Preparation of Crude Subcellular Fractions by Differential Centrifugation
... John Graham School of Biomolecular Sciences, ... John Graham, "Preparation of Crude Subcellular Fractions by Differential Centrifugation," TheScientificWorldJOURNAL, vol. 2, pp ...https://www.hindawi.com/journals/tswj/2002/631219/cta/
Biotransformation of some pyrazole derivatives to glutathione conjugates in rat liver subcellular fractions.
... with rat liver microsomaland cytosol fractions in the presence of NADPH-generating system a reactive metabolite, which binds ... with rat liver microsomaland cytosol fractions in the presence of NADPH-generating system a reactive metabolite, which binds ...http://www.biomedsearch.com/nih/Biotransformation-some-pyrazole-derivatives-to/550118.html
Isolation of Subcellular Fractions from the Yeast Saccharomyces cerevisiae - Current Protocols
This unit presents detailed protocols for a range of centrifugation‐based subcellular fractionation procedures for the yeast ... Isolation of subcellular fractions. In Subcellular Fractionation: A Practical Approach (J.M. Graham and D. Rickwood, eds.) pp. ... The unit also contains a table, with references, for alternative approaches to isolation of these organelles and fractions. ... This older review provides an excellent summary of the subcellular fractionation techniques applied to S. cerevisiae, with a ...http://www.currentprotocols.com/WileyCDA/CPUnit/refId-cb0308.html?quicktabs_cp=abstract
The lipid composition of rat brain myelin and subcellular fractions during development | Biochemical Journal
The lipid composition of rat brain myelin and subcellular fractions during development. M. Louise Cuzner, A. N. Davison ... 1. The lipids of whole brain and subcellular fractions of the rat were analysed during development. 2. The deposition of ... The lipid composition of rat brain myelin and subcellular fractions during development ... The lipid composition of rat brain myelin and subcellular fractions during development ...http://www.biochemj.org/content/106/1/29
Evidence of a similar distribution of neutral alpha glucosidase ec 220.127.116.11 and glucose 6 phosphatase in sub cellular fractions...
Evidence of a similar distribution of neutral alpha glucosidase ec 18.104.22.168 and glucose 6 phosphatase in sub cellular fractions ... In subcellular fractions from rat and trout hepatocytes, distribution patterns of neutral .alpha.-glucosidase and Glucose-6- ... Evidence of a similar distribution of neutral alpha glucosidase ec 22.214.171.124 and glucose 6 phosphatase in sub cellular fractions ... Evidence of a similar distribution of neutral alpha glucosidase ec 126.96.36.199 and glucose 6 phosphatase in sub cellular fractions ...https://eurekamag.com/research/005/411/005411163.php
CRYOPRESERVATION OF CELLS AND SUBCELLULAR FRACTIONS - Patent application
Subcellular Fractionation and Pooling  The procedure used to prepare subcellular fractions was based on the method of ... subcellular fractions, such as mitochondria to form a product for creating pooled fractions from different sources via the ... Patent application title: CRYOPRESERVATION OF CELLS AND SUBCELLULAR FRACTIONS. Inventors: Maciej Czerwinski Agents: Assignees: ... Patent application title: CRYOPRESERVATION OF CELLS AND SUBCELLULAR FRACTIONS. Inventors: Maciej Czerwinski (Fairway, KS, US) ...http://www.patentsencyclopedia.com/app/20110105359
Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex and hypothalamus in...
On the level of mitochondrial fractions, the enzyme is involved into the complex of compensatory reactions via glucose-alanin ... mitochondrial and cytosol fractions of visual, orbital, motor, limbic areas of brain cortex and hypothalamus of three-month old ... Agaev, T., Salayeva, E. Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex ... "Dynamics of alaninaminotransferase activity in subcellular fractions of different areas of brain cortex and hypothalamus in ...http://ijpr.sbmu.ac.ir/article_99.html
Isolation of subcellular membrane fractions. Cells were collected, resuspended in PBS solution with Complete-mini EDTA-free ... fraction. When cellular fraction is not used for activity assay, Nuclear (N) and non-nuclear supernatant (NN) was prepared ... D, D3 activity in nuclear or membrane fractions shown in C; data are the mean ± SEM; n = 2 per group; experiments were repeated ... C, D, D3 activity in nuclear (N′) and membrane (M) fractions prepared by centrifugation only; data are the mean ± SEM; n = 2 ...http://www.jneurosci.org/content/32/25/8491
Frontiers | HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3 | Immunology
Subcellular Fractions Extraction. Subcellular fractions were extracted as described (23). A total of 106 HEK293T or A549 cells ... A method for protein extraction from different subcellular fractions of laticifer latex in Hevea brasiliensis compatible with 2 ... HEK293T cells were treated as described above, and the cytoplasm and nuclear fractions were separated. IRF3 phosphorylation ... and the supernatant was stored as the cytoplasm fraction. The pellet was dissolved in 80 µL nucleus extraction buffer and ...https://www.frontiersin.org/articles/10.3389/fimmu.2017.00350/full
The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide-dependent...
