KAKEN - Research Projects | Molecular analysis of fertilizing ability in human spermatozoa (KAKENHI-PROJECT-07671780)
human spermatozoa / fertilizing capacity / CD46 / acrosome reaction / acrobeads test / 精子 / 受精 / 細胞融合 / 男性不妊. ... To determine the role of CD46 on human spermatozoa, we used anti-CD46 antibodies which recognize different epitopes, short ... These results suggested that SCR-3 of CD46 on human ejaculated spermatozoa may participate in human fertilization.. We also ... We focused on CD46 molecule on human spermatozoa after acrosome reaction. We developed Acrobeads test for diagnosing the ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671780/
KAKEN - Research Projects | A Study of sperm fertilizing ability and intracellular calcium. (KAKENHI-PROJECT-07671769)
The increase in [Ca^2^+]i was observed only in the head of spermatozoon, and there was no [Ca^2^+]i increase in the sperm tail ... Publications] Kamada S: 'Does endothelin-1 affect human spermatozoa function?' Am J Reprod Immunol. 31. 91-98 (1994). *. ... The fluorescence of baseline and peak of the spermatozoa with abnormal morphology was lower when compared with normal sperm. ... Ca^2^+]i of normally shaped spermatozoa increased rapidly after the administration of human follicular fluid. The response ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671769/
KAKEN - Research Projects | Bulk collection of epididymal tail spermatozoa by means of electric stimulation (KAKENHI-PROJECT...
In the condition of low ionic strength, 50% of motile spermatozoa lose motility in the high electric gradient about 160V/2mm. ... In the high ionic strength, spermatozoa lose motility even in low electric gradient but this takes places only near electrode. ... In men with obstructive diseases of the vas deferens, the epididymal spermatozoa could be collected by means of electric ... Publications] Kawakami et al: 'Bulk collection of epididymal tail spermatozoa by means of electric stimulation in rats' Jpn J ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04670973/
KAKEN - Research Projects | Study of Fertillization of the hagfish, Eptatretus burgeri (Agnatha) (KAKENHI-PROJECT-08833013)
The reaction bears resemblance to thet of invertebrate spermatozoa rather than that of vertebrate spermatozoa.. *At the ... was successfully achieved by treatment of mature spermatozoa with ionomycin and excess Ca2+. The spermatozoon produced an ... Such vesicles were observed at the exocytosis of the acrosomal vesicle in both Japanese and Pacific hagfish spermatozoa.. *I ... Publications] S.MORISAWA: 'Exocytosis of the Acrosomal Vesicle of the Hagfish (Eptatretus burgeri) Spermatozoa' Zool.Sei.13. ( ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08833013/
KAKEN - Research Projects | Molecular mechanism of gamete fusion in mammals (KAKENHI-PLANNED-21112006)
Journal Article] Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs2012. *. ... Journal Article] Acrosome-reacted mouse spermatozoa recovered from the perivitelline spacecan fertilize other eggs2011. *. ...https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21112006/
KAKEN - Research Projects | Analysis of flagellar movement of golden hamster spermatozoa by measuring sliding between outer...
... of golden hamster spermatozoa is perhaps different from that of sea urchin spermatozoa.. 2. When the demembranated spermatozoa ... The direction of rolling motion of a spermatozoon about its long axis and of yawing motion of spermatozoa under the coverslip ... In contrast to the sea urchin spermatozoa, the majority of intact golden hamster spermatozoa rolled counterclockwise when ... which is demembranated spermatozoa showing the flagellar movement similar to that of intact spermatozoa when activated with ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640551/
KAKEN - Research Projects | Analysis of the X and Y Chromosomes in Human Spermatozoa Prepared by Swim-up and Percoll Technique ...
