Cancer Therapeutic Screening in Tumor Spheroids Co-cultured with Fibroblast and Immune Cells Using Corning® Spheroid...
This study demonstrates that the Corning 384-well spheroid microplate can be used to generate and perform viability assays with ... co-culture spheroids in an easy-to-use, high throughput format. ... This study demonstrates that the Corning 384-well spheroid ... Application Note: Cancer Therapeutic Screening in Tumor Spheroids Co-cultured with Fibroblast and Immune Cells Using Corning® ... microplate can be used to generate and perform viability assays with co-culture spheroids in an easy-to-use, high throughput ...http://www.selectscience.net/application-articles/cancer-therapeutic-screening-in-tumor-spheroids-co-cultured-with-fibroblast-and-immune-cells-using-corning--spheroid-microplates/?artID=43767
3D Cell Culture: An Early-Stage Oncology Drug Discovery Tool | SelectScience
This white paper demonstrates the capabilities of the Perfecta3D Hanging Drop Plates from 3D Biomatrix for spheroid growth and ... This white paper demonstrates the capabilities of the Perfecta3D Hanging Drop Plates from 3D Biomatrix for spheroid growth and ...http://www.selectscience.net/application-notes/3d-biomatrix/3d-cell-culture-an-early-stage-oncology-drug-discovery-tool/?artID=27415
Linking hypoxia, DNA damage and proliferation in multicellular tumor spheroids | BMC Cancer | Full Text
6b). Oxygen diffusion into a spheroid is limited by inter-cellular tight junctions and senescent/necrotic cells. Direct ... Spheroids were stained for γ-H2AX after 96 h of inhibitor treatment. Spheroids treated with the ATM inhibitor had fewer γ-H2AX ... These are adjacent sections from the same spheroids imaged in Fig. 3a. c Cell death in A673 spheroids of ,500 μm, 550-650 μm ... Spheroid growth, the onset of hypoxia and necrosis. Spheroids grown with Lewis lung carcinoma (LLC) and Ewing sarcoma (A673) ...https://bmccancer.biomedcentral.com/articles/10.1186/s12885-017-3319-0
Predictions of tumour morphological stability and evaluation against experimental observations | Journal of The Royal Society...
Spheroid growth was observed using a Leitz microscope at magnification 100× over a period of 36 days. Spheroid diameter was ... providing a basis for predicting the underlying cellular conditions from the morphology observed in histopathology (e.g. ... Using the average spheroid radii obtained from the experiments, we first obtain at steady state and then the predicted p values ... Evaluation of tumour spheroid morphological stability in vitro from the experiments in Frieboes et al. . Each entry gives ...http://rsif.royalsocietypublishing.org/content/8/54/16
3D Tumor Spheroid Assays | ProQinase - Drug Discovery in Oncology
ProQinase offers services with a panel of 3D tumor spheroid assays. These 3D assays are designed to measure the effect of test ... Cellular Assay Services. *3D Tumor Spheroid Assays. 3D Tumor Spheroid Assays. Field of Application. This service can be used to ... i) Mono-spheroids with only tumor cells; (ii) co-spheroids with tumor & stroma cells, luciferase measurement only in tumor ... effect of test compounds on three-dimensional cell clones either with mono-spheroids of only tumor cells or with co-spheroids ...https://www.proqinase.com/products-services-cellular-assay-services/3d-tumor-spheroid-assays
Zeiss Light Sheet | W.M. Keck Center for Cellular Imaging, U.Va.