Preparation of subcellular fractions. Subcellular fractionation was performed as described with minor modifications (Yang et al ... A systematic survey of subcellular fractions for the presence of histone deacetylase activities led to the detection of a ... Equal amounts of protein from each subcellular fraction were subjected to SDS-PAGE and immunoblotting with an anti-FLAG ... Subcellular localization of hSIRT3 in mitochondria. (A) hSIRT3-GFP was transfected into HeLa cells grown on coverslips. Cells ...http://jcb.rupress.org/content/158/4/647
Targeting to C-Terminal Myosin Heavy Chain May Explain Mechanotransduction Involving Focal Adhesion Kinase in Cardiac Myocytes ...
Subcellular Fractioning. Subcellular fraction enriched in nuclear proteins was obtained from the pellet of left ventricle ... The remaining supernatant fraction that was enriched in soluble, membrane, and myofilament proteins was designated SMM fraction ... Fak subcellular localization was further examined by immunoelectron microscopy. As shown in the representative examples of ... The relative amounts of Fak in SMM and nuclear fractions were estimated by comparison with the amount of Fak from total ...http://circres.ahajournals.org/content/96/1/73
Muscle cell plasma and subcellular fractions were obtained as described by Cushman et al. (13). Briefly, on day 6 after ... Preparation of muscle plasma membrane and subcellular fractions.. GLUT4 protein content in the muscle plasma membrane, low- ... 3D). As expected, insulin increased GLUT4 abundance in plasma membrane fractions, indicating its translocation to the membrane ... protein abundance of GLUT4 in muscle cell membrane fractions was determined (Fig. ...http://diabetes.diabetesjournals.org/content/57/7/1805
Liver Microsomes, S9 Fractions and Cytosol | Thermo Fisher Scientific - JP
... other liver subcellular fractionsLarge pooled lots for reproducible, long-term studiesFractions carefully isolated by experts ... Human microsome pools and subcellular fractions Subcellular fractions, derived from the endoplasmic reticulum of liver, contain ... Table 1 - Relevant applications for liver subcellular fractions Metabolic Enzymes Liver Microsomes Liver S9 Fractions Liver ... Your metabolic tools for drug discovery & development GIBCO® microsomes & other liver subcellular fractions * Large pooled lots ...https://www.thermofisher.com/jp/en/home/industrial/pharma-biopharma/drug-discovery-development/microsomes-s9-fractions-cytosol.html
Regulation of Bax by c-Jun NH2-terminal kinase and Bcl-xL in vinblastine-induced apoptosis
2.3 Preparation of cell extracts and subcellular fractions. For whole cell extracts, cells were lysed for 30 min on ice in ... Mitochondrial and cytosolic fractions were prepared from cells treated with vinblastine either without or with SP600125 pre- ... The integrity of the fractions was confirmed by immunoblotting for Cox II (complex IV) as a mitochondrial marker and for GAPDH ... Vinblastine-induced apoptosis is mediated by discrete alterations in subcellular location, oligomeric structure, and activation ...http://pubmedcentralcanada.ca/pmcc/articles/PMC2692743/
Human and Animal Dermal S9 Fractions now available as standard products. Subcellular fractions are widely used in drug ... Subcellular fractions for ADME studies: broad custom capacities with strong expertise. 26/02/2018. By Isabelle Nobiron Leave a ... In collaboration with Sekisui XenoTech, tebu-bio is now adding human and minipig dermal subcellular fractions to the list of ... New Ready-to-use Dermal Subcellular Fractions. 02/02/2017. By Isabelle Nobiron Leave a Comment ...https://www.tebu-bio.com/blog/category/headlines/adme-tox/
A mitochondrial HSP70 (HSPA9B) is linked to miltefosine resistance and stress response in Leishmania donovani | Parasites &...