Analysis of the X and Y Chromosomes in Human Spermatozoa Prepared by Swim-up and Percoll Technique. Research Project ... Publications] Ping Li,Hiroshi Hoshiai;: 'Detection of Numerical Chromosome Abnormalities in Human Spermatozoa by Three-Color ... Publications] Ping Li, Hiroshi Hoshiai: 'Detection of Numerical Chromosome Abnormalities in Human Spermatozoa by Three-Color ... Publications] Ping Li,Hiroshi Hoshiai: 'Detection of Numerical Chromosome Abnormalities in Human Spermatozoa by Three-Color ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671578/
KAKEN - Research Projects | Molecular mechanisms involved in the sperm maturation. (KAKENHI-PROJECT-05044121)
Publications] Gatti,JL(Dacheux,JL): 'External ionic conditions,internal pH and motility of ram and boar spermatozoa.' J.Reprod. ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044121/
KAKEN - Research Projects | Nuclear transfer in the fowl ovum (KAKENHI-PROJECT-02454094)
The treated ova were incubated at 41 C for 12 h. The same method was used for live spermatozoa. When freeze-thawed spermatozoa ... Hen's ovum / Spermatozoa / Blood nucleus / Microinjection / Egg transfer / 体外受精 / 受精卵挿入 / 排卵 / 精子顕微注入 / 卵割. ... For the injection of live spermatozoa, of 22 treated ova 5 were normal(23%), 7 abnormal and 10 unchanged, From these results it ... Present study was conducted to clear whether the microinjetion of dead or live spermatozoa into the unfertilized ovum may bring ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02454094/
KAKEN - Research Projects | Action of nitric oxide on the mammalian ovary and molecular biological approach on its signal...
Publications] Fujihara N,Inada S,Hasebe M,Yamaguchi H&Hattori M-A: 'In Vivo gene transfer to chick embryos via spermatozoa and ... Publications] Fujihara N,Inada S,Hasebe M,YamaguchiH & Hattori M-A: 'In Vivo gene transfer to chick embryos via spermatozoa and ... In Vivo gene transfer to chick embryos via spermatozoa and primordial germ cells.'Reproductive Biology Update-novel tools for ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09460139/
KAKEN - Research Projects | Mechanisms on Occurrence of Spontaneous Polyploid Fish and Their Application as Resources for...
Both 3n and 5n females had mature eggs, While no male had functional spermatozoa. Offsprings developed from hybridizations (3n* ... 2. Gynogenetic progeny produced from eggs of normal diploid (2n) loach after fertiltzation with UV irradiated spermatozoa died ... of offsprings from 3n and 5n suggest presence of unusual gametogenesis to prevent formation of aneuploidy eggs and spermatozoa ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03806029/
KAKEN - Research Projects | Sperm-Egg binding in ascidian (KAKENHI-PROJECT-09680720)
The spermatozoa bound the vitelline coat in normal sea water (pH 8.2) detached at pH 4. Since the fucosylamine affinity column ... Specific binding of spermatozoa to the vitelline coat is an necessary step for fertilization in various animals. In Halocynthia ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680720/
KAKEN - Research Projects | Molecular Mechanisms Underlying Sperm-Eng Interaction (KAKENHI-PROJECT-09044210)
How Does the Jelly Coat of Starfish Eggs Trigger the Acrosome Reaction in Homologous Spermatozoa? 'The Male Gamete : From Basic ... act in concerted on homologous spermatozoa to elicit the acrosome reaction species-specifically. ARIS is proteoglycan-like ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044210/
KAKEN - Research Projects | Clinical application of seminal plasma sperm motility inhibitor. (KAKENHI-PROJECT-06454461)
... a member of spermadhesin family proteins of boar that bind to spermatozoa. Expression of the boar SPMI gene detected by ...https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06454461/
KAKEN - Research Projects | Elucidating the allorecognition mechanisms in ascidians (prochordates) (KAKENHI-PLANNED-21112002)
We also found that when C. intestinalis spermatozoon binds to the vitelline coat of a self-egg, Ca2+ influx into sperm takes ...https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21112002/