telomerase activity and telomere length analysis. Telomerase activity was measured in the original biopsies and in cultured ... Telomere length and telomerase activity in normal oesophageal squamous cells before and after human telomerase (hTERT). (A) ... Results: Cultures of uninfected oesophageal cells had weak telomerase activity at baseline but exhibited loss of telomerase ...http://gut.bmj.com/content/52/3/327
CiNii 論文 - Facile Assay of Telomerase Activity Utilizing a DNA-detectable Chemiluminogenic...
The telomerase substrate was incubated with the cell lysate containing various amounts of telomerase, and then the telomerase ... The telomerasesubstrate was incubated with the cell lysate containing various amounts of telomerase, and then the telomerase ... Facile Assay of Telomerase Activity Utilizing a DNA-detectable Chemiluminogenic Reagent * * Tonooka Keiko TONOOKA Keiko ... Telomerase shows increased activity in most human cancers and germ line cells, but not in normal human somatic cells. We ...http://ci.nii.ac.jp/naid/10021099521
Frontiers | Evolution of TERT-interacting lncRNAs: expanding the regulatory landscape of telomerase | Genetics
One is a conventional telomerase template. The other lncRNA negatively regulates telomerase activity in response to DNA damage ... One is a conventional telomerase template. The other lncRNA negatively regulates telomerase activity in response to DNA damage ... One of the few lncRNAs with a conserved role throughout eukaryotes is the telomerase RNA, TER. TER works in concert with the ... TER works in concert with the telomerase reverse transcriptase (TERT) to maintain telomeres. Here we discuss recent findings ...https://www.frontiersin.org/articles/10.3389/fgene.2015.00277/full
Rapid Quantification of Telomerase Activity Employing an Improved Real-time Telomeric Repeat Amplification Protocol in Clinical...
Telomerase, a ribonucleoprotein with reverse transcriptase activity, enables human cells to maintain chromosomal stability and ... Finally, telomerase activity was expressed as relative telomerase activity (RTA) compared to 293T standards, i.e. the number of ... Telomerase inactivation. To generate negative controls, telomerase was inactivated in diluted samples (1000 ng protein per 50 ... The assay may be employed to detect telomerase activity during carcinogenesis and to monitor telomerase activity during cancer ...https://www.omicsonline.org/rapid-quantification-of-telomerase-activity-employing-an-improved-real-time-telomeric-repeat-amplification-1948-5956.1000084.php?aid=2044
Ultraviolet Irradiation Up-Regulates Telomerase Transcription | IOVS | ARVO Journals
Telomerase activity has been shown to be regulated at the transcriptional and post-translational levels in other telomerase ... the catalytic subunit of telomerase, and possibly up-regulate TR, the RNA component of telomerase. ... We are continuing to evaluate the mechanisms by which telomerase is regulated in normal and cataractous LEC. ... However, we had not evaluated telomerase gene transcription. Therefore, we hypothesized that an acute dose of UVR followed by ...http://iovs.arvojournals.org/article.aspx?articleid=2393884
Role of telomerase in radiocurability (Review)
One phenotypic manifestation that is virtually pathognomonic of several cancer cells is the telomerase activity. Telomerase ... Telomerase activity is present in unicellular organisms and germ cells, both places where it is expected to play a role in ... Telomerase is a ribonucleoprotein complex that elongates telomeres by adding hexameric (TTAGGG) repeats to the telomeric ends ... As tumor growth is partly due to deregulated cell cycling, insights into telomerase activity through the cell cycle may prove ...https://www.spandidos-publications.com/or/10/2/263
Antitumor Activity of Systemically Delivered Ribozymes Targeting Murine Telomerase RNA | Clinical Cancer Research
Telomerase contains essential RNA [Ref. 3 ; telomerase RNA (TER)] and protein [telomerase reverse transcriptase (TERT)] ... Zhang W, Piatyszek MA, Kobayashi T, et al Telomerase activity in human acute myelogenous leukemia: inhibition of telomerase ... components (4 , 5) . The importance of telomerase in cancer is supported by studies showing that telomerase expression promotes ... Inhibition of telomerase activity by a hammerhead ribozyme targeting the RNA component of telomerase in human melanoma cells. J ...http://clincancerres.aacrjournals.org/content/10/15/4983
Telomeres and their associated factors in Arabidopsis thaliana
Unexpectedly, I found plants lacking telomerase for seven generations can lengthen telomeres when telomerase is reintroduced, ... Est1 is a non-catalytic component of the Saccharomyces cerevisiae telomerase holoenzyme. To investigate the role of Est1 in ... Telomeres are maintained by intermittent action of telomerase. I explored the kinetics of telomere length homeostasis in the ... the catalytic subunit of telomerase, and Ku70. Surprisingly, telomere end-to-end fusions were observed in ku70 tert lig4 triple ...https://tdl-ir.tdl.org/tdl-ir/handle/1969.1/4141
Evaluation of intracellular telomerase activity through cascade DNA logic gates - Chemical Science (RSC Publishing) DOI:10...