... cell spheroids, embryos (mouse or xenopus), young murine embryos and clarity tissue imaging. ...http://kcci.virginia.edu/facility/zeiss-light-sheet
Corning® Spheroid Microplates: Assay and Analyze 3D Spheroids in the Same Microplate | SelectScience
The Ultra-Low Attachment surface enables uniform and reproducible 3D multicellular spheroid formation. The black opaque ... With their novel and proprietary design, these microplates are ideal for generating and analyzing 3D multicellular spheroids in ... Corning® Spheroid Microplates: Assay and Analyze 3D Spheroids in the Same Microplate. 19 May 2017 ... With their novel and proprietary design, these microplates are ideal for generating and analyzing 3D multicellular spheroids in ...http://www.selectscience.net/SelectScience-TV/Videos/corning-spheroid-microplates-assay-and-analyze-3d-spheroids-in-the-same-microplate/?videoID=3849
tumor spheroids. *tumor morphology instability. *mathematical model. Introduction. Although tissue architecture and cellular ... Typical spheroid cross-sections and curves of spheroid diameter versus time are reported in Fig. 1 . Spheroids showed a rim of ... A) in the spheroid ( 37) and integrating Eq. B over the entire spheroid volume gives the rate of change of the total spheroid ... spheroid and grow into separate tumor spheroids (see photographs in Figs. 3 and 4 ). Thus, unstable glioma spheroids grew as a ...http://cancerres.aacrjournals.org/content/66/3/1597
Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids | Protocol
Nutrient regulation using continuous growth adjusted feeding improves growth rates of mammalian cell spheroids compared to ... For cellular therapies, these techniques could be used in combination with additional regulatory systems to improve cell yield ... The spheroids were observed to increase in size as the culture progresses, and these larger spheroids settled faster due to ... After spheroid formation, divide spheroids among bioreactors with desired culture conditions.. 5. Continuous Feeding Culture ...https://www.jove.com/video/52224/nutrient-regulation-continuous-feeding-for-large-scale-expansion
Maintenance of the spheroid organization and properties of glandular progenitor cells by fabricated chitosan based biomaterials...
Salispheres are spheroid cellular organizations derived from SG stem cells. Benefitting from these cellular organizations, SG ... Salispheres are spheroid cellular organizations derived from SG stem cells. Benefitting from these cellular organizations, SG ... Maintenance of the spheroid organization and properties of glandular progenitor cells by fabricated chitosan based biomaterials ... Maintenance of the spheroid organization and properties of glandular progenitor cells by fabricated chitosan based biomaterials ...http://pubs.rsc.org/en/content/articlelanding/2018/bm/c7bm00559h
Ovarian follicle - Wikipedia
An ovarian follicle is a roughly spheroid cellular aggregation set found in the ovaries. It secretes hormones that influence ...https://en.wikipedia.org/wiki/Ovarian_follicle
Distribution and subcellular localization of a water-soluble hematoporphyrin-platinum(II) complex in human bladder cancer cells...
Spheroids, Cellular. MESH. Tissue Distribution. MESH. Urinary Bladder/metabolism. MESH. Urinary Bladder Neoplasms/drug therapy ... Accumulation studies with 2D and spheroid cell cultures revealed that the concentration of PEG-HPPt was 1.7-times higher in J82 ... Despite its high molecular weight, penetration of PEG-HPPt was not restricted to the peripheral cells of the spheroids. ... Fluorescence microscopic analysis showed that PEG-HPPt was localized in essential cellular targets of photodynamic therapy. DNA ...https://epub.uni-regensburg.de/4827/
Expression levels of stem cell genes and activation of | Open-i
Expression levels of stem cell genes and activation of the Wnt/β-catenin pathway in CD24+ cells.(A) mRNA expression levels of Sox2, Oct4, Nanog, Bmi-1 and Rex-https://openi.nlm.nih.gov/detailedresult.php?img=PMC4067285_pone.0099412.g002&req=4
MeSH Semantic Type: Cell [T025]
Spheroids, Cellular [M0028227]. Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture ... thereby stimulating cellular immunity (IMMUNITY, CELLULAR). When found in the skin they are called LANGERHANS CELLS; when found ... From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4) * Spheroplasts [M0020295]. Cells, usually bacteria or yeast, ... The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive ...http://www.slicksurface.com/medical-thesaurus/semantic-type/cell.htm
CNP-GFP+ postnatal hippocampal neurons are mostly GABAe | Open-i
CNP-GFP+ postnatal hippocampal neurons are mostly GABAergic and receive functional synaptic inputs. Representative confocal images from CA3 (A-C) and dentatehttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2172886_200210110f9&req=4
Anchorage-independent multi-cellular spheroids as an in vitro model of growth signaling in Ewing tumors. Oncogene 2002;21:307- ... Thus, the dramatic cellular changes associated with a hypoxic microenvironment have thus far been largely ignored (31). We ... Growing multicellular spheroids was done essentially as described elsewhere (30). Hypoxic and normoxic treatments of 1 × 105 ... Molecular and Cellular Pathobiology Hypoxia Modulates EWS-FLI1 Transcriptional Signature and Enhances the Malignant Properties ...http://cancerres.aacrjournals.org/content/70/10/4015
Application of mathematics in biology and medicine (Warsawa, 2008). - Contents
Simon D. Angus, Monika J. Piotrowska Towards a 3D cellular automaton model of multi-cellular spheroids ... Wojciech Borkowski Cellular automata model of macroevolution ...................... 18 Katarzyna Buszko, Katarzyna Piecyk, ...http://www.prometeus.nsc.ru/eng/exhibit/09/09-10-06/cont03f.ssi
2009 Lecture 21 - CellBiology
spheroid cellular tissue culture structure. * mouse and human ES cells have the capacity to undergo controlled differentiation ...https://cellbiology.med.unsw.edu.au/cellbiology/index.php/2009_Lecture_21
Three-dimensional multiscale fiber matrices: Development and characterization for increased HepG2 functional maintenance for...