Subcellular fractioning and digitonin membrane permeabilization. For subcellular fractioning, the Qproteome Cell Compartment ... b Western blot of subcellular fractions obtained by digitonin fractioning of HSPA9B-mCherry parasites. Samples were separated ... Subcellular fractioned extracts as well as whole protein extracts from logarithmic cultures of L. donovani WT, LdR10.1, LdR30.1 ... Additional evidence of HSPA9B mitochondrial localization was obtained from subcellular fractioning of Leishmania cultures using ...https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-016-1904-8
Search Results - - 58 Results - Digital Library
Effects of Aging on ACTH-Stimulated Steroidogenesis in Subcellular Fractions from Rat Adrenal Glands Description: Young, middle ... In the latter experiment, five corticosteroids were extracted from six different subcellular fractions. Superfused glands ... superfused glands and in their subcellular fractions before and after adrenocorticotropic hormone treatment. ... High cholesterol and progesterone levels in certain old gland fractions were associated with correspondingly reduced 11- ...https://digital.library.unt.edu/search/?q=&fq=str_degree_discipline%3ABiological+Sciences&t=fulltext&fq=dc_type%3Atext_etd
Translational ADMET for Drug Therapy: Principles, Methods, and Pharmaceutical Applications | Drug Discovery & Development |...
188.8.131.52 Subcellular Fractions 93. 184.108.40.206 Cellular Systems 94. 3.5.2 In Vivo Models of Drug Metabolism 95 ...https://www.wiley.com/en-us/Translational+ADMET+for+Drug+Therapy%3A+Principles%2C+Methods%2C+and+Pharmaceutical+Applications-p-9781118838327
Dietary saponins of sea cucumber alleviate orotic acid-induced fatty liver in rats via PPARalpha and SREBP-1c signaling.
Preparation of liver subcellular fractions A piece of liver was homogenized in 6 vol of a 0.25-M sucrose solution containing 1 ... The fraction eluted with 80% ethanol was collected and evaporated, which is crude saponin with a final weight of 51.8 g. ... The entire solution was equilibrated at 30?C. The reaction was initiated by the addition of 0.8 mL cytosol fraction and ... The reaction was initiated by the addition of 0.2 mL mitochondri fraction and absorbance was monitored for 5 min at 412 nm. The ...http://www.biomedsearch.com/nih/Dietary-saponins-sea-cucumber-alleviate/20211032.html
IKZF1 | Cancer Genetics Web
... subcellular localization and protein stability. Recent studies revealed that phosphorylation of Ikaros by CK2 regulates Ikaros ... Subcellular Fractions. *Translocation. *U937 Cells. *Pyrimidines. *Fusion Proteins, bcr-abl. *Childhood Cancer ...http://www.cancerindex.org/geneweb/IKZF1.htm
Human dolichol‐phosphate‐mannose synthase consists of three subunits, DPM1, DPM2 and DPM3 | The EMBO Journal
Isolation of subcellular fractions. CHO and Lec15 cells (3 × 107) were electroporated with 60 μg of pME‐Py‐hDPM3 and 15 μg of ... Fractions were characterized by (A) protein content, (B) organelle‐specific marker enzymes (alkaline phosphodiesterase I for ... The unbound fraction was mixed with glutathione beads and precipitates were collected. Both precipitates were analysed by SDS- ... DPM3 was found by immunoprecipitation and western blotting with anti‐human DPM3 antibodies mainly in fractions 4 and 5, ...http://emboj.embopress.org/content/19/11/2475
Aminopyridines and Similarly Acting Drugs: Effects on Nerves, Muscles and Synapses - 1st Edition
Protein Synthesis in Brain Subcellular Fractions. Effect of 4-Aminopyridine. Effects of 4-Hydroxypyridine on Transmitter ...https://www.elsevier.com/books/aminopyridines-and-similarly-acting-drugs/lechat/978-0-08-028000-4
Functional analysis of Leishmania cyclopropane fatty acid synthetase.
A) Immunoblot analysis of sucrose gradient-separated sub-cellular fractions of wild type L. infantum promastigotes. Fractions ... Sub-cellular fractionation studies locate the C19Δ fatty acid to both ER and plasma membrane-enriched fractions. This fatty ... was detected in Fractions 18-23 of the gradient, with ,80% of the total localising in Fraction 23. (Note that the lower ... Figure 2 Subcellular localization of CFAS-HA in L. infantum. (A) Confocal images of L. infantum promastigotes expressing CFAS- ...http://www.biomedsearch.com/nih/Functional-analysis-leishmania-cyclopropane-Fatty/23251490.html
Endocytic depletion of L-MAG from CNS myelin in quaking mice.
Subcellular Fractions / chemistry. Grant Support. ID/Acronym/Agency: NS29818/NS/NINDS NIH HHS ...http://www.biomedsearch.com/nih/Endocytic-depletion-L-MAG-from/8557747.html
Zeitschrift für Naturforschung C
Differences in the Lipid Distribution in Subcellular Fractions of Mouse Fibroblasts Derived from Logarithmic and Stationary ...https://www.degruyter.com/view/j/znc.1976.31.issue-3-4/issue-files/znc.1976.31.issue-3-4.xml