Telomerase activity of different cell lines. In order to identify the telomerase activity of different cancer cells, a ... This method can work as a useful tool to image telomerase in cancer cells as well as to monitor the response of telomerase to ... Telomerase plays a vital role in cancer and aging, and telomerase activity detection has drawn great attention recently. ... A telomerase substrate (TS) probe, extended by intracellular telomerase, worked as an input to initiate computation cascades. ...http://pubs.rsc.org/en/content/articlehtml/2016/sc/c6sc01953f
ORBi: Browsing ORBi
The stabilization of these structures makes the telomere inaccessible for telomerase and thus inhibits telomerase activity. The ... Proteomic analysis of telomerase inhibition by telomere specific ligands. Mazzucchelli, Gabriel ; Gabelica, Valérie ; ... Telomerase is highly expressed in tumor cells but at very low level in most somatic cells. These observations make the ... Telomerase is an RNA-dependent DNA polymerase that extends telomeres by adding 'TTAGGG' repeats. It consists of a functional ...http://orbi.ulg.ac.be/browse?type=author&value=Riou%2C+Jean-Fran%C3%A7ois
Fine-mapping identifies multiple prostate cancer risk loci at 5p15, one of which associates with TERT expression. - Surgical...
... intronic in the telomerase reverse transcriptase (TERT) gene that encodes TERT. To comprehensively evaluate the association ... intronic in the telomerase reverse transcriptase (TERT) gene that encodes TERT. To comprehensively evaluate the association ...https://www.situ.ox.ac.uk/publications/390878
... Bev A. Kupf bevakupf at ebv.mimnet.northwestern.edu Tue Jul 22 08:38:31 EST 2003 *Previous message: Telomerase ...http://www.bio.net/bionet/mm/methods/2003-July/096615.html
Telomeres and Telomerase | SpringerLink
Telomeres and Telomerase: Methods and Protocols, Second Edition builds upon the telomerase assays featured in the popular first ... Authoritative and practical, Telomeres and Telomerase: Methods and Protocols, Second Edition serves as an ideal, up-to-date ... Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques Eladio Abreu, Rebecca M. Terns, Michael P ... Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In ...https://link.springer.com/book/10.1007/978-1-61779-092-8
Architecture of human telomerase RNA | PNAS
2000) Human telomerase activation requires two independent interactions between telomerase RNA and telomerase reverse ... telomerase reverse transcriptase. Telomerase is a large, multisubunit ribonucleoprotein (RNP) that replicates the 3′ end of ... 2007) Human telomerase RNA accumulation in Cajal bodies facilitates telomerase recruitment to telomeres and telomere elongation ... The telomerase holoenzyme includes a unique reverse transcriptase [telomerase reverse transcriptase (TERT)], an essential RNA ( ...http://www.pnas.org/content/108/51/20325
Telomerase in yeast | Science
The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase ... The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal ... An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the ...http://science.sciencemag.org/content/269/5222/396
Telomeres & Telomerase - QIAGEN
Telomeres and Telomerase RT2 Profiler PCR Array The Rat Telomeres & Telomerase RT² Profiler PCR Array profiles the expression ... Telomeres and Telomerase RT2 Profiler PCR Array The Human Telomeres & Telomerase RT² Profiler PCR Array profiles the expression ... Telomeres and Telomerase RT2 Profiler PCR Array The Mouse Telomeres & Telomerase RT² Profiler PCR Array profiles the expression ... Telomerase (TERT), a reverse transcriptase, forms a complex with an RNA template and cofactors to extend telomeres. The ...https://www.qiagen.com/no/shop/genes-and-pathways/complete-biology-list/telomeres-and-telomerase/
Anti-Aging Telomerase Activation | HubPages
For the first time ever a natural compound has been discovered that activates the enzyme telomerase in the human body. The ... Telomerase Anti-Aging. The enzyme telomerase replenishes telomeres. Telomerase activation in the human body is a requirement ... Telomerase Enzymes Stop Telomeres From Shrinking. It has been proven that the enzyme telomerase can halt the shrinking of ... Telomerase does not stop cancer. Quite the opposite, the research is coming up with ways to inhibit telomerase expression in ...https://hubpages.com/health/Anti-Aging-Telomerase-Activation
Telomerase Is Required for Zebrafish Lifespan
Author Summary Telomerase mutations in humans give rise to premature ageing syndromes. In animals, the wealth of knowledge in ...http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1003214
Phys.org - telomerase
Hyde: Telomere and telomerase in stem cells. When a human cell divides, its daughter cells should each receive an identical ... An enzyme called telomerase plays a significant role in aging and most cancers, but until recently many aspects of the enzyme's ... Cancer researchers at UT Southwestern Medical Center are helping unlock the cellular-level function of the telomerase enzyme, ... a first of its kind atomic level look at the enzyme telomerase ... ...https://phys.org/tags/telomerase/