... robust adherence and proliferated growth on the matrix with the formation of characteristic multi-cellular spheroids. Further, ... A comparison study with regard to physico-chemical characterization, hemocompatibility, cytotoxicity, and cellular ...http://pubs.rsc.org/en/content/articlelanding/2018/bm/c7bm00963a
Protocols and Video Articles Authored by Kam W. Leong (Translated to Portuguese)
The attached hepatocytes on PVDF/F68-Gal membrane self-assembled into multi-cellular spheroids after 1 day of culture. These ... May, 2005 , Pubmed ID: 15585256 Primary rat hepatocytes self-assemble into multi-cellular spheroids and maintain differentiated ... The preliminarily cellular mechanisms, from the cellular entry to the endosomal release, were investigated by the correlations ... Co-cultured hepatocyte spheroids exhibited significantly higher liver-specific functions as compared to spheroids cultured ...https://www.jove.com/author/Kam+W._Leong?language=Portuguese
The Female Reproductive System | Boundless Anatomy and Physiology
follicle: A spheroid cellular aggregation found in the ovaries that secretes hormones that influence the stages of the ...https://courses.lumenlearning.com/boundless-ap/chapter/the-female-reproductive-system/
Protocols and Video Articles Authored by Michael D. Menger (Translated to German)
Spheroid sprouting assays are versatile in vitro models for the analysis of molecular and cellular determinants of blood vessel ... Nov, 2017 , Pubmed ID: 28694177 Multi-cellular spheroids mimic the complex three-dimensional environment of natural tissues. ... The cellular uptake of (99m)Tc-FBPBAT was lower at 4 degrees C and 20 degrees C than at 37 degrees C. In contrast, the (123)I- ... Lung Cellular and Molecular Physiology. Jul, 2015 , Pubmed ID: 26024895 Smoking is the main risk factor for the development of ...https://www.jove.com/author/Michael++D._Menger?language=German
DiVA - Search result
Cellular binding was analyzed, after 2 h of incubation, in tumor spheroids composed of BT474 breast cancer cells, which highly ... gamma H2AX and cleaved PARP-1 as apoptotic markers in irradiated breast cancer BT474 cellular spheroids2009In: International ... Breast cancer BT474 cells were grown as cell spheroids to diameters of 700-800 pm. The spheroids contained an outer cell layer ... we have evaluated immunostaining patterns of gamma H2AX and cleaved PARP-1 in paraffin-embedded cellular spheroids. ...http://uu.diva-portal.org/smash/resultList.jsf?af=%5B%5D&aq=%5B%5B%7B%22journalId%22%3A%229326%22%7D%5D%5D&aqe=%5B%5D&aq2=%5B%5B%5D%5D&language=en&query=
Hallmarks in prostate cancer imaging with Ga68-PSMA-11-PET/CT with reference to detection limits and quantitative properties |...
The MDC for LNCaP cells incubated as spheroids (Fig. 2b), is reached at higher cellular number 1.52 × 103, 3.2 × 104, 5 × 104, ... Time course for LNCaP spheroid formation from seeding (a) and at 24 h (b), 48 h (c), 72 h (d), and at 168 h (e). Final spheroid ... 2a. This most likely reflect the in vivo situation as cellular spheroids attain most of the biological characteristics that ... Takagi A, Watanabe M, Ishii Y, Morita J, Hirokawa Y, Matsuzaki T, Shiraishi T. Three-dimensional cellular spheroid formation ...https://link.springer.com/article/10.1186/s13550-018-0378-4
Edible Polymers: Challenges and Opportunities
"Accelerated iron oxide nanoparticle degradation mediated by polyester encapsulation within cellular spheroids," Advanced ...https://www.hindawi.com/journals/jpol/2014/427